Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	M	N	I	E	E	L	R	K	I	F	C	E	D	G	L	Y	A	V	C	V	E	N	G	N	L	V	S	H	Y	R	I	M	C	L	R	K	N	G	A	A	L	I	N	F	V	D	A	R	V	T	D	G	F	I	L	R	E	G	E
Secondary structure
SS confidence
Disorder		?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.
Sequence		F	V	T	S	L	Q	A	L	K	E	I	G	I	K	A	G	F	S	A	F	S	G	E
Secondary structure
SS confidence
Disorder												?						?	?	?	?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 3hiz chain B Region: 13 - 39 Aligned: 25 Modelled: 27 Confidence: 35.8% Identity: 20% PDB header:transferase/oncoprotein Chain: B: PDB Molecule:phosphatidylinositol 3-kinase regulatory subunit PDBTitle: crystal structure of p110alpha h1047r mutant in complex with2 nish2 of p85alpha Phyre2 PDB 2shp chain A domain 2 Region: 13 - 70 Aligned: 43 Modelled: 43 Confidence: 31.7% Identity: 26% Fold: SH2-like Superfamily: SH2 domain Family: SH2 domain Phyre2 PDB 2eyv chain A domain 1 Region: 13 - 70 Aligned: 58 Modelled: 58 Confidence: 30.2% Identity: 26% Fold: SH2-like Superfamily: SH2 domain Family: SH2 domain Phyre2 PDB 1nzj chain A Region: 40 - 74 Aligned: 35 Modelled: 35 Confidence: 21.6% Identity: 37% Fold: Adenine nucleotide alpha hydrolase-like Superfamily: Nucleotidylyl transferase Family: Class I aminoacyl-tRNA synthetases (RS), catalytic domain Phyre2 PDB 2izv chain A domain 2 Region: 13 - 34 Aligned: 22 Modelled: 22 Confidence: 20.7% Identity: 32% Fold: SH2-like Superfamily: SH2 domain Family: SH2 domain Phyre2 PDB 1rpy chain A Region: 13 - 32 Aligned: 20 Modelled: 20 Confidence: 20.5% Identity: 15% Fold: SH2-like Superfamily: SH2 domain Family: SH2 domain Phyre2 PDB 3rrw chain B Region: 60 - 81 Aligned: 22 Modelled: 22 Confidence: 19.6% Identity: 23% PDB header:plant protein Chain: B: PDB Molecule:thylakoid lumenal 29 kda protein, chloroplastic; PDBTitle: crystal structure of the tl29 protein from arabidopsis thaliana Phyre2 PDB 1lc0 chain A domain 2 Region: 74 - 82 Aligned: 9 Modelled: 9 Confidence: 17.3% Identity: 67% Fold: FwdE/GAPDH domain-like Superfamily: Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain Family: Biliverdin reductase Phyre2 PDB 1a81 chain E domain 1 Region: 13 - 70 Aligned: 44 Modelled: 44 Confidence: 15.5% Identity: 25% Fold: SH2-like Superfamily: SH2 domain Family: SH2 domain Phyre2 PDB 2lct chain A Region: 13 - 70 Aligned: 43 Modelled: 45 Confidence: 14.9% Identity: 21% PDB header:signaling protein Chain: A: PDB Molecule:proto-oncogene vav; PDBTitle: solution structure of the vav1 sh2 domain complexed with a syk-derived2 doubly phosphorylated peptide Phyre2 PDB 2k7c chain A Region: 1 - 11 Aligned: 11 Modelled: 11 Confidence: 14.6% Identity: 45% PDB header:metal binding protein Chain: A: PDB Molecule:calcium-binding protein 1; PDBTitle: nmr structure of mg2+-bound cabp1 c-domain Phyre2 PDB 2oq1 chain A domain 1 Region: 15 - 70 Aligned: 42 Modelled: 42 Confidence: 12.7% Identity: 19% Fold: SH2-like Superfamily: SH2 domain Family: SH2 domain Phyre2 PDB 1j09 chain A domain 2 Region: 40 - 74 Aligned: 35 Modelled: 35 Confidence: 12.4% Identity: 49% Fold: Adenine nucleotide alpha hydrolase-like Superfamily: Nucleotidylyl transferase Family: Class I aminoacyl-tRNA synthetases (RS), catalytic domain Phyre2 PDB 1k9a chain B Region: 13 - 70 Aligned: 43 Modelled: 43 Confidence: 12.1% Identity: 33% PDB header:transferase Chain: B: PDB Molecule:carboxyl-terminal src kinase; PDBTitle: crystal structure analysis of full-length carboxyl-terminal2 src kinase at 2.5 a resolution Phyre2 PDB 2vif chain A Region: 13 - 34 Aligned: 22 Modelled: 22 Confidence: 11.1% Identity: 18% PDB header:signaling protein Chain: A: PDB Molecule:suppressor of cytokine signalling 6; PDBTitle: crystal structure of socs6 sh2 domain in complex with a c-kit2 phosphopeptide Phyre2 PDB 1ifq chain A Region: 48 - 71 Aligned: 24 Modelled: 24 Confidence: 10.4% Identity: 38% Fold: Profilin-like Superfamily: SNARE-like Family: Synatpobrevin N-terminal domain Phyre2 PDB 2eo6 chain A Region: 17 - 39 Aligned: 23 Modelled: 23 Confidence: 10.3% Identity: 9% PDB header:signaling protein Chain: A: PDB Molecule:b-cell linker protein; PDBTitle: solution structure of the sh2 domain from mouse b-cell2 linker protein blnk Phyre2 PDB 2cr4 chain A Region: 17 - 33 Aligned: 17 Modelled: 17 Confidence: 9.7% Identity: 41% PDB header:signaling protein Chain: A: PDB Molecule:sh3 domain-binding protein 2; PDBTitle: solution structure of the sh2 domain of human sh3bp2 protein Phyre2 PDB 2x8r chain E Region: 34 - 81 Aligned: 45 Modelled: 48 Confidence: 9.0% Identity: 22% PDB header:hydrolase Chain: E: PDB Molecule:glycosyl hydrolase; PDBTitle: the structure of a family gh25 lysozyme from aspergillus2 fumigatus Phyre2 PDB 1wj5 chain A Region: 41 - 83 Aligned: 43 Modelled: 43 Confidence: 8.9% Identity: 23% Fold: DNA/RNA-binding 3-helical bundle Superfamily: "Winged helix" DNA-binding domain Family: An Obfc1 domain Phyre2 1 c3hizB_ 2 d2shpa2 3 d2eyva1 4 d1nzja_ 5 d2izva2 6 d1rpya_ 7 c3rrwB_ 8 d1lc0a2 9 d1a81e1 10 c2lctA_ 11 c2k7cA_ 12 d2oq1a1 13 d1j09a2 14 c1k9aB_ 15 c2vifA_ 16 d1ifqa_ 17 c2eo6A_ 18 c2cr4A_ 19 c2x8rE_ 20 d1wj5a_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 c3hizB_ 35.8 20 PDB header:transferase/oncoprotein Chain: B: PDB Molecule:phosphatidylinositol 3-kinase regulatory subunit PDBTitle: crystal structure of p110alpha h1047r mutant in complex with2 nish2 of p85alpha 2 d2shpa2 31.7 26 Fold:SH2-like Superfamily:SH2 domain Family:SH2 domain 3 d2eyva1 30.2 26 Fold:SH2-like Superfamily:SH2 domain Family:SH2 domain 4 d1nzja_ 21.6 37 Fold:Adenine nucleotide alpha hydrolase-like Superfamily:Nucleotidylyl transferase Family:Class I aminoacyl-tRNA synthetases (RS), catalytic domain 5 d2izva2 20.7 32 Fold:SH2-like Superfamily:SH2 domain Family:SH2 domain 6 d1rpya_ 20.5 15 Fold:SH2-like Superfamily:SH2 domain Family:SH2 domain 7 c3rrwB_ 19.6 23 PDB header:plant protein Chain: B: PDB Molecule:thylakoid lumenal 29 kda protein, chloroplastic; PDBTitle: crystal structure of the tl29 protein from arabidopsis thaliana 8 d1lc0a2 17.3 67 Fold:FwdE/GAPDH domain-like Superfamily:Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain Family:Biliverdin reductase 9 d1a81e1 15.5 25 Fold:SH2-like Superfamily:SH2 domain Family:SH2 domain 10 c2lctA_ 14.9 21 PDB header:signaling protein Chain: A: PDB Molecule:proto-oncogene vav; PDBTitle: solution structure of the vav1 sh2 domain complexed with a syk-derived2 doubly phosphorylated peptide 11 c2k7cA_ 14.6 45 PDB header:metal binding protein Chain: A: PDB Molecule:calcium-binding protein 1; PDBTitle: nmr structure of mg2+-bound cabp1 c-domain 12 d2oq1a1 12.7 19 Fold:SH2-like Superfamily:SH2 domain Family:SH2 domain 13 d1j09a2 12.4 49 Fold:Adenine nucleotide alpha hydrolase-like Superfamily:Nucleotidylyl transferase Family:Class I aminoacyl-tRNA synthetases (RS), catalytic domain 14 c1k9aB_ 12.1 33 PDB header:transferase Chain: B: PDB Molecule:carboxyl-terminal src kinase; PDBTitle: crystal structure analysis of full-length carboxyl-terminal2 src kinase at 2.5 a resolution 15 c2vifA_ 11.1 18 PDB header:signaling protein Chain: A: PDB Molecule:suppressor of cytokine signalling 6; PDBTitle: crystal structure of socs6 sh2 domain in complex with a c-kit2 phosphopeptide 16 d1ifqa_ 10.4 38 Fold:Profilin-like Superfamily:SNARE-like Family:Synatpobrevin N-terminal domain 17 c2eo6A_ 10.3 9 PDB header:signaling protein Chain: A: PDB Molecule:b-cell linker protein; PDBTitle: solution structure of the sh2 domain from mouse b-cell2 linker protein blnk 18 c2cr4A_ 9.7 41 PDB header:signaling protein Chain: A: PDB Molecule:sh3 domain-binding protein 2; PDBTitle: solution structure of the sh2 domain of human sh3bp2 protein 19 c2x8rE_ 9.0 22 PDB header:hydrolase Chain: E: PDB Molecule:glycosyl hydrolase; PDBTitle: the structure of a family gh25 lysozyme from aspergillus2 fumigatus 20 d1wj5a_ 8.9 23 Fold:DNA/RNA-binding 3-helical bundle Superfamily:"Winged helix" DNA-binding domain Family:An Obfc1 domain 21 c3aiiA_ not modelled 8.2 56 PDB header:ligase Chain: A: PDB Molecule:glutamyl-trna synthetase; PDBTitle: archaeal non-discriminating glutamyl-trna synthetase from2 methanothermobacter thermautotrophicus 22 d1a81a1 not modelled 7.6 35 Fold:SH2-like Superfamily:SH2 domain Family:SH2 domain 23 d2c9wa2 not modelled 7.5 23 Fold:SH2-like Superfamily:SH2 domain Family:SH2 domain 24 c2fo0A_ not modelled 7.4 22 PDB header:transferase Chain: A: PDB Molecule:proto-oncogene tyrosine-protein kinase abl1 (1b isoform); PDBTitle: organization of the sh3-sh2 unit in active and inactive forms of the2 c-abl tyrosine kinase 25 c3bv8A_ not modelled 7.4 28 PDB header:transferase Chain: A: PDB Molecule:tetrahydrodipicolinate acetyltransferase; PDBTitle: crystal structure of the n-terminal domain of tetrahydrodipicolinate2 acetyltransferase from staphylococcus aureus 26 c1exdA_ not modelled 7.2 33 PDB header:ligase/rna Chain: A: PDB Molecule:glutaminyl-trna synthetase; PDBTitle: crystal structure of a tight-binding glutamine trna bound2 to glutamine aminoacyl trna synthetase 27 c2c9wA_ not modelled 7.1 23 PDB header:transcription regulation Chain: A: PDB Molecule:suppressor of cytokine signaling 2; PDBTitle: crystal structure of socs-2 in complex with elongin-b and2 elongin-c at 1.9a resolution 28 c2dx0B_ not modelled 6.9 30 PDB header:hydrolase Chain: B: PDB Molecule:phospholipase c, gamma 2; PDBTitle: crystal structure of the n-terminal sh2 domain of mouse2 phospholipase c-gamma 2 29 c3i3tG_ not modelled 6.7 32 PDB header:hydrolase Chain: G: PDB Molecule:ubiquitin carboxyl-terminal hydrolase 21; PDBTitle: crystal structure of covalent ubiquitin-usp21 complex 30 d1bbua1 not modelled 6.6 21 Fold:OB-fold Superfamily:Nucleic acid-binding proteins Family:Anticodon-binding domain 31 c2hz7A_ not modelled 6.4 50 PDB header:ligase Chain: A: PDB Molecule:glutaminyl-trna synthetase; PDBTitle: crystal structure of the glutaminyl-trna synthetase from2 deinococcus radiodurans 32 d1a81e2 not modelled 6.3 35 Fold:SH2-like Superfamily:SH2 domain Family:SH2 domain 33 c3k0zB_ not modelled 6.1 18 PDB header:lyase Chain: B: PDB Molecule:putative polyketide cyclase; PDBTitle: crystal structure of putative polyketide cyclase (np_977253.1) from2 bacillus cereus atcc 10987 at 1.91 a resolution 34 d2adca1 not modelled 6.0 24 Fold:Ferredoxin-like Superfamily:RNA-binding domain, RBD Family:Canonical RBD 35 c2eyzA_ not modelled 6.0 40 PDB header:signaling protein Chain: A: PDB Molecule:v-crk sarcoma virus ct10 oncogene homolog PDBTitle: ct10-regulated kinase isoform ii 36 c2el8A_ not modelled 5.9 22 PDB header:signaling protein Chain: A: PDB Molecule:signal-transducing adaptor protein 2; PDBTitle: solution structure of the human stap2 sh2 domain 37 c2nw0B_ not modelled 5.8 31 PDB header:hydrolase Chain: B: PDB Molecule:plyb; PDBTitle: crystal structure of a lysin 38 c1rqqC_ not modelled 5.7 15 PDB header:transferase/signaling protein Chain: C: PDB Molecule:adaptor protein aps; PDBTitle: crystal structure of the insulin receptor kinase in complex2 with the sh2 domain of aps 39 d1jfxa_ not modelled 5.5 21 Fold:TIM beta/alpha-barrel Superfamily:(Trans)glycosidases Family:1,4-beta-N-acetylmuraminidase 40 d2ah2a2 not modelled 5.4 19 Fold:6-bladed beta-propeller Superfamily:Sialidases Family:Sialidases (neuraminidases) 41 d1nbfa_ not modelled 5.3 31 Fold:Cysteine proteinases Superfamily:Cysteine proteinases Family:Ubiquitin carboxyl-terminal hydrolase, UCH 42 d2g0ta1 not modelled 5.2 52 Fold:P-loop containing nucleoside triphosphate hydrolases Superfamily:P-loop containing nucleoside triphosphate hydrolases Family:Nitrogenase iron protein-like

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0