Phyre Home Retrieve Phyre Job Id
Emaill.a.kelley@imperial.ac.uk
DescriptionP77243
DateThu Jan 5 12:26:44 GMT 2012
Unique Job IDc8b48861b9a1b681

Summary 

Top model
Image coloured by rainbow N → C terminus
Model (left) based on template d1szqa_
Top template information
Fold:2-methylcitrate dehydratase PrpD
Superfamily:2-methylcitrate dehydratase PrpD
Family:2-methylcitrate dehydratase PrpD
Confidence and coverage
Confidence:100.0% Coverage: 98%
473 residues ( 98% of your sequence) have been modelled with 100.0% confidence by the single highest scoring template.
3D viewing
Interactive 3D view in Jmol

Sequence analysis 

Secondary structure and disorder prediction 

   1........10.........20.........30.........40.........50.........60
Sequence  MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDTLGCGLEALEYPACKKL
Secondary structure 



















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Disorder  ????????????















































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   .........70.........80.........90.........100.........110.........120
Sequence  LGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFNDTWLAAEWGHPSDNLGGI
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Disorder 




???

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?






































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   .........130.........140.........150.........160.........170.........180
Sequence  LATADWLSRNAVASGKAPLTMKQVLTAMIKAHEIQGCIALENSFNRVGLDHVLLVKVAST
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Disorder 






???????????









































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   .........190.........200.........210.........220.........230.........240
Sequence  AVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTGTRKSWAAGDATSRAVRLALMAK
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???????






















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   .........250.........260.........270.........280.........290.........300
Sequence  TGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSYVMENVLFKISFPAEFHSQTAVEAA
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Disorder 




??




















































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   .........310.........320.........330.........340.........350.........360
Sequence  MTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDKKGPLNNPADRDHCIQYMVAIPLLFGR
Secondary structure 
















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Disorder 








??
?

























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   .........370.........380.........390.........400.........410.........420
Sequence  LTAADYEDNVAQDKRIDALREKINCFEDPAFTADYHDPEKRAIANAITLEFTDGTRFEEV
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??
?
?

















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   .........430.........440.........450.........460.........470.........480
Sequence  VVEYPIGHARRRQDGIPKLVDKFKINLARQFPTRQQQRILEVSLDRARLEQMPVNEYLDL
Secondary structure 
























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?
??
?











































?
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   ...
Sequence  YVI
Secondary structure 

SS confidence 


Disorder  ???
Disorder confidence 


 

Confidence Key
High(9)                    Low (0)
?Disordered
Alpha helix
Beta strand

Domain analysis 

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

RankAligned region

PDB 1szq chain A

3D model

Region: 11 - 483
Aligned: 473
Modelled: 473
Confidence: 100.0%
Identity: 97%
Fold: 2-methylcitrate dehydratase PrpD
Superfamily: 2-methylcitrate dehydratase PrpD
Family: 2-methylcitrate dehydratase PrpD

Phyre2

PDB 2hp0 chain A

3D model

Region: 14 - 477
Aligned: 434
Modelled: 459
Confidence: 100.0%
Identity: 16%
PDB header:isomerase
Chain: A: PDB Molecule:ids-epimerase;
PDBTitle: crystal structure of iminodisuccinate epimerase

Phyre2

PDB 2a1j chain A domain 1

3D model

Region: 449 - 481
Aligned: 33
Modelled: 33
Confidence: 33.6%
Identity: 9%
Fold: SAM domain-like
Superfamily: RuvA domain 2-like
Family: Hef domain-like

Phyre2

PDB 2aq0 chain A domain 1

3D model

Region: 449 - 481
Aligned: 33
Modelled: 33
Confidence: 31.8%
Identity: 9%
Fold: SAM domain-like
Superfamily: RuvA domain 2-like
Family: Hef domain-like

Phyre2

PDB 1vqz chain A domain 1

3D model

Region: 406 - 482
Aligned: 69
Modelled: 77
Confidence: 24.8%
Identity: 10%
Fold: SufE/NifU
Superfamily: SufE/NifU
Family: SP1160 C-terminal domain-like

Phyre2

PDB 3cw1 chain D

3D model

Region: 404 - 424
Aligned: 21
Modelled: 21
Confidence: 19.5%
Identity: 19%
PDB header:splicing
Chain: D: PDB Molecule:small nuclear ribonucleoprotein sm d3;
PDBTitle: crystal structure of human spliceosomal u1 snrnp

Phyre2

PDB 1b34 chain A

3D model

Region: 404 - 424
Aligned: 21
Modelled: 21
Confidence: 17.8%
Identity: 19%
PDB header:rna binding protein
Chain: A: PDB Molecule:protein (small nuclear ribonucleoprotein sm d1);
PDBTitle: crystal structure of the d1d2 sub-complex from the human snrnp core2 domain

Phyre2

PDB 1b34 chain A

3D model

Region: 404 - 424
Aligned: 21
Modelled: 21
Confidence: 17.8%
Identity: 19%
Fold: Sm-like fold
Superfamily: Sm-like ribonucleoproteins
Family: Sm motif of small nuclear ribonucleoproteins, SNRNP

Phyre2

PDB 1i8f chain A

3D model

Region: 400 - 424
Aligned: 25
Modelled: 25
Confidence: 16.9%
Identity: 8%
Fold: Sm-like fold
Superfamily: Sm-like ribonucleoproteins
Family: Sm motif of small nuclear ribonucleoproteins, SNRNP

Phyre2

PDB 3cw1 chain 5

3D model

Region: 403 - 424
Aligned: 22
Modelled: 22
Confidence: 15.9%
Identity: 9%
PDB header:splicing
Chain: 5: PDB Molecule:small nuclear ribonucleoprotein g;
PDB Fragment:residues 1-215; PDBTitle: crystal structure of human spliceosomal u1 snrnp

Phyre2

PDB 2jfg chain A domain 3

3D model

Region: 177 - 198
Aligned: 22
Modelled: 22
Confidence: 15.9%
Identity: 27%
Fold: Ribokinase-like
Superfamily: MurD-like peptide ligases, catalytic domain
Family: MurCDEF

Phyre2

PDB 2fwk chain B

3D model

Region: 404 - 424
Aligned: 21
Modelled: 21
Confidence: 15.9%
Identity: 19%
PDB header:dna binding protein
Chain: B: PDB Molecule:u6 snrna-associated sm-like protein lsm5;
PDBTitle: crystal structure of cryptosporidium parvum u6 snrna-associated sm-2 like protein lsm5

Phyre2

PDB 1d3b chain A

3D model

Region: 404 - 424
Aligned: 21
Modelled: 21
Confidence: 15.1%
Identity: 19%
Fold: Sm-like fold
Superfamily: Sm-like ribonucleoproteins
Family: Sm motif of small nuclear ribonucleoproteins, SNRNP

Phyre2

PDB 1j6u chain A domain 3

3D model

Region: 177 - 199
Aligned: 23
Modelled: 23
Confidence: 15.1%
Identity: 17%
Fold: Ribokinase-like
Superfamily: MurD-like peptide ligases, catalytic domain
Family: MurCDEF

Phyre2

PDB 3swn chain T

3D model

Region: 400 - 424
Aligned: 25
Modelled: 25
Confidence: 15.0%
Identity: 8%
PDB header:rna binding protein
Chain: T: PDB Molecule:u6 snrna-associated sm-like protein lsm6;
PDBTitle: structure of the lsm657 complex: an assembly intermediate of the lsm12 7 and lsm2 8 rings

Phyre2

PDB 3bw1 chain A

3D model

Region: 400 - 424
Aligned: 25
Modelled: 25
Confidence: 15.0%
Identity: 4%
PDB header:rna binding protein
Chain: A: PDB Molecule:u6 snrna-associated sm-like protein lsm3;
PDBTitle: crystal structure of homomeric yeast lsm3 exhibiting novel octameric2 ring organisation

Phyre2

PDB 3pgw chain Q

3D model

Region: 403 - 424
Aligned: 22
Modelled: 22
Confidence: 14.5%
Identity: 18%
PDB header:splicing/dna/rna
Chain: Q: PDB Molecule:sm b;
PDBTitle: crystal structure of human u1 snrnp

Phyre2

PDB 1i4k chain 1

3D model

Region: 403 - 424
Aligned: 22
Modelled: 22
Confidence: 14.4%
Identity: 14%
Fold: Sm-like fold
Superfamily: Sm-like ribonucleoproteins
Family: Sm motif of small nuclear ribonucleoproteins, SNRNP

Phyre2

PDB 1mgq chain A

3D model

Region: 403 - 424
Aligned: 22
Modelled: 22
Confidence: 13.1%
Identity: 9%
Fold: Sm-like fold
Superfamily: Sm-like ribonucleoproteins
Family: Sm motif of small nuclear ribonucleoproteins, SNRNP

Phyre2

PDB 3cw1 chain A

3D model

Region: 403 - 424
Aligned: 22
Modelled: 22
Confidence: 13.1%
Identity: 18%
PDB header:splicing
Chain: A: PDB Molecule:small nuclear ribonucleoprotein-associated proteins b and
PDBTitle: crystal structure of human spliceosomal u1 snrnp

Phyre2
1

d1szqa_
2

c2hp0A_
3

d2a1ja1
4

d2aq0a1
5

d1vqza1
6

c3cw1D_
7

c1b34A_
8

d1b34a_
9

d1i8fa_
10

c3cw15_
11

d2jfga3
12

c2fwkB_
13

d1d3ba_
14

d1j6ua3
15

c3swnT_
16

c3bw1A_
17

c3pgwQ_
18

d1i4k1_
19

d1mgqa_
20

c3cw1A_
21



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67






Detailed template information 

#
Template Alignment Coverage3D Model Confidence
% i.d. Template Information
1d1szqa_



100.0 97 Fold:2-methylcitrate dehydratase PrpD
Superfamily:2-methylcitrate dehydratase PrpD
Family:2-methylcitrate dehydratase PrpD
2c2hp0A_



100.0 16 PDB header:isomerase
Chain: A: PDB Molecule:ids-epimerase;
PDBTitle: crystal structure of iminodisuccinate epimerase
3d2a1ja1



33.6 9 Fold:SAM domain-like
Superfamily:RuvA domain 2-like
Family:Hef domain-like
4d2aq0a1



31.8 9 Fold:SAM domain-like
Superfamily:RuvA domain 2-like
Family:Hef domain-like
5d1vqza1



24.8 10 Fold:SufE/NifU
Superfamily:SufE/NifU
Family:SP1160 C-terminal domain-like
6c3cw1D_



19.5 19 PDB header:splicing
Chain: D: PDB Molecule:small nuclear ribonucleoprotein sm d3;
PDBTitle: crystal structure of human spliceosomal u1 snrnp
7c1b34A_



17.8 19 PDB header:rna binding protein
Chain: A: PDB Molecule:protein (small nuclear ribonucleoprotein sm d1);
PDBTitle: crystal structure of the d1d2 sub-complex from the human snrnp core2 domain
8d1b34a_



17.8 19 Fold:Sm-like fold
Superfamily:Sm-like ribonucleoproteins
Family:Sm motif of small nuclear ribonucleoproteins, SNRNP
9d1i8fa_



16.9 8 Fold:Sm-like fold
Superfamily:Sm-like ribonucleoproteins
Family:Sm motif of small nuclear ribonucleoproteins, SNRNP
10c3cw15_



15.9 9 PDB header:splicing
Chain: 5: PDB Molecule:small nuclear ribonucleoprotein g;
PDB Fragment:residues 1-215; PDBTitle: crystal structure of human spliceosomal u1 snrnp
11d2jfga3



15.9 27 Fold:Ribokinase-like
Superfamily:MurD-like peptide ligases, catalytic domain
Family:MurCDEF
12c2fwkB_



15.9 19 PDB header:dna binding protein
Chain: B: PDB Molecule:u6 snrna-associated sm-like protein lsm5;
PDBTitle: crystal structure of cryptosporidium parvum u6 snrna-associated sm-2 like protein lsm5
13d1d3ba_



15.1 19 Fold:Sm-like fold
Superfamily:Sm-like ribonucleoproteins
Family:Sm motif of small nuclear ribonucleoproteins, SNRNP
14d1j6ua3



15.1 17 Fold:Ribokinase-like
Superfamily:MurD-like peptide ligases, catalytic domain
Family:MurCDEF
15c3swnT_



15.0 8 PDB header:rna binding protein
Chain: T: PDB Molecule:u6 snrna-associated sm-like protein lsm6;
PDBTitle: structure of the lsm657 complex: an assembly intermediate of the lsm12 7 and lsm2 8 rings
16c3bw1A_



15.0 4 PDB header:rna binding protein
Chain: A: PDB Molecule:u6 snrna-associated sm-like protein lsm3;
PDBTitle: crystal structure of homomeric yeast lsm3 exhibiting novel octameric2 ring organisation
17c3pgwQ_



14.5 18 PDB header:splicing/dna/rna
Chain: Q: PDB Molecule:sm b;
PDBTitle: crystal structure of human u1 snrnp
18d1i4k1_



14.4 14 Fold:Sm-like fold
Superfamily:Sm-like ribonucleoproteins
Family:Sm motif of small nuclear ribonucleoproteins, SNRNP
19d1mgqa_



13.1 9 Fold:Sm-like fold
Superfamily:Sm-like ribonucleoproteins
Family:Sm motif of small nuclear ribonucleoproteins, SNRNP
20c3cw1A_



13.1 18 PDB header:splicing
Chain: A: PDB Molecule:small nuclear ribonucleoprotein-associated proteins b and
PDBTitle: crystal structure of human spliceosomal u1 snrnp
21c3pgwB_



not modelled 12.5 18 PDB header:splicing/dna/rna
Chain: B: PDB Molecule:sm b;
PDBTitle: crystal structure of human u1 snrnp
22d1pzna1



not modelled 12.5 6 Fold:SAM domain-like
Superfamily:Rad51 N-terminal domain-like
Family:DNA repair protein Rad51, N-terminal domain
23d1e6va2



not modelled 12.4 23 Fold:Ferredoxin-like
Superfamily:Methyl-coenzyme M reductase subunits
Family:Methyl-coenzyme M reductase alpha and beta chain N-terminal domain
24d1jbma_



not modelled 12.2 8 Fold:Sm-like fold
Superfamily:Sm-like ribonucleoproteins
Family:Sm motif of small nuclear ribonucleoproteins, SNRNP
25c3swnA_



not modelled 12.0 10 PDB header:rna binding protein
Chain: A: PDB Molecule:u6 snrna-associated sm-like protein lsm5;
PDBTitle: structure of the lsm657 complex: an assembly intermediate of the lsm12 7 and lsm2 8 rings
26d2fwka1



not modelled 12.0 23 Fold:Sm-like fold
Superfamily:Sm-like ribonucleoproteins
Family:Sm motif of small nuclear ribonucleoproteins, SNRNP
27d1h641_



not modelled 11.7 16 Fold:Sm-like fold
Superfamily:Sm-like ribonucleoproteins
Family:Sm motif of small nuclear ribonucleoproteins, SNRNP
28d1d3bb_



not modelled 11.5 18 Fold:Sm-like fold
Superfamily:Sm-like ribonucleoproteins
Family:Sm motif of small nuclear ribonucleoproteins, SNRNP
29c3cw1Z_



not modelled 11.3 10 PDB header:splicing
Chain: Z: PDB Molecule:small nuclear ribonucleoprotein f;
PDBTitle: crystal structure of human spliceosomal u1 snrnp
30d1kfta_



not modelled 11.3 16 Fold:SAM domain-like
Superfamily:RuvA domain 2-like
Family:Excinuclease UvrC C-terminal domain
31c1kftA_



not modelled 11.3 16 PDB header:dna binding protein
Chain: A: PDB Molecule:excinuclease abc subunit c;
PDBTitle: solution structure of the c-terminal domain of uvrc from e-2 coli
32d2bgwa1



not modelled 10.7 22 Fold:SAM domain-like
Superfamily:RuvA domain 2-like
Family:Hef domain-like
33d1m5q1_



not modelled 10.7 10 Fold:Sm-like fold
Superfamily:Sm-like ribonucleoproteins
Family:Sm motif of small nuclear ribonucleoproteins, SNRNP
34d1th7a1



not modelled 10.6 9 Fold:Sm-like fold
Superfamily:Sm-like ribonucleoproteins
Family:Sm motif of small nuclear ribonucleoproteins, SNRNP
35d2i1qa1



not modelled 10.4 16 Fold:SAM domain-like
Superfamily:Rad51 N-terminal domain-like
Family:DNA repair protein Rad51, N-terminal domain
36c3swnC_



not modelled 10.3 18 PDB header:rna binding protein
Chain: C: PDB Molecule:u6 snrna-associated sm-like protein lsm7;
PDBTitle: structure of the lsm657 complex: an assembly intermediate of the lsm12 7 and lsm2 8 rings
37d1d3bl_



not modelled 10.2 18 Fold:Sm-like fold
Superfamily:Sm-like ribonucleoproteins
Family:Sm motif of small nuclear ribonucleoproteins, SNRNP
38d1kn0a_



not modelled 10.0 21 Fold:dsRBD-like
Superfamily:dsRNA-binding domain-like
Family:The homologous-pairing domain of Rad52 recombinase
39c3iacA_



not modelled 9.7 6 PDB header:isomerase
Chain: A: PDB Molecule:glucuronate isomerase;
PDBTitle: 2.2 angstrom crystal structure of glucuronate isomerase from2 salmonella typhimurium.
40d1gg4a4



not modelled 8.8 18 Fold:Ribokinase-like
Superfamily:MurD-like peptide ligases, catalytic domain
Family:MurCDEF
41d1bjna_



not modelled 8.6 13 Fold:PLP-dependent transferase-like
Superfamily:PLP-dependent transferases
Family:GABA-aminotransferase-like
42d2a1jb1



not modelled 8.1 9 Fold:SAM domain-like
Superfamily:RuvA domain 2-like
Family:Hef domain-like
43d1e8ca3



not modelled 8.1 14 Fold:Ribokinase-like
Superfamily:MurD-like peptide ligases, catalytic domain
Family:MurCDEF
44c1y7wB_



not modelled 8.1 8 PDB header:lyase
Chain: B: PDB Molecule:halotolerant alpha-type carbonic anhydrase (dca ii);
PDBTitle: crystal structure of a halotolerant carbonic anhydrase from dunaliella2 salina
45c2e12B_



not modelled 8.0 25 PDB header:translation
Chain: B: PDB Molecule:hypothetical protein xcc3642;
PDBTitle: the crystal structure of xc5848 from xanthomonas campestris2 adopting a novel variant of sm-like motif
46d1dw0a_



not modelled 7.9 13 Fold:Cytochrome c
Superfamily:Cytochrome c
Family:monodomain cytochrome c
47d1o5za2



not modelled 7.5 19 Fold:Ribokinase-like
Superfamily:MurD-like peptide ligases, catalytic domain
Family:Folylpolyglutamate synthetase
48c1b34B_



not modelled 7.5 5 PDB header:rna binding protein
Chain: B: PDB Molecule:protein (small nuclear ribonucleoprotein sm d2);
PDBTitle: crystal structure of the d1d2 sub-complex from the human snrnp core2 domain
49d1b34b_



not modelled 7.5 5 Fold:Sm-like fold
Superfamily:Sm-like ribonucleoproteins
Family:Sm motif of small nuclear ribonucleoproteins, SNRNP
50c3k8rA_



not modelled 7.4 10 PDB header:structural genomics, unknown function
Chain: A: PDB Molecule:uncharacterized protein;
PDBTitle: crystal structure of protein of unknown function (yp_427503.1) from2 rhodospirillum rubrum atcc 11170 at 2.75 a resolution
51d1vlfm2



not modelled 7.1 7 Fold:Formate dehydrogenase/DMSO reductase, domains 1-3
Superfamily:Formate dehydrogenase/DMSO reductase, domains 1-3
Family:Formate dehydrogenase/DMSO reductase, domains 1-3
52c2k1vB_



not modelled 6.8 25 PDB header:hormone
Chain: B: PDB Molecule:relaxin-3;
PDBTitle: r3/i5 relaxin chimera
53c1h2iG_



not modelled 6.7 21 PDB header:dna-binding protein
Chain: G: PDB Molecule:dna repair protein rad52 homolog;
PDBTitle: human rad52 protein, n-terminal domain
54c2dfwA_



not modelled 6.6 14 PDB header:hydrolase
Chain: A: PDB Molecule:salt-tolerant glutaminase;
PDBTitle: crystal structure of a major fragment of the salt-tolerant2 glutaminase from micrococcus luteus k-3
55d1vp7b_



not modelled 6.5 11 Fold:Spectrin repeat-like
Superfamily:XseB-like
Family:XseB-like
56d1p3da3



not modelled 6.5 30 Fold:Ribokinase-like
Superfamily:MurD-like peptide ligases, catalytic domain
Family:MurCDEF
57d2f02a1



not modelled 6.3 10 Fold:Ribokinase-like
Superfamily:Ribokinase-like
Family:Ribokinase-like
58c1vqzA_



not modelled 6.1 9 PDB header:ligase
Chain: A: PDB Molecule:lipoate-protein ligase, putative;
PDBTitle: crystal structure of a putative lipoate-protein ligase a (sp_1160)2 from streptococcus pneumoniae tigr4 at 1.99 a resolution
59d1x2ia1



not modelled 6.0 12 Fold:SAM domain-like
Superfamily:RuvA domain 2-like
Family:Hef domain-like
60d1sg5a1



not modelled 6.0 28 Fold:Rof/RNase P subunit-like
Superfamily:Rof/RNase P subunit-like
Family:Rof-like
61c1xhoB_



not modelled 5.9 17 PDB header:structural genomics, unknown function
Chain: B: PDB Molecule:chorismate mutase;
PDBTitle: chorismate mutase from clostridium thermocellum cth-682
62d1xhoa_



not modelled 5.9 17 Fold:Bacillus chorismate mutase-like
Superfamily:YjgF-like
Family:Chorismate mutase
63d1vp7a_



not modelled 5.8 11 Fold:Spectrin repeat-like
Superfamily:XseB-like
Family:XseB-like
64d1gu2a_



not modelled 5.8 21 Fold:Cytochrome c
Superfamily:Cytochrome c
Family:monodomain cytochrome c
65d1ju3a2



not modelled 5.8 19 Fold:alpha/beta-Hydrolases
Superfamily:alpha/beta-Hydrolases
Family:PepX catalytic domain-like
66c3eagA_



not modelled 5.4 14 PDB header:ligase
Chain: A: PDB Molecule:udp-n-acetylmuramate:l-alanyl-gamma-d-glutamyl-meso-
PDBTitle: the crystal structure of udp-n-acetylmuramate:l-alanyl-gamma-d-2 glutamyl-meso-diaminopimelate ligase (mpl) from neisseria3 meningitides
67d1fnja_



not modelled 5.1 10 Fold:Bacillus chorismate mutase-like
Superfamily:YjgF-like
Family:Chorismate mutase

Binding site prediction 

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

Transmembrane helix prediction 

Transmembrane helices have been predicted in your sequence to adopt the topology shown below



Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
 
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
 
© Structural Bioinformatics Group
Imperial College London
Lawrence Kelley, Benjamin Jefferys
Disclaimer
Terms and Conditions
Component software
Template detection: HHpred 1.51
Secondary structure prediction: Psi-pred 2.5
Disorder prediction: Disopred 2.4
Transmembrane prediction: Memsat_SVM
Multi-template modelling and ab initio: Poing 1.0