Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	K	K	I	N	A	I	I	L	L	S	S	L	T	S	A	S	V	F	A	G	A	Y	V	E	N	R	E	A	Y	N	L	A	S	D	Q	G	E	V	M	L	R	V	G	Y	N	F	D	M	G	A	G	I	M	L	T	N	T	Y	N
Secondary structure
SS confidence
Disorder		?	?	?	?											?	?	?	?	?												?	?	?	?	?	?	?																						?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		F	Q	R	E	D	E	L	K	H	G	Y	N	E	I	E	G	W	Y	P	L	F	K	P	T	D	K	L	T	I	Q	P	G	G	L	I	N	D	K	S	I	G	S	G	G	A	V	Y	L	D	V	N	Y	K	F	V	P	W	F	N	L
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?	?	?	?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		T	V	R	N	R	Y	N	H	N	N	Y	S	S	T	D	L	S	G	E	L	D	N	N	D	T	Y	E	I	G	T	Y	W	N	F	K	I	T	D	K	F	S	Y	T	F	E	P	H	Y	F	M	R	V	N	D	F	N	S	S	N	G
Secondary structure
SS confidence
Disorder											?	?	?	?	?	?	?	?	?	?	?	?	?	?																																		?		?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230
Sequence		K	D	H	H	W	E	I	T	N	T	F	R	Y	R	I	N	E	H	W	L	P	Y	F	E	L	R	W	L	D	R	N	V	E	P	Y	H	R	E	Q	N	Q	I	R	I	G	T	K	Y	F	F
Secondary structure
SS confidence
Disorder		?																												?	?	?	?	?	?	?	?	?	?											?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 2wjq chain A Region: 22 - 229 Aligned: 201 Modelled: 208 Confidence: 100.0% Identity: 24% PDB header:transport protein Chain: A: PDB Molecule:probable n-acetylneuraminic acid outer membrane channel PDBTitle: nanc porin structure in hexagonal crystal form. Phyre2 PDB 2iwv chain D Region: 51 - 230 Aligned: 175 Modelled: 180 Confidence: 98.3% Identity: 16% PDB header:ion channel Chain: D: PDB Molecule:outer membrane protein g; PDBTitle: structure of the monomeric outer membrane porin ompg in the2 open and closed conformation Phyre2 PDB 2f1t chain B Region: 86 - 230 Aligned: 144 Modelled: 145 Confidence: 97.8% Identity: 13% PDB header:membrane protein Chain: B: PDB Molecule:outer membrane protein w; PDBTitle: outer membrane protein ompw Phyre2 PDB 1g90 chain A Region: 48 - 230 Aligned: 158 Modelled: 158 Confidence: 97.7% Identity: 9% Fold: Transmembrane beta-barrels Superfamily: OMPA-like Family: Outer membrane protein Phyre2 PDB 3aeh chain B Region: 31 - 230 Aligned: 153 Modelled: 165 Confidence: 97.4% Identity: 16% PDB header:hydrolase Chain: B: PDB Molecule:hemoglobin-binding protease hbp autotransporter; PDBTitle: integral membrane domain of autotransporter hbp Phyre2 PDB 2jmm chain A Region: 84 - 230 Aligned: 139 Modelled: 147 Confidence: 97.3% Identity: 12% PDB header:membrane protein Chain: A: PDB Molecule:outer membrane protein a; PDBTitle: nmr solution structure of a minimal transmembrane beta-2 barrel platform protein Phyre2 PDB 1qjp chain A Region: 85 - 230 Aligned: 127 Modelled: 146 Confidence: 97.0% Identity: 13% Fold: Transmembrane beta-barrels Superfamily: OMPA-like Family: Outer membrane protein Phyre2 PDB 1p4t chain A Region: 87 - 230 Aligned: 143 Modelled: 144 Confidence: 97.0% Identity: 7% Fold: Transmembrane beta-barrels Superfamily: OMPA-like Family: Outer membrane protein Phyre2 PDB 3a2r chain X Region: 107 - 230 Aligned: 121 Modelled: 124 Confidence: 97.0% Identity: 8% PDB header:membrane protein Chain: X: PDB Molecule:outer membrane protein ii; PDBTitle: crystal structure of outer membrane protein porb from neisseria2 meningitidis Phyre2 PDB 3qra chain A Region: 83 - 230 Aligned: 142 Modelled: 148 Confidence: 96.8% Identity: 12% PDB header:cell invasion Chain: A: PDB Molecule:attachment invasion locus protein; PDBTitle: the crystal structure of ail, the attachment invasion locus protein of2 yersinia pestis Phyre2 PDB 2k0l chain A Region: 98 - 230 Aligned: 133 Modelled: 133 Confidence: 96.5% Identity: 8% PDB header:membrane protein Chain: A: PDB Molecule:outer membrane protein a; PDBTitle: nmr structure of the transmembrane domain of the outer2 membrane protein a from klebsiella pneumoniae in dhpc3 micelles. Phyre2 PDB 3qq2 chain C Region: 19 - 230 Aligned: 160 Modelled: 164 Confidence: 96.4% Identity: 17% PDB header:membrane protein/protein transport Chain: C: PDB Molecule:brka autotransporter; PDBTitle: crystal structure of the beta domain of the bordetella autotransporter2 brka Phyre2 PDB 3slj chain A Region: 31 - 230 Aligned: 166 Modelled: 170 Confidence: 96.4% Identity: 19% PDB header:protein transport Chain: A: PDB Molecule:serine protease espp; PDBTitle: pre-cleavage structure of the autotransporter espp - n1023a mutant Phyre2 PDB 3nb3 chain C Region: 23 - 126 Aligned: 103 Modelled: 104 Confidence: 95.9% Identity: 13% PDB header:virus Chain: C: PDB Molecule:outer membrane protein a; PDBTitle: the host outer membrane proteins ompa and ompc are packed at specific2 sites in the shigella phage sf6 virion as structural components Phyre2 PDB 2qom chain B Region: 41 - 230 Aligned: 158 Modelled: 162 Confidence: 95.7% Identity: 20% PDB header:hydrolase Chain: B: PDB Molecule:serine protease espp; PDBTitle: the crystal structure of the e.coli espp autotransporter beta-domain. Phyre2 PDB 2fgq chain X domain 1 Region: 49 - 230 Aligned: 168 Modelled: 175 Confidence: 95.3% Identity: 12% Fold: Transmembrane beta-barrels Superfamily: Porins Family: Porin Phyre2 PDB 1t16 chain A Region: 39 - 196 Aligned: 157 Modelled: 158 Confidence: 94.8% Identity: 11% Fold: Transmembrane beta-barrels Superfamily: Porins Family: Outer membrane protein transport protein Phyre2 PDB 1qj8 chain A Region: 88 - 230 Aligned: 139 Modelled: 143 Confidence: 93.0% Identity: 17% Fold: Transmembrane beta-barrels Superfamily: OMPA-like Family: Outer membrane protein Phyre2 PDB 2hdi chain A Region: 90 - 230 Aligned: 132 Modelled: 141 Confidence: 91.9% Identity: 11% PDB header:protein transport,antimicrobial protein Chain: A: PDB Molecule:colicin i receptor; PDBTitle: crystal structure of the colicin i receptor cir from e.coli in complex2 with receptor binding domain of colicin ia. Phyre2 PDB 1pho chain A Region: 47 - 230 Aligned: 178 Modelled: 184 Confidence: 89.9% Identity: 11% Fold: Transmembrane beta-barrels Superfamily: Porins Family: Porin Phyre2 1 c2wjqA_ 2 c2iwvD_ 3 c2f1tB_ 4 d1g90a_ 5 c3aehB_ 6 c2jmmA_ 7 d1qjpa_ 8 d1p4ta_ 9 c3a2rX_ 10 c3qraA_ 11 c2k0lA_ 12 c3qq2C_ 13 c3sljA_ 14 c3nb3C_ 15 c2qomB_ 16 d2fgqx1 17 d1t16a_ 18 d1qj8a_ 19 c2hdiA_ 20 d1phoa_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 c2wjqA_ 100.0 24 PDB header:transport protein Chain: A: PDB Molecule:probable n-acetylneuraminic acid outer membrane channel PDBTitle: nanc porin structure in hexagonal crystal form. 2 c2iwvD_ 98.3 16 PDB header:ion channel Chain: D: PDB Molecule:outer membrane protein g; PDBTitle: structure of the monomeric outer membrane porin ompg in the2 open and closed conformation 3 c2f1tB_ 97.8 13 PDB header:membrane protein Chain: B: PDB Molecule:outer membrane protein w; PDBTitle: outer membrane protein ompw 4 d1g90a_ 97.7 9 Fold:Transmembrane beta-barrels Superfamily:OMPA-like Family:Outer membrane protein 5 c3aehB_ 97.4 16 PDB header:hydrolase Chain: B: PDB Molecule:hemoglobin-binding protease hbp autotransporter; PDBTitle: integral membrane domain of autotransporter hbp 6 c2jmmA_ 97.3 12 PDB header:membrane protein Chain: A: PDB Molecule:outer membrane protein a; PDBTitle: nmr solution structure of a minimal transmembrane beta-2 barrel platform protein 7 d1qjpa_ 97.0 13 Fold:Transmembrane beta-barrels Superfamily:OMPA-like Family:Outer membrane protein 8 d1p4ta_ 97.0 7 Fold:Transmembrane beta-barrels Superfamily:OMPA-like Family:Outer membrane protein 9 c3a2rX_ 97.0 8 PDB header:membrane protein Chain: X: PDB Molecule:outer membrane protein ii; PDBTitle: crystal structure of outer membrane protein porb from neisseria2 meningitidis 10 c3qraA_ 96.8 12 PDB header:cell invasion Chain: A: PDB Molecule:attachment invasion locus protein; PDBTitle: the crystal structure of ail, the attachment invasion locus protein of2 yersinia pestis 11 c2k0lA_ 96.5 8 PDB header:membrane protein Chain: A: PDB Molecule:outer membrane protein a; PDBTitle: nmr structure of the transmembrane domain of the outer2 membrane protein a from klebsiella pneumoniae in dhpc3 micelles. 12 c3qq2C_ 96.4 17 PDB header:membrane protein/protein transport Chain: C: PDB Molecule:brka autotransporter; PDBTitle: crystal structure of the beta domain of the bordetella autotransporter2 brka 13 c3sljA_ 96.4 19 PDB header:protein transport Chain: A: PDB Molecule:serine protease espp; PDBTitle: pre-cleavage structure of the autotransporter espp - n1023a mutant 14 c3nb3C_ 95.9 13 PDB header:virus Chain: C: PDB Molecule:outer membrane protein a; PDBTitle: the host outer membrane proteins ompa and ompc are packed at specific2 sites in the shigella phage sf6 virion as structural components 15 c2qomB_ 95.7 20 PDB header:hydrolase Chain: B: PDB Molecule:serine protease espp; PDBTitle: the crystal structure of the e.coli espp autotransporter beta-domain. 16 d2fgqx1 95.3 12 Fold:Transmembrane beta-barrels Superfamily:Porins Family:Porin 17 d1t16a_ 94.8 11 Fold:Transmembrane beta-barrels Superfamily:Porins Family:Outer membrane protein transport protein 18 d1qj8a_ 93.0 17 Fold:Transmembrane beta-barrels Superfamily:OMPA-like Family:Outer membrane protein 19 c2hdiA_ 91.9 11 PDB header:protein transport,antimicrobial protein Chain: A: PDB Molecule:colicin i receptor; PDBTitle: crystal structure of the colicin i receptor cir from e.coli in complex2 with receptor binding domain of colicin ia. 20 d1phoa_ 89.9 11 Fold:Transmembrane beta-barrels Superfamily:Porins Family:Porin 21 d1osma_ not modelled 89.0 11 Fold:Transmembrane beta-barrels Superfamily:Porins Family:Porin 22 c3dwoX_ not modelled 88.7 18 PDB header:membrane protein Chain: X: PDB Molecule:probable outer membrane protein; PDBTitle: crystal structure of a pseudomonas aeruginosa fadl homologue 23 c2ervA_ not modelled 85.6 19 PDB header:membrane protein Chain: A: PDB Molecule:hypothetical protein paer03002360; PDBTitle: crystal structure of the outer membrane enzyme pagl 24 c2x27X_ not modelled 83.5 16 PDB header:membrane protein Chain: X: PDB Molecule:outer membrane protein oprg; PDBTitle: crystal structure of the outer membrane protein oprg from2 pseudomonas aeruginosa 25 c3nsgA_ not modelled 81.3 10 PDB header:membrane protein Chain: A: PDB Molecule:outer membrane protein f; PDBTitle: crystal structure of ompf, an outer membrane protein from salmonella2 typhi 26 c1fw3A_ not modelled 79.6 12 PDB header:hydrolase, membrane protein Chain: A: PDB Molecule:outer membrane phospholipase a; PDBTitle: outer membrane phospholipase a from escherichia coli 27 c3brzA_ not modelled 69.8 11 PDB header:transport protein Chain: A: PDB Molecule:todx; PDBTitle: crystal structure of the pseudomonas putida toluene2 transporter todx 28 c2lhfA_ not modelled 67.7 15 PDB header:membrane protein Chain: A: PDB Molecule:outer membrane protein h1; PDBTitle: solution structure of outer membrane protein h (oprh) from p.2 aeruginosa in dhpc micelles 29 c2y0kA_ not modelled 65.7 8 PDB header:transport protein Chain: A: PDB Molecule:pyroglutatmate porin opdo; PDBTitle: crystal structure of pseudomonas aeruginosa opdo 30 c3rbhC_ not modelled 61.0 11 PDB header:transport protein Chain: C: PDB Molecule:alginate production protein alge; PDBTitle: structure of alginate export protein alge from pseudomonas aeruginosa 31 c3kvnA_ not modelled 58.7 13 PDB header:hydrolase Chain: A: PDB Molecule:esterase esta; PDBTitle: crystal structure of the full-length autotransporter esta from2 pseudomonas aeruginosa 32 d2zfga1 not modelled 53.4 9 Fold:Transmembrane beta-barrels Superfamily:Porins Family:Porin 33 d3prna_ not modelled 51.4 13 Fold:Transmembrane beta-barrels Superfamily:Porins Family:Porin 34 c1qd6C_ not modelled 45.4 12 PDB header:membrane protein Chain: C: PDB Molecule:protein (outer membrane phospholipase (ompla)); PDBTitle: outer membrane phospholipase a from escherichia coli 35 d1a0tp_ not modelled 45.2 11 Fold:Transmembrane beta-barrels Superfamily:Porins Family:Maltoporin-like 36 d2jnaa1 not modelled 42.8 38 Fold:Dodecin subunit-like Superfamily:YdgH-like Family:YdgH-like 37 c3bryB_ not modelled 39.6 15 PDB header:transport protein Chain: B: PDB Molecule:tbux; PDBTitle: crystal structure of the ralstonia pickettii toluene2 transporter tbux 38 c2o4vA_ not modelled 38.7 13 PDB header:membrane protein Chain: A: PDB Molecule:porin p; PDBTitle: an arginine ladder in oprp mediates phosphate specific transfer across2 the outer membrane 39 c2odjA_ not modelled 38.3 18 PDB header:membrane protein Chain: A: PDB Molecule:porin d; PDBTitle: crystal structure of the outer membrane protein oprd from pseudomonas2 aeruginosa 40 d1k28d2 not modelled 32.6 14 Fold:Phage tail proteins Superfamily:Phage tail proteins Family:Baseplate protein-like 41 d1uynx_ not modelled 30.0 10 Fold:Transmembrane beta-barrels Superfamily:Autotransporter Family:Autotransporter 42 c2x4mD_ not modelled 22.5 13 PDB header:hydrolase Chain: D: PDB Molecule:coagulase/fibrinolysin; PDBTitle: yersinia pestis plasminogen activator pla 43 c1pdjF_ not modelled 19.4 14 PDB header:structural protein Chain: F: PDB Molecule:baseplate structural protein gp27; PDBTitle: fitting of gp27 into cryoem reconstruction of bacteriophage2 t4 baseplate 44 d2vdfa1 not modelled 17.6 13 Fold:Transmembrane beta-barrels Superfamily:OMPT-like Family:Outer membrane adhesin/invasin OpcA 45 c2qtkB_ not modelled 17.3 8 PDB header:membrane protein Chain: B: PDB Molecule:probable porin; PDBTitle: crystal structure of the outer membrane protein opdk from2 pseudomonas aeruginosa 46 d1i78a_ not modelled 16.1 14 Fold:Transmembrane beta-barrels Superfamily:OMPT-like Family:Outer membrane protease OMPT 47 d1af6a_ not modelled 15.8 13 Fold:Transmembrane beta-barrels Superfamily:Porins Family:Maltoporin-like 48 c2k4tA_ not modelled 12.4 11 PDB header:membrane protein,apoptosis Chain: A: PDB Molecule:voltage-dependent anion-selective channel PDBTitle: solution structure of human vdac-1 in ldao micelles 49 c2k3aA_ not modelled 11.3 25 PDB header:hydrolase Chain: A: PDB Molecule:chap domain protein; PDBTitle: nmr solution structure of staphylococcus saprophyticus chap2 (cysteine, histidine-dependent amidohydrolases/peptidases)3 domain protein. northeast structural genomics consortium4 target syr11 50 d2noca1 not modelled 11.2 17 Fold:Dodecin subunit-like Superfamily:YdgH-like Family:YdgH-like 51 c1e1hC_ not modelled 10.4 29 PDB header:hydrolase Chain: C: PDB Molecule:botulinum neurotoxin type a light chain; PDBTitle: crystal structure of recombinant botulinum neurotoxin type2 a light chain, self-inhibiting zn endopeptidase. 52 c1xkwA_ not modelled 10.1 16 PDB header:membrane protein Chain: A: PDB Molecule:fe(iii)-pyochelin receptor; PDBTitle: pyochelin outer membrane receptor fpta from pseudomonas2 aeruginosa 53 d2gufa1 not modelled 9.9 13 Fold:Transmembrane beta-barrels Superfamily:Porins Family:Ligand-gated protein channel 54 c3k3qB_ not modelled 9.9 29 PDB header:immune system Chain: B: PDB Molecule:botulinum neurotoxin type a; PDBTitle: crystal structure of a llama antibody complexed with the c.2 botulinum neurotoxin serotype a catalytic domain 55 d2jdih2 not modelled 9.8 32 Fold:Epsilon subunit of F1F0-ATP synthase N-terminal domain Superfamily:Epsilon subunit of F1F0-ATP synthase N-terminal domain Family:Epsilon subunit of F1F0-ATP synthase N-terminal domain 56 c2kx7A_ not modelled 7.7 25 PDB header:protein binding Chain: A: PDB Molecule:sensor-like histidine kinase yojn; PDBTitle: solution structure of the e.coli rcsd-abl domain (residues 688-795) 57 d1fepa_ not modelled 7.2 9 Fold:Transmembrane beta-barrels Superfamily:Porins Family:Ligand-gated protein channel 58 d1wdds_ not modelled 7.2 24 Fold:RuBisCO, small subunit Superfamily:RuBisCO, small subunit Family:RuBisCO, small subunit 59 c3jtyB_ not modelled 7.2 13 PDB header:transport protein Chain: B: PDB Molecule:benf-like porin; PDBTitle: crystal structure of a benf-like porin from pseudomonas fluorescens2 pf-5 60 d2mpra_ not modelled 6.7 14 Fold:Transmembrane beta-barrels Superfamily:Porins Family:Maltoporin-like 61 d1jeta_ not modelled 6.7 14 Fold:Periplasmic binding protein-like II Superfamily:Periplasmic binding protein-like II Family:Phosphate binding protein-like 62 c2y0lA_ not modelled 6.2 8 PDB header:transport protein Chain: A: PDB Molecule:cis-aconitate porin opdh; PDBTitle: crystal structure of pseudomonas aeruginosa opdo 63 d3bona1 not modelled 5.9 29 Fold:Zincin-like Superfamily:Metalloproteases ("zincins"), catalytic domain Family:Clostridium neurotoxins, catalytic domain 64 c2ilpA_ not modelled 5.8 29 PDB header:hydrolase Chain: A: PDB Molecule:botulinum neurotoxin a light-chain; PDBTitle: clostridium botulinum serotype a light chain inhibited by 4-2 chlorocinnamic hydroxamate 65 c1z7hA_ not modelled 5.4 50 PDB header:hydrolase Chain: A: PDB Molecule:tetanus toxin light chain; PDBTitle: 2.3 angstrom crystal structure of tetanus neurotoxin light2 chain 66 c2a97B_ not modelled 5.4 29 PDB header:hydrolase Chain: B: PDB Molecule:botulinum neurotoxin type f; PDBTitle: crystal structure of catalytic domain of clostridium2 botulinum neurotoxin serotype f 67 c2qn0A_ not modelled 5.4 21 PDB header:toxin Chain: A: PDB Molecule:neurotoxin; PDBTitle: structure of botulinum neurotoxin serotype c1 light chain2 protease

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0