Email l.a.kelley@imperial.ac.uk Description P0A6F3 Date Thu Jan 5 11:03:00 GMT 2012 Unique Job ID dc7ad51db283c485

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 c1glbG_ 100.0 100 PDB header:phosphotransferase Chain: G: PDB Molecule:glycerol kinase; PDBTitle: structure of the regulatory complex of escherichia coli iiiglc with2 glycerol kinase 2 c2d4wA_ 100.0 53 PDB header:transferase Chain: A: PDB Molecule:glycerol kinase; PDBTitle: crystal structure of glycerol kinase from cellulomonas sp.2 nt3060 3 c3flcX_ 100.0 56 PDB header:transferase Chain: X: PDB Molecule:glycerol kinase; PDBTitle: crystal structure of the his-tagged h232r mutant of glycerol kinase2 from enterococcus casseliflavus with glycerol 4 c3ezwD_ 100.0 100 PDB header:transferase Chain: D: PDB Molecule:glycerol kinase; PDBTitle: crystal structure of a hyperactive escherichia coli glycerol kinase2 mutant gly230 --> asp obtained using microfluidic crystallization3 devices 5 c3g25B_ 100.0 60 PDB header:transferase Chain: B: PDB Molecule:glycerol kinase; PDBTitle: 1.9 angstrom crystal structure of glycerol kinase (glpk) from2 staphylococcus aureus in complex with glycerol. 6 c2zf5O_ 100.0 57 PDB header:transferase Chain: O: PDB Molecule:glycerol kinase; PDBTitle: crystal structure of highly thermostable glycerol kinase from a2 hyperthermophilic archaeon 7 c2dpnB_ 100.0 51 PDB header:transferase Chain: B: PDB Molecule:glycerol kinase; PDBTitle: crystal structure of the glycerol kinase from thermus2 thermophilus hb8 8 c2w40C_ 100.0 41 PDB header:transferase Chain: C: PDB Molecule:glycerol kinase, putative; PDBTitle: crystal structure of plasmodium falciparum glycerol kinase2 with bound glycerol 9 c1xupO_ 100.0 57 PDB header:transferase Chain: O: PDB Molecule:glycerol kinase; PDBTitle: enterococcus casseliflavus glycerol kinase complexed with glycerol 10 c3gbtA_ 100.0 23 PDB header:transferase Chain: A: PDB Molecule:gluconate kinase; PDBTitle: crystal structure of gluconate kinase from lactobacillus acidophilus 11 c3hz6A_ 100.0 20 PDB header:transferase Chain: A: PDB Molecule:xylulokinase; PDBTitle: crystal structure of xylulokinase from chromobacterium violaceum 12 c3ifrB_ 100.0 20 PDB header:transferase Chain: B: PDB Molecule:carbohydrate kinase, fggy; PDBTitle: the crystal structure of xylulose kinase from rhodospirillum rubrum 13 c2nlxA_ 100.0 22 PDB header:transferase Chain: A: PDB Molecule:xylulose kinase; PDBTitle: crystal structure of the apo e. coli xylulose kinase 14 c3gg4B_ 100.0 21 PDB header:transferase Chain: B: PDB Molecule:glycerol kinase; PDBTitle: the crystal structure of glycerol kinase from yersinia2 pseudotuberculosis 15 c3jvpA_ 100.0 20 PDB header:transferase Chain: A: PDB Molecule:ribulokinase; PDBTitle: crystal structure of ribulokinase from bacillus halodurans 16 c2cgkB_ 100.0 18 PDB header:transferase Chain: B: PDB Molecule:l-rhamnulose kinase; PDBTitle: crystal structure of l-rhamnulose kinase from escherichia2 coli in an open uncomplexed conformation. 17 c3i8bA_ 100.0 17 PDB header:transferase Chain: A: PDB Molecule:xylulose kinase; PDBTitle: the crystal structure of xylulose kinase from2 bifidobacterium adolescentis 18 c3h6eB_ 100.0 13 PDB header:transferase Chain: B: PDB Molecule:carbohydrate kinase, fggy; PDBTitle: the crystal structure of a carbohydrate kinase from novosphingobium2 aromaticivorans 19 d2p3ra1 100.0 100 Fold:Ribonuclease H-like motif Superfamily:Actin-like ATPase domain Family:Glycerol kinase 20 d1r59o1 100.0 58 Fold:Ribonuclease H-like motif Superfamily:Actin-like ATPase domain Family:Glycerol kinase 21 d2p3ra2 not modelled 100.0 100 Fold:Ribonuclease H-like motif Superfamily:Actin-like ATPase domain Family:Glycerol kinase 22 d1r59o2 not modelled 100.0 57 Fold:Ribonuclease H-like motif Superfamily:Actin-like ATPase domain Family:Glycerol kinase 23 d1huxa_ not modelled 99.5 20 Fold:Ribonuclease H-like motif Superfamily:Actin-like ATPase domain Family:BadF/BadG/BcrA/BcrD-like 24 c3h1qB_ not modelled 99.5 19 PDB header:structural protein Chain: B: PDB Molecule:ethanolamine utilization protein eutj; PDBTitle: crystal structure of ethanolamine utilization protein eutj from2 carboxydothermus hydrogenoformans 25 c2e2pA_ not modelled 99.2 19 PDB header:transferase Chain: A: PDB Molecule:hexokinase; PDBTitle: crystal structure of sulfolobus tokodaii hexokinase in2 complex with adp 26 c2qm1D_ not modelled 99.2 20 PDB header:transferase Chain: D: PDB Molecule:glucokinase; PDBTitle: crystal structure of glucokinase from enterococcus faecalis 27 c2ap1A_ not modelled 99.1 16 PDB header:transferase Chain: A: PDB Molecule:putative regulator protein; PDBTitle: crystal structure of the putative regulatory protein 28 d2ewsa1 not modelled 99.1 21 Fold:Ribonuclease H-like motif Superfamily:Actin-like ATPase domain Family:Fumble-like 29 c3eo3B_ not modelled 99.1 22 PDB header:isomerase, transferase Chain: B: PDB Molecule:bifunctional udp-n-acetylglucosamine 2-epimerase/n- PDBTitle: crystal structure of the n-acetylmannosamine kinase domain of human2 gne protein 30 c1e4gT_ not modelled 99.0 13 PDB header:bacterial cell division Chain: T: PDB Molecule:cell division protein ftsa; PDBTitle: ftsa (atp-bound form) from thermotoga maritima 31 c2ivoC_ not modelled 99.0 16 PDB header:hydrolase Chain: C: PDB Molecule:up1; PDBTitle: structure of up1 protein 32 d1zc6a1 not modelled 99.0 18 Fold:Ribonuclease H-like motif Superfamily:Actin-like ATPase domain Family:BadF/BadG/BcrA/BcrD-like 33 c3enoB_ not modelled 98.9 18 PDB header:hydrolase/unknown function Chain: B: PDB Molecule:putative o-sialoglycoprotein endopeptidase; PDBTitle: crystal structure of pyrococcus furiosus pcc1 in complex2 with thermoplasma acidophilum kae1 34 c2aa4B_ not modelled 98.7 24 PDB header:transferase Chain: B: PDB Molecule:putative n-acetylmannosamine kinase; PDBTitle: crystal structure of escherichia coli putative n-2 acetylmannosamine kinase, new york structural genomics3 consortium 35 c1zc6A_ not modelled 98.7 18 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:probable n-acetylglucosamine kinase; PDBTitle: crystal structure of putative n-acetylglucosamine kinase from2 chromobacterium violaceum. northeast structural genomics target3 cvr23. 36 d1z05a3 not modelled 98.7 11 Fold:Ribonuclease H-like motif Superfamily:Actin-like ATPase domain Family:ROK 37 c3r8eA_ not modelled 98.6 18 PDB header:transferase Chain: A: PDB Molecule:hypothetical sugar kinase; PDBTitle: crystal structure of a hypothetical sugar kinase (chu_1875) from2 cytophaga hutchinsonii atcc 33406 at 1.65 a resolution 38 d2hoea3 not modelled 98.6 13 Fold:Ribonuclease H-like motif Superfamily:Actin-like ATPase domain Family:ROK 39 c2ch5D_ not modelled 98.5 10 PDB header:transferase Chain: D: PDB Molecule:nagk protein; PDBTitle: crystal structure of human n-acetylglucosamine kinase in2 complex with n-acetylglucosamine 40 d2ch5a2 not modelled 98.4 14 Fold:Ribonuclease H-like motif Superfamily:Actin-like ATPase domain Family:BadF/BadG/BcrA/BcrD-like 41 c1z05A_ not modelled 98.4 11 PDB header:transcription Chain: A: PDB Molecule:transcriptional regulator, rok family; PDBTitle: crystal structure of the rok family transcriptional regulator, homolog2 of e.coli mlc protein. 42 d1z6ra2 not modelled 98.4 17 Fold:Ribonuclease H-like motif Superfamily:Actin-like ATPase domain Family:ROK 43 c3vgkB_ not modelled 98.4 16 PDB header:transferase Chain: B: PDB Molecule:glucokinase; PDBTitle: crystal structure of a rok family glucokinase from streptomyces2 griseus 44 c3htvA_ not modelled 98.2 10 PDB header:transferase Chain: A: PDB Molecule:d-allose kinase; PDBTitle: crystal structure of d-allose kinase (np_418508.1) from escherichia2 coli k12 at 1.95 a resolution 45 c1dkgD_ not modelled 98.2 15 PDB header:complex (hsp24/hsp70) Chain: D: PDB Molecule:molecular chaperone dnak; PDBTitle: crystal structure of the nucleotide exchange factor grpe2 bound to the atpase domain of the molecular chaperone dnak 46 c1z6rC_ not modelled 98.2 13 PDB header:transcription Chain: C: PDB Molecule:mlc protein; PDBTitle: crystal structure of mlc from escherichia coli 47 d2ap1a2 not modelled 98.1 14 Fold:Ribonuclease H-like motif Superfamily:Actin-like ATPase domain Family:ROK 48 c2hoeA_ not modelled 98.0 12 PDB header:transferase Chain: A: PDB Molecule:n-acetylglucosamine kinase; PDBTitle: crystal structure of n-acetylglucosamine kinase (tm1224) from2 thermotoga maritima at 2.46 a resolution 49 d1dkgd2 not modelled 98.0 15 Fold:Ribonuclease H-like motif Superfamily:Actin-like ATPase domain Family:Actin/HSP70 50 c2v7zA_ not modelled 98.0 17 PDB header:chaperone Chain: A: PDB Molecule:heat shock cognate 71 kda protein; PDBTitle: crystal structure of the 70-kda heat shock cognate protein2 from rattus norvegicus in post-atp hydrolysis state 51 d1woqa1 not modelled 97.9 14 Fold:Ribonuclease H-like motif Superfamily:Actin-like ATPase domain Family:ROK 52 d1q18a1 not modelled 97.9 22 Fold:Ribonuclease H-like motif Superfamily:Actin-like ATPase domain Family:Glucokinase 53 c2v7yA_ not modelled 97.9 18 PDB header:chaperone Chain: A: PDB Molecule:chaperone protein dnak; PDBTitle: crystal structure of the molecular chaperone dnak from2 geobacillus kaustophilus hta426 in post-atp hydrolysis3 state 54 c1hpmA_ not modelled 97.9 16 PDB header:hydrolase (acting on acid anhydrides) Chain: A: PDB Molecule:44k atpase fragment (n-terminal) of 7o kd heat- PDBTitle: how potassium affects the activity of the molecular2 chaperone hsc70. ii. potassium binds specifically in the3 atpase active site 55 c3d2fC_ not modelled 97.8 14 PDB header:chaperone Chain: C: PDB Molecule:heat shock protein homolog sse1; PDBTitle: crystal structure of a complex of sse1p and hsp70 56 d2aa4a1 not modelled 97.8 16 Fold:Ribonuclease H-like motif Superfamily:Actin-like ATPase domain Family:ROK 57 c2gupA_ not modelled 97.8 18 PDB header:transferase Chain: A: PDB Molecule:rok family protein; PDBTitle: structural genomics, the crystal structure of a rok family protein2 from streptococcus pneumoniae tigr4 in complex with sucrose 58 c1jcgA_ not modelled 97.8 15 PDB header:structural protein Chain: A: PDB Molecule:rod shape-determining protein mreb; PDBTitle: mreb from thermotoga maritima, amppnp 59 d1sz2a1 not modelled 97.8 20 Fold:Ribonuclease H-like motif Superfamily:Actin-like ATPase domain Family:Glucokinase 60 d1jcea2 not modelled 97.8 20 Fold:Ribonuclease H-like motif Superfamily:Actin-like ATPase domain Family:Actin/HSP70 61 d1e4ft1 not modelled 97.7 13 Fold:Ribonuclease H-like motif Superfamily:Actin-like ATPase domain Family:Actin/HSP70 62 d2gupa1 not modelled 97.7 24 Fold:Ribonuclease H-like motif Superfamily:Actin-like ATPase domain Family:ROK 63 c3en9B_ not modelled 97.7 17 PDB header:hydrolase Chain: B: PDB Molecule:o-sialoglycoprotein endopeptidase/protein kinase; PDBTitle: structure of the methanococcus jannaschii kae1-bud32 fusion2 protein 64 d2e8aa2 not modelled 97.7 19 Fold:Ribonuclease H-like motif Superfamily:Actin-like ATPase domain Family:Actin/HSP70 65 c3mcpA_ not modelled 97.7 10 PDB header:transferase Chain: A: PDB Molecule:glucokinase; PDBTitle: crystal structure of glucokinase (bdi_1628) from parabacteroides2 distasonis atcc 8503 at 3.00 a resolution 66 d1bupa2 not modelled 97.7 16 Fold:Ribonuclease H-like motif Superfamily:Actin-like ATPase domain Family:Actin/HSP70 67 c3iucC_ not modelled 97.6 22 PDB header:chaperone Chain: C: PDB Molecule:heat shock 70kda protein 5 (glucose-regulated PDBTitle: crystal structure of the human 70kda heat shock protein 52 (bip/grp78) atpase domain in complex with adp 68 c1xc3A_ not modelled 97.5 13 PDB header:transferase Chain: A: PDB Molecule:putative fructokinase; PDBTitle: structure of a putative fructokinase from bacillus subtilis 69 c3cqyA_ not modelled 97.5 21 PDB header:transferase Chain: A: PDB Molecule:anhydro-n-acetylmuramic acid kinase; PDBTitle: crystal structure of a functionally unknown protein (so_1313) from2 shewanella oneidensis mr-1 70 c1woqB_ not modelled 97.4 11 PDB header:transferase Chain: B: PDB Molecule:inorganic polyphosphate/atp-glucomannokinase; PDBTitle: crystal structure of inorganic polyphosphate/atp-glucomannokinase from2 arthrobacter sp. strain km at 1.8 a resolution 71 d1xc3a1 not modelled 97.3 15 Fold:Ribonuclease H-like motif Superfamily:Actin-like ATPase domain Family:ROK 72 c2khoA_ not modelled 97.3 18 PDB header:chaperone Chain: A: PDB Molecule:heat shock protein 70; PDBTitle: nmr-rdc / xray structure of e. coli hsp70 (dnak) chaperone2 (1-605) complexed with adp and substrate 73 c2q2rA_ not modelled 97.2 15 PDB header:transferase Chain: A: PDB Molecule:glucokinase 1, putative; PDBTitle: trypanosoma cruzi glucokinase in complex with beta-d-glucose and adp 74 d1bg3a3 not modelled 97.0 14 Fold:Ribonuclease H-like motif Superfamily:Actin-like ATPase domain Family:Hexokinase 75 d2i7na2 not modelled 97.0 18 Fold:Ribonuclease H-like motif Superfamily:Actin-like ATPase domain Family:Fumble-like 76 c3qbwA_ not modelled 96.9 24 PDB header:transferase Chain: A: PDB Molecule:anhydro-n-acetylmuramic acid kinase; PDBTitle: crystal structure of pseudomonas aeruginosa 1,6-anhydro-n-2 actetylmuramic acid kinase (anmk) bound to adenosine diphosphate 77 d2i7pa1 not modelled 96.9 20 Fold:Ribonuclease H-like motif Superfamily:Actin-like ATPase domain Family:Fumble-like 78 d1bdga1 not modelled 96.9 18 Fold:Ribonuclease H-like motif Superfamily:Actin-like ATPase domain Family:Hexokinase 79 d1e4ft2 not modelled 96.9 14 Fold:Ribonuclease H-like motif Superfamily:Actin-like ATPase domain Family:Actin/HSP70 80 c3lm2B_ not modelled 96.9 13 PDB header:transferase Chain: B: PDB Molecule:putative kinase; PDBTitle: crystal structure of putative kinase. (17743352) from agrobacterium2 tumefaciens str. c58 (dupont) at 1.70 a resolution 81 c3tsuA_ not modelled 96.8 20 PDB header:transferase Chain: A: PDB Molecule:transcriptional regulatory protein; PDBTitle: crystal structure of e. coli hypf with amp-pnp and carbamoyl phosphate 82 c1sazA_ not modelled 96.8 15 PDB header:transferase Chain: A: PDB Molecule:probable butyrate kinase 2; PDBTitle: membership in the askha superfamily: enzymological2 properties and crystal structure of butyrate kinase 2 from3 thermotoga maritima 83 d1v4sa1 not modelled 96.7 11 Fold:Ribonuclease H-like motif Superfamily:Actin-like ATPase domain Family:Hexokinase 84 c2ychA_ not modelled 96.7 27 PDB header:cell cycle Chain: A: PDB Molecule:competence protein pilm; PDBTitle: pilm-piln type iv pilus biogenesis complex 85 c1bdgA_ not modelled 96.6 17 PDB header:hexokinase Chain: A: PDB Molecule:hexokinase; PDBTitle: hexokinase from schistosoma mansoni complexed with glucose 86 c3t69A_ not modelled 96.5 27 PDB header:transferase Chain: A: PDB Molecule:putative 2-dehydro-3-deoxygalactonokinase; PDBTitle: crystal structure of a putative 2-dehydro-3-deoxygalactonokinase2 protein from sinorhizobium meliloti 87 d1czan3 not modelled 96.4 16 Fold:Ribonuclease H-like motif Superfamily:Actin-like ATPase domain Family:Hexokinase 88 d1ig8a1 not modelled 96.4 19 Fold:Ribonuclease H-like motif Superfamily:Actin-like ATPase domain Family:Hexokinase 89 c3hm8D_ not modelled 96.3 16 PDB header:transferase Chain: D: PDB Molecule:hexokinase-3; PDBTitle: crystal structure of the c-terminal hexokinase domain of human hk3 90 c2d0oA_ not modelled 96.3 20 PDB header:chaperone Chain: A: PDB Molecule:diol dehydratase-reactivating factor large PDBTitle: strcuture of diol dehydratase-reactivating factor complexed2 with adp and mg2+ 91 c1v4sA_ not modelled 96.2 11 PDB header:transferase Chain: A: PDB Molecule:glucokinase isoform 2; PDBTitle: crystal structure of human glucokinase 92 d3bzka5 not modelled 96.2 20 Fold:Ribonuclease H-like motif Superfamily:Ribonuclease H-like Family:Tex RuvX-like domain-like 93 c1zbsA_ not modelled 96.2 16 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:hypothetical protein pg1100; PDBTitle: crystal structure of the putative n-acetylglucosamine kinase (pg1100)2 from porphyromonas gingivalis, northeast structural genomics target3 pgr18 94 c1ig8A_ not modelled 96.1 16 PDB header:transferase Chain: A: PDB Molecule:hexokinase pii; PDBTitle: crystal structure of yeast hexokinase pii with the correct2 amino acid sequence 95 d1czan1 not modelled 96.1 16 Fold:Ribonuclease H-like motif Superfamily:Actin-like ATPase domain Family:Hexokinase 96 d1bg3a1 not modelled 95.9 14 Fold:Ribonuclease H-like motif Superfamily:Actin-like ATPase domain Family:Hexokinase 97 c2i7pA_ not modelled 95.6 20 PDB header:transferase Chain: A: PDB Molecule:pantothenate kinase 3; PDBTitle: crystal structure of human pank3 in complex with accoa 98 d2e1za1 not modelled 95.5 16 Fold:Ribonuclease H-like motif Superfamily:Actin-like ATPase domain Family:Acetokinase-like 99 d1t6ca1 not modelled 95.4 15 Fold:Ribonuclease H-like motif Superfamily:Actin-like ATPase domain Family:Ppx/GppA phosphatase 100 d1g99a1 not modelled 95.3 15 Fold:Ribonuclease H-like motif Superfamily:Actin-like ATPase domain Family:Acetokinase-like 101 c1qhaA_ not modelled 95.2 13 PDB header:transferase Chain: A: PDB Molecule:protein (hexokinase); PDBTitle: human hexokinase type i complexed with atp analogue amp-pnp 102 c3smpA_ not modelled 95.1 17 PDB header:transferase Chain: A: PDB Molecule:pantothenate kinase 1; PDBTitle: monoclinic crystal structure of human pantothenate kinase 1 alpha 103 d2zgya2 not modelled 95.1 11 Fold:Ribonuclease H-like motif Superfamily:Actin-like ATPase domain Family:Actin/HSP70 104 d2aa4a2 not modelled 95.1 19 Fold:Ribonuclease H-like motif Superfamily:Actin-like ATPase domain Family:ROK 105 c1x3nA_ not modelled 95.0 14 PDB header:transferase Chain: A: PDB Molecule:propionate kinase; PDBTitle: crystal structure of amppnp bound propionate kinase (tdcd) from2 salmonella typhimurium 106 d1u6za2 not modelled 94.8 15 Fold:Ribonuclease H-like motif Superfamily:Actin-like ATPase domain Family:Ppx/GppA phosphatase 107 c2oceA_ not modelled 94.4 24 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:hypothetical protein pa5201; PDBTitle: crystal structure of tex family protein pa5201 from2 pseudomonas aeruginosa 108 d1ig8a2 not modelled 94.4 18 Fold:Ribonuclease H-like motif Superfamily:Actin-like ATPase domain Family:Hexokinase 109 c1mwmA_ not modelled 94.3 11 PDB header:structural protein Chain: A: PDB Molecule:parm; PDBTitle: parm from plasmid r1 adp form 110 c3nuwC_ not modelled 94.2 16 PDB header:transferase Chain: C: PDB Molecule:2-oxo-3-deoxygalactonate kinase; PDBTitle: crystal structure of 2-oxo-3-deoxygalactonate kinase from klebsiella2 pneumoniae 111 c2h3gX_ not modelled 94.1 16 PDB header:biosynthetic protein Chain: X: PDB Molecule:biosynthetic protein; PDBTitle: structure of the type iii pantothenate kinase (coax) from bacillus2 anthracis 112 d2ch5a1 not modelled 93.9 17 Fold:Ribonuclease H-like motif Superfamily:Actin-like ATPase domain Family:BadF/BadG/BcrA/BcrD-like 113 c3khyA_ not modelled 93.9 17 PDB header:transferase Chain: A: PDB Molecule:propionate kinase; PDBTitle: crystal structure of a propionate kinase from francisella2 tularensis subsp. tularensis schu s4 114 d1saza1 not modelled 93.9 13 Fold:Ribonuclease H-like motif Superfamily:Actin-like ATPase domain Family:Acetokinase-like 115 c1o1f4_ not modelled 93.7 10 PDB header:contractile protein Chain: 4: PDB Molecule:skeletal muscle actin; PDBTitle: molecular models of averaged rigor crossbridges from2 tomograms of insect flight muscle 116 c3aapA_ not modelled 93.4 25 PDB header:hydrolase Chain: A: PDB Molecule:ectonucleoside triphosphate diphosphohydrolase i; PDBTitle: crystal structure of lp1ntpdase from legionella pneumophila 117 d2e8aa1 not modelled 93.3 36 Fold:Ribonuclease H-like motif Superfamily:Actin-like ATPase domain Family:Actin/HSP70 118 c1zxoB_ not modelled 93.3 19 PDB header:unknown function Chain: B: PDB Molecule:conserved hypothetical protein q8a1p1; PDBTitle: x-ray crystal structure of protein q8a1p1 from bacteroides2 thetaiotaomicron. northeast structural genomics consortium3 target btr25. 119 d2d0oa2 not modelled 93.1 20 Fold:Ribonuclease H-like motif Superfamily:Actin-like ATPase domain Family:ATPase domain of dehydratase reactivase alpha subunit 120 c2floA_ not modelled 92.9 16 PDB header:hydrolase Chain: A: PDB Molecule:exopolyphosphatase; PDBTitle: crystal structure of exopolyphosphatase (ppx) from e. coli o157:h7