Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	R	A	K	L	L	G	I	V	L	T	T	P	I	A	I	S	S	F	A	S	T	E	T	L	S	F	T	P	D	N	I	N	A	D	I	S	L	G	T	L	S	G	K	T	K	E	R	V	Y	L	A	E	E	G	G	R	K	V	S
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?									?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?							?													?		?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		Q	L	D	W	K	F	N	N	A	A	I	I	K	G	A	I	N	W	D	L	M	P	Q	I	S	I	G	A	A	G	W	T	T	L	G	S	R	G	G	N	M	V	D	Q	D	W	M	D	S	S	N	P	G	T	W	T	D	E	S	R
Secondary structure
SS confidence
Disorder															?									?	?					?					?	?	?	?	?											?	?	?	?	?	?	?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		H	P	D	T	Q	L	N	Y	A	N	E	F	D	L	N	I	K	G	W	L	L	N	E	P	N	Y	R	L	G	L	M	A	G	Y	Q	E	S	R	Y	S	F	T	A	R	G	G	S	Y	I	Y	S	S	E	E	G	F	R	D	D	I
Secondary structure
SS confidence
Disorder			?																																																			?	?	?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		G	S	F	P	N	G	E	R	A	I	G	Y	K	Q	R	F	K	M	P	Y	I	G	L	T	G	S	Y	R	Y	E	D	F	E	L	G	G	T	F	K	Y	S	G	W	V	E	S	S	D	N	D	E	H	Y	D	P	G	K	R	I	T
Secondary structure
SS confidence
Disorder						?																																																					?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		Y	R	S	K	V	K	D	Q	N	Y	Y	S	V	A	V	N	A	G	Y	Y	V	T	P	N	A	K	V	Y	V	E	G	A	W	N	R	V	T	N	K	K	G	N	T	S	L	Y	D	H	N	N	N	T	S	D	Y	S	K	N	G	A
Secondary structure
SS confidence
Disorder			?			?		?	?																																											?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.
Sequence		G	I	E	N	Y	N	F	I	T	T	A	G	L	K	Y	T	F
Secondary structure
SS confidence
Disorder													?					?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 1i78 chain A Region: 21 - 317 Aligned: 297 Modelled: 297 Confidence: 100.0% Identity: 99% Fold: Transmembrane beta-barrels Superfamily: OMPT-like Family: Outer membrane protease OMPT Phyre2 PDB 2x4m chain D Region: 25 - 317 Aligned: 285 Modelled: 293 Confidence: 100.0% Identity: 52% PDB header:hydrolase Chain: D: PDB Molecule:coagulase/fibrinolysin; PDBTitle: yersinia pestis plasminogen activator pla Phyre2 PDB 1p4t chain A Region: 143 - 317 Aligned: 132 Modelled: 132 Confidence: 98.2% Identity: 20% Fold: Transmembrane beta-barrels Superfamily: OMPA-like Family: Outer membrane protein Phyre2 PDB 2qom chain B Region: 128 - 317 Aligned: 151 Modelled: 151 Confidence: 98.1% Identity: 13% PDB header:hydrolase Chain: B: PDB Molecule:serine protease espp; PDBTitle: the crystal structure of the e.coli espp autotransporter beta-domain. Phyre2 PDB 3slj chain A Region: 127 - 317 Aligned: 153 Modelled: 153 Confidence: 98.1% Identity: 10% PDB header:protein transport Chain: A: PDB Molecule:serine protease espp; PDBTitle: pre-cleavage structure of the autotransporter espp - n1023a mutant Phyre2 PDB 2f1t chain B Region: 142 - 317 Aligned: 156 Modelled: 176 Confidence: 98.0% Identity: 11% PDB header:membrane protein Chain: B: PDB Molecule:outer membrane protein w; PDBTitle: outer membrane protein ompw Phyre2 PDB 2x27 chain X Region: 135 - 317 Aligned: 171 Modelled: 178 Confidence: 98.0% Identity: 11% PDB header:membrane protein Chain: X: PDB Molecule:outer membrane protein oprg; PDBTitle: crystal structure of the outer membrane protein oprg from2 pseudomonas aeruginosa Phyre2 PDB 3dwo chain X Region: 9 - 317 Aligned: 281 Modelled: 299 Confidence: 97.9% Identity: 14% PDB header:membrane protein Chain: X: PDB Molecule:probable outer membrane protein; PDBTitle: crystal structure of a pseudomonas aeruginosa fadl homologue Phyre2 PDB 2k0l chain A Region: 142 - 317 Aligned: 144 Modelled: 159 Confidence: 97.7% Identity: 16% PDB header:membrane protein Chain: A: PDB Molecule:outer membrane protein a; PDBTitle: nmr structure of the transmembrane domain of the outer2 membrane protein a from klebsiella pneumoniae in dhpc3 micelles. Phyre2 PDB 3aeh chain B Region: 128 - 317 Aligned: 123 Modelled: 123 Confidence: 97.2% Identity: 15% PDB header:hydrolase Chain: B: PDB Molecule:hemoglobin-binding protease hbp autotransporter; PDBTitle: integral membrane domain of autotransporter hbp Phyre2 PDB 1qj8 chain A Region: 133 - 317 Aligned: 140 Modelled: 140 Confidence: 97.1% Identity: 16% Fold: Transmembrane beta-barrels Superfamily: OMPA-like Family: Outer membrane protein Phyre2 PDB 1g90 chain A Region: 143 - 317 Aligned: 138 Modelled: 158 Confidence: 97.1% Identity: 18% Fold: Transmembrane beta-barrels Superfamily: OMPA-like Family: Outer membrane protein Phyre2 PDB 3qra chain A Region: 149 - 317 Aligned: 134 Modelled: 144 Confidence: 97.1% Identity: 15% PDB header:cell invasion Chain: A: PDB Molecule:attachment invasion locus protein; PDBTitle: the crystal structure of ail, the attachment invasion locus protein of2 yersinia pestis Phyre2 PDB 1t16 chain A Region: 34 - 317 Aligned: 277 Modelled: 284 Confidence: 97.0% Identity: 12% Fold: Transmembrane beta-barrels Superfamily: Porins Family: Outer membrane protein transport protein Phyre2 PDB 2lhf chain A Region: 243 - 317 Aligned: 67 Modelled: 75 Confidence: 96.7% Identity: 10% PDB header:membrane protein Chain: A: PDB Molecule:outer membrane protein h1; PDBTitle: solution structure of outer membrane protein h (oprh) from p.2 aeruginosa in dhpc micelles Phyre2 PDB 1qjp chain A Region: 139 - 317 Aligned: 121 Modelled: 131 Confidence: 96.7% Identity: 23% Fold: Transmembrane beta-barrels Superfamily: OMPA-like Family: Outer membrane protein Phyre2 PDB 3nb3 chain C Region: 139 - 317 Aligned: 121 Modelled: 131 Confidence: 96.1% Identity: 21% PDB header:virus Chain: C: PDB Molecule:outer membrane protein a; PDBTitle: the host outer membrane proteins ompa and ompc are packed at specific2 sites in the shigella phage sf6 virion as structural components Phyre2 PDB 2fgq chain X domain 1 Region: 148 - 317 Aligned: 140 Modelled: 150 Confidence: 96.1% Identity: 14% Fold: Transmembrane beta-barrels Superfamily: Porins Family: Porin Phyre2 PDB 3brz chain A Region: 69 - 317 Aligned: 235 Modelled: 244 Confidence: 96.1% Identity: 10% PDB header:transport protein Chain: A: PDB Molecule:todx; PDBTitle: crystal structure of the pseudomonas putida toluene2 transporter todx Phyre2 PDB 2iwv chain D Region: 72 - 317 Aligned: 167 Modelled: 191 Confidence: 95.9% Identity: 16% PDB header:ion channel Chain: D: PDB Molecule:outer membrane protein g; PDBTitle: structure of the monomeric outer membrane porin ompg in the2 open and closed conformation Phyre2 1 d1i78a_ 2 c2x4mD_ 3 d1p4ta_ 4 c2qomB_ 5 c3sljA_ 6 c2f1tB_ 7 c2x27X_ 8 c3dwoX_ 9 c2k0lA_ 10 c3aehB_ 11 d1qj8a_ 12 d1g90a_ 13 c3qraA_ 14 d1t16a_ 15 c2lhfA_ 16 d1qjpa_ 17 c3nb3C_ 18 d2fgqx1 19 c3brzA_ 20 c2iwvD_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 d1i78a_ 100.0 99 Fold:Transmembrane beta-barrels Superfamily:OMPT-like Family:Outer membrane protease OMPT 2 c2x4mD_ 100.0 52 PDB header:hydrolase Chain: D: PDB Molecule:coagulase/fibrinolysin; PDBTitle: yersinia pestis plasminogen activator pla 3 d1p4ta_ 98.2 20 Fold:Transmembrane beta-barrels Superfamily:OMPA-like Family:Outer membrane protein 4 c2qomB_ 98.1 13 PDB header:hydrolase Chain: B: PDB Molecule:serine protease espp; PDBTitle: the crystal structure of the e.coli espp autotransporter beta-domain. 5 c3sljA_ 98.1 10 PDB header:protein transport Chain: A: PDB Molecule:serine protease espp; PDBTitle: pre-cleavage structure of the autotransporter espp - n1023a mutant 6 c2f1tB_ 98.0 11 PDB header:membrane protein Chain: B: PDB Molecule:outer membrane protein w; PDBTitle: outer membrane protein ompw 7 c2x27X_ 98.0 11 PDB header:membrane protein Chain: X: PDB Molecule:outer membrane protein oprg; PDBTitle: crystal structure of the outer membrane protein oprg from2 pseudomonas aeruginosa 8 c3dwoX_ 97.9 14 PDB header:membrane protein Chain: X: PDB Molecule:probable outer membrane protein; PDBTitle: crystal structure of a pseudomonas aeruginosa fadl homologue 9 c2k0lA_ 97.7 16 PDB header:membrane protein Chain: A: PDB Molecule:outer membrane protein a; PDBTitle: nmr structure of the transmembrane domain of the outer2 membrane protein a from klebsiella pneumoniae in dhpc3 micelles. 10 c3aehB_ 97.2 15 PDB header:hydrolase Chain: B: PDB Molecule:hemoglobin-binding protease hbp autotransporter; PDBTitle: integral membrane domain of autotransporter hbp 11 d1qj8a_ 97.1 16 Fold:Transmembrane beta-barrels Superfamily:OMPA-like Family:Outer membrane protein 12 d1g90a_ 97.1 18 Fold:Transmembrane beta-barrels Superfamily:OMPA-like Family:Outer membrane protein 13 c3qraA_ 97.1 15 PDB header:cell invasion Chain: A: PDB Molecule:attachment invasion locus protein; PDBTitle: the crystal structure of ail, the attachment invasion locus protein of2 yersinia pestis 14 d1t16a_ 97.0 12 Fold:Transmembrane beta-barrels Superfamily:Porins Family:Outer membrane protein transport protein 15 c2lhfA_ 96.7 10 PDB header:membrane protein Chain: A: PDB Molecule:outer membrane protein h1; PDBTitle: solution structure of outer membrane protein h (oprh) from p.2 aeruginosa in dhpc micelles 16 d1qjpa_ 96.7 23 Fold:Transmembrane beta-barrels Superfamily:OMPA-like Family:Outer membrane protein 17 c3nb3C_ 96.1 21 PDB header:virus Chain: C: PDB Molecule:outer membrane protein a; PDBTitle: the host outer membrane proteins ompa and ompc are packed at specific2 sites in the shigella phage sf6 virion as structural components 18 d2fgqx1 96.1 14 Fold:Transmembrane beta-barrels Superfamily:Porins Family:Porin 19 c3brzA_ 96.1 10 PDB header:transport protein Chain: A: PDB Molecule:todx; PDBTitle: crystal structure of the pseudomonas putida toluene2 transporter todx 20 c2iwvD_ 95.9 16 PDB header:ion channel Chain: D: PDB Molecule:outer membrane protein g; PDBTitle: structure of the monomeric outer membrane porin ompg in the2 open and closed conformation 21 d1uynx_ not modelled 95.2 13 Fold:Transmembrane beta-barrels Superfamily:Autotransporter Family:Autotransporter 22 c3a2rX_ not modelled 94.7 19 PDB header:membrane protein Chain: X: PDB Molecule:outer membrane protein ii; PDBTitle: crystal structure of outer membrane protein porb from neisseria2 meningitidis 23 c3kvnA_ not modelled 94.5 13 PDB header:hydrolase Chain: A: PDB Molecule:esterase esta; PDBTitle: crystal structure of the full-length autotransporter esta from2 pseudomonas aeruginosa 24 c3qq2C_ not modelled 94.0 14 PDB header:membrane protein/protein transport Chain: C: PDB Molecule:brka autotransporter; PDBTitle: crystal structure of the beta domain of the bordetella autotransporter2 brka 25 c2jmmA_ not modelled 93.6 21 PDB header:membrane protein Chain: A: PDB Molecule:outer membrane protein a; PDBTitle: nmr solution structure of a minimal transmembrane beta-2 barrel platform protein 26 c3dzmB_ not modelled 93.3 18 PDB header:unknown function Chain: B: PDB Molecule:hypothetical conserved protein; PDBTitle: crystal structure of a major outer membrane protein from thermus2 thermophilus hb27 27 d1phoa_ not modelled 90.6 16 Fold:Transmembrane beta-barrels Superfamily:Porins Family:Porin 28 d2zfga1 not modelled 87.5 16 Fold:Transmembrane beta-barrels Superfamily:Porins Family:Porin 29 d1osma_ not modelled 85.6 19 Fold:Transmembrane beta-barrels Superfamily:Porins Family:Porin 30 c3bryB_ not modelled 81.7 11 PDB header:transport protein Chain: B: PDB Molecule:tbux; PDBTitle: crystal structure of the ralstonia pickettii toluene2 transporter tbux 31 c3nsgA_ not modelled 79.1 16 PDB header:membrane protein Chain: A: PDB Molecule:outer membrane protein f; PDBTitle: crystal structure of ompf, an outer membrane protein from salmonella2 typhi 32 d2gufa1 not modelled 73.8 26 Fold:Transmembrane beta-barrels Superfamily:Porins Family:Ligand-gated protein channel 33 d1af6a_ not modelled 68.3 17 Fold:Transmembrane beta-barrels Superfamily:Porins Family:Maltoporin-like 34 c2iahA_ not modelled 53.6 16 PDB header:membrane protein Chain: A: PDB Molecule:ferripyoverdine receptor; PDBTitle: crystal structure of the ferripyoverdine receptor of the outer2 membrane of pseudomonas aeruginosa bound to ferripyoverdine. 35 c2k4tA_ not modelled 51.0 11 PDB header:membrane protein,apoptosis Chain: A: PDB Molecule:voltage-dependent anion-selective channel PDBTitle: solution structure of human vdac-1 in ldao micelles 36 d2mpra_ not modelled 47.9 22 Fold:Transmembrane beta-barrels Superfamily:Porins Family:Maltoporin-like 37 c3cslB_ not modelled 38.9 19 PDB header:membrane protein/heme binding protein Chain: B: PDB Molecule:hasr protein; PDBTitle: structure of the serratia marcescens hemophore receptor hasr in2 complex with its hemophore hasa and heme 38 d3prna_ not modelled 33.2 27 Fold:Transmembrane beta-barrels Superfamily:Porins Family:Porin 39 d2pora_ not modelled 18.4 19 Fold:Transmembrane beta-barrels Superfamily:Porins Family:Porin 40 c3gf6B_ not modelled 17.5 18 PDB header:unknown function Chain: B: PDB Molecule:uncharacterized bacterial lipoprotein; PDBTitle: crystal structure of a bacterial lipoprotein (bt_1233) from2 bacteroides thetaiotaomicron vpi-5482 at 1.69 a resolution 41 d1a0tp_ not modelled 12.3 11 Fold:Transmembrane beta-barrels Superfamily:Porins Family:Maltoporin-like 42 c3ghnA_ not modelled 11.6 7 PDB header:hydrolase Chain: A: PDB Molecule:a disintegrin and metalloproteinase with thrombospondin PDBTitle: crystal structure of the exosite-containing fragment of human adamts132 (form-2) 43 d1o75a1 not modelled 7.5 13 Fold:Immunoglobulin-like beta-sandwich Superfamily:Tp47 lipoprotein, middle and C-terminal domains Family:Tp47 lipoprotein, middle and C-terminal domains

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0