Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	S	Q	T	S	T	L	K	G	Q	C	I	A	E	F	L	G	T	G	L	L	I	F	F	G	V	G	C	V	A	A	L	K	V	A	G	A	S	F	G	Q	W	E	I	S	V	I	W	G	L	G	V	A	M	A	I	Y	L	T	A
Secondary structure
SS confidence
Disorder		?	?	?	?																													?	?					?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		G	V	S	G	A	H	L	N	P	A	V	T	I	A	L	W	L	F	A	C	F	D	K	R	K	V	I	P	F	I	V	S	Q	V	A	G	A	F	C	A	A	A	L	V	Y	G	L	Y	Y	N	L	F	F	D	F	E	Q	T	H	H
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		I	V	R	G	S	V	E	S	V	D	L	A	G	T	F	S	T	Y	P	N	P	H	I	N	F	V	Q	A	F	A	V	E	M	V	I	T	A	I	L	M	G	L	I	L	A	L	T	D	D	G	N	G	V	P	R	G	P	L	A	P
Secondary structure
SS confidence
Disorder																					?																													?	?		?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		L	L	I	G	L	L	I	A	V	I	G	A	S	M	G	P	L	T	G	F	A	M	N	P	A	R	D	F	G	P	K	V	F	A	W	L	A	G	W	G	N	V	A	F	T	G	G	R	D	I	P	Y	F	L	V	P	L	F	G	P
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.
Sequence		I	V	G	A	I	V	G	A	F	A	Y	R	K	L	I	G	R	H	L	P	C	D	I	C	V	V	E	E	K	E	T	T	T	P	S	E	Q	K	A	S	L
Secondary structure
SS confidence
Disorder																					?	?		?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 1lda chain A Region: 6 - 259 Aligned: 254 Modelled: 254 Confidence: 100.0% Identity: 100% PDB header:transport protein Chain: A: PDB Molecule:glycerol uptake facilitator protein; PDBTitle: crystal structure of the e. coli glycerol facilitator (glpf) without2 substrate glycerol Phyre2 PDB 1fx8 chain A Region: 6 - 259 Aligned: 254 Modelled: 254 Confidence: 100.0% Identity: 100% Fold: Aquaporin-like Superfamily: Aquaporin-like Family: Aquaporin-like Phyre2 PDB 3c02 chain A Region: 6 - 260 Aligned: 239 Modelled: 242 Confidence: 100.0% Identity: 36% PDB header:membrane protein Chain: A: PDB Molecule:aquaglyceroporin; PDBTitle: x-ray structure of the aquaglyceroporin from plasmodium falciparum Phyre2 PDB 2f2b chain A Region: 6 - 255 Aligned: 225 Modelled: 234 Confidence: 100.0% Identity: 36% PDB header:membrane protein Chain: A: PDB Molecule:aquaporin aqpm; PDBTitle: crystal structure of integral membrane protein aquaporin aqpm at 1.68a2 resolution Phyre2 PDB 2w2e chain A Region: 5 - 264 Aligned: 225 Modelled: 239 Confidence: 100.0% Identity: 27% PDB header:membrane protein Chain: A: PDB Molecule:aquaporin; PDBTitle: 1.15 angstrom crystal structure of p.pastoris aquaporin,2 aqy1, in a closed conformation at ph 3.5 Phyre2 PDB 1j4n chain A Region: 3 - 268 Aligned: 234 Modelled: 235 Confidence: 100.0% Identity: 29% Fold: Aquaporin-like Superfamily: Aquaporin-like Family: Aquaporin-like Phyre2 PDB 3llq chain B Region: 3 - 256 Aligned: 229 Modelled: 243 Confidence: 100.0% Identity: 30% PDB header:membrane protein Chain: B: PDB Molecule:aquaporin z 2; PDBTitle: aquaporin structure from plant pathogen agrobacterium tumerfaciens Phyre2 PDB 1ymg chain A Region: 6 - 267 Aligned: 227 Modelled: 244 Confidence: 100.0% Identity: 27% PDB header:membrane protein Chain: A: PDB Molecule:lens fiber major intrinsic protein; PDBTitle: the channel architecture of aquaporin o at 2.2 angstrom resolution Phyre2 PDB 1ymg chain A domain 1 Region: 6 - 267 Aligned: 227 Modelled: 240 Confidence: 100.0% Identity: 27% Fold: Aquaporin-like Superfamily: Aquaporin-like Family: Aquaporin-like Phyre2 PDB 2d57 chain A Region: 4 - 257 Aligned: 222 Modelled: 236 Confidence: 100.0% Identity: 28% PDB header:transport protein Chain: A: PDB Molecule:aquaporin-4; PDBTitle: double layered 2d crystal structure of aquaporin-4 (aqp4m23) at 3.2 a2 resolution by electron crystallography Phyre2 PDB 3d9s chain B Region: 3 - 274 Aligned: 239 Modelled: 254 Confidence: 100.0% Identity: 31% PDB header:membrane protein Chain: B: PDB Molecule:aquaporin-5; PDBTitle: human aquaporin 5 (aqp5) - high resolution x-ray structure Phyre2 PDB 3gd8 chain A Region: 5 - 257 Aligned: 221 Modelled: 235 Confidence: 100.0% Identity: 27% PDB header:membrane protein Chain: A: PDB Molecule:aquaporin-4; PDBTitle: crystal structure of human aquaporin 4 at 1.8 and its mechanism of2 conductance Phyre2 PDB 1h6i chain A Region: 7 - 257 Aligned: 219 Modelled: 233 Confidence: 100.0% Identity: 30% Fold: Aquaporin-like Superfamily: Aquaporin-like Family: Aquaporin-like Phyre2 PDB 1rc2 chain A Region: 7 - 259 Aligned: 230 Modelled: 253 Confidence: 100.0% Identity: 29% Fold: Aquaporin-like Superfamily: Aquaporin-like Family: Aquaporin-like Phyre2 PDB 3iyz chain A Region: 4 - 255 Aligned: 220 Modelled: 234 Confidence: 100.0% Identity: 28% PDB header:transport protein Chain: A: PDB Molecule:aquaporin-4; PDBTitle: structure of aquaporin-4 s180d mutant at 10.0 a resolution from2 electron micrograph Phyre2 PDB 2b5f chain D Region: 2 - 254 Aligned: 223 Modelled: 236 Confidence: 100.0% Identity: 29% PDB header:transport protein,membrane protein Chain: D: PDB Molecule:aquaporin; PDBTitle: crystal structure of the spinach aquaporin sopip2;1 in an2 open conformation to 3.9 resolution Phyre2 PDB 3kcv chain G Region: 5 - 257 Aligned: 230 Modelled: 253 Confidence: 72.7% Identity: 12% PDB header:transport protein Chain: G: PDB Molecule:probable formate transporter 1; PDBTitle: structure of formate channel Phyre2 PDB 2knc chain A Region: 233 - 270 Aligned: 38 Modelled: 38 Confidence: 58.1% Identity: 24% PDB header:cell adhesion Chain: A: PDB Molecule:integrin alpha-iib; PDBTitle: platelet integrin alfaiib-beta3 transmembrane-cytoplasmic2 heterocomplex Phyre2 PDB 1f0k chain A Region: 63 - 80 Aligned: 18 Modelled: 18 Confidence: 33.6% Identity: 33% Fold: UDP-Glycosyltransferase/glycogen phosphorylase Superfamily: UDP-Glycosyltransferase/glycogen phosphorylase Family: Peptidoglycan biosynthesis glycosyltransferase MurG Phyre2 PDB 3qnq chain D Region: 178 - 274 Aligned: 86 Modelled: 97 Confidence: 27.6% Identity: 12% PDB header:membrane protein, transport protein Chain: D: PDB Molecule:pts system, cellobiose-specific iic component; PDBTitle: crystal structure of the transporter chbc, the iic component from the2 n,n'-diacetylchitobiose-specific phosphotransferase system Phyre2 1 c1ldaA_ 2 d1fx8a_ 3 c3c02A_ 4 c2f2bA_ 5 c2w2eA_ 6 d1j4na_ 7 c3llqB_ 8 c1ymgA_ 9 d1ymga1 10 c2d57A_ 11 c3d9sB_ 12 c3gd8A_ 13 d1h6ia_ 14 d1rc2a_ 15 c3iyzA_ 16 c2b5fD_ 17 c3kcvG_ 18 c2kncA_ 19 d1f0ka_ 20 c3qnqD_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 c1ldaA_ 100.0 100 PDB header:transport protein Chain: A: PDB Molecule:glycerol uptake facilitator protein; PDBTitle: crystal structure of the e. coli glycerol facilitator (glpf) without2 substrate glycerol 2 d1fx8a_ 100.0 100 Fold:Aquaporin-like Superfamily:Aquaporin-like Family:Aquaporin-like 3 c3c02A_ 100.0 36 PDB header:membrane protein Chain: A: PDB Molecule:aquaglyceroporin; PDBTitle: x-ray structure of the aquaglyceroporin from plasmodium falciparum 4 c2f2bA_ 100.0 36 PDB header:membrane protein Chain: A: PDB Molecule:aquaporin aqpm; PDBTitle: crystal structure of integral membrane protein aquaporin aqpm at 1.68a2 resolution 5 c2w2eA_ 100.0 27 PDB header:membrane protein Chain: A: PDB Molecule:aquaporin; PDBTitle: 1.15 angstrom crystal structure of p.pastoris aquaporin,2 aqy1, in a closed conformation at ph 3.5 6 d1j4na_ 100.0 29 Fold:Aquaporin-like Superfamily:Aquaporin-like Family:Aquaporin-like 7 c3llqB_ 100.0 30 PDB header:membrane protein Chain: B: PDB Molecule:aquaporin z 2; PDBTitle: aquaporin structure from plant pathogen agrobacterium tumerfaciens 8 c1ymgA_ 100.0 27 PDB header:membrane protein Chain: A: PDB Molecule:lens fiber major intrinsic protein; PDBTitle: the channel architecture of aquaporin o at 2.2 angstrom resolution 9 d1ymga1 100.0 27 Fold:Aquaporin-like Superfamily:Aquaporin-like Family:Aquaporin-like 10 c2d57A_ 100.0 28 PDB header:transport protein Chain: A: PDB Molecule:aquaporin-4; PDBTitle: double layered 2d crystal structure of aquaporin-4 (aqp4m23) at 3.2 a2 resolution by electron crystallography 11 c3d9sB_ 100.0 31 PDB header:membrane protein Chain: B: PDB Molecule:aquaporin-5; PDBTitle: human aquaporin 5 (aqp5) - high resolution x-ray structure 12 c3gd8A_ 100.0 27 PDB header:membrane protein Chain: A: PDB Molecule:aquaporin-4; PDBTitle: crystal structure of human aquaporin 4 at 1.8 and its mechanism of2 conductance 13 d1h6ia_ 100.0 30 Fold:Aquaporin-like Superfamily:Aquaporin-like Family:Aquaporin-like 14 d1rc2a_ 100.0 29 Fold:Aquaporin-like Superfamily:Aquaporin-like Family:Aquaporin-like 15 c3iyzA_ 100.0 28 PDB header:transport protein Chain: A: PDB Molecule:aquaporin-4; PDBTitle: structure of aquaporin-4 s180d mutant at 10.0 a resolution from2 electron micrograph 16 c2b5fD_ 100.0 29 PDB header:transport protein,membrane protein Chain: D: PDB Molecule:aquaporin; PDBTitle: crystal structure of the spinach aquaporin sopip2;1 in an2 open conformation to 3.9 resolution 17 c3kcvG_ 72.7 12 PDB header:transport protein Chain: G: PDB Molecule:probable formate transporter 1; PDBTitle: structure of formate channel 18 c2kncA_ 58.1 24 PDB header:cell adhesion Chain: A: PDB Molecule:integrin alpha-iib; PDBTitle: platelet integrin alfaiib-beta3 transmembrane-cytoplasmic2 heterocomplex 19 d1f0ka_ 33.6 33 Fold:UDP-Glycosyltransferase/glycogen phosphorylase Superfamily:UDP-Glycosyltransferase/glycogen phosphorylase Family:Peptidoglycan biosynthesis glycosyltransferase MurG 20 c3qnqD_ 27.6 12 PDB header:membrane protein, transport protein Chain: D: PDB Molecule:pts system, cellobiose-specific iic component; PDBTitle: crystal structure of the transporter chbc, the iic component from the2 n,n'-diacetylchitobiose-specific phosphotransferase system 21 d1pn3a_ not modelled 21.5 28 Fold:UDP-Glycosyltransferase/glycogen phosphorylase Superfamily:UDP-Glycosyltransferase/glycogen phosphorylase Family:Gtf glycosyltransferase 22 d1rrva_ not modelled 19.2 22 Fold:UDP-Glycosyltransferase/glycogen phosphorylase Superfamily:UDP-Glycosyltransferase/glycogen phosphorylase Family:Gtf glycosyltransferase 23 d1iira_ not modelled 18.5 22 Fold:UDP-Glycosyltransferase/glycogen phosphorylase Superfamily:UDP-Glycosyltransferase/glycogen phosphorylase Family:Gtf glycosyltransferase 24 c3orgB_ not modelled 11.6 11 PDB header:transport protein Chain: B: PDB Molecule:cmclc; PDBTitle: crystal structure of a eukaryotic clc transporter 25 c1s4iC_ not modelled 9.4 43 PDB header:oxidoreductase Chain: C: PDB Molecule:superoxide dismutase-like protein yojm; PDBTitle: crystal structure of a sod-like protein from bacillus subtilis 26 c3d0qB_ not modelled 9.2 22 PDB header:transferase Chain: B: PDB Molecule:protein calg3; PDBTitle: crystal structure of calg3 from micromonospora echinospora determined2 in space group i222 27 d1esoa_ not modelled 8.3 67 Fold:Immunoglobulin-like beta-sandwich Superfamily:Cu,Zn superoxide dismutase-like Family:Cu,Zn superoxide dismutase-like 28 d1eqwa_ not modelled 8.1 57 Fold:Immunoglobulin-like beta-sandwich Superfamily:Cu,Zn superoxide dismutase-like Family:Cu,Zn superoxide dismutase-like 29 d1oala_ not modelled 7.4 50 Fold:Immunoglobulin-like beta-sandwich Superfamily:Cu,Zn superoxide dismutase-like Family:Cu,Zn superoxide dismutase-like 30 c2aqmA_ not modelled 7.2 50 PDB header:oxidoreductase Chain: A: PDB Molecule:superoxide dismutase [cu-zn]; PDBTitle: cu/zn superoxide dismutase from brucella abortus 31 d2apsa_ not modelled 6.9 50 Fold:Immunoglobulin-like beta-sandwich Superfamily:Cu,Zn superoxide dismutase-like Family:Cu,Zn superoxide dismutase-like 32 d1yrra2 not modelled 6.5 40 Fold:TIM beta/alpha-barrel Superfamily:Metallo-dependent hydrolases Family:N-acetylglucosamine-6-phosphate deacetylase, NagA, catalytic domain 33 c1qupA_ not modelled 6.2 17 PDB header:chaperone Chain: A: PDB Molecule:superoxide dismutase 1 copper chaperone; PDBTitle: crystal structure of the copper chaperone for superoxide2 dismutase 34 c1jk9D_ not modelled 6.2 17 PDB header:oxidoreductase Chain: D: PDB Molecule:copper chaperone for superoxide dismutase; PDBTitle: heterodimer between h48f-ysod1 and yccs 35 d1f1ga_ not modelled 6.0 57 Fold:Immunoglobulin-like beta-sandwich Superfamily:Cu,Zn superoxide dismutase-like Family:Cu,Zn superoxide dismutase-like 36 d1to4a_ not modelled 6.0 71 Fold:Immunoglobulin-like beta-sandwich Superfamily:Cu,Zn superoxide dismutase-like Family:Cu,Zn superoxide dismutase-like 37 d1un7a2 not modelled 5.9 30 Fold:TIM beta/alpha-barrel Superfamily:Metallo-dependent hydrolases Family:N-acetylglucosamine-6-phosphate deacetylase, NagA, catalytic domain 38 d2c9va1 not modelled 5.8 57 Fold:Immunoglobulin-like beta-sandwich Superfamily:Cu,Zn superoxide dismutase-like Family:Cu,Zn superoxide dismutase-like 39 d1ej8a_ not modelled 5.8 17 Fold:Immunoglobulin-like beta-sandwich Superfamily:Cu,Zn superoxide dismutase-like Family:Cu,Zn superoxide dismutase-like 40 c2qvtA_ not modelled 5.8 38 PDB header:unknown function Chain: A: PDB Molecule:avrl567-d; PDBTitle: structure of melampsora lini avirulence protein, avrl567-d 41 d1jk9b1 not modelled 5.8 17 Fold:Immunoglobulin-like beta-sandwich Superfamily:Cu,Zn superoxide dismutase-like Family:Cu,Zn superoxide dismutase-like 42 c2q2lB_ not modelled 5.7 57 PDB header:oxidoreductase Chain: B: PDB Molecule:superoxide dismutase; PDBTitle: crystal structure of superoxide dismutase from p.2 atrosanguina 43 c2jlpA_ not modelled 5.6 67 PDB header:oxidoreductase Chain: A: PDB Molecule:extracellular superoxide dismutase (cu-zn); PDBTitle: crystal structure of human extracellular copper-zinc2 superoxide dismutase. 44 d1do5a_ not modelled 5.6 57 Fold:Immunoglobulin-like beta-sandwich Superfamily:Cu,Zn superoxide dismutase-like Family:Cu,Zn superoxide dismutase-like 45 c3ia7A_ not modelled 5.4 17 PDB header:transferase Chain: A: PDB Molecule:calg4; PDBTitle: crystal structure of calg4, the calicheamicin glycosyltransferase 46 c3klzE_ not modelled 5.3 14 PDB header:membrane protein Chain: E: PDB Molecule:putative formate transporter 1; PDBTitle: pentameric formate channel with formate bound 47 c3iaaB_ not modelled 5.3 22 PDB header:transferase Chain: B: PDB Molecule:calg2; PDBTitle: crystal structure of calg2, calicheamicin glycosyltransferase, tdp2 bound form

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

Transmembrane helix prediction

Transmembrane helices have been predicted in your sequence to adopt the topology shown below

---

Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0