Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	D	S	F	P	A	I	E	I	D	K	V	K	A	W	D	F	R	L	A	N	I	N	T	S	E	C	L	N	V	A	Y	G	V	D	A	N	Y	L	D	G	V	G	V	S	I	T	S	I	V	L	N	N	R	H	I	N	L	D	F
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?		?	?	?	?	?		?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		Y	I	I	A	D	V	Y	N	D	G	F	F	Q	K	I	A	K	L	A	E	Q	N	Q	L	R	I	T	L	Y	R	I	N	T	D	K	L	Q	C	L	P	C	T	Q	V	W	S	R	A	M	Y	F	R	L	F	A	F	Q	L	L	G
Secondary structure
SS confidence
Disorder																																							?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		L	T	L	D	R	L	L	Y	L	D	A	D	V	V	C	K	G	D	I	S	Q	L	L	H	L	G	L	N	G	A	V	A	A	V	V	K	D	V	E	P	M	Q	E	K	A	V	S	R	L	S	D	P	E	L	L	G	Q	Y	F	N
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		S	G	V	V	Y	L	D	L	K	K	W	A	D	A	K	L	T	E	K	A	L	S	I	L	M	S	K	D	N	V	Y	K	Y	P	D	Q	D	V	M	N	V	L	L	K	G	M	T	L	F	L	P	R	E	Y	N	T	I	Y	T	I
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		K	S	E	L	K	D	K	T	H	Q	N	Y	K	K	L	I	T	E	S	T	L	L	I	H	Y	T	G	A	T	K	P	W	H	K	W	A	I	Y	P	S	V	K	Y	Y	K	I	A	L	E	N	S	P	W	K	D	D	S	P	R	D
Secondary structure
SS confidence
Disorder				?																																																				?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.
Sequence		A	K	S	I	I	E	F	K	K	R	Y	K	H	L	L	V	Q	H	H	Y	I	S	G	I	I	A	G	V	C	Y	L	C	R	K	Y	Y	R	K
Secondary structure
SS confidence
Disorder		?		?														?	?	?	?															?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 1ga8 chain A Region: 29 - 296 Aligned: 267 Modelled: 268 Confidence: 100.0% Identity: 19% Fold: Nucleotide-diphospho-sugar transferases Superfamily: Nucleotide-diphospho-sugar transferases Family: Galactosyltransferase LgtC Phyre2 PDB 1ga8 chain A Region: 29 - 296 Aligned: 267 Modelled: 268 Confidence: 100.0% Identity: 19% PDB header:transferase Chain: A: PDB Molecule:galactosyl transferase lgtc; PDBTitle: crystal structure of galacosyltransferase lgtc in complex2 with donor and acceptor sugar analogs. Phyre2 PDB 3tzt chain B Region: 30 - 293 Aligned: 224 Modelled: 232 Confidence: 100.0% Identity: 24% PDB header:transferase Chain: B: PDB Molecule:glycosyl transferase family 8; PDBTitle: the structure of a protein in glycosyl transferase family 8 from2 anaerococcus prevotii. Phyre2 PDB 1zcy chain A Region: 30 - 289 Aligned: 230 Modelled: 245 Confidence: 100.0% Identity: 17% PDB header:transferase Chain: A: PDB Molecule:glycogenin-1; PDBTitle: apo form of a mutant of glycogenin in which asp159 is replaced by ser Phyre2 PDB 1ll2 chain A Region: 25 - 292 Aligned: 238 Modelled: 250 Confidence: 100.0% Identity: 17% Fold: Nucleotide-diphospho-sugar transferases Superfamily: Nucleotide-diphospho-sugar transferases Family: Glycogenin Phyre2 PDB 1zct chain B Region: 25 - 291 Aligned: 237 Modelled: 252 Confidence: 100.0% Identity: 16% PDB header:transferase Chain: B: PDB Molecule:glycogenin-1; PDBTitle: structure of glycogenin truncated at residue 270 in a2 complex with udp Phyre2 PDB 1xhb chain A Region: 30 - 156 Aligned: 114 Modelled: 127 Confidence: 95.3% Identity: 13% PDB header:transferase Chain: A: PDB Molecule:polypeptide n-acetylgalactosaminyltransferase 1; PDBTitle: the crystal structure of udp-galnac: polypeptide alpha-n-2 acetylgalactosaminyltransferase-t1 Phyre2 PDB 1o7q chain A Region: 27 - 236 Aligned: 193 Modelled: 210 Confidence: 92.7% Identity: 15% Fold: Nucleotide-diphospho-sugar transferases Superfamily: Nucleotide-diphospho-sugar transferases Family: alpha-1,3-galactosyltransferase-like Phyre2 PDB 2d7i chain A Region: 30 - 156 Aligned: 113 Modelled: 127 Confidence: 88.9% Identity: 10% PDB header:transferase Chain: A: PDB Molecule:polypeptide n-acetylgalactosaminyltransferase 10; PDBTitle: crsytal structure of pp-galnac-t10 with udp, galnac and mn2+ Phyre2 PDB 2ffu chain A Region: 30 - 156 Aligned: 110 Modelled: 123 Confidence: 85.2% Identity: 15% PDB header:transferase Chain: A: PDB Molecule:polypeptide n-acetylgalactosaminyltransferase 2; PDBTitle: crystal structure of human ppgalnact-2 complexed with udp2 and ea2 Phyre2 PDB 1fo8 chain A Region: 30 - 144 Aligned: 112 Modelled: 115 Confidence: 70.8% Identity: 11% Fold: Nucleotide-diphospho-sugar transferases Superfamily: Nucleotide-diphospho-sugar transferases Family: N-acetylglucosaminyltransferase I Phyre2 PDB 2py5 chain A domain 2 Region: 107 - 136 Aligned: 30 Modelled: 30 Confidence: 67.9% Identity: 20% Fold: DNA/RNA polymerases Superfamily: DNA/RNA polymerases Family: DNA polymerase I Phyre2 PDB 2ex3 chain I Region: 107 - 140 Aligned: 34 Modelled: 34 Confidence: 57.2% Identity: 18% PDB header:transferase/replication Chain: I: PDB Molecule:dna polymerase; PDBTitle: bacteriophage phi29 dna polymerase bound to terminal protein Phyre2 PDB 1lzi chain A Region: 27 - 236 Aligned: 179 Modelled: 188 Confidence: 45.3% Identity: 12% Fold: Nucleotide-diphospho-sugar transferases Superfamily: Nucleotide-diphospho-sugar transferases Family: alpha-1,3-galactosyltransferase-like Phyre2 PDB 1w7p chain D domain 2 Region: 162 - 209 Aligned: 46 Modelled: 48 Confidence: 42.8% Identity: 15% Fold: DNA/RNA-binding 3-helical bundle Superfamily: "Winged helix" DNA-binding domain Family: Vacuolar sorting protein domain Phyre2 PDB 2npi chain B Region: 102 - 133 Aligned: 32 Modelled: 32 Confidence: 30.2% Identity: 16% PDB header:transcription Chain: B: PDB Molecule:protein clp1; PDBTitle: clp1-atp-pcf11 complex Phyre2 PDB 2z86 chain D Region: 20 - 138 Aligned: 104 Modelled: 119 Confidence: 26.5% Identity: 6% PDB header:transferase Chain: D: PDB Molecule:chondroitin synthase; PDBTitle: crystal structure of chondroitin polymerase from2 escherichia coli strain k4 (k4cp) complexed with udp-glcua3 and udp Phyre2 PDB 2p73 chain A Region: 115 - 267 Aligned: 124 Modelled: 139 Confidence: 24.2% Identity: 19% PDB header:transferase Chain: A: PDB Molecule:putative glycosyltransferase (mannosyltransferase) involved PDBTitle: crystal structure of a glycosyltransferase involved in the2 glycosylation of the major capsid of pbcv-1 Phyre2 PDB 1qg8 chain A Region: 42 - 138 Aligned: 89 Modelled: 97 Confidence: 23.4% Identity: 20% Fold: Nucleotide-diphospho-sugar transferases Superfamily: Nucleotide-diphospho-sugar transferases Family: Spore coat polysaccharide biosynthesis protein SpsA Phyre2 PDB 3lma chain C Region: 38 - 129 Aligned: 91 Modelled: 91 Confidence: 23.3% Identity: 12% PDB header:membrane protein Chain: C: PDB Molecule:stage v sporulation protein ad (spovad); PDBTitle: crystal structure of the stage v sporulation protein ad2 (spovad) from bacillus licheniformis. northeast structural3 genomics consortium target bir6. Phyre2 1 d1ga8a_ 2 c1ga8A_ 3 c3tztB_ 4 c1zcyA_ 5 d1ll2a_ 6 c1zctB_ 7 c1xhbA_ 8 d1o7qa_ 9 c2d7iA_ 10 c2ffuA_ 11 d1fo8a_ 12 d2py5a2 13 c2ex3I_ 14 d1lzia_ 15 d1w7pd2 16 c2npiB_ 17 c2z86D_ 18 c2p73A_ 19 d1qg8a_ 20 c3lmaC_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 d1ga8a_ 100.0 19 Fold:Nucleotide-diphospho-sugar transferases Superfamily:Nucleotide-diphospho-sugar transferases Family:Galactosyltransferase LgtC 2 c1ga8A_ 100.0 19 PDB header:transferase Chain: A: PDB Molecule:galactosyl transferase lgtc; PDBTitle: crystal structure of galacosyltransferase lgtc in complex2 with donor and acceptor sugar analogs. 3 c3tztB_ 100.0 24 PDB header:transferase Chain: B: PDB Molecule:glycosyl transferase family 8; PDBTitle: the structure of a protein in glycosyl transferase family 8 from2 anaerococcus prevotii. 4 c1zcyA_ 100.0 17 PDB header:transferase Chain: A: PDB Molecule:glycogenin-1; PDBTitle: apo form of a mutant of glycogenin in which asp159 is replaced by ser 5 d1ll2a_ 100.0 17 Fold:Nucleotide-diphospho-sugar transferases Superfamily:Nucleotide-diphospho-sugar transferases Family:Glycogenin 6 c1zctB_ 100.0 16 PDB header:transferase Chain: B: PDB Molecule:glycogenin-1; PDBTitle: structure of glycogenin truncated at residue 270 in a2 complex with udp 7 c1xhbA_ 95.3 13 PDB header:transferase Chain: A: PDB Molecule:polypeptide n-acetylgalactosaminyltransferase 1; PDBTitle: the crystal structure of udp-galnac: polypeptide alpha-n-2 acetylgalactosaminyltransferase-t1 8 d1o7qa_ 92.7 15 Fold:Nucleotide-diphospho-sugar transferases Superfamily:Nucleotide-diphospho-sugar transferases Family:alpha-1,3-galactosyltransferase-like 9 c2d7iA_ 88.9 10 PDB header:transferase Chain: A: PDB Molecule:polypeptide n-acetylgalactosaminyltransferase 10; PDBTitle: crsytal structure of pp-galnac-t10 with udp, galnac and mn2+ 10 c2ffuA_ 85.2 15 PDB header:transferase Chain: A: PDB Molecule:polypeptide n-acetylgalactosaminyltransferase 2; PDBTitle: crystal structure of human ppgalnact-2 complexed with udp2 and ea2 11 d1fo8a_ 70.8 11 Fold:Nucleotide-diphospho-sugar transferases Superfamily:Nucleotide-diphospho-sugar transferases Family:N-acetylglucosaminyltransferase I 12 d2py5a2 67.9 20 Fold:DNA/RNA polymerases Superfamily:DNA/RNA polymerases Family:DNA polymerase I 13 c2ex3I_ 57.2 18 PDB header:transferase/replication Chain: I: PDB Molecule:dna polymerase; PDBTitle: bacteriophage phi29 dna polymerase bound to terminal protein 14 d1lzia_ 45.3 12 Fold:Nucleotide-diphospho-sugar transferases Superfamily:Nucleotide-diphospho-sugar transferases Family:alpha-1,3-galactosyltransferase-like 15 d1w7pd2 42.8 15 Fold:DNA/RNA-binding 3-helical bundle Superfamily:"Winged helix" DNA-binding domain Family:Vacuolar sorting protein domain 16 c2npiB_ 30.2 16 PDB header:transcription Chain: B: PDB Molecule:protein clp1; PDBTitle: clp1-atp-pcf11 complex 17 c2z86D_ 26.5 6 PDB header:transferase Chain: D: PDB Molecule:chondroitin synthase; PDBTitle: crystal structure of chondroitin polymerase from2 escherichia coli strain k4 (k4cp) complexed with udp-glcua3 and udp 18 c2p73A_ 24.2 19 PDB header:transferase Chain: A: PDB Molecule:putative glycosyltransferase (mannosyltransferase) involved PDBTitle: crystal structure of a glycosyltransferase involved in the2 glycosylation of the major capsid of pbcv-1 19 d1qg8a_ 23.4 20 Fold:Nucleotide-diphospho-sugar transferases Superfamily:Nucleotide-diphospho-sugar transferases Family:Spore coat polysaccharide biosynthesis protein SpsA 20 c3lmaC_ 23.3 12 PDB header:membrane protein Chain: C: PDB Molecule:stage v sporulation protein ad (spovad); PDBTitle: crystal structure of the stage v sporulation protein ad2 (spovad) from bacillus licheniformis. northeast structural3 genomics consortium target bir6. 21 d1ibia1 not modelled 22.6 64 Fold:Glucocorticoid receptor-like (DNA-binding domain) Superfamily:Glucocorticoid receptor-like (DNA-binding domain) Family:LIM domain 22 d1s4na_ not modelled 18.4 16 Fold:Nucleotide-diphospho-sugar transferases Superfamily:Nucleotide-diphospho-sugar transferases Family:Glycolipid 2-alpha-mannosyltransferase 23 c2w3zA_ not modelled 18.0 10 PDB header:hydrolase Chain: A: PDB Molecule:putative deacetylase; PDBTitle: structure of a streptococcus mutans ce4 esterase 24 d1acoa2 not modelled 16.7 14 Fold:Aconitase iron-sulfur domain Superfamily:Aconitase iron-sulfur domain Family:Aconitase iron-sulfur domain 25 c3ew8A_ not modelled 15.5 30 PDB header:hydrolase Chain: A: PDB Molecule:histone deacetylase 8; PDBTitle: crystal structure analysis of human hdac8 d101l variant 26 d2c71a1 not modelled 12.6 14 Fold:7-stranded beta/alpha barrel Superfamily:Glycoside hydrolase/deacetylase Family:NodB-like polysaccharide deacetylase 27 c3maxB_ not modelled 12.5 35 PDB header:hydrolase Chain: B: PDB Molecule:histone deacetylase 2; PDBTitle: crystal structure of human hdac2 complexed with an n-(2-aminophenyl)2 benzamide 28 c2ktvA_ not modelled 11.7 18 PDB header:translation Chain: A: PDB Molecule:eukaryotic peptide chain release factor subunit 1; PDBTitle: human erf1 c-domain, "open" conformer 29 c4a69A_ not modelled 11.6 30 PDB header:transcription Chain: A: PDB Molecule:histone deacetylase 3,; PDBTitle: structure of hdac3 bound to corepressor and inositol tetraphosphate 30 d1xhba2 not modelled 11.3 15 Fold:Nucleotide-diphospho-sugar transferases Superfamily:Nucleotide-diphospho-sugar transferases Family:Polypeptide N-acetylgalactosaminyltransferase 1, N-terminal domain 31 c3ckvA_ not modelled 11.0 12 PDB header:unknown function Chain: A: PDB Molecule:putative uncharacterized protein; PDBTitle: crystal structure of a mycobacterial protein 32 c3m9qA_ not modelled 10.7 20 PDB header:dna binding protein Chain: A: PDB Molecule:protein male-specific lethal-3; PDBTitle: drosophila msl3 chromodomain 33 c1hf2A_ not modelled 10.6 17 PDB header:cell division protein Chain: A: PDB Molecule:septum site-determining protein minc; PDBTitle: crystal structure of the bacterial cell-division inhibitor2 minc from t. maritima 34 d1u5tb1 not modelled 10.3 13 Fold:DNA/RNA-binding 3-helical bundle Superfamily:"Winged helix" DNA-binding domain Family:Vacuolar sorting protein domain 35 c3e20C_ not modelled 10.1 24 PDB header:translation Chain: C: PDB Molecule:eukaryotic peptide chain release factor subunit 1; PDBTitle: crystal structure of s.pombe erf1/erf3 complex 36 c3m9pA_ not modelled 9.5 40 PDB header:dna binding protein/dna Chain: A: PDB Molecule:male-specific lethal 3 homolog; PDBTitle: human msl3 chromodomain bound to dna and h4k20me1 peptide 37 d1hf2a1 not modelled 8.9 17 Fold:Single-stranded right-handed beta-helix Superfamily:Cell-division inhibitor MinC, C-terminal domain Family:Cell-division inhibitor MinC, C-terminal domain 38 d1t64a_ not modelled 6.9 18 Fold:Arginase/deacetylase Superfamily:Arginase/deacetylase Family:Histone deacetylase, HDAC 39 d1dt9a2 not modelled 6.6 18 Fold:Bacillus chorismate mutase-like Superfamily:L30e-like Family:ERF1/Dom34 C-terminal domain-like 40 c1w17A_ not modelled 6.4 11 PDB header:hydrolase Chain: A: PDB Molecule:probable polysaccharide deacetylase pdaa; PDBTitle: structure of bacillus subtilis pdaa, a family 42 carbohydrate esterase. 41 d1c3pa_ not modelled 6.1 29 Fold:Arginase/deacetylase Superfamily:Arginase/deacetylase Family:Histone deacetylase, HDAC 42 c3menC_ not modelled 5.9 35 PDB header:hydrolase Chain: C: PDB Molecule:acetylpolyamine aminohydrolase; PDBTitle: crystal structure of acetylpolyamine aminohydrolase from burkholderia2 pseudomallei, iodide soak 43 c2zmeB_ not modelled 5.8 26 PDB header:protein transport Chain: B: PDB Molecule:vacuolar protein-sorting-associated protein 36; PDBTitle: integrated structural and functional model of the human escrt-ii2 complex 44 d1ctla1 not modelled 5.8 55 Fold:Glucocorticoid receptor-like (DNA-binding domain) Superfamily:Glucocorticoid receptor-like (DNA-binding domain) Family:LIM domain 45 d2f5ka1 not modelled 5.6 47 Fold:SH3-like barrel Superfamily:Chromo domain-like Family:Chromo barrel domain 46 d2g3ra2 not modelled 5.5 56 Fold:SH3-like barrel Superfamily:Tudor/PWWP/MBT Family:Tudor domain 47 c3bcvA_ not modelled 5.4 12 PDB header:transferase Chain: A: PDB Molecule:putative glycosyltransferase protein; PDBTitle: crystal structure of a putative glycosyltransferase from bacteroides2 fragilis 48 c3ir9A_ not modelled 5.4 16 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:peptide chain release factor subunit 1; PDBTitle: c-terminal domain of peptide chain release factor from2 methanosarcina mazei. 49 c1omxB_ not modelled 5.3 11 PDB header:transferase Chain: B: PDB Molecule:alpha-1,4-n-acetylhexosaminyltransferase extl2; PDBTitle: crystal structure of mouse alpha-1,4-n-2 acetylhexosaminyltransferase (extl2)

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

Transmembrane helix prediction

Transmembrane helices have been predicted in your sequence to adopt the topology shown below

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0