Secondary structure and disorder prediction

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Sequence		M	S	S	E	R	D	L	V	N	F	L	G	D	F	S	M	D	V	A	K	A	V	I	A	G	G	V	A	T	A	I	G	S	L	A	S	F	A	C	V	S	F	G	F	P	V	I	L	V	G	G	A	I	L	L	T	G	I	V	C
Secondary structure
SS confidence
Disorder		?	?	?																																								?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.
Sequence		T	V	V	L	N	E	I	D	A	Q	C	H	L	S	E	K	L	K	Y	A	I	R	D	G	L	K	R	Q	Q	E	L	D	K	W	K	R	E	N	M	T	P	F	M	Y	V	L	N	T	P	P	V	I
Secondary structure
SS confidence
Disorder																											?		?			?	?	?	?	?	?	?	?	?		?					?	?	?	?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 1cii chain A Region: 6 - 55 Aligned: 50 Modelled: 50 Confidence: 66.2% Identity: 14% PDB header:transmembrane protein Chain: A: PDB Molecule:colicin ia; PDBTitle: colicin ia Phyre2 PDB 3few chain X Region: 6 - 65 Aligned: 60 Modelled: 60 Confidence: 59.7% Identity: 17% PDB header:immune system Chain: X: PDB Molecule:colicin s4; PDBTitle: structure and function of colicin s4, a colicin with a2 duplicated receptor binding domain Phyre2 PDB 1yqg chain A domain 1 Region: 63 - 96 Aligned: 33 Modelled: 34 Confidence: 51.0% Identity: 12% Fold: 6-phosphogluconate dehydrogenase C-terminal domain-like Superfamily: 6-phosphogluconate dehydrogenase C-terminal domain-like Family: ProC C-terminal domain-like Phyre2 PDB 1col chain A Region: 6 - 65 Aligned: 60 Modelled: 60 Confidence: 45.0% Identity: 17% Fold: Toxins' membrane translocation domains Superfamily: Colicin Family: Colicin Phyre2 PDB 1rh1 chain A domain 2 Region: 6 - 65 Aligned: 60 Modelled: 60 Confidence: 44.9% Identity: 10% Fold: Toxins' membrane translocation domains Superfamily: Colicin Family: Colicin Phyre2 PDB 1rh1 chain A Region: 6 - 65 Aligned: 60 Modelled: 60 Confidence: 28.1% Identity: 10% PDB header:antibiotic Chain: A: PDB Molecule:colicin b; PDBTitle: crystal structure of the cytotoxic bacterial protein2 colicin b at 2.5 a resolution Phyre2 PDB 1uxc chain A Region: 60 - 87 Aligned: 28 Modelled: 28 Confidence: 27.5% Identity: 18% Fold: lambda repressor-like DNA-binding domains Superfamily: lambda repressor-like DNA-binding domains Family: GalR/LacI-like bacterial regulator Phyre2 PDB 2ag8 chain A Region: 63 - 96 Aligned: 33 Modelled: 34 Confidence: 21.6% Identity: 12% PDB header:oxidoreductase Chain: A: PDB Molecule:pyrroline-5-carboxylate reductase; PDBTitle: nadp complex of pyrroline-5-carboxylate reductase from neisseria2 meningitidis Phyre2 PDB 3mb2 chain J Region: 88 - 110 Aligned: 23 Modelled: 23 Confidence: 20.4% Identity: 26% PDB header:isomerase Chain: J: PDB Molecule:4-oxalocrotonate tautomerase family enzyme - beta subunit; PDBTitle: kinetic and structural characterization of a heterohexamer 4-2 oxalocrotonate tautomerase from chloroflexus aurantiacus j-10-fl:3 implications for functional and structural diversity in the4 tautomerase superfamily Phyre2 PDB 3qwm chain A Region: 74 - 103 Aligned: 30 Modelled: 30 Confidence: 17.5% Identity: 20% PDB header:signaling protein Chain: A: PDB Molecule:iq motif and sec7 domain-containing protein 1; PDBTitle: crystal structure of gep100, the plextrin homology domain of iq motif2 and sec7 domain-containing protein 1 isoform a Phyre2 PDB 3m8j chain A Region: 74 - 85 Aligned: 12 Modelled: 12 Confidence: 16.2% Identity: 33% PDB header:transcription Chain: A: PDB Molecule:focb protein; PDBTitle: crystal structure of e.coli focb at 1.4 a resolution Phyre2 PDB 1a87 chain A Region: 23 - 66 Aligned: 44 Modelled: 44 Confidence: 15.8% Identity: 25% PDB header:bacteriocin Chain: A: PDB Molecule:colicin n; PDBTitle: colicin n Phyre2 PDB 1a87 chain A Region: 23 - 66 Aligned: 44 Modelled: 44 Confidence: 15.8% Identity: 25% Fold: Toxins' membrane translocation domains Superfamily: Colicin Family: Colicin Phyre2 PDB 3h0d chain B Region: 72 - 108 Aligned: 37 Modelled: 36 Confidence: 14.2% Identity: 22% PDB header:transcription/dna Chain: B: PDB Molecule:ctsr; PDBTitle: crystal structure of ctsr in complex with a 26bp dna duplex Phyre2 PDB 2ahr chain A domain 1 Region: 63 - 91 Aligned: 28 Modelled: 29 Confidence: 13.5% Identity: 21% Fold: 6-phosphogluconate dehydrogenase C-terminal domain-like Superfamily: 6-phosphogluconate dehydrogenase C-terminal domain-like Family: ProC C-terminal domain-like Phyre2 PDB 2izz chain E Region: 63 - 94 Aligned: 31 Modelled: 32 Confidence: 9.7% Identity: 19% PDB header:oxidoreductase Chain: E: PDB Molecule:pyrroline-5-carboxylate reductase 1; PDBTitle: crystal structure of human pyrroline-5-carboxylate2 reductase Phyre2 PDB 1s7c chain A Region: 66 - 83 Aligned: 18 Modelled: 18 Confidence: 9.4% Identity: 22% PDB header:structural genomics, oxidoreductase Chain: A: PDB Molecule:glyceraldehyde 3-phosphate dehydrogenase a; PDBTitle: crystal structure of mes buffer bound form of glyceraldehyde 3-2 phosphate dehydrogenase from escherichia coli Phyre2 PDB 2i5p chain O Region: 66 - 83 Aligned: 18 Modelled: 18 Confidence: 8.9% Identity: 11% PDB header:oxidoreductase Chain: O: PDB Molecule:glyceraldehyde-3-phosphate dehydrogenase 1; PDBTitle: crystal structure of glyceraldehyde-3-phosphate2 dehydrogenase isoform 1 from k. marxianus Phyre2 PDB 3gty chain S Region: 56 - 105 Aligned: 50 Modelled: 50 Confidence: 8.9% Identity: 10% PDB header:chaperone/ribosomal protein Chain: S: PDB Molecule:30s ribosomal protein s7; PDBTitle: promiscuous substrate recognition in folding and assembly activities2 of the trigger factor chaperone Phyre2 PDB 3h9e chain A Region: 66 - 83 Aligned: 18 Modelled: 0 Confidence: 8.4% Identity: 17% PDB header:oxidoreductase Chain: A: PDB Molecule: PDBTitle: crystal structure of human sperm-specific glyceraldehyde-3-phosphate2 dehydrogenase (gapds) complex with nad and phosphate Phyre2 1 c1ciiA_ 2 c3fewX_ 3 d1yqga1 4 d1cola_ 5 d1rh1a2 6 c1rh1A_ 7 d1uxca_ 8 c2ag8A_ 9 c3mb2J_ 10 c3qwmA_ 11 c3m8jA_ 12 c1a87A_ 13 d1a87a_ 14 c3h0dB_ 15 d2ahra1 16 c2izzE_ 17 c1s7cA_ 18 c2i5pO_ 19 c3gtyS_ 20 c3h9eA_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 c1ciiA_ 66.2 14 PDB header:transmembrane protein Chain: A: PDB Molecule:colicin ia; PDBTitle: colicin ia 2 c3fewX_ 59.7 17 PDB header:immune system Chain: X: PDB Molecule:colicin s4; PDBTitle: structure and function of colicin s4, a colicin with a2 duplicated receptor binding domain 3 d1yqga1 51.0 12 Fold:6-phosphogluconate dehydrogenase C-terminal domain-like Superfamily:6-phosphogluconate dehydrogenase C-terminal domain-like Family:ProC C-terminal domain-like 4 d1cola_ 45.0 17 Fold:Toxins' membrane translocation domains Superfamily:Colicin Family:Colicin 5 d1rh1a2 44.9 10 Fold:Toxins' membrane translocation domains Superfamily:Colicin Family:Colicin 6 c1rh1A_ 28.1 10 PDB header:antibiotic Chain: A: PDB Molecule:colicin b; PDBTitle: crystal structure of the cytotoxic bacterial protein2 colicin b at 2.5 a resolution 7 d1uxca_ 27.5 18 Fold:lambda repressor-like DNA-binding domains Superfamily:lambda repressor-like DNA-binding domains Family:GalR/LacI-like bacterial regulator 8 c2ag8A_ 21.6 12 PDB header:oxidoreductase Chain: A: PDB Molecule:pyrroline-5-carboxylate reductase; PDBTitle: nadp complex of pyrroline-5-carboxylate reductase from neisseria2 meningitidis 9 c3mb2J_ 20.4 26 PDB header:isomerase Chain: J: PDB Molecule:4-oxalocrotonate tautomerase family enzyme - beta subunit; PDBTitle: kinetic and structural characterization of a heterohexamer 4-2 oxalocrotonate tautomerase from chloroflexus aurantiacus j-10-fl:3 implications for functional and structural diversity in the4 tautomerase superfamily 10 c3qwmA_ 17.5 20 PDB header:signaling protein Chain: A: PDB Molecule:iq motif and sec7 domain-containing protein 1; PDBTitle: crystal structure of gep100, the plextrin homology domain of iq motif2 and sec7 domain-containing protein 1 isoform a 11 c3m8jA_ 16.2 33 PDB header:transcription Chain: A: PDB Molecule:focb protein; PDBTitle: crystal structure of e.coli focb at 1.4 a resolution 12 c1a87A_ 15.8 25 PDB header:bacteriocin Chain: A: PDB Molecule:colicin n; PDBTitle: colicin n 13 d1a87a_ 15.8 25 Fold:Toxins' membrane translocation domains Superfamily:Colicin Family:Colicin 14 c3h0dB_ 14.2 22 PDB header:transcription/dna Chain: B: PDB Molecule:ctsr; PDBTitle: crystal structure of ctsr in complex with a 26bp dna duplex 15 d2ahra1 13.5 21 Fold:6-phosphogluconate dehydrogenase C-terminal domain-like Superfamily:6-phosphogluconate dehydrogenase C-terminal domain-like Family:ProC C-terminal domain-like 16 c2izzE_ 9.7 19 PDB header:oxidoreductase Chain: E: PDB Molecule:pyrroline-5-carboxylate reductase 1; PDBTitle: crystal structure of human pyrroline-5-carboxylate2 reductase 17 c1s7cA_ 9.4 22 PDB header:structural genomics, oxidoreductase Chain: A: PDB Molecule:glyceraldehyde 3-phosphate dehydrogenase a; PDBTitle: crystal structure of mes buffer bound form of glyceraldehyde 3-2 phosphate dehydrogenase from escherichia coli 18 c2i5pO_ 8.9 11 PDB header:oxidoreductase Chain: O: PDB Molecule:glyceraldehyde-3-phosphate dehydrogenase 1; PDBTitle: crystal structure of glyceraldehyde-3-phosphate2 dehydrogenase isoform 1 from k. marxianus 19 c3gtyS_ 8.9 10 PDB header:chaperone/ribosomal protein Chain: S: PDB Molecule:30s ribosomal protein s7; PDBTitle: promiscuous substrate recognition in folding and assembly activities2 of the trigger factor chaperone 20 c3h9eA_ 8.4 17 PDB header:oxidoreductase Chain: A: PDB Molecule: PDBTitle: crystal structure of human sperm-specific glyceraldehyde-3-phosphate2 dehydrogenase (gapds) complex with nad and phosphate 21 c1hdgO_ not modelled 8.3 11 PDB header:oxidoreductase (aldehy(d)-nad(a)) Chain: O: PDB Molecule:holo-d-glyceraldehyde-3-phosphate dehydrogenase; PDBTitle: the crystal structure of holo-glyceraldehyde-3-phosphate dehydrogenase2 from the hyperthermophilic bacterium thermotoga maritima at 2.53 angstroms resolution 22 c3ci9B_ not modelled 8.3 21 PDB header:transcription Chain: B: PDB Molecule:heat shock factor-binding protein 1; PDBTitle: crystal structure of the human hsbp1 23 c3b20R_ not modelled 8.2 9 PDB header:oxidoreductase Chain: R: PDB Molecule:glyceraldehyde 3-phosphate dehydrogenase (nadp+); PDBTitle: crystal structure analysis of dehydrogenase complexed with nad 24 c3hq4R_ not modelled 8.1 11 PDB header:oxidoreductase Chain: R: PDB Molecule:glyceraldehyde-3-phosphate dehydrogenase 1; PDBTitle: crystal structure of c151s mutant of glyceraldehyde-3-phosphate2 dehydrogenase 1 (gapdh1) complexed with nad from staphylococcus3 aureus mrsa252 at 2.2 angstrom resolution 25 c3hjaB_ not modelled 8.0 11 PDB header:oxidoreductase Chain: B: PDB Molecule:glyceraldehyde-3-phosphate dehydrogenase; PDBTitle: crystal structure of glyceraldehyde-3-phosphate2 dehydrogenase from borrelia burgdorferi 26 c1ihxD_ not modelled 7.9 17 PDB header:oxidoreductase Chain: D: PDB Molecule:glyceraldehyde 3-phosphate dehydrogenase; PDBTitle: crystal structure of two d-glyceraldehyde-3-phosphate2 dehydrogenase complexes: a case of asymmetry 27 c2d2iO_ not modelled 7.9 11 PDB header:oxidoreductase Chain: O: PDB Molecule:glyceraldehyde 3-phosphate dehydrogenase; PDBTitle: crystal structure of nadp-dependent glyceraldehyde-3-2 phosphate dehydrogenase from synechococcus sp. complexed3 with nadp+ 28 d1k6ka_ not modelled 7.6 16 Fold:Double Clp-N motif Superfamily:Double Clp-N motif Family:Double Clp-N motif 29 c2x5kO_ not modelled 7.4 6 PDB header:oxidoreductase Chain: O: PDB Molecule:d-erythrose-4-phosphate dehydrogenase; PDBTitle: structure of an active site mutant of the d-erythrose-4-phosphate2 dehydrogenase from e. coli 30 c1qvrB_ not modelled 7.4 18 PDB header:chaperone Chain: B: PDB Molecule:clpb protein; PDBTitle: crystal structure analysis of clpb 31 c2ep7B_ not modelled 7.2 17 PDB header:oxidoreductase Chain: B: PDB Molecule:glyceraldehyde-3-phosphate dehydrogenase; PDBTitle: structural study of project id aq_1065 from aquifex aeolicus vf5 32 d1u94a2 not modelled 7.2 19 Fold:Anti-LPS factor/recA domain Superfamily:RecA protein, C-terminal domain Family:RecA protein, C-terminal domain 33 d2bida_ not modelled 7.1 9 Fold:Toxins' membrane translocation domains Superfamily:Bcl-2 inhibitors of programmed cell death Family:Bcl-2 inhibitors of programmed cell death 34 c2pkrI_ not modelled 7.1 11 PDB header:oxidoreductase Chain: I: PDB Molecule:glyceraldehyde-3-phosphate dehydrogenase aor; PDBTitle: crystal structure of (a+cte)4 chimeric form of2 photosyntetic glyceraldehyde-3-phosphate dehydrogenase,3 complexed with nadp 35 c3docD_ not modelled 7.0 11 PDB header:oxidoreductase Chain: D: PDB Molecule:glyceraldehyde 3-phosphate dehydrogenase; PDBTitle: crystal structure of trka glyceraldehyde-3-phosphate2 dehydrogenase from brucella melitensis 36 c1rm4O_ not modelled 6.9 11 PDB header:oxidoreductase Chain: O: PDB Molecule:glyceraldehyde 3-phosphate dehydrogenase a; PDBTitle: crystal structure of recombinant photosynthetic glyceraldehyde-3-2 phosphate dehydrogenase a4 isoform, complexed with nadp 37 c2fs1A_ not modelled 6.9 15 PDB header:protein binding Chain: A: PDB Molecule:psd-1; PDBTitle: solution structure of psd-1 38 d1xp8a2 not modelled 6.9 20 Fold:Anti-LPS factor/recA domain Superfamily:RecA protein, C-terminal domain Family:RecA protein, C-terminal domain 39 c2b4rQ_ not modelled 6.8 11 PDB header:oxidoreductase Chain: Q: PDB Molecule:glyceraldehyde-3-phosphate dehydrogenase; PDBTitle: crystal structure of glyceraldehyde-3-phosphate dehydrogenase from2 plasmodium falciparum at 2.25 angstrom resolution reveals intriguing3 extra electron density in the active site 40 c1i32D_ not modelled 6.8 13 PDB header:oxidoreductase Chain: D: PDB Molecule:glyceraldehyde 3-phosphate dehydrogenase; PDBTitle: leishmania mexicana glyceraldehyde-3-phosphate2 dehydrogenase in complex with inhibitors 41 c1cerC_ not modelled 6.6 6 PDB header:oxidoreductase (aldehyde(d)-nad(a)) Chain: C: PDB Molecule:holo-d-glyceraldehyde-3-phosphate dehydrogenase; PDBTitle: determinants of enzyme thermostability observed in the2 molecular structure of thermus aquaticus d-glyceraldehyde-3 3-phosphate dehydrogenase at 2.5 angstroms resolution 42 d1mo6a2 not modelled 6.5 16 Fold:Anti-LPS factor/recA domain Superfamily:RecA protein, C-terminal domain Family:RecA protein, C-terminal domain 43 d1ubea2 not modelled 6.4 16 Fold:Anti-LPS factor/recA domain Superfamily:RecA protein, C-terminal domain Family:RecA protein, C-terminal domain 44 c3cieC_ not modelled 6.3 17 PDB header:oxidoreductase Chain: C: PDB Molecule:glyceraldehyde-3-phosphate dehydrogenase; PDBTitle: crystal structure of glyceraldehyde 3-phosphate2 dehydrogenase from cryptosporidium parvum 45 c1obfO_ not modelled 6.2 11 PDB header:glycolytic pathway Chain: O: PDB Molecule:glyceraldehyde 3-phosphate dehydrogenase; PDBTitle: the crystal structure of glyceraldehyde 3-phosphate2 dehydrogenase from alcaligenes xylosoxidans at 1.73 resolution. 46 d2c42a4 not modelled 6.1 18 Fold:Pyruvate-ferredoxin oxidoreductase, PFOR, domain III Superfamily:Pyruvate-ferredoxin oxidoreductase, PFOR, domain III Family:Pyruvate-ferredoxin oxidoreductase, PFOR, domain III 47 d1gjsa_ not modelled 6.1 23 Fold:immunoglobulin/albumin-binding domain-like Superfamily:Bacterial immunoglobulin/albumin-binding domains Family:GA module, an albumin-binding domain 48 c2i88A_ not modelled 6.1 15 PDB header:membrane protein Chain: A: PDB Molecule:colicin-e1; PDBTitle: crystal structure of the channel-forming domain of colicin2 e1 49 d1v9va1 not modelled 6.1 17 Fold:Bromodomain-like Superfamily:MAST3 pre-PK domain-like Family:MAST3 pre-PK domain-like 50 c3sthA_ not modelled 5.6 17 PDB header:oxidoreductase Chain: A: PDB Molecule:glyceraldehyde-3-phosphate dehydrogenase; PDBTitle: crystal structure of glyceraldehyde-3-phosphate dehydrogenase from2 toxoplasma gondii 51 c2y69Z_ not modelled 5.5 17 PDB header:electron transport Chain: Z: PDB Molecule:cytochrome c oxidase polypeptide 8h; PDBTitle: bovine heart cytochrome c oxidase re-refined with molecular2 oxygen 52 c1g92A_ not modelled 5.4 67 PDB header:toxin Chain: A: PDB Molecule:poneratoxin; PDBTitle: solution structure of poneratoxin 53 c2po3B_ not modelled 5.3 22 PDB header:transferase Chain: B: PDB Molecule:4-dehydrase; PDBTitle: crystal structure analysis of desi in the presence of its2 tdp-sugar product 54 c2gd1P_ not modelled 5.1 11 PDB header:oxidoreductase(aldehyde(d)-nad(a)) Chain: P: PDB Molecule:apo-d-glyceraldehyde-3-phosphate dehydrogenase; PDBTitle: coenzyme-induced conformational changes in glyceraldehyde-3-2 phosphate dehydrogenase from bacillus stearothermophillus 55 d1hula_ not modelled 5.1 17 Fold:4-helical cytokines Superfamily:4-helical cytokines Family:Short-chain cytokines

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

Transmembrane helix prediction

Transmembrane helices have been predicted in your sequence to adopt the topology shown below

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0