Phyre Home Retrieve Phyre Job Id
Emaill.a.kelley@imperial.ac.uk
DescriptionP39336
DateThu Jan 5 11:59:27 GMT 2012
Unique Job IDf4d7d81ed7f3e669

Summary 

Top model
Image coloured by rainbow N → C terminus
Model (left) based on template c2qzaA_
Top template information
PDB header:ligase
Chain: A: PDB Molecule:secreted effector protein;
PDBTitle: crystal structure of salmonella effector protein sopa
Confidence and coverage
Confidence: 95.3% Coverage: 12%
73 residues ( 12% of your sequence) have been modelled with 95.3% confidence by the single highest scoring template.
You may wish to submit your sequence to Phyrealarm. This will automatically scan your sequence every week for new potential templates as they appear in the Phyre2 library.
3D viewing
Interactive 3D view in Jmol

Sequence analysis 

Secondary structure and disorder prediction 

   1........10.........20.........30.........40.........50.........60
Sequence  MSKISDLNYSQHITLADNFKQKSEVLNTWRVGMNDFARIAGGQDNRRNILSPGAFLEFLA
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   .........70.........80.........90.........100.........110.........120
Sequence  KIFTLGYVDFSKRSNEAGRNMMAHIKSSSYSKDTNGNEKMKFYMNNPVGERADSPKVIIE
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   .........130.........140.........150.........160.........170.........180
Sequence  ISLSTITTMGTRQGHTAIIFPQPDGSTNRYEGKSFERKDESSLHLITNKVLACYQSEANK
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   .........190.........200.........210.........220.........230.........240
Sequence  KIARLLNNNQELNNLQKLNNLQKLNNLLKLNNIQGLNNPQELNNPQNLNDSQELNNSQEL
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Disorder  ????????????????????????????????????????????????????????????
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   .........250.........260.........270.........280.........290.........300
Sequence  NSPQELNDPQELNNSQDLNNSKVSCTVSVDSTITGLLKEPLNNALLAIRNEHLLLMPHVC
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   .........310.........320.........330.........340.........350.........360
Sequence  DESISYLLGEKGILEEIDKLYALNDHGIDNDKVGNNEINDIKVNLSHILIDSLDDAKVNL
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   .........370.........380.........390.........400.........410.........420
Sequence  TPVIDSILETFSKSPYINDVRILDWCFNKSMQYFDDTKKIKHACSVINHINLRSDQSKIA
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   .........430.........440.........450.........460.........470.........480
Sequence  ETLFFNLDKEPYKNSPELQGLIWNKLVVYVNEFNLSNREKTNLIQRLFDNVESIFNEVPV
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   .........490.........500.........510.........520.........530.........540
Sequence  SILVNDIFMNDFFMKNPEMINWYFPQLLKSYEGEKIYFDNLKYDLNDNDKESNKEILKNQ
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   .........550.........560.........570.........580.........590.........600
Sequence  PDNVIKEKLNNEYKLRFRMMQTILQSRVNVLPYINEQRLNKLNPPENLRIAIEHFGWKNR
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   ....
Sequence  PITA
Secondary structure 



SS confidence 



Disorder  ????
Disorder confidence 



 

Confidence Key
High(9)                    Low (0)
?Disordered
Alpha helix
Beta strand

Domain analysis 

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

RankAligned region

PDB 2qza chain A

3D model

Region: 310 - 396
Aligned: 73
Modelled: 73
Confidence: 95.3%
Identity: 27%
PDB header:ligase
Chain: A: PDB Molecule:secreted effector protein;
PDBTitle: crystal structure of salmonella effector protein sopa

Phyre2

PDB 3nb2 chain B

3D model

Region: 311 - 396
Aligned: 72
Modelled: 72
Confidence: 94.6%
Identity: 31%
PDB header:ligase
Chain: B: PDB Molecule:secreted effector protein;
PDBTitle: crystal structure of e. coli o157:h7 effector protein nlel

Phyre2

PDB 1qcs chain A domain 2

3D model

Region: 98 - 154
Aligned: 52
Modelled: 57
Confidence: 24.9%
Identity: 21%
Fold: Cdc48 domain 2-like
Superfamily: Cdc48 domain 2-like
Family: Cdc48 domain 2-like

Phyre2

PDB 1vmj chain A

3D model

Region: 55 - 88
Aligned: 34
Modelled: 34
Confidence: 19.3%
Identity: 24%
Fold: YjbQ-like
Superfamily: YjbQ-like
Family: YjbQ-like

Phyre2

PDB 1ve0 chain A

3D model

Region: 55 - 88
Aligned: 31
Modelled: 34
Confidence: 18.3%
Identity: 29%
PDB header:metal binding protein
Chain: A: PDB Molecule:hypothetical protein (st2072);
PDBTitle: crystal structure of uncharacterized protein st2072 from sulfolobus2 tokodaii

Phyre2

PDB 1vph chain A

3D model

Region: 51 - 88
Aligned: 38
Modelled: 38
Confidence: 18.0%
Identity: 24%
Fold: YjbQ-like
Superfamily: YjbQ-like
Family: YjbQ-like

Phyre2

PDB 2w8d chain B

3D model

Region: 318 - 369
Aligned: 52
Modelled: 52
Confidence: 17.4%
Identity: 17%
PDB header:transferase
Chain: B: PDB Molecule:processed glycerol phosphate lipoteichoic acid synthase 2;
PDBTitle: distinct and essential morphogenic functions for wall- and2 lipo-teichoic acids in bacillus subtilis

Phyre2

PDB 3ik5 chain A

3D model

Region: 296 - 328
Aligned: 33
Modelled: 33
Confidence: 16.9%
Identity: 33%
PDB header:viral protein/signaling protein
Chain: A: PDB Molecule:protein nef;
PDBTitle: sivmac239 nef in complex with tcr zeta itam 1 polypeptide2 (a63-r80)

Phyre2

PDB 1s4k chain A

3D model

Region: 388 - 429
Aligned: 42
Modelled: 42
Confidence: 16.6%
Identity: 24%
Fold: lambda repressor-like DNA-binding domains
Superfamily: lambda repressor-like DNA-binding domains
Family: YdiL-like

Phyre2

PDB 2p6c chain B

3D model

Region: 55 - 88
Aligned: 32
Modelled: 34
Confidence: 15.9%
Identity: 28%
PDB header:structural genomics, unknown function
Chain: B: PDB Molecule:aq_2013 protein;
PDBTitle: crystal structure of hypothetical protein aq_2013 from aquifex2 aeolicus vf5.

Phyre2

PDB 2py5 chain A domain 1

3D model

Region: 498 - 524
Aligned: 21
Modelled: 27
Confidence: 14.9%
Identity: 33%
Fold: Ribonuclease H-like motif
Superfamily: Ribonuclease H-like
Family: DnaQ-like 3'-5' exonuclease

Phyre2

PDB 2jyn chain A

3D model

Region: 349 - 373
Aligned: 25
Modelled: 25
Confidence: 14.2%
Identity: 36%
PDB header:structural genomics, unknown function
Chain: A: PDB Molecule:upf0368 protein ypl225w;
PDBTitle: a novel solution nmr structure of protein yst0336 from2 saccharomyces cerevisiae. northeast structural genomics3 consortium target yt51/ontario centre for structural4 proteomics target yst0336

Phyre2

PDB 2xi1 chain A

3D model

Region: 296 - 328
Aligned: 33
Modelled: 33
Confidence: 14.2%
Identity: 39%
PDB header:viral protein
Chain: A: PDB Molecule:nef;
PDBTitle: crystal structure of the hiv-1 nef sequenced from a patient's sample

Phyre2

PDB 1efn chain B

3D model

Region: 296 - 328
Aligned: 33
Modelled: 33
Confidence: 13.4%
Identity: 33%
Fold: Regulatory factor Nef
Superfamily: Regulatory factor Nef
Family: Regulatory factor Nef

Phyre2

PDB 3rbb chain A

3D model

Region: 296 - 328
Aligned: 33
Modelled: 33
Confidence: 12.4%
Identity: 39%
PDB header:viral protein, protein binding
Chain: A: PDB Molecule:protein nef;
PDBTitle: hiv-1 nef protein in complex with engineered hck sh3 domain

Phyre2

PDB 2nef chain A

3D model

Region: 296 - 328
Aligned: 33
Modelled: 33
Confidence: 12.1%
Identity: 33%
Fold: Regulatory factor Nef
Superfamily: Regulatory factor Nef
Family: Regulatory factor Nef

Phyre2

PDB 3ebc chain A

3D model

Region: 488 - 500
Aligned: 13
Modelled: 13
Confidence: 11.9%
Identity: 62%
PDB header:hydrolase/dna
Chain: A: PDB Molecule:type-2 restriction enzyme hincii;
PDBTitle: structure of n141a hincii with cognate dna

Phyre2

PDB 1bf4 chain A

3D model

Region: 29 - 61
Aligned: 33
Modelled: 33
Confidence: 11.8%
Identity: 42%
Fold: SH3-like barrel
Superfamily: Chromo domain-like
Family: "Histone-like" proteins from archaea

Phyre2

PDB 1p82 chain A

3D model

Region: 356 - 368
Aligned: 13
Modelled: 13
Confidence: 11.6%
Identity: 62%
PDB header:chaperone
Chain: A: PDB Molecule:10 kda chaperonin;
PDBTitle: nmr structure of 1-25 fragment of mycobacterium2 tuberculosis cpn10

Phyre2

PDB 2pgc chain A domain 1

3D model

Region: 62 - 93
Aligned: 25
Modelled: 32
Confidence: 11.5%
Identity: 56%
Fold: Ferredoxin-like
Superfamily: Dimeric alpha+beta barrel
Family: Marine metagenome family DABB3

Phyre2
1

c2qzaA_
2

c3nb2B_
3

d1qcsa2
4

d1vmja_
5

c1ve0A_
6

d1vpha_
7

c2w8dB_
8

c3ik5A_
9

d1s4ka_
10

c2p6cB_
11

d2py5a1
12

c2jynA_
13

c2xi1A_
14

d1efnb_
15

c3rbbA_
16

d2nefa_
17

c3ebcA_
18

d1bf4a_
19

c1p82A_
20

d2pgca1
21



22



23



24



25



26



27



28



29



30



31



32



33



34



35



36



37



38



39



40



41



42



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46



47



48



49



50



51



52



53



54



55



56






Detailed template information 

#
Template Alignment Coverage3D Model Confidence
% i.d. Template Information
1c2qzaA_



95.3 27 PDB header:ligase
Chain: A: PDB Molecule:secreted effector protein;
PDBTitle: crystal structure of salmonella effector protein sopa
2c3nb2B_



94.6 31 PDB header:ligase
Chain: B: PDB Molecule:secreted effector protein;
PDBTitle: crystal structure of e. coli o157:h7 effector protein nlel
3d1qcsa2



24.9 21 Fold:Cdc48 domain 2-like
Superfamily:Cdc48 domain 2-like
Family:Cdc48 domain 2-like
4d1vmja_



19.3 24 Fold:YjbQ-like
Superfamily:YjbQ-like
Family:YjbQ-like
5c1ve0A_



18.3 29 PDB header:metal binding protein
Chain: A: PDB Molecule:hypothetical protein (st2072);
PDBTitle: crystal structure of uncharacterized protein st2072 from sulfolobus2 tokodaii
6d1vpha_



18.0 24 Fold:YjbQ-like
Superfamily:YjbQ-like
Family:YjbQ-like
7c2w8dB_



17.4 17 PDB header:transferase
Chain: B: PDB Molecule:processed glycerol phosphate lipoteichoic acid synthase 2;
PDBTitle: distinct and essential morphogenic functions for wall- and2 lipo-teichoic acids in bacillus subtilis
8c3ik5A_



16.9 33 PDB header:viral protein/signaling protein
Chain: A: PDB Molecule:protein nef;
PDBTitle: sivmac239 nef in complex with tcr zeta itam 1 polypeptide2 (a63-r80)
9d1s4ka_



16.6 24 Fold:lambda repressor-like DNA-binding domains
Superfamily:lambda repressor-like DNA-binding domains
Family:YdiL-like
10c2p6cB_



15.9 28 PDB header:structural genomics, unknown function
Chain: B: PDB Molecule:aq_2013 protein;
PDBTitle: crystal structure of hypothetical protein aq_2013 from aquifex2 aeolicus vf5.
11d2py5a1



14.9 33 Fold:Ribonuclease H-like motif
Superfamily:Ribonuclease H-like
Family:DnaQ-like 3'-5' exonuclease
12c2jynA_



14.2 36 PDB header:structural genomics, unknown function
Chain: A: PDB Molecule:upf0368 protein ypl225w;
PDBTitle: a novel solution nmr structure of protein yst0336 from2 saccharomyces cerevisiae. northeast structural genomics3 consortium target yt51/ontario centre for structural4 proteomics target yst0336
13c2xi1A_



14.2 39 PDB header:viral protein
Chain: A: PDB Molecule:nef;
PDBTitle: crystal structure of the hiv-1 nef sequenced from a patient's sample
14d1efnb_



13.4 33 Fold:Regulatory factor Nef
Superfamily:Regulatory factor Nef
Family:Regulatory factor Nef
15c3rbbA_



12.4 39 PDB header:viral protein, protein binding
Chain: A: PDB Molecule:protein nef;
PDBTitle: hiv-1 nef protein in complex with engineered hck sh3 domain
16d2nefa_



12.1 33 Fold:Regulatory factor Nef
Superfamily:Regulatory factor Nef
Family:Regulatory factor Nef
17c3ebcA_



11.9 62 PDB header:hydrolase/dna
Chain: A: PDB Molecule:type-2 restriction enzyme hincii;
PDBTitle: structure of n141a hincii with cognate dna
18d1bf4a_



11.8 42 Fold:SH3-like barrel
Superfamily:Chromo domain-like
Family:"Histone-like" proteins from archaea
19c1p82A_



11.6 62 PDB header:chaperone
Chain: A: PDB Molecule:10 kda chaperonin;
PDBTitle: nmr structure of 1-25 fragment of mycobacterium2 tuberculosis cpn10
20d2pgca1



11.5 56 Fold:Ferredoxin-like
Superfamily:Dimeric alpha+beta barrel
Family:Marine metagenome family DABB3
21d1tx3a1



not modelled 11.3 62 Fold:Restriction endonuclease-like
Superfamily:Restriction endonuclease-like
Family:Restriction endonuclease HincII
22d1vj0a1



not modelled 10.9 20 Fold:GroES-like
Superfamily:GroES-like
Family:Alcohol dehydrogenase-like, N-terminal domain
23c2xrhA_



not modelled 10.8 32 PDB header:unknown function
Chain: A: PDB Molecule:protein hp0721;
PDBTitle: crystal structure of the truncated form of hp0721
24c2elpA_



not modelled 9.0 55 PDB header:transcription
Chain: A: PDB Molecule:zinc finger protein 406;
PDBTitle: solution structure of the 13th c2h2 zinc finger of human2 zinc finger protein 406
25d1alqa_



not modelled 8.9 22 Fold:beta-lactamase/transpeptidase-like
Superfamily:beta-lactamase/transpeptidase-like
Family:beta-Lactamase/D-ala carboxypeptidase
26d1ug7a_



not modelled 8.6 20 Fold:Four-helical up-and-down bundle
Superfamily:Domain from hypothetical 2610208m17rik protein
Family:Domain from hypothetical 2610208m17rik protein
27c2qyxB_



not modelled 8.3 31 PDB header:structural genomics, unknown function
Chain: B: PDB Molecule:uncharacterized protein mj0159;
PDBTitle: crystal structure of uncharacterized protein mj0159 from2 methanocaldococcus jannaschii
28c2jvfA_



not modelled 8.1 32 PDB header:de novo protein
Chain: A: PDB Molecule:de novo protein m7;
PDBTitle: solution structure of m7, a computationally-designed2 artificial protein
29c1qysA_



not modelled 7.9 29 PDB header:de novo protein
Chain: A: PDB Molecule:top7;
PDBTitle: crystal structure of top7: a computationally designed2 protein with a novel fold
30c2ejsA_



not modelled 7.9 17 PDB header:ligase
Chain: A: PDB Molecule:autocrine motility factor receptor, isoform 2;
PDBTitle: solution structure of ruh-076, a human cue domain
31d1y14a_



not modelled 7.8 12 Fold:SAM domain-like
Superfamily:HRDC-like
Family:RNA polymerase II subunit RBP4 (RpoF)
32c2r8rB_



not modelled 7.7 26 PDB header:transferase
Chain: B: PDB Molecule:sensor protein;
PDBTitle: crystal structure of the n-terminal region (19..243) of sensor protein2 kdpd from pseudomonas syringae pv. tomato str. dc3000
33c3lgeG_



not modelled 7.3 47 PDB header:lyase/protein binding
Chain: G: PDB Molecule:sorting nexin-9;
PDBTitle: crystal structure of rabbit muscle aldolase-snx9 lc4 complex
34c3lgeF_



not modelled 7.3 47 PDB header:lyase/protein binding
Chain: F: PDB Molecule:sorting nexin-9;
PDBTitle: crystal structure of rabbit muscle aldolase-snx9 lc4 complex
35c3h0gP_



not modelled 6.9 21 PDB header:transcription
Chain: P: PDB Molecule:dna-directed rna polymerase ii subunit rpb4;
PDBTitle: rna polymerase ii from schizosaccharomyces pombe
36c3l76B_



not modelled 6.7 42 PDB header:transferase
Chain: B: PDB Molecule:aspartokinase;
PDBTitle: crystal structure of aspartate kinase from synechocystis
37c1qdnA_



not modelled 6.6 21 PDB header:fusion protein
Chain: A: PDB Molecule:protein (n-ethylmaleimide sensitive fusion
PDBTitle: amino terminal domain of the n-ethylmaleimide sensitive2 fusion protein (nsf)
38c2klqA_



not modelled 6.5 29 PDB header:replication
Chain: A: PDB Molecule:dna replication licensing factor mcm6;
PDBTitle: the solution structure of cbd of human mcm6
39d1vmfa_



not modelled 6.1 23 Fold:YjbQ-like
Superfamily:YjbQ-like
Family:YjbQ-like
40d2qrdb1



not modelled 6.0 44 Fold:AMPKBI-like
Superfamily:AMPKBI-like
Family:AMPKBI-like
41d1p2fa1



not modelled 5.9 16 Fold:DNA/RNA-binding 3-helical bundle
Superfamily:C-terminal effector domain of the bipartite response regulators
Family:PhoB-like
42c3fo8D_



not modelled 5.9 43 PDB header:viral protein
Chain: D: PDB Molecule:tail sheath protein gp18;
PDBTitle: crystal structure of the bacteriophage t4 tail sheath2 protein, protease resistant fragment gp18pr
43c2ex3I_



not modelled 5.9 33 PDB header:transferase/replication
Chain: I: PDB Molecule:dna polymerase;
PDBTitle: bacteriophage phi29 dna polymerase bound to terminal protein
44c2wzpR_



not modelled 5.8 28 PDB header:viral protein
Chain: R: PDB Molecule:lactococcal phage p2 orf16;
PDBTitle: structures of lactococcal phage p2 baseplate shed light on2 a novel mechanism of host attachment and activation in3 siphoviridae
45c3gpkA_



not modelled 5.8 12 PDB header:isomerase
Chain: A: PDB Molecule:ppic-type peptidyl-prolyl cis-trans isomerase;
PDBTitle: crystal structure of ppic-type peptidyl-prolyl cis-trans isomerase2 domain at 1.55a resolution.
46c2k0mA_



not modelled 5.7 20 PDB header:structural genomics, unknown function
Chain: A: PDB Molecule:uncharacterized protein;
PDBTitle: solution nmr structure of the uncharacterized protein from2 rhodospirillum rubrum gene locus rru_a0810. northeast3 structural genomics target rrr43
47d1gado1



not modelled 5.6 58 Fold:NAD(P)-binding Rossmann-fold domains
Superfamily:NAD(P)-binding Rossmann-fold domains
Family:Glyceraldehyde-3-phosphate dehydrogenase-like, N-terminal domain
48d1ggaa1



not modelled 5.6 58 Fold:NAD(P)-binding Rossmann-fold domains
Superfamily:NAD(P)-binding Rossmann-fold domains
Family:Glyceraldehyde-3-phosphate dehydrogenase-like, N-terminal domain
49d2qlvb2



not modelled 5.5 44 Fold:AMPKBI-like
Superfamily:AMPKBI-like
Family:AMPKBI-like
50d2nn4a1



not modelled 5.4 37 Fold:YqgQ-like
Superfamily:YqgQ-like
Family:YqgQ-like
51c3jszA_



not modelled 5.4 35 PDB header:transferase
Chain: A: PDB Molecule:putative uncharacterized protein;
PDBTitle: legionella pneumophila glucosyltransferase lgt1 n293a with udp-glc
52d2v8qb1



not modelled 5.3 44 Fold:AMPKBI-like
Superfamily:AMPKBI-like
Family:AMPKBI-like
53c2qdjA_



not modelled 5.2 11 PDB header:antitumor protein
Chain: A: PDB Molecule:retinoblastoma-associated protein;
PDBTitle: crystal structure of the retinoblastoma protein n-domain2 provides insight into tumor suppression, ligand3 interaction and holoprotein architecture
54c2cp8A_



not modelled 5.2 31 PDB header:protein binding
Chain: A: PDB Molecule:next to brca1 gene 1 protein;
PDBTitle: solution structure of the rsgi ruh-046, a uba domain from2 human next to brca1 gene 1 protein (kiaa0049 protein)3 r923h variant
55c2cu5C_



not modelled 5.2 27 PDB header:structural genomics, unknown function
Chain: C: PDB Molecule:conserved hypothetical protein tt1486;
PDBTitle: crystal structure of the conserved hypothetical protein tt1486 from2 thermus thermophilus hb8
56c1zk6A_



not modelled 5.1 38 PDB header:isomerase
Chain: A: PDB Molecule:foldase protein prsa;
PDBTitle: nmr solution structure of b. subtilis prsa ppiase

Binding site prediction 

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

Transmembrane helix prediction 

Transmembrane helices have been predicted in your sequence to adopt the topology shown below



Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
 
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
 
© Structural Bioinformatics Group
Imperial College London
Lawrence Kelley, Benjamin Jefferys
Disclaimer
Terms and Conditions
Component software
Template detection: HHpred 1.51
Secondary structure prediction: Psi-pred 2.5
Disorder prediction: Disopred 2.4
Transmembrane prediction: Memsat_SVM
Multi-template modelling and ab initio: Poing 1.0