Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	F	R	K	L	A	A	E	C	F	G	T	F	W	L	V	F	G	G	C	G	S	A	V	L	A	A	G	F	P	E	L	G	I	G	F	A	G	V	A	L	A	F	G	L	T	V	L	T	M	A	F	A	V	G	H	I	S	G	G
Secondary structure
SS confidence
Disorder		?																									?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		H	F	N	P	A	V	T	I	G	L	W	A	G	G	R	F	P	A	K	E	V	V	G	Y	V	I	A	Q	V	V	G	G	I	V	A	A	A	L	L	Y	L	I	A	S	G	K	T	G	F	D	A	A	A	S	G	F	A	S	N	G
Secondary structure
SS confidence
Disorder																																																?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		Y	G	E	H	S	P	G	G	Y	S	M	L	S	A	L	V	V	E	L	V	L	S	A	G	F	L	L	V	I	H	G	A	T	D	K	F	A	P	A	G	F	A	P	I	A	I	G	L	A	L	T	L	I	H	L	I	S	I	P	V
Secondary structure
SS confidence
Disorder																																					?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.
Sequence		T	N	T	S	V	N	P	A	R	S	T	A	V	A	I	F	Q	G	G	W	A	L	E	Q	L	W	F	F	W	V	V	P	I	V	G	G	I	I	G	G	L	I	Y	R	T	L	L	E	K	R	D
Secondary structure
SS confidence
Disorder		?	?																																														?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 1rc2 chain A Region: 1 - 231 Aligned: 231 Modelled: 231 Confidence: 100.0% Identity: 100% Fold: Aquaporin-like Superfamily: Aquaporin-like Family: Aquaporin-like Phyre2 PDB 3llq chain B Region: 1 - 229 Aligned: 227 Modelled: 229 Confidence: 100.0% Identity: 74% PDB header:membrane protein Chain: B: PDB Molecule:aquaporin z 2; PDBTitle: aquaporin structure from plant pathogen agrobacterium tumerfaciens Phyre2 PDB 2w2e chain A Region: 2 - 226 Aligned: 212 Modelled: 213 Confidence: 100.0% Identity: 32% PDB header:membrane protein Chain: A: PDB Molecule:aquaporin; PDBTitle: 1.15 angstrom crystal structure of p.pastoris aquaporin,2 aqy1, in a closed conformation at ph 3.5 Phyre2 PDB 1lda chain A Region: 1 - 230 Aligned: 229 Modelled: 230 Confidence: 100.0% Identity: 29% PDB header:transport protein Chain: A: PDB Molecule:glycerol uptake facilitator protein; PDBTitle: crystal structure of the e. coli glycerol facilitator (glpf) without2 substrate glycerol Phyre2 PDB 2f2b chain A Region: 1 - 228 Aligned: 221 Modelled: 221 Confidence: 100.0% Identity: 33% PDB header:membrane protein Chain: A: PDB Molecule:aquaporin aqpm; PDBTitle: crystal structure of integral membrane protein aquaporin aqpm at 1.68a2 resolution Phyre2 PDB 1fx8 chain A Region: 1 - 230 Aligned: 229 Modelled: 230 Confidence: 100.0% Identity: 29% Fold: Aquaporin-like Superfamily: Aquaporin-like Family: Aquaporin-like Phyre2 PDB 1j4n chain A Region: 1 - 231 Aligned: 220 Modelled: 231 Confidence: 100.0% Identity: 34% Fold: Aquaporin-like Superfamily: Aquaporin-like Family: Aquaporin-like Phyre2 PDB 3c02 chain A Region: 2 - 231 Aligned: 225 Modelled: 230 Confidence: 100.0% Identity: 26% PDB header:membrane protein Chain: A: PDB Molecule:aquaglyceroporin; PDBTitle: x-ray structure of the aquaglyceroporin from plasmodium falciparum Phyre2 PDB 1h6i chain A Region: 1 - 229 Aligned: 218 Modelled: 229 Confidence: 100.0% Identity: 37% Fold: Aquaporin-like Superfamily: Aquaporin-like Family: Aquaporin-like Phyre2 PDB 1ymg chain A domain 1 Region: 1 - 231 Aligned: 216 Modelled: 231 Confidence: 100.0% Identity: 35% Fold: Aquaporin-like Superfamily: Aquaporin-like Family: Aquaporin-like Phyre2 PDB 1ymg chain A Region: 1 - 231 Aligned: 216 Modelled: 231 Confidence: 100.0% Identity: 35% PDB header:membrane protein Chain: A: PDB Molecule:lens fiber major intrinsic protein; PDBTitle: the channel architecture of aquaporin o at 2.2 angstrom resolution Phyre2 PDB 3d9s chain B Region: 1 - 231 Aligned: 218 Modelled: 231 Confidence: 100.0% Identity: 36% PDB header:membrane protein Chain: B: PDB Molecule:aquaporin-5; PDBTitle: human aquaporin 5 (aqp5) - high resolution x-ray structure Phyre2 PDB 2d57 chain A Region: 1 - 229 Aligned: 218 Modelled: 220 Confidence: 100.0% Identity: 31% PDB header:transport protein Chain: A: PDB Molecule:aquaporin-4; PDBTitle: double layered 2d crystal structure of aquaporin-4 (aqp4m23) at 3.2 a2 resolution by electron crystallography Phyre2 PDB 3iyz chain A Region: 1 - 227 Aligned: 216 Modelled: 218 Confidence: 100.0% Identity: 31% PDB header:transport protein Chain: A: PDB Molecule:aquaporin-4; PDBTitle: structure of aquaporin-4 s180d mutant at 10.0 a resolution from2 electron micrograph Phyre2 PDB 2b5f chain D Region: 1 - 226 Aligned: 218 Modelled: 218 Confidence: 100.0% Identity: 28% PDB header:transport protein,membrane protein Chain: D: PDB Molecule:aquaporin; PDBTitle: crystal structure of the spinach aquaporin sopip2;1 in an2 open conformation to 3.9 resolution Phyre2 PDB 3gd8 chain A Region: 1 - 229 Aligned: 218 Modelled: 229 Confidence: 100.0% Identity: 31% PDB header:membrane protein Chain: A: PDB Molecule:aquaporin-4; PDBTitle: crystal structure of human aquaporin 4 at 1.8 and its mechanism of2 conductance Phyre2 PDB 3kcv chain G Region: 2 - 229 Aligned: 225 Modelled: 228 Confidence: 90.1% Identity: 12% PDB header:transport protein Chain: G: PDB Molecule:probable formate transporter 1; PDBTitle: structure of formate channel Phyre2 PDB 3klz chain E Region: 2 - 229 Aligned: 225 Modelled: 228 Confidence: 35.9% Identity: 12% PDB header:membrane protein Chain: E: PDB Molecule:putative formate transporter 1; PDBTitle: pentameric formate channel with formate bound Phyre2 PDB 1f0k chain A Region: 58 - 75 Aligned: 18 Modelled: 18 Confidence: 22.6% Identity: 22% Fold: UDP-Glycosyltransferase/glycogen phosphorylase Superfamily: UDP-Glycosyltransferase/glycogen phosphorylase Family: Peptidoglycan biosynthesis glycosyltransferase MurG Phyre2 PDB 1pn3 chain A Region: 58 - 75 Aligned: 18 Modelled: 18 Confidence: 13.4% Identity: 22% Fold: UDP-Glycosyltransferase/glycogen phosphorylase Superfamily: UDP-Glycosyltransferase/glycogen phosphorylase Family: Gtf glycosyltransferase Phyre2 1 d1rc2a_ 2 c3llqB_ 3 c2w2eA_ 4 c1ldaA_ 5 c2f2bA_ 6 d1fx8a_ 7 d1j4na_ 8 c3c02A_ 9 d1h6ia_ 10 d1ymga1 11 c1ymgA_ 12 c3d9sB_ 13 c2d57A_ 14 c3iyzA_ 15 c2b5fD_ 16 c3gd8A_ 17 c3kcvG_ 18 c3klzE_ 19 d1f0ka_ 20 d1pn3a_ 21 22 23 24 25 26 27 28 29 30 31 32 33

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 d1rc2a_ 100.0 100 Fold:Aquaporin-like Superfamily:Aquaporin-like Family:Aquaporin-like 2 c3llqB_ 100.0 74 PDB header:membrane protein Chain: B: PDB Molecule:aquaporin z 2; PDBTitle: aquaporin structure from plant pathogen agrobacterium tumerfaciens 3 c2w2eA_ 100.0 32 PDB header:membrane protein Chain: A: PDB Molecule:aquaporin; PDBTitle: 1.15 angstrom crystal structure of p.pastoris aquaporin,2 aqy1, in a closed conformation at ph 3.5 4 c1ldaA_ 100.0 29 PDB header:transport protein Chain: A: PDB Molecule:glycerol uptake facilitator protein; PDBTitle: crystal structure of the e. coli glycerol facilitator (glpf) without2 substrate glycerol 5 c2f2bA_ 100.0 33 PDB header:membrane protein Chain: A: PDB Molecule:aquaporin aqpm; PDBTitle: crystal structure of integral membrane protein aquaporin aqpm at 1.68a2 resolution 6 d1fx8a_ 100.0 29 Fold:Aquaporin-like Superfamily:Aquaporin-like Family:Aquaporin-like 7 d1j4na_ 100.0 34 Fold:Aquaporin-like Superfamily:Aquaporin-like Family:Aquaporin-like 8 c3c02A_ 100.0 26 PDB header:membrane protein Chain: A: PDB Molecule:aquaglyceroporin; PDBTitle: x-ray structure of the aquaglyceroporin from plasmodium falciparum 9 d1h6ia_ 100.0 37 Fold:Aquaporin-like Superfamily:Aquaporin-like Family:Aquaporin-like 10 d1ymga1 100.0 35 Fold:Aquaporin-like Superfamily:Aquaporin-like Family:Aquaporin-like 11 c1ymgA_ 100.0 35 PDB header:membrane protein Chain: A: PDB Molecule:lens fiber major intrinsic protein; PDBTitle: the channel architecture of aquaporin o at 2.2 angstrom resolution 12 c3d9sB_ 100.0 36 PDB header:membrane protein Chain: B: PDB Molecule:aquaporin-5; PDBTitle: human aquaporin 5 (aqp5) - high resolution x-ray structure 13 c2d57A_ 100.0 31 PDB header:transport protein Chain: A: PDB Molecule:aquaporin-4; PDBTitle: double layered 2d crystal structure of aquaporin-4 (aqp4m23) at 3.2 a2 resolution by electron crystallography 14 c3iyzA_ 100.0 31 PDB header:transport protein Chain: A: PDB Molecule:aquaporin-4; PDBTitle: structure of aquaporin-4 s180d mutant at 10.0 a resolution from2 electron micrograph 15 c2b5fD_ 100.0 28 PDB header:transport protein,membrane protein Chain: D: PDB Molecule:aquaporin; PDBTitle: crystal structure of the spinach aquaporin sopip2;1 in an2 open conformation to 3.9 resolution 16 c3gd8A_ 100.0 31 PDB header:membrane protein Chain: A: PDB Molecule:aquaporin-4; PDBTitle: crystal structure of human aquaporin 4 at 1.8 and its mechanism of2 conductance 17 c3kcvG_ 90.1 12 PDB header:transport protein Chain: G: PDB Molecule:probable formate transporter 1; PDBTitle: structure of formate channel 18 c3klzE_ 35.9 12 PDB header:membrane protein Chain: E: PDB Molecule:putative formate transporter 1; PDBTitle: pentameric formate channel with formate bound 19 d1f0ka_ 22.6 22 Fold:UDP-Glycosyltransferase/glycogen phosphorylase Superfamily:UDP-Glycosyltransferase/glycogen phosphorylase Family:Peptidoglycan biosynthesis glycosyltransferase MurG 20 d1pn3a_ 13.4 22 Fold:UDP-Glycosyltransferase/glycogen phosphorylase Superfamily:UDP-Glycosyltransferase/glycogen phosphorylase Family:Gtf glycosyltransferase 21 d1rrva_ not modelled 12.3 11 Fold:UDP-Glycosyltransferase/glycogen phosphorylase Superfamily:UDP-Glycosyltransferase/glycogen phosphorylase Family:Gtf glycosyltransferase 22 d1iira_ not modelled 11.5 22 Fold:UDP-Glycosyltransferase/glycogen phosphorylase Superfamily:UDP-Glycosyltransferase/glycogen phosphorylase Family:Gtf glycosyltransferase 23 c1s4iC_ not modelled 8.9 71 PDB header:oxidoreductase Chain: C: PDB Molecule:superoxide dismutase-like protein yojm; PDBTitle: crystal structure of a sod-like protein from bacillus subtilis 24 d1esoa_ not modelled 8.0 67 Fold:Immunoglobulin-like beta-sandwich Superfamily:Cu,Zn superoxide dismutase-like Family:Cu,Zn superoxide dismutase-like 25 d1eqwa_ not modelled 7.5 57 Fold:Immunoglobulin-like beta-sandwich Superfamily:Cu,Zn superoxide dismutase-like Family:Cu,Zn superoxide dismutase-like 26 d1oala_ not modelled 6.9 67 Fold:Immunoglobulin-like beta-sandwich Superfamily:Cu,Zn superoxide dismutase-like Family:Cu,Zn superoxide dismutase-like 27 c3d0qB_ not modelled 6.6 17 PDB header:transferase Chain: B: PDB Molecule:protein calg3; PDBTitle: crystal structure of calg3 from micromonospora echinospora determined2 in space group i222 28 c2aqmA_ not modelled 6.4 67 PDB header:oxidoreductase Chain: A: PDB Molecule:superoxide dismutase [cu-zn]; PDBTitle: cu/zn superoxide dismutase from brucella abortus 29 d2apsa_ not modelled 6.1 67 Fold:Immunoglobulin-like beta-sandwich Superfamily:Cu,Zn superoxide dismutase-like Family:Cu,Zn superoxide dismutase-like 30 d2rh3a1 not modelled 5.9 14 Fold:Ribbon-helix-helix Superfamily:Ribbon-helix-helix Family:VirC2-like 31 c1qupA_ not modelled 5.7 17 PDB header:chaperone Chain: A: PDB Molecule:superoxide dismutase 1 copper chaperone; PDBTitle: crystal structure of the copper chaperone for superoxide2 dismutase 32 c1jk9D_ not modelled 5.7 17 PDB header:oxidoreductase Chain: D: PDB Molecule:copper chaperone for superoxide dismutase; PDBTitle: heterodimer between h48f-ysod1 and yccs 33 d1ej8a_ not modelled 5.3 17 Fold:Immunoglobulin-like beta-sandwich Superfamily:Cu,Zn superoxide dismutase-like Family:Cu,Zn superoxide dismutase-like

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0