Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	S	T	D	N	K	Q	S	L	P	A	I	T	L	A	A	I	G	V	V	Y	G	D	I	G	T	S	P	L	Y	T	L	R	E	C	L	S	G	Q	F	G	F	G	V	E	R	D	A	V	F	G	F	L	S	L	I	F	W	L	L
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?																														?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		I	F	V	V	S	I	K	Y	L	T	F	V	M	R	A	D	N	A	G	E	G	G	I	L	T	L	M	S	L	A	G	R	N	T	S	A	R	T	T	S	M	L	V	I	M	G	L	I	G	G	S	F	F	Y	G	E	V	V	I	T
Secondary structure
SS confidence
Disorder																																		?	?	?	?	?	?																?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		P	A	I	S	V	M	S	A	I	E	G	L	E	I	V	A	P	Q	L	D	T	W	I	V	P	L	S	I	I	V	L	T	L	L	F	M	I	Q	K	H	G	T	A	M	V	G	K	L	F	A	P	I	M	L	T	W	F	L	I	L
Secondary structure
SS confidence
Disorder											?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		A	G	L	G	L	R	S	I	I	A	N	P	E	V	L	H	A	L	N	P	M	W	A	V	H	F	F	L	E	Y	K	T	V	S	F	I	A	L	G	A	V	V	L	S	I	T	G	V	E	A	L	Y	A	D	M	G	H	F	G	K
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		F	P	I	R	L	A	W	F	T	V	V	L	P	S	L	T	L	N	Y	F	G	Q	G	A	L	L	L	K	N	P	E	A	I	K	N	P	F	F	L	L	A	P	D	W	A	L	I	P	L	L	I	I	A	A	L	A	T	V	I	A
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		S	Q	A	V	I	S	G	V	F	S	L	T	R	Q	A	V	R	L	G	Y	L	S	P	M	R	I	I	H	T	S	E	M	E	S	G	Q	I	Y	I	P	F	V	N	W	M	L	Y	V	A	V	V	I	V	I	V	S	F	E	H	S
Secondary structure
SS confidence
Disorder																				?								?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420
Sequence		S	N	L	A	A	A	Y	G	I	A	V	T	G	T	M	V	L	T	S	I	L	S	T	T	V	A	R	Q	N	W	H	W	N	K	Y	F	V	A	L	I	L	I	A	F	L	C	V	D	I	P	L	F	T	A	N	L	D	K	L	L
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.	.	.	.	.	.	.	460	.	.	.	.	.	.	.	.	.	470	.	.	.	.	.	.	.	.	.	480
Sequence		S	G	G	W	L	P	L	S	L	G	T	V	M	F	I	V	M	T	T	W	K	S	E	R	F	R	L	L	R	R	M	H	E	H	G	N	S	L	E	A	M	I	A	S	L	E	K	S	P	P	V	R	V	P	G	T	A	V	Y	M
Secondary structure
SS confidence
Disorder																																	?	?	?	?	?	?							?			?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	490	.	.	.	.	.	.	.	.	.	500	.	.	.	.	.	.	.	.	.	510	.	.	.	.	.	.	.	.	.	520	.	.	.	.	.	.	.	.	.	530	.	.	.	.	.	.	.	.	.	540
Sequence		S	R	A	I	N	V	I	P	F	A	L	M	H	N	L	K	H	N	K	V	L	H	E	R	V	I	L	L	T	L	R	T	E	D	A	P	Y	V	H	N	V	R	R	V	Q	I	E	Q	L	S	P	T	F	W	R	V	V	A	S	Y
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	550	.	.	.	.	.	.	.	.	.	560	.	.	.	.	.	.	.	.	.	570	.	.	.	.	.	.	.	.	.	580	.	.	.	.	.	.	.	.	.	590	.	.	.	.	.	.	.	.	.	600
Sequence		G	W	R	E	T	P	N	V	E	E	V	F	H	R	C	G	L	E	G	L	S	C	R	M	M	E	T	S	F	F	M	S	H	E	S	L	I	L	G	K	R	P	W	Y	L	R	L	R	G	K	L	Y	L	L	L	Q	R	N	A	L
Secondary structure
SS confidence
Disorder																																			?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	610	.	.	.	.	.	.	.	.	.	620	.	.
Sequence		R	A	P	D	Q	F	E	I	P	P	N	R	V	I	E	L	G	T	Q	V	E	I
Secondary structure
SS confidence
Disorder																					?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 3gia chain A Region: 3 - 455 Aligned: 419 Modelled: 419 Confidence: 99.8% Identity: 13% PDB header:transport protein Chain: A: PDB Molecule:uncharacterized protein mj0609; PDBTitle: crystal structure of apct transporter Phyre2 PDB 3lrc chain C Region: 3 - 442 Aligned: 393 Modelled: 393 Confidence: 99.5% Identity: 13% PDB header:transport protein Chain: C: PDB Molecule:arginine/agmatine antiporter; PDBTitle: structure of e. coli adic (p1) Phyre2 PDB 2jln chain A Region: 3 - 457 Aligned: 421 Modelled: 427 Confidence: 99.1% Identity: 11% PDB header:membrane protein Chain: A: PDB Molecule:mhp1; PDBTitle: structure of mhp1, a nucleobase-cation-symport-1 family2 transporter Phyre2 PDB 2h3o chain A Region: 101 - 128 Aligned: 28 Modelled: 28 Confidence: 36.4% Identity: 25% PDB header:membrane protein Chain: A: PDB Molecule:merf; PDBTitle: structure of merft, a membrane protein with two trans-2 membrane helices Phyre2 PDB 1waz chain A Region: 101 - 128 Aligned: 28 Modelled: 28 Confidence: 31.5% Identity: 25% PDB header:transport protein Chain: A: PDB Molecule:merf; PDBTitle: nmr structure determination of the bacterial mercury2 transporter, merf, in micelles Phyre2 PDB 2h8p chain C domain 1 Region: 243 - 294 Aligned: 50 Modelled: 52 Confidence: 22.8% Identity: 16% Fold: Voltage-gated potassium channels Superfamily: Voltage-gated potassium channels Family: Voltage-gated potassium channels Phyre2 PDB 2l2t chain A Region: 97 - 125 Aligned: 29 Modelled: 29 Confidence: 17.9% Identity: 34% PDB header:membrane protein Chain: A: PDB Molecule:receptor tyrosine-protein kinase erbb-4; PDBTitle: solution nmr structure of the erbb4 dimeric membrane domain Phyre2 PDB 2zt9 chain H Region: 423 - 442 Aligned: 20 Modelled: 20 Confidence: 14.1% Identity: 30% PDB header:photosynthesis Chain: H: PDB Molecule:cytochrome b6-f complex subunit 8; PDBTitle: crystal structure of the cytochrome b6f complex from nostoc sp. pcc2 7120 Phyre2 PDB 1gsm chain A domain 1 Region: 16 - 40 Aligned: 25 Modelled: 25 Confidence: 11.8% Identity: 32% Fold: Immunoglobulin-like beta-sandwich Superfamily: Immunoglobulin Family: I set domains Phyre2 PDB 1pe4 chain A Region: 19 - 28 Aligned: 10 Modelled: 10 Confidence: 11.8% Identity: 60% Fold: Knottins (small inhibitors, toxins, lectins) Superfamily: Scorpion toxin-like Family: Long-chain scorpion toxins Phyre2 PDB 2e74 chain H domain 1 Region: 423 - 442 Aligned: 20 Modelled: 20 Confidence: 11.5% Identity: 30% Fold: Single transmembrane helix Superfamily: PetN subunit of the cytochrome b6f complex Family: PetN subunit of the cytochrome b6f complex Phyre2 PDB 1vf5 chain U Region: 423 - 442 Aligned: 20 Modelled: 20 Confidence: 11.5% Identity: 30% PDB header:photosynthesis Chain: U: PDB Molecule:protein pet n; PDBTitle: crystal structure of cytochrome b6f complex from m.laminosus Phyre2 PDB 1vf5 chain H Region: 423 - 442 Aligned: 20 Modelled: 20 Confidence: 11.5% Identity: 30% PDB header:photosynthesis Chain: H: PDB Molecule:protein pet n; PDBTitle: crystal structure of cytochrome b6f complex from m.laminosus Phyre2 PDB 2d2c chain H Region: 423 - 442 Aligned: 20 Modelled: 20 Confidence: 11.5% Identity: 30% PDB header:photosynthesis Chain: H: PDB Molecule:cytochrome b6-f complex subunit viii; PDBTitle: crystal structure of cytochrome b6f complex with dbmib from2 m. laminosus Phyre2 PDB 2d2c chain U Region: 423 - 442 Aligned: 20 Modelled: 20 Confidence: 11.5% Identity: 30% PDB header:photosynthesis Chain: U: PDB Molecule:cytochrome b6-f complex subunit viii; PDBTitle: crystal structure of cytochrome b6f complex with dbmib from2 m. laminosus Phyre2 PDB 2e75 chain H Region: 423 - 442 Aligned: 20 Modelled: 20 Confidence: 10.6% Identity: 30% PDB header:photosynthesis Chain: H: PDB Molecule:cytochrome b6-f complex subunit 8; PDBTitle: crystal structure of the cytochrome b6f complex with 2-nonyl-4-2 hydroxyquinoline n-oxide (nqno) from m.laminosus Phyre2 PDB 2e76 chain H Region: 423 - 442 Aligned: 20 Modelled: 20 Confidence: 10.6% Identity: 30% PDB header:photosynthesis Chain: H: PDB Molecule:cytochrome b6-f complex subunit 8; PDBTitle: crystal structure of the cytochrome b6f complex with tridecyl-2 stigmatellin (tds) from m.laminosus Phyre2 PDB 2e74 chain H Region: 423 - 442 Aligned: 20 Modelled: 20 Confidence: 10.6% Identity: 30% PDB header:photosynthesis Chain: H: PDB Molecule:cytochrome b6-f complex subunit 8; PDBTitle: crystal structure of the cytochrome b6f complex from m.laminosus Phyre2 PDB 2kn8 chain A Region: 269 - 308 Aligned: 40 Modelled: 40 Confidence: 9.1% Identity: 30% PDB header:protein binding, dna binding protein Chain: A: PDB Molecule:dna cleavage and packaging protein large subunit, ul89; PDBTitle: nmr structure of the c-terminal domain of pul89 Phyre2 PDB 3ief chain A Region: 114 - 129 Aligned: 16 Modelled: 16 Confidence: 7.3% Identity: 31% PDB header:transferase, rna binding protein Chain: A: PDB Molecule:trna (guanine-n(1)-)-methyltransferase; PDBTitle: crystal structure of trna guanine-n1-methyltransferase from2 bartonella henselae using mpcs. Phyre2 1 c3giaA_ 2 c3lrcC_ 3 c2jlnA_ 4 c2h3oA_ 5 c1wazA_ 6 d2h8pc1 7 c2l2tA_ 8 c2zt9H_ 9 d1gsma1 10 d1pe4a_ 11 d2e74h1 12 c1vf5U_ 13 c1vf5H_ 14 c2d2cH_ 15 c2d2cU_ 16 c2e75H_ 17 c2e76H_ 18 c2e74H_ 19 c2kn8A_ 20 c3iefA_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 c3giaA_ 99.8 13 PDB header:transport protein Chain: A: PDB Molecule:uncharacterized protein mj0609; PDBTitle: crystal structure of apct transporter 2 c3lrcC_ 99.5 13 PDB header:transport protein Chain: C: PDB Molecule:arginine/agmatine antiporter; PDBTitle: structure of e. coli adic (p1) 3 c2jlnA_ 99.1 11 PDB header:membrane protein Chain: A: PDB Molecule:mhp1; PDBTitle: structure of mhp1, a nucleobase-cation-symport-1 family2 transporter 4 c2h3oA_ 36.4 25 PDB header:membrane protein Chain: A: PDB Molecule:merf; PDBTitle: structure of merft, a membrane protein with two trans-2 membrane helices 5 c1wazA_ 31.5 25 PDB header:transport protein Chain: A: PDB Molecule:merf; PDBTitle: nmr structure determination of the bacterial mercury2 transporter, merf, in micelles 6 d2h8pc1 22.8 16 Fold:Voltage-gated potassium channels Superfamily:Voltage-gated potassium channels Family:Voltage-gated potassium channels 7 c2l2tA_ 17.9 34 PDB header:membrane protein Chain: A: PDB Molecule:receptor tyrosine-protein kinase erbb-4; PDBTitle: solution nmr structure of the erbb4 dimeric membrane domain 8 c2zt9H_ 14.1 30 PDB header:photosynthesis Chain: H: PDB Molecule:cytochrome b6-f complex subunit 8; PDBTitle: crystal structure of the cytochrome b6f complex from nostoc sp. pcc2 7120 9 d1gsma1 11.8 32 Fold:Immunoglobulin-like beta-sandwich Superfamily:Immunoglobulin Family:I set domains 10 d1pe4a_ 11.8 60 Fold:Knottins (small inhibitors, toxins, lectins) Superfamily:Scorpion toxin-like Family:Long-chain scorpion toxins 11 d2e74h1 11.5 30 Fold:Single transmembrane helix Superfamily:PetN subunit of the cytochrome b6f complex Family:PetN subunit of the cytochrome b6f complex 12 c1vf5U_ 11.5 30 PDB header:photosynthesis Chain: U: PDB Molecule:protein pet n; PDBTitle: crystal structure of cytochrome b6f complex from m.laminosus 13 c1vf5H_ 11.5 30 PDB header:photosynthesis Chain: H: PDB Molecule:protein pet n; PDBTitle: crystal structure of cytochrome b6f complex from m.laminosus 14 c2d2cH_ 11.5 30 PDB header:photosynthesis Chain: H: PDB Molecule:cytochrome b6-f complex subunit viii; PDBTitle: crystal structure of cytochrome b6f complex with dbmib from2 m. laminosus 15 c2d2cU_ 11.5 30 PDB header:photosynthesis Chain: U: PDB Molecule:cytochrome b6-f complex subunit viii; PDBTitle: crystal structure of cytochrome b6f complex with dbmib from2 m. laminosus 16 c2e75H_ 10.6 30 PDB header:photosynthesis Chain: H: PDB Molecule:cytochrome b6-f complex subunit 8; PDBTitle: crystal structure of the cytochrome b6f complex with 2-nonyl-4-2 hydroxyquinoline n-oxide (nqno) from m.laminosus 17 c2e76H_ 10.6 30 PDB header:photosynthesis Chain: H: PDB Molecule:cytochrome b6-f complex subunit 8; PDBTitle: crystal structure of the cytochrome b6f complex with tridecyl-2 stigmatellin (tds) from m.laminosus 18 c2e74H_ 10.6 30 PDB header:photosynthesis Chain: H: PDB Molecule:cytochrome b6-f complex subunit 8; PDBTitle: crystal structure of the cytochrome b6f complex from m.laminosus 19 c2kn8A_ 9.1 30 PDB header:protein binding, dna binding protein Chain: A: PDB Molecule:dna cleavage and packaging protein large subunit, ul89; PDBTitle: nmr structure of the c-terminal domain of pul89 20 c3iefA_ 7.3 31 PDB header:transferase, rna binding protein Chain: A: PDB Molecule:trna (guanine-n(1)-)-methyltransferase; PDBTitle: crystal structure of trna guanine-n1-methyltransferase from2 bartonella henselae using mpcs. 21 d1p9pa_ not modelled 7.0 25 Fold:alpha/beta knot Superfamily:alpha/beta knot Family:tRNA(m1G37)-methyltransferase TrmD 22 c2l35A_ not modelled 6.9 24 PDB header:protein binding Chain: A: PDB Molecule:dap12-nkg2c_tm; PDBTitle: structure of the dap12-nkg2c transmembrane heterotrimer 23 d1qaza_ not modelled 6.7 6 Fold:alpha/alpha toroid Superfamily:Chondroitin AC/alginate lyase Family:Alginate lyase A1-III 24 c1oy5B_ not modelled 6.7 25 PDB header:transferase Chain: B: PDB Molecule:trna (guanine-n(1)-)-methyltransferase; PDBTitle: crystal structure of trna (m1g37) methyltransferase from aquifex2 aeolicus 25 d1oy5a_ not modelled 6.7 25 Fold:alpha/beta knot Superfamily:alpha/beta knot Family:tRNA(m1G37)-methyltransferase TrmD 26 d1bf2a1 not modelled 6.4 28 Fold:Immunoglobulin-like beta-sandwich Superfamily:E set domains Family:E-set domains of sugar-utilizing enzymes 27 c3quvB_ not modelled 6.4 25 PDB header:transferase Chain: B: PDB Molecule:trna (guanine-n(1)-)-methyltransferase; PDBTitle: crystal structure of a trna-guanine-n1-methyltransferase from2 mycobacterium abscessus 28 d1uala_ not modelled 6.4 38 Fold:alpha/beta knot Superfamily:alpha/beta knot Family:tRNA(m1G37)-methyltransferase TrmD 29 c3ky7A_ not modelled 6.3 44 PDB header:transferase Chain: A: PDB Molecule:trna (guanine-n(1)-)-methyltransferase; PDBTitle: 2.35 angstrom resolution crystal structure of a putative trna2 (guanine-7-)-methyltransferase (trmd) from staphylococcus aureus3 subsp. aureus mrsa252 30 d2axtz1 not modelled 6.2 28 Fold:Transmembrane helix hairpin Superfamily:PsbZ-like Family:PsbZ-like 31 d1oz2a3 not modelled 6.1 12 Fold:SH3-like barrel Superfamily:Tudor/PWWP/MBT Family:MBT repeat 32 c2xq2A_ not modelled 5.8 14 PDB header:transport protein Chain: A: PDB Molecule:sodium/glucose cotransporter; PDBTitle: structure of the k294a mutant of vsglt 33 c3knuD_ not modelled 5.5 19 PDB header:transferase Chain: D: PDB Molecule:trna (guanine-n(1)-)-methyltransferase; PDBTitle: crystal structure of trna (guanine-n1)-methyltransferase from2 anaplasma phagocytophilum 34 c1nyjC_ not modelled 5.5 40 PDB header:viral protein Chain: C: PDB Molecule:matrix protein m2; PDBTitle: the closed state structure of m2 protein h+ channel by2 solid state nmr spectroscopy 35 c1nyjB_ not modelled 5.5 40 PDB header:viral protein Chain: B: PDB Molecule:matrix protein m2; PDBTitle: the closed state structure of m2 protein h+ channel by2 solid state nmr spectroscopy 36 c2kqtC_ not modelled 5.5 40 PDB header:transport protein Chain: C: PDB Molecule:m2 protein; PDBTitle: solid-state nmr structure of the m2 transmembrane peptide of the2 influenza a virus in dmpc lipid bilayers bound to deuterated3 amantadine 37 c2kqtA_ not modelled 5.5 40 PDB header:transport protein Chain: A: PDB Molecule:m2 protein; PDBTitle: solid-state nmr structure of the m2 transmembrane peptide of the2 influenza a virus in dmpc lipid bilayers bound to deuterated3 amantadine 38 c2kqtB_ not modelled 5.5 40 PDB header:transport protein Chain: B: PDB Molecule:m2 protein; PDBTitle: solid-state nmr structure of the m2 transmembrane peptide of the2 influenza a virus in dmpc lipid bilayers bound to deuterated3 amantadine 39 c1nyjD_ not modelled 5.5 40 PDB header:viral protein Chain: D: PDB Molecule:matrix protein m2; PDBTitle: the closed state structure of m2 protein h+ channel by2 solid state nmr spectroscopy 40 c2kqtD_ not modelled 5.5 40 PDB header:transport protein Chain: D: PDB Molecule:m2 protein; PDBTitle: solid-state nmr structure of the m2 transmembrane peptide of the2 influenza a virus in dmpc lipid bilayers bound to deuterated3 amantadine 41 c1nyjA_ not modelled 5.5 40 PDB header:viral protein Chain: A: PDB Molecule:matrix protein m2; PDBTitle: the closed state structure of m2 protein h+ channel by2 solid state nmr spectroscopy 42 c1mp6A_ not modelled 5.5 40 PDB header:membrane protein Chain: A: PDB Molecule:matrix protein m2; PDBTitle: structure of the transmembrane region of the m2 protein h+2 channel by solid state nmr spectroscopy 43 c3c66B_ not modelled 5.4 18 PDB header:transferase Chain: B: PDB Molecule:poly(a) polymerase; PDBTitle: yeast poly(a) polymerase in complex with fip1 residues 80-105 44 d1moua_ not modelled 5.1 13 Fold:GFP-like Superfamily:GFP-like Family:Fluorescent proteins

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

---

Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0