Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	K	A	D	N	P	F	D	L	L	L	P	A	A	M	A	K	V	A	E	E	A	G	V	Y	K	A	T	K	H	P	L	K	T	F	Y	L	A	I	T	A	G	V	F	I	S	I	A	F	V	F	Y	I	T	A	T	T	G	T	G
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?																																																	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		T	M	P	F	G	M	A	K	L	V	G	G	I	C	F	S	L	G	L	I	L	C	V	V	C	G	A	D	L	F	T	S	T	V	L	I	V	V	A	K	A	S	G	R	I	T	W	G	Q	L	A	K	N	W	L	N	V	Y	F	G
Secondary structure
SS confidence
Disorder		?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		N	L	V	G	A	L	L	F	V	L	L	M	W	L	S	G	E	Y	M	T	A	N	G	Q	W	G	L	N	V	L	Q	T	A	D	H	K	V	H	H	T	F	I	E	A	V	C	L	G	I	L	A	N	L	M	V	C	L	A	V	W
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		M	S	Y	S	G	R	S	L	M	D	K	A	F	I	M	V	L	P	V	A	M	F	V	A	S	G	F	E	H	S	I	A	N	M	F	M	I	P	M	G	I	V	I	R	D	F	A	S	P	E	F	W	T	A	V	G	S	A	P	E
Secondary structure
SS confidence
Disorder			?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.
Sequence		N	F	S	H	L	T	V	M	N	F	I	T	D	N	L	I	P	V	T	I	G	N	I	I	G	G	G	L	L	V	G	L	T	Y	W	V	I	Y	L	R	E	N	D	H	H
Secondary structure
SS confidence
Disorder																																									?	?	?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 3klz chain E Region: 24 - 280 Aligned: 257 Modelled: 257 Confidence: 100.0% Identity: 55% PDB header:membrane protein Chain: E: PDB Molecule:putative formate transporter 1; PDBTitle: pentameric formate channel with formate bound Phyre2 PDB 3kcv chain G Region: 29 - 280 Aligned: 252 Modelled: 252 Confidence: 100.0% Identity: 100% PDB header:transport protein Chain: G: PDB Molecule:probable formate transporter 1; PDBTitle: structure of formate channel Phyre2 PDB 1lda chain A Region: 30 - 281 Aligned: 236 Modelled: 241 Confidence: 95.6% Identity: 14% PDB header:transport protein Chain: A: PDB Molecule:glycerol uptake facilitator protein; PDBTitle: crystal structure of the e. coli glycerol facilitator (glpf) without2 substrate glycerol Phyre2 PDB 1fx8 chain A Region: 30 - 281 Aligned: 236 Modelled: 241 Confidence: 95.6% Identity: 14% Fold: Aquaporin-like Superfamily: Aquaporin-like Family: Aquaporin-like Phyre2 PDB 3llq chain B Region: 28 - 280 Aligned: 226 Modelled: 229 Confidence: 93.6% Identity: 11% PDB header:membrane protein Chain: B: PDB Molecule:aquaporin z 2; PDBTitle: aquaporin structure from plant pathogen agrobacterium tumerfaciens Phyre2 PDB 2b5f chain D Region: 28 - 277 Aligned: 217 Modelled: 227 Confidence: 91.0% Identity: 11% PDB header:transport protein,membrane protein Chain: D: PDB Molecule:aquaporin; PDBTitle: crystal structure of the spinach aquaporin sopip2;1 in an2 open conformation to 3.9 resolution Phyre2 PDB 1rc2 chain A Region: 32 - 282 Aligned: 226 Modelled: 233 Confidence: 88.9% Identity: 11% Fold: Aquaporin-like Superfamily: Aquaporin-like Family: Aquaporin-like Phyre2 PDB 3iyz chain A Region: 28 - 278 Aligned: 212 Modelled: 226 Confidence: 88.4% Identity: 16% PDB header:transport protein Chain: A: PDB Molecule:aquaporin-4; PDBTitle: structure of aquaporin-4 s180d mutant at 10.0 a resolution from2 electron micrograph Phyre2 PDB 3gd8 chain A Region: 29 - 278 Aligned: 212 Modelled: 225 Confidence: 84.6% Identity: 17% PDB header:membrane protein Chain: A: PDB Molecule:aquaporin-4; PDBTitle: crystal structure of human aquaporin 4 at 1.8 and its mechanism of2 conductance Phyre2 PDB 2w2e chain A Region: 29 - 205 Aligned: 164 Modelled: 164 Confidence: 79.5% Identity: 12% PDB header:membrane protein Chain: A: PDB Molecule:aquaporin; PDBTitle: 1.15 angstrom crystal structure of p.pastoris aquaporin,2 aqy1, in a closed conformation at ph 3.5 Phyre2 PDB 1j4n chain A Region: 28 - 284 Aligned: 220 Modelled: 232 Confidence: 77.9% Identity: 12% Fold: Aquaporin-like Superfamily: Aquaporin-like Family: Aquaporin-like Phyre2 PDB 2d57 chain A Region: 28 - 278 Aligned: 213 Modelled: 225 Confidence: 77.6% Identity: 15% PDB header:transport protein Chain: A: PDB Molecule:aquaporin-4; PDBTitle: double layered 2d crystal structure of aquaporin-4 (aqp4m23) at 3.2 a2 resolution by electron crystallography Phyre2 PDB 3c02 chain A Region: 30 - 283 Aligned: 234 Modelled: 254 Confidence: 73.3% Identity: 9% PDB header:membrane protein Chain: A: PDB Molecule:aquaglyceroporin; PDBTitle: x-ray structure of the aquaglyceroporin from plasmodium falciparum Phyre2 PDB 3d9s chain B Region: 24 - 285 Aligned: 222 Modelled: 237 Confidence: 50.3% Identity: 12% PDB header:membrane protein Chain: B: PDB Molecule:aquaporin-5; PDBTitle: human aquaporin 5 (aqp5) - high resolution x-ray structure Phyre2 PDB 2f2b chain A Region: 30 - 204 Aligned: 167 Modelled: 175 Confidence: 48.5% Identity: 9% PDB header:membrane protein Chain: A: PDB Molecule:aquaporin aqpm; PDBTitle: crystal structure of integral membrane protein aquaporin aqpm at 1.68a2 resolution Phyre2 PDB 1w2z chain A domain 2 Region: 5 - 20 Aligned: 16 Modelled: 16 Confidence: 46.4% Identity: 25% Fold: Cystatin-like Superfamily: Amine oxidase N-terminal region Family: Amine oxidase N-terminal region Phyre2 PDB 1ymg chain A Region: 28 - 284 Aligned: 214 Modelled: 232 Confidence: 46.0% Identity: 14% PDB header:membrane protein Chain: A: PDB Molecule:lens fiber major intrinsic protein; PDBTitle: the channel architecture of aquaporin o at 2.2 angstrom resolution Phyre2 PDB 1ymg chain A domain 1 Region: 28 - 284 Aligned: 214 Modelled: 232 Confidence: 46.0% Identity: 14% Fold: Aquaporin-like Superfamily: Aquaporin-like Family: Aquaporin-like Phyre2 PDB 1d6z chain A domain 2 Region: 2 - 20 Aligned: 19 Modelled: 19 Confidence: 41.9% Identity: 21% Fold: Cystatin-like Superfamily: Amine oxidase N-terminal region Family: Amine oxidase N-terminal region Phyre2 PDB 2oqe chain A domain 2 Region: 5 - 20 Aligned: 16 Modelled: 16 Confidence: 41.3% Identity: 25% Fold: Cystatin-like Superfamily: Amine oxidase N-terminal region Family: Amine oxidase N-terminal region Phyre2 1 c3klzE_ 2 c3kcvG_ 3 c1ldaA_ 4 d1fx8a_ 5 c3llqB_ 6 c2b5fD_ 7 d1rc2a_ 8 c3iyzA_ 9 c3gd8A_ 10 c2w2eA_ 11 d1j4na_ 12 c2d57A_ 13 c3c02A_ 14 c3d9sB_ 15 c2f2bA_ 16 d1w2za2 17 c1ymgA_ 18 d1ymga1 19 d1d6za2 20 d2oqea2 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 c3klzE_ 100.0 55 PDB header:membrane protein Chain: E: PDB Molecule:putative formate transporter 1; PDBTitle: pentameric formate channel with formate bound 2 c3kcvG_ 100.0 100 PDB header:transport protein Chain: G: PDB Molecule:probable formate transporter 1; PDBTitle: structure of formate channel 3 c1ldaA_ 95.6 14 PDB header:transport protein Chain: A: PDB Molecule:glycerol uptake facilitator protein; PDBTitle: crystal structure of the e. coli glycerol facilitator (glpf) without2 substrate glycerol 4 d1fx8a_ 95.6 14 Fold:Aquaporin-like Superfamily:Aquaporin-like Family:Aquaporin-like 5 c3llqB_ 93.6 11 PDB header:membrane protein Chain: B: PDB Molecule:aquaporin z 2; PDBTitle: aquaporin structure from plant pathogen agrobacterium tumerfaciens 6 c2b5fD_ 91.0 11 PDB header:transport protein,membrane protein Chain: D: PDB Molecule:aquaporin; PDBTitle: crystal structure of the spinach aquaporin sopip2;1 in an2 open conformation to 3.9 resolution 7 d1rc2a_ 88.9 11 Fold:Aquaporin-like Superfamily:Aquaporin-like Family:Aquaporin-like 8 c3iyzA_ 88.4 16 PDB header:transport protein Chain: A: PDB Molecule:aquaporin-4; PDBTitle: structure of aquaporin-4 s180d mutant at 10.0 a resolution from2 electron micrograph 9 c3gd8A_ 84.6 17 PDB header:membrane protein Chain: A: PDB Molecule:aquaporin-4; PDBTitle: crystal structure of human aquaporin 4 at 1.8 and its mechanism of2 conductance 10 c2w2eA_ 79.5 12 PDB header:membrane protein Chain: A: PDB Molecule:aquaporin; PDBTitle: 1.15 angstrom crystal structure of p.pastoris aquaporin,2 aqy1, in a closed conformation at ph 3.5 11 d1j4na_ 77.9 12 Fold:Aquaporin-like Superfamily:Aquaporin-like Family:Aquaporin-like 12 c2d57A_ 77.6 15 PDB header:transport protein Chain: A: PDB Molecule:aquaporin-4; PDBTitle: double layered 2d crystal structure of aquaporin-4 (aqp4m23) at 3.2 a2 resolution by electron crystallography 13 c3c02A_ 73.3 9 PDB header:membrane protein Chain: A: PDB Molecule:aquaglyceroporin; PDBTitle: x-ray structure of the aquaglyceroporin from plasmodium falciparum 14 c3d9sB_ 50.3 12 PDB header:membrane protein Chain: B: PDB Molecule:aquaporin-5; PDBTitle: human aquaporin 5 (aqp5) - high resolution x-ray structure 15 c2f2bA_ 48.5 9 PDB header:membrane protein Chain: A: PDB Molecule:aquaporin aqpm; PDBTitle: crystal structure of integral membrane protein aquaporin aqpm at 1.68a2 resolution 16 d1w2za2 46.4 25 Fold:Cystatin-like Superfamily:Amine oxidase N-terminal region Family:Amine oxidase N-terminal region 17 c1ymgA_ 46.0 14 PDB header:membrane protein Chain: A: PDB Molecule:lens fiber major intrinsic protein; PDBTitle: the channel architecture of aquaporin o at 2.2 angstrom resolution 18 d1ymga1 46.0 14 Fold:Aquaporin-like Superfamily:Aquaporin-like Family:Aquaporin-like 19 d1d6za2 41.9 21 Fold:Cystatin-like Superfamily:Amine oxidase N-terminal region Family:Amine oxidase N-terminal region 20 d2oqea2 41.3 25 Fold:Cystatin-like Superfamily:Amine oxidase N-terminal region Family:Amine oxidase N-terminal region 21 d1w6ga2 not modelled 40.6 25 Fold:Cystatin-like Superfamily:Amine oxidase N-terminal region Family:Amine oxidase N-terminal region 22 d1h6ia_ not modelled 21.0 9 Fold:Aquaporin-like Superfamily:Aquaporin-like Family:Aquaporin-like 23 c3e49A_ not modelled 16.1 16 PDB header:metal binding protein Chain: A: PDB Molecule:uncharacterized protein duf849 with a tim barrel fold; PDBTitle: crystal structure of a prokaryotic domain of unknown function (duf849)2 with a tim barrel fold (bxe_c0966) from burkholderia xenovorans lb4003 at 1.75 a resolution 24 c3lotC_ not modelled 15.5 18 PDB header:structure genomics, unknown function Chain: C: PDB Molecule:uncharacterized protein; PDBTitle: crystal structure of protein of unknown function (np_070038.1) from2 archaeoglobus fulgidus at 1.89 a resolution 25 c3c6cA_ not modelled 15.1 24 PDB header:hydrolase Chain: A: PDB Molecule:3-keto-5-aminohexanoate cleavage enzyme; PDBTitle: crystal structure of a putative 3-keto-5-aminohexanoate cleavage2 enzyme (reut_c6226) from ralstonia eutropha jmp134 at 1.72 a3 resolution 26 c2y7eA_ not modelled 13.6 28 PDB header:lyase Chain: A: PDB Molecule:3-keto-5-aminohexanoate cleavage enzyme; PDBTitle: crystal structure of the 3-keto-5-aminohexanoate cleavage enzyme2 (kce) from candidatus cloacamonas acidaminovorans (tetragonal form) 27 d1c17m_ not modelled 13.6 31 Fold:F1F0 ATP synthase subunit A Superfamily:F1F0 ATP synthase subunit A Family:F1F0 ATP synthase subunit A 28 c1ksiA_ not modelled 12.9 25 PDB header:oxidoreductase Chain: A: PDB Molecule:copper amine oxidase; PDBTitle: crystal structure of a eukaryotic (pea seedling) copper-containing2 amine oxidase at 2.2a resolution 29 c3loyB_ not modelled 12.5 20 PDB header:oxidoreductase Chain: B: PDB Molecule:copper amine oxidase; PDBTitle: crystal structure of a copper-containing benzylamine oxidase from2 hansenula polymorpha 30 c3no5C_ not modelled 12.1 20 PDB header:structural genomics, unknown function Chain: C: PDB Molecule:uncharacterized protein; PDBTitle: crystal structure of a pfam duf849 domain containing protein2 (reut_a1631) from ralstonia eutropha jmp134 at 1.90 a resolution 31 c3chvA_ not modelled 11.9 28 PDB header:metal binding protein Chain: A: PDB Molecule:prokaryotic domain of unknown function (duf849) with a tim PDBTitle: crystal structure of a prokaryotic domain of unknown function (duf849)2 member (spoa0042) from silicibacter pomeroyi dss-3 at 1.45 a3 resolution 32 c1d6uB_ not modelled 11.7 21 PDB header:oxidoreductase Chain: B: PDB Molecule:copper amine oxidase; PDBTitle: crystal structure of e. coli amine oxidase anaerobically reduced with2 beta-phenylethylamine 33 c1ui7A_ not modelled 11.5 25 PDB header:oxidoreductase Chain: A: PDB Molecule:phenylethylamine oxidase; PDBTitle: site-directed mutagenesis of his433 involved in binding of2 copper ion in arthrobacter globiformis amine oxidase 34 c1ekmC_ not modelled 10.5 25 PDB header:oxidoreductase Chain: C: PDB Molecule:copper amine oxidase; PDBTitle: crystal structure at 2.5 a resolution of zinc-substituted2 copper amine oxidase of hansenula polymorpha expressed in3 escherichia coli 35 c3nbbC_ not modelled 10.1 25 PDB header:oxidoreductase Chain: C: PDB Molecule:peroxisomal primary amine oxidase; PDBTitle: crystal structure of mutant y305f expressed in e. coli in the copper2 amine oxidase from hansenula polymorpha 36 c3e02A_ not modelled 9.3 16 PDB header:metal binding protein Chain: A: PDB Molecule:uncharacterized protein duf849; PDBTitle: crystal structure of a duf849 family protein (bxe_c0271) from2 burkholderia xenovorans lb400 at 1.90 a resolution 37 c2jy0A_ not modelled 8.5 50 PDB header:membrane protein, viral protein Chain: A: PDB Molecule:protease ns2-3; PDBTitle: solution nmr structure of hcv ns2 protein, membrane segment2 (1-27) 38 d2c1wa1 not modelled 8.2 67 Fold:EndoU-like Superfamily:EndoU-like Family:Eukaryotic EndoU ribonuclease 39 d1rm6b2 not modelled 7.7 25 Fold:FAD-binding/transporter-associated domain-like Superfamily:FAD-binding/transporter-associated domain-like Family:CO dehydrogenase flavoprotein N-terminal domain-like 40 d2foka2 not modelled 7.0 10 Fold:DNA/RNA-binding 3-helical bundle Superfamily:"Winged helix" DNA-binding domain Family:Restriction endonuclease FokI, N-terminal (recognition) domain 41 c1rm6E_ not modelled 6.9 25 PDB header:oxidoreductase Chain: E: PDB Molecule:4-hydroxybenzoyl-coa reductase beta subunit; PDBTitle: structure of 4-hydroxybenzoyl-coa reductase from thauera2 aromatica 42 c2voyG_ not modelled 5.5 39 PDB header:hydrolase Chain: G: PDB Molecule:sarcoplasmic/endoplasmic reticulum calcium PDBTitle: cryoem model of copa, the copper transporting atpase from2 archaeoglobus fulgidus

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

Transmembrane helix prediction

Transmembrane helices have been predicted in your sequence to adopt the topology shown below

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0