1

.

.

.

.

.

.

.

.

10

.

.

.

.

.

.

.

.

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20

.

.

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.

.

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30

.

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.

.

.

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40

.

.

.

.

.

.

.

.

.

50

.

.

.

.

.

.

.

.

.

60

Sequence

M

A

G

P

L

R

A

P

L

L

L

L

A

I

L

A

V

A

L

A

V

S

P

A

A

G

A

S

P

G

K

P

P

R

L

V

G

G

P

M

D

A

S

V

E

E

E

G

V

R

R

A

L

D

F

A

V

S

E

Y

Secondary structure

SS confidence

Disorder

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

Disorder confidence

Conserved Domain info

•

.

.

.

.

.

.

.

.

.

70

.

.

.

.

.

.

.

.

.

80

.

.

.

.

.

.

.

.

.

90

.

.

.

.

.

.

.

.

.

100

.

.

.

.

.

.

.

.

.

110

.

.

.

.

.

.

.

.

.

120

Sequence

N

K

A

S

N

D

M

Y

H

S

R

A

L

Q

V

V

R

A

R

K

Q

I

V

A

G

V

N

Y

F

L

D

V

E

L

G

R

T

T

C

T

K

T

Q

P

N

L

D

N

C

P

F

H

E

Q

P

H

L

K

R

K

Secondary structure

SS confidence

Disorder

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

Disorder confidence

Conserved Domain info

•

•

•

•

.

.

.

.

.

.

.

.

.

130

.

.

.

.

.

.

.

.

.

140

.

.

.

.

.

.

.

.

.

150

.

.

.

.

.

.

.

.

.

160

.

.

.

.

.

.

.

.

.

170

.

.

.

.

.

.

.

.

.

180

Sequence

A

F

C

S

F

Q

I

Y

T

V

P

W

Q

G

T

M

T

L

S

K

S

T

C

Q

D

A

Q

E

Q

K

L

I

S

E

E

D

L

E

Q

K

L

I

S

E

E

D

L

A

Q

A

E

A

A

A

K

E

A

A

A

K

Secondary structure

SS confidence

Disorder

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

Disorder confidence

Conserved Domain info

.

.

.

.

.

.

.

.

.

190

.

.

.

.

.

.

.

.

.

200

.

.

.

.

.

.

.

.

.

210

.

.

.

.

.

.

.

.

.

220

.

.

.

.

.

.

.

.

.

230

.

.

.

.

.

.

.

.

.

240

Sequence

E

A

A

A

K

E

A

A

A

K

A

L

E

A

E

A

A

A

K

E

A

A

A

K

E

A

A

A

K

E

A

A

A

K

A

A

K

E

A

A

A

K

E

A

A

A

K

E

A

A

V

Q

L

N

T

S

V

Q

I

N

Secondary structure

SS confidence

Disorder

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

Disorder confidence

Conserved Domain info

.

.

.

.

.

.

.

.

.

250

.

.

.

.

.

.

.

.

.

260

.

.

.

.

.

.

.

.

.

270

.

.

.

.

.

.

.

.

.

280

.

.

.

.

.

.

.

Sequence

C

T

R

P

N

Y

N

K

R

K

R

I

H

I

G

R

G

P

G

R

A

F

Y

T

T

G

K

I

G

N

M

R

Q

A

H

C

N

I

S

R

A

K

W

N

N

T

L

Secondary structure

SS confidence

Disorder

?

?

?

?

?

?

?

?

?

?

?

?

?

Disorder confidence

Conserved Domain info

Confidence Key High(9)                     Low (0)

? Disordered ( 41%) Alpha helix ( 33%) Beta strand ( 31%) TM helix ( 6%) (View membrane topology prediction)

CDD sites • putative proteinase inhibition site

Rank Aligned region

PDB 1roa chain A Region: 37 - 145 Aligned: 109 Modelled: 109 Confidence: 100.0% Identity: 56% Fold: Cystatin-like Superfamily: Cystatin/monellin Family: Cystatins Phyre2 PDB 1r4c chain A Region: 37 - 146 Aligned: 110 Modelled: 110 Confidence: 100.0% Identity: 97% Fold: Cystatin-like Superfamily: Cystatin/monellin Family: Cystatins Phyre2 PDB 2ch9 chain A Region: 32 - 145 Aligned: 114 Modelled: 114 Confidence: 100.0% Identity: 33% PDB header:inhibitor Chain: A: PDB Molecule:cystatin f; PDBTitle: crystal structure of dimeric human cystatin f Phyre2 PDB 1cew chain I Region: 37 - 144 Aligned: 108 Modelled: 108 Confidence: 100.0% Identity: 44% Fold: Cystatin-like Superfamily: Cystatin/monellin Family: Cystatins Phyre2 PDB 4it7 chain C Region: 37 - 146 Aligned: 107 Modelled: 110 Confidence: 100.0% Identity: 27% PDB header:hydrolase inhibitor Chain: C: PDB Molecule:cpi; PDBTitle: crystal structure of al-cpi Phyre2 PDB 3mwz chain A Region: 34 - 146 Aligned: 109 Modelled: 113 Confidence: 100.0% Identity: 25% PDB header:hydrolase inhibitor Chain: A: PDB Molecule:sialostatin l2; PDBTitle: crystal structure of the selenomethionine derivative of the l 22,47,2 100 m mutant of sialostatin l2 Phyre2 PDB 3l0r chain A Region: 35 - 145 Aligned: 108 Modelled: 111 Confidence: 100.0% Identity: 23% PDB header:hydrolase inhibitor Chain: A: PDB Molecule:cystatin-2; PDBTitle: crystal structure of salivary cystatin from the soft tick ornithodoros2 moubata Phyre2 PDB 4lzi chain A Region: 30 - 143 Aligned: 92 Modelled: 94 Confidence: 99.9% Identity: 24% PDB header:hydrolase inhibitor Chain: A: PDB Molecule:multicystatin; PDBTitle: characterization of solanum tuberosum multicystatin and significance2 of core domains Phyre2 PDB 2l4v chain A Region: 28 - 142 Aligned: 92 Modelled: 95 Confidence: 99.9% Identity: 30% PDB header:hydrolase inhibitor Chain: A: PDB Molecule:cystatin; PDBTitle: three dimensional structure of pineapple cystatin Phyre2 PDB 3ima chain D Region: 34 - 143 Aligned: 84 Modelled: 90 Confidence: 99.8% Identity: 30% PDB header:hydrolase/hydrolase inhibitor Chain: D: PDB Molecule:cysteine proteinase inhibitor; PDBTitle: complex strcuture of tarocystatin and papain Phyre2 PDB 1eqk chain A Region: 28 - 143 Aligned: 94 Modelled: 96 Confidence: 99.8% Identity: 23% Fold: Cystatin-like Superfamily: Cystatin/monellin Family: Cystatins Phyre2 PDB 2w9p chain C Region: 38 - 143 Aligned: 85 Modelled: 87 Confidence: 99.8% Identity: 20% PDB header:hydrolase inhibitor Chain: C: PDB Molecule:multicystatin; PDBTitle: crystal structure of potato multicystatin Phyre2 PDB 4eyc chain A Region: 49 - 145 Aligned: 91 Modelled: 97 Confidence: 99.8% Identity: 19% PDB header:unknown function Chain: A: PDB Molecule:cathelicidin antimicrobial peptide; PDBTitle: crystal structure of the cathelin-like domain of human cathelicidin2 ll-37 (hcld) Phyre2 PDB 3ul5 chain C Region: 46 - 143 Aligned: 77 Modelled: 79 Confidence: 99.7% Identity: 25% PDB header:hydrolase inhibitor Chain: C: PDB Molecule:canecystatin-1; PDBTitle: saccharum officinarum canecystatin-1 in space group c2221 Phyre2 PDB 2b4c chain G domain 1 Region: 226 - 287 Aligned: 60 Modelled: 62 Confidence: 99.6% Identity: 80% Fold: gp120 core Superfamily: gp120 core Family: gp120 core Phyre2 PDB 1stf chain I Region: 35 - 99 Aligned: 63 Modelled: 65 Confidence: 99.5% Identity: 24% Fold: Cystatin-like Superfamily: Cystatin/monellin Family: Cystatins Phyre2 PDB 3j5m chain I Region: 226 - 287 Aligned: 60 Modelled: 62 Confidence: 99.5% Identity: 72% PDB header:viral protein/immune system Chain: I: PDB Molecule:bg505 sosip gp120; PDBTitle: cryo-em structure of the bg505 sosip.664 hiv-1 env trimer with 3 pgv042 fabs Phyre2 PDB 1ce4 chain A Region: 241 - 276 Aligned: 34 Modelled: 36 Confidence: 99.0% Identity: 85% PDB header:viral protein Chain: A: PDB Molecule:protein (v3 loop of hiv-1 envelope protein); PDBTitle: conformational model for the consensus v3 loop of the2 envelope protein gp120 of hiv-1 Phyre2 PDB 3ngb chain I Region: 226 - 287 Aligned: 42 Modelled: 42 Confidence: 98.7% Identity: 62% PDB header:viral protein/immune system Chain: I: PDB Molecule:envelope glycoprotein gp160; PDBTitle: crystal structure of broadly and potently neutralizing antibody vrc012 in complex with hiv-1 gp120 Phyre2 PDB 1nb5 chain I Region: 35 - 99 Aligned: 63 Modelled: 65 Confidence: 98.6% Identity: 24% Fold: Cystatin-like Superfamily: Cystatin/monellin Family: Cystatins Phyre2 PDB 1y4c chain A Region: 144 - 240 Aligned: 97 Modelled: 97 Confidence: 73.1% Identity: 12% PDB header:de novo protein Chain: A: PDB Molecule:maltose binding protein fused with designed PDBTitle: designed helical protein fusion mbp Phyre2 1 d1roaa_ 2 d1r4ca_ 3 c2ch9A_ 4 d1cewi_ 5 c4it7C_ 6 c3mwzA_ 7 c3l0rA_ 8 c4lziA_ 9 c2l4vA_ 10 c3imaD_ 11 d1eqka_ 12 c2w9pC_ 13 c4eycA_ 14 c3ul5C_ 15 d2b4cg1 16 d1stfi_ 17 c3j5mI_ 18 c1ce4A_ 19 c3ngbI_ 20 d1nb5i_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 c1y4cA_ 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99