Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	I	I	K	K	S	G	G	R	W	Q	L	S	L	L	A	S	V	V	I	S	A	F	F	L	N	T	A	Y	A	W	Q	Q	E	Y	I	V	D	T	Q	P	G	L	S	T	E	R	Y	T	W	D	S	D	H	Q	P	D	Y	N	D
Secondary structure
SS confidence
Disorder		?	?			?	?	?																															?				?													?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		I	L	S	Q	R	I	Q	S	S	Q	R	A	L	G	L	E	V	N	L	A	E	E	T	P	V	D	V	T	S	S	M	S	M	G	W	N	F	P	L	Y	E	Q	V	T	T	G	P	V	A	A	L	H	Y	D	G	T	T	T	S	M
Secondary structure
SS confidence
Disorder								?	?	?	?	?	?	?		?	?	?	?	?	?	?	?	?	?	?	?	?	?	?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		Y	N	E	F	G	D	S	T	T	T	L	T	D	P	L	W	H	A	S	V	S	T	L	G	W	R	V	D	S	R	L	G	D	L	R	P	W	A	Q	I	S	Y	N	Q	Q	F	G	E	N	I	W	K	A	Q	S	G	L	S	R	M
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?					?	?		?												?																			?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.
Sequence		T	A	T	N	Q	N	G	N	W	L	D	V	T	V	G	A	D	M	L	L	N	Q	N	I	A	A	Y	A	A	L	T	Q	A	E	N	T	T	N	N	S	D	Y	L	Y	T	M	G	V	S	A	R	F
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?																									?	?	?	?	?	?	?	?	?	?	?											?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 3slj chain A Region: 9 - 232 Aligned: 224 Modelled: 224 Confidence: 100.0% Identity: 11% PDB header:protein transport Chain: A: PDB Molecule:serine protease espp; PDBTitle: pre-cleavage structure of the autotransporter espp - n1023a mutant Phyre2 PDB 3kvn chain A Region: 10 - 232 Aligned: 222 Modelled: 223 Confidence: 100.0% Identity: 12% PDB header:hydrolase Chain: A: PDB Molecule:esterase esta; PDBTitle: crystal structure of the full-length autotransporter esta from2 pseudomonas aeruginosa Phyre2 PDB 2qom chain B Region: 10 - 232 Aligned: 221 Modelled: 223 Confidence: 100.0% Identity: 11% PDB header:hydrolase Chain: B: PDB Molecule:serine protease espp; PDBTitle: the crystal structure of the e.coli espp autotransporter beta-domain. Phyre2 PDB 3qq2 chain C Region: 10 - 232 Aligned: 206 Modelled: 208 Confidence: 100.0% Identity: 17% PDB header:membrane protein/protein transport Chain: C: PDB Molecule:brka autotransporter; PDBTitle: crystal structure of the beta domain of the bordetella autotransporter2 brka Phyre2 PDB 1uyn chain X Region: 10 - 232 Aligned: 209 Modelled: 223 Confidence: 100.0% Identity: 12% Fold: Transmembrane beta-barrels Superfamily: Autotransporter Family: Autotransporter Phyre2 PDB 3aeh chain B Region: 9 - 232 Aligned: 197 Modelled: 210 Confidence: 100.0% Identity: 12% PDB header:hydrolase Chain: B: PDB Molecule:hemoglobin-binding protease hbp autotransporter; PDBTitle: integral membrane domain of autotransporter hbp Phyre2 PDB 2k0l chain A Region: 68 - 232 Aligned: 152 Modelled: 165 Confidence: 98.8% Identity: 13% PDB header:membrane protein Chain: A: PDB Molecule:outer membrane protein a; PDBTitle: nmr structure of the transmembrane domain of the outer2 membrane protein a from klebsiella pneumoniae in dhpc3 micelles. Phyre2 PDB 1qjp chain A Region: 85 - 232 Aligned: 116 Modelled: 129 Confidence: 98.7% Identity: 17% Fold: Transmembrane beta-barrels Superfamily: OMPA-like Family: Outer membrane protein Phyre2 PDB 2f1t chain B Region: 84 - 232 Aligned: 141 Modelled: 147 Confidence: 98.7% Identity: 12% PDB header:membrane protein Chain: B: PDB Molecule:outer membrane protein w; PDBTitle: outer membrane protein ompw Phyre2 PDB 1g90 chain A Region: 78 - 232 Aligned: 137 Modelled: 155 Confidence: 98.7% Identity: 14% Fold: Transmembrane beta-barrels Superfamily: OMPA-like Family: Outer membrane protein Phyre2 PDB 3qra chain A Region: 78 - 232 Aligned: 133 Modelled: 145 Confidence: 98.6% Identity: 8% PDB header:cell invasion Chain: A: PDB Molecule:attachment invasion locus protein; PDBTitle: the crystal structure of ail, the attachment invasion locus protein of2 yersinia pestis Phyre2 PDB 3nb3 chain C Region: 84 - 232 Aligned: 117 Modelled: 124 Confidence: 98.6% Identity: 18% PDB header:virus Chain: C: PDB Molecule:outer membrane protein a; PDBTitle: the host outer membrane proteins ompa and ompc are packed at specific2 sites in the shigella phage sf6 virion as structural components Phyre2 PDB 2x27 chain X Region: 85 - 232 Aligned: 142 Modelled: 148 Confidence: 98.6% Identity: 15% PDB header:membrane protein Chain: X: PDB Molecule:outer membrane protein oprg; PDBTitle: crystal structure of the outer membrane protein oprg from2 pseudomonas aeruginosa Phyre2 PDB 1qj8 chain A Region: 85 - 232 Aligned: 130 Modelled: 148 Confidence: 98.6% Identity: 12% Fold: Transmembrane beta-barrels Superfamily: OMPA-like Family: Outer membrane protein Phyre2 PDB 1p4t chain A Region: 83 - 232 Aligned: 125 Modelled: 134 Confidence: 98.5% Identity: 16% Fold: Transmembrane beta-barrels Superfamily: OMPA-like Family: Outer membrane protein Phyre2 PDB 2jmm chain A Region: 83 - 232 Aligned: 126 Modelled: 135 Confidence: 98.2% Identity: 16% PDB header:membrane protein Chain: A: PDB Molecule:outer membrane protein a; PDBTitle: nmr solution structure of a minimal transmembrane beta-2 barrel platform protein Phyre2 PDB 2iwv chain D Region: 91 - 232 Aligned: 124 Modelled: 142 Confidence: 98.0% Identity: 12% PDB header:ion channel Chain: D: PDB Molecule:outer membrane protein g; PDBTitle: structure of the monomeric outer membrane porin ompg in the2 open and closed conformation Phyre2 PDB 1i78 chain A Region: 84 - 232 Aligned: 149 Modelled: 149 Confidence: 97.9% Identity: 17% Fold: Transmembrane beta-barrels Superfamily: OMPT-like Family: Outer membrane protease OMPT Phyre2 PDB 2x4m chain D Region: 36 - 232 Aligned: 183 Modelled: 183 Confidence: 97.7% Identity: 15% PDB header:hydrolase Chain: D: PDB Molecule:coagulase/fibrinolysin; PDBTitle: yersinia pestis plasminogen activator pla Phyre2 PDB 2lhf chain A Region: 99 - 232 Aligned: 128 Modelled: 128 Confidence: 97.7% Identity: 11% PDB header:membrane protein Chain: A: PDB Molecule:outer membrane protein h1; PDBTitle: solution structure of outer membrane protein h (oprh) from p.2 aeruginosa in dhpc micelles Phyre2 1 c3sljA_ 2 c3kvnA_ 3 c2qomB_ 4 c3qq2C_ 5 d1uynx_ 6 c3aehB_ 7 c2k0lA_ 8 d1qjpa_ 9 c2f1tB_ 10 d1g90a_ 11 c3qraA_ 12 c3nb3C_ 13 c2x27X_ 14 d1qj8a_ 15 d1p4ta_ 16 c2jmmA_ 17 c2iwvD_ 18 d1i78a_ 19 c2x4mD_ 20 c2lhfA_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 c3sljA_ 100.0 11 PDB header:protein transport Chain: A: PDB Molecule:serine protease espp; PDBTitle: pre-cleavage structure of the autotransporter espp - n1023a mutant 2 c3kvnA_ 100.0 12 PDB header:hydrolase Chain: A: PDB Molecule:esterase esta; PDBTitle: crystal structure of the full-length autotransporter esta from2 pseudomonas aeruginosa 3 c2qomB_ 100.0 11 PDB header:hydrolase Chain: B: PDB Molecule:serine protease espp; PDBTitle: the crystal structure of the e.coli espp autotransporter beta-domain. 4 c3qq2C_ 100.0 17 PDB header:membrane protein/protein transport Chain: C: PDB Molecule:brka autotransporter; PDBTitle: crystal structure of the beta domain of the bordetella autotransporter2 brka 5 d1uynx_ 100.0 12 Fold:Transmembrane beta-barrels Superfamily:Autotransporter Family:Autotransporter 6 c3aehB_ 100.0 12 PDB header:hydrolase Chain: B: PDB Molecule:hemoglobin-binding protease hbp autotransporter; PDBTitle: integral membrane domain of autotransporter hbp 7 c2k0lA_ 98.8 13 PDB header:membrane protein Chain: A: PDB Molecule:outer membrane protein a; PDBTitle: nmr structure of the transmembrane domain of the outer2 membrane protein a from klebsiella pneumoniae in dhpc3 micelles. 8 d1qjpa_ 98.7 17 Fold:Transmembrane beta-barrels Superfamily:OMPA-like Family:Outer membrane protein 9 c2f1tB_ 98.7 12 PDB header:membrane protein Chain: B: PDB Molecule:outer membrane protein w; PDBTitle: outer membrane protein ompw 10 d1g90a_ 98.7 14 Fold:Transmembrane beta-barrels Superfamily:OMPA-like Family:Outer membrane protein 11 c3qraA_ 98.6 8 PDB header:cell invasion Chain: A: PDB Molecule:attachment invasion locus protein; PDBTitle: the crystal structure of ail, the attachment invasion locus protein of2 yersinia pestis 12 c3nb3C_ 98.6 18 PDB header:virus Chain: C: PDB Molecule:outer membrane protein a; PDBTitle: the host outer membrane proteins ompa and ompc are packed at specific2 sites in the shigella phage sf6 virion as structural components 13 c2x27X_ 98.6 15 PDB header:membrane protein Chain: X: PDB Molecule:outer membrane protein oprg; PDBTitle: crystal structure of the outer membrane protein oprg from2 pseudomonas aeruginosa 14 d1qj8a_ 98.6 12 Fold:Transmembrane beta-barrels Superfamily:OMPA-like Family:Outer membrane protein 15 d1p4ta_ 98.5 16 Fold:Transmembrane beta-barrels Superfamily:OMPA-like Family:Outer membrane protein 16 c2jmmA_ 98.2 16 PDB header:membrane protein Chain: A: PDB Molecule:outer membrane protein a; PDBTitle: nmr solution structure of a minimal transmembrane beta-2 barrel platform protein 17 c2iwvD_ 98.0 12 PDB header:ion channel Chain: D: PDB Molecule:outer membrane protein g; PDBTitle: structure of the monomeric outer membrane porin ompg in the2 open and closed conformation 18 d1i78a_ 97.9 17 Fold:Transmembrane beta-barrels Superfamily:OMPT-like Family:Outer membrane protease OMPT 19 c2x4mD_ 97.7 15 PDB header:hydrolase Chain: D: PDB Molecule:coagulase/fibrinolysin; PDBTitle: yersinia pestis plasminogen activator pla 20 c2lhfA_ 97.7 11 PDB header:membrane protein Chain: A: PDB Molecule:outer membrane protein h1; PDBTitle: solution structure of outer membrane protein h (oprh) from p.2 aeruginosa in dhpc micelles 21 c2wjqA_ not modelled 97.7 16 PDB header:transport protein Chain: A: PDB Molecule:probable n-acetylneuraminic acid outer membrane channel PDBTitle: nanc porin structure in hexagonal crystal form. 22 c3a2rX_ not modelled 97.1 15 PDB header:membrane protein Chain: X: PDB Molecule:outer membrane protein ii; PDBTitle: crystal structure of outer membrane protein porb from neisseria2 meningitidis 23 d2fgqx1 not modelled 97.1 16 Fold:Transmembrane beta-barrels Superfamily:Porins Family:Porin 24 d2pora_ not modelled 96.8 18 Fold:Transmembrane beta-barrels Superfamily:Porins Family:Porin 25 d3prna_ not modelled 96.5 16 Fold:Transmembrane beta-barrels Superfamily:Porins Family:Porin 26 d1osma_ not modelled 96.1 23 Fold:Transmembrane beta-barrels Superfamily:Porins Family:Porin 27 d1t16a_ not modelled 95.7 10 Fold:Transmembrane beta-barrels Superfamily:Porins Family:Outer membrane protein transport protein 28 d1phoa_ not modelled 95.4 17 Fold:Transmembrane beta-barrels Superfamily:Porins Family:Porin 29 c3dwoX_ not modelled 94.9 14 PDB header:membrane protein Chain: X: PDB Molecule:probable outer membrane protein; PDBTitle: crystal structure of a pseudomonas aeruginosa fadl homologue 30 c3nsgA_ not modelled 94.7 15 PDB header:membrane protein Chain: A: PDB Molecule:outer membrane protein f; PDBTitle: crystal structure of ompf, an outer membrane protein from salmonella2 typhi 31 d2zfga1 not modelled 93.7 13 Fold:Transmembrane beta-barrels Superfamily:Porins Family:Porin 32 c2iahA_ not modelled 90.1 8 PDB header:membrane protein Chain: A: PDB Molecule:ferripyoverdine receptor; PDBTitle: crystal structure of the ferripyoverdine receptor of the outer2 membrane of pseudomonas aeruginosa bound to ferripyoverdine. 33 c3brzA_ not modelled 89.2 12 PDB header:transport protein Chain: A: PDB Molecule:todx; PDBTitle: crystal structure of the pseudomonas putida toluene2 transporter todx 34 c3dzmB_ not modelled 89.0 16 PDB header:unknown function Chain: B: PDB Molecule:hypothetical conserved protein; PDBTitle: crystal structure of a major outer membrane protein from thermus2 thermophilus hb27 35 c2hdiA_ not modelled 82.0 20 PDB header:protein transport,antimicrobial protein Chain: A: PDB Molecule:colicin i receptor; PDBTitle: crystal structure of the colicin i receptor cir from e.coli in complex2 with receptor binding domain of colicin ia. 36 c1xkhC_ not modelled 81.8 8 PDB header:membrane protein Chain: C: PDB Molecule:ferripyoverdine receptor; PDBTitle: pyoverdine outer membrane receptor fpva from pseudomonas aeruginosa2 pao1 bound to pyoverdine 37 c1xkwA_ not modelled 69.9 12 PDB header:membrane protein Chain: A: PDB Molecule:fe(iii)-pyochelin receptor; PDBTitle: pyochelin outer membrane receptor fpta from pseudomonas2 aeruginosa 38 d2gufa1 not modelled 31.9 15 Fold:Transmembrane beta-barrels Superfamily:Porins Family:Ligand-gated protein channel 39 c2o4vA_ not modelled 28.5 16 PDB header:membrane protein Chain: A: PDB Molecule:porin p; PDBTitle: an arginine ladder in oprp mediates phosphate specific transfer across2 the outer membrane 40 d1fepa_ not modelled 26.5 17 Fold:Transmembrane beta-barrels Superfamily:Porins Family:Ligand-gated protein channel 41 d2gr7a1 not modelled 24.7 14 Fold:Pili subunits Superfamily:Pili subunits Family:YadA C-terminal domain-like 42 c2gr7C_ not modelled 24.7 14 PDB header:membrane protein Chain: C: PDB Molecule:adhesin; PDBTitle: hia 992-1098 43 c3cslB_ not modelled 23.1 10 PDB header:membrane protein/heme binding protein Chain: B: PDB Molecule:hasr protein; PDBTitle: structure of the serratia marcescens hemophore receptor hasr in2 complex with its hemophore hasa and heme 44 c3bryB_ not modelled 18.6 12 PDB header:transport protein Chain: B: PDB Molecule:tbux; PDBTitle: crystal structure of the ralstonia pickettii toluene2 transporter tbux 45 d2gr8a1 not modelled 15.2 14 Fold:Pili subunits Superfamily:Pili subunits Family:YadA C-terminal domain-like 46 c1fw3A_ not modelled 14.3 14 PDB header:hydrolase, membrane protein Chain: A: PDB Molecule:outer membrane phospholipase a; PDBTitle: outer membrane phospholipase a from escherichia coli 47 c3qlbA_ not modelled 12.8 13 PDB header:metal transport Chain: A: PDB Molecule:enantio-pyochelin receptor; PDBTitle: enantiopyochelin outer membrane tonb-dependent transporter from2 pseudomonas fluorescens bound to the ferri-enantiopyochelin 48 c3emoA_ not modelled 9.5 16 PDB header:membrane protein/cell adhesion Chain: A: PDB Molecule:hia (adhesin); PDBTitle: crystal structure of transmembrane hia 973-1098 49 d1kmoa_ not modelled 7.8 10 Fold:Transmembrane beta-barrels Superfamily:Porins Family:Ligand-gated protein channel 50 c2k4tA_ not modelled 7.6 10 PDB header:membrane protein,apoptosis Chain: A: PDB Molecule:voltage-dependent anion-selective channel PDBTitle: solution structure of human vdac-1 in ldao micelles 51 d1by5a_ not modelled 7.3 15 Fold:Transmembrane beta-barrels Superfamily:Porins Family:Ligand-gated protein channel 52 d2cxaa1 not modelled 6.3 17 Fold:Acyl-CoA N-acyltransferases (Nat) Superfamily:Acyl-CoA N-acyltransferases (Nat) Family:LFTR-like 53 c2cxaA_ not modelled 6.3 17 PDB header:transferase Chain: A: PDB Molecule:leucyl/phenylalanyl-trna-protein transferase; PDBTitle: crystal structure of leucyl/phenylalanyl-trna protein2 transferase from escherichia coli 54 c3qteA_ not modelled 6.3 57 PDB header:antimicrobial protein Chain: A: PDB Molecule:defensin-6; PDBTitle: crystal structure of human alpha-defensin 6 (h27w mutant)

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

---

Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0