Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	K	S	V	R	Y	L	I	G	L	F	A	F	I	A	C	Y	Y	L	L	P	I	S	T	R	L	L	W	Q	P	D	E	T	R	Y	A	E	I	S	R	E	M	L	A	S	G	D	W	I	V	P	H	L	L	G	L	R	Y	F	E
Secondary structure
SS confidence
Disorder		?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		K	P	I	A	G	Y	W	I	N	S	I	G	Q	W	L	F	G	A	N	N	F	G	V	R	A	G	V	I	F	A	T	L	L	T	A	A	L	V	T	W	F	T	L	R	L	W	R	D	K	R	L	A	L	L	A	T	V	I	Y	L
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		S	L	F	I	V	Y	A	I	G	T	Y	A	V	L	D	P	F	I	A	F	W	L	V	A	G	M	C	S	F	W	L	A	M	Q	A	Q	T	W	K	G	K	S	A	G	F	L	L	L	G	I	T	C	G	M	G	V	M	T	K	G
Secondary structure
SS confidence
Disorder																																			?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		F	L	A	L	A	V	P	V	L	S	V	L	P	W	V	A	T	Q	K	R	W	K	D	L	F	I	Y	G	W	L	A	V	I	S	C	V	L	T	V	L	P	W	G	L	A	I	A	Q	R	E	P	N	F	W	H	Y	F	F	W	V
Secondary structure
SS confidence
Disorder																						?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		E	H	I	Q	R	F	A	L	D	D	A	Q	H	R	A	P	F	W	Y	Y	V	P	V	I	I	A	G	S	L	P	W	L	G	L	L	P	G	A	L	Y	T	G	W	K	N	R	K	H	S	A	T	V	Y	L	L	S	W	T	I	M
Secondary structure
SS confidence
Disorder									?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		P	L	L	F	F	S	V	A	K	G	K	L	P	T	Y	I	L	S	C	F	A	S	L	A	M	L	M	A	H	Y	A	L	L	A	A	K	N	N	P	L	A	L	R	I	N	G	W	I	N	I	A	F	G	V	T	G	I	I	A	T
Secondary structure
SS confidence
Disorder																																					?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420
Sequence		F	V	V	S	P	W	G	P	M	N	T	P	V	W	Q	T	F	E	S	Y	K	V	F	C	A	W	S	I	F	S	L	W	A	F	F	G	W	Y	T	L	T	N	V	E	K	T	W	P	F	A	A	L	C	P	L	G	L	A	L	L
Secondary structure
SS confidence
Disorder									?			?	?	?																											?			?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.	.	.	.	.	.	.	460	.	.	.	.	.	.	.	.	.	470	.	.	.	.	.	.	.	.	.	480
Sequence		V	G	F	S	I	P	D	R	V	M	E	G	K	H	P	Q	F	F	V	E	M	T	Q	E	S	L	Q	P	S	R	Y	I	L	T	D	S	V	G	V	A	A	G	L	A	W	S	L	Q	R	D	D	I	I	M	Y	R	Q	T	G	E
Secondary structure
SS confidence
Disorder										?	?	?	?	?	?	?									?	?	?	?											?
Disorder confidence
		.	.	.	.	.	.	.	.	.	490	.	.	.	.	.	.	.	.	.	500	.	.	.	.	.	.	.	.	.	510	.	.	.	.	.	.	.	.	.	520	.	.	.	.	.	.	.	.	.	530	.	.	.	.	.	.	.	.	.	540
Sequence		L	K	Y	G	L	N	Y	P	D	A	K	G	R	F	V	S	G	D	E	F	A	N	W	L	N	Q	H	R	Q	E	G	I	I	T	L	V	L	S	V	D	R	D	E	D	I	N	S	L	A	I	P	P	A	D	A	I	D	R	Q	E
Secondary structure
SS confidence
Disorder		?	?	?	?		?				?	?																									?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	550
Sequence		R	L	V	L	I	Q	Y	R	P	K
Secondary structure
SS confidence
Disorder									?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 3rce chain A Region: 2 - 383 Aligned: 365 Modelled: 380 Confidence: 99.8% Identity: 13% PDB header:transferase/peptide Chain: A: PDB Molecule:oligosaccharide transferase to n-glycosylate proteins; PDBTitle: bacterial oligosaccharyltransferase pglb Phyre2 PDB 3erv chain A Region: 439 - 521 Aligned: 81 Modelled: 82 Confidence: 48.0% Identity: 11% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:putative c39-like peptidase; PDBTitle: crystal structure of an putative c39-like peptidase from2 bacillus anthracis Phyre2 PDB 1q59 chain A Region: 29 - 48 Aligned: 20 Modelled: 20 Confidence: 11.6% Identity: 10% Fold: Toxins' membrane translocation domains Superfamily: Bcl-2 inhibitors of programmed cell death Family: Bcl-2 inhibitors of programmed cell death Phyre2 PDB 2qml chain A Region: 427 - 506 Aligned: 74 Modelled: 80 Confidence: 10.7% Identity: 3% PDB header:unknown function Chain: A: PDB Molecule:bh2621 protein; PDBTitle: crystal structure of an uncharacterized protein (bh2621) from bacillus2 halodurans at 1.55 a resolution Phyre2 PDB 1un2 chain A Region: 495 - 544 Aligned: 45 Modelled: 50 Confidence: 9.5% Identity: 13% Fold: Thioredoxin fold Superfamily: Thioredoxin-like Family: DsbA-like Phyre2 PDB 2l42 chain A Region: 455 - 510 Aligned: 39 Modelled: 39 Confidence: 8.9% Identity: 23% PDB header:protein binding Chain: A: PDB Molecule:dna-binding protein rap1; PDBTitle: the solution structure of rap1 brct domain from saccharomyces2 cerevisiae Phyre2 PDB 2zme chain B Region: 494 - 544 Aligned: 51 Modelled: 51 Confidence: 7.4% Identity: 16% PDB header:protein transport Chain: B: PDB Molecule:vacuolar protein-sorting-associated protein 36; PDBTitle: integrated structural and functional model of the human escrt-ii2 complex Phyre2 PDB 1gso chain A domain 2 Region: 450 - 531 Aligned: 81 Modelled: 82 Confidence: 7.2% Identity: 12% Fold: PreATP-grasp domain Superfamily: PreATP-grasp domain Family: BC N-terminal domain-like Phyre2 PDB 2jm6 chain B domain 1 Region: 29 - 48 Aligned: 20 Modelled: 20 Confidence: 7.1% Identity: 15% Fold: Toxins' membrane translocation domains Superfamily: Bcl-2 inhibitors of programmed cell death Family: Bcl-2 inhibitors of programmed cell death Phyre2 PDB 1vjq chain B Region: 507 - 540 Aligned: 33 Modelled: 34 Confidence: 7.1% Identity: 15% PDB header:structural genomics, de novo protein Chain: B: PDB Molecule:designed protein; PDBTitle: designed protein based on backbone conformation of2 procarboxypeptidase-a (1aye) with sidechains chosen for maximal3 predicted stability. Phyre2 PDB 2i09 chain A domain 2 Region: 34 - 48 Aligned: 15 Modelled: 15 Confidence: 7.0% Identity: 20% Fold: DeoB insert domain-like Superfamily: DeoB insert domain-like Family: DeoB insert domain-like Phyre2 PDB 1js8 chain A Region: 482 - 523 Aligned: 42 Modelled: 42 Confidence: 7.0% Identity: 14% PDB header:oxygen storage/transport Chain: A: PDB Molecule:hemocyanin; PDBTitle: structure of a functional unit from octopus hemocyanin Phyre2 PDB 1hqt chain A Region: 462 - 536 Aligned: 74 Modelled: 75 Confidence: 6.9% Identity: 18% Fold: TIM beta/alpha-barrel Superfamily: NAD(P)-linked oxidoreductase Family: Aldo-keto reductases (NADP) Phyre2 PDB 3cht chain A Region: 31 - 45 Aligned: 15 Modelled: 15 Confidence: 6.6% Identity: 33% PDB header:oxidoreductase Chain: A: PDB Molecule:p-aminobenzoate n-oxygenase; PDBTitle: crystal structure of di-iron aurf with partially bound ligand Phyre2 PDB 1qle chain B Region: 446 - 513 Aligned: 66 Modelled: 68 Confidence: 6.6% Identity: 21% PDB header:oxidoreductase/immune system Chain: B: PDB Molecule:cytochrome c oxidase polypeptide ii; PDBTitle: cryo-structure of the paracoccus denitrificans four-subunit2 cytochrome c oxidase in the completely oxidized state3 complexed with an antibody fv fragment Phyre2 PDB 1ar1 chain B Region: 446 - 513 Aligned: 66 Modelled: 68 Confidence: 6.6% Identity: 21% PDB header:complex (oxidoreductase/antibody) Chain: B: PDB Molecule:cytochrome c oxidase; PDBTitle: structure at 2.7 angstrom resolution of the paracoccus2 denitrificans two-subunit cytochrome c oxidase complexed3 with an antibody fv fragment Phyre2 PDB 2dpl chain A Region: 433 - 467 Aligned: 35 Modelled: 35 Confidence: 6.2% Identity: 26% PDB header:ligase Chain: A: PDB Molecule:gmp synthase [glutamine-hydrolyzing] subunit b; PDBTitle: crystal structure of the gmp synthase from pyrococcus horikoshii ot3 Phyre2 PDB 2oqz chain A domain 1 Region: 464 - 543 Aligned: 80 Modelled: 80 Confidence: 6.1% Identity: 13% Fold: Sortase Superfamily: Sortase Family: Sortase Phyre2 PDB 3t6o chain A Region: 467 - 530 Aligned: 57 Modelled: 64 Confidence: 6.0% Identity: 11% PDB header:transport protein Chain: A: PDB Molecule:sulfate transporter/antisigma-factor antagonist stas; PDBTitle: the structure of an anti-sigma-factor antagonist (stas) domain protein2 from planctomyces limnophilus. Phyre2 PDB 2xa0 chain A Region: 29 - 48 Aligned: 20 Modelled: 20 Confidence: 6.0% Identity: 25% PDB header:apoptosis Chain: A: PDB Molecule:apoptosis regulator bcl-2; PDBTitle: crystal structure of bcl-2 in complex with a bax bh32 peptide Phyre2 1 c3rceA_ 2 c3ervA_ 3 d1q59a_ 4 c2qmlA_ 5 d1un2a_ 6 c2l42A_ 7 c2zmeB_ 8 d1gsoa2 9 d2jm6b1 10 c1vjqB_ 11 d2i09a2 12 c1js8A_ 13 d1hqta_ 14 c3chtA_ 15 c1qleB_ 16 c1ar1B_ 17 c2dplA_ 18 d2oqza1 19 c3t6oA_ 20 c2xa0A_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 c3rceA_ 99.8 13 PDB header:transferase/peptide Chain: A: PDB Molecule:oligosaccharide transferase to n-glycosylate proteins; PDBTitle: bacterial oligosaccharyltransferase pglb 2 c3ervA_ 48.0 11 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:putative c39-like peptidase; PDBTitle: crystal structure of an putative c39-like peptidase from2 bacillus anthracis 3 d1q59a_ 11.6 10 Fold:Toxins' membrane translocation domains Superfamily:Bcl-2 inhibitors of programmed cell death Family:Bcl-2 inhibitors of programmed cell death 4 c2qmlA_ 10.7 3 PDB header:unknown function Chain: A: PDB Molecule:bh2621 protein; PDBTitle: crystal structure of an uncharacterized protein (bh2621) from bacillus2 halodurans at 1.55 a resolution 5 d1un2a_ 9.5 13 Fold:Thioredoxin fold Superfamily:Thioredoxin-like Family:DsbA-like 6 c2l42A_ 8.9 23 PDB header:protein binding Chain: A: PDB Molecule:dna-binding protein rap1; PDBTitle: the solution structure of rap1 brct domain from saccharomyces2 cerevisiae 7 c2zmeB_ 7.4 16 PDB header:protein transport Chain: B: PDB Molecule:vacuolar protein-sorting-associated protein 36; PDBTitle: integrated structural and functional model of the human escrt-ii2 complex 8 d1gsoa2 7.2 12 Fold:PreATP-grasp domain Superfamily:PreATP-grasp domain Family:BC N-terminal domain-like 9 d2jm6b1 7.1 15 Fold:Toxins' membrane translocation domains Superfamily:Bcl-2 inhibitors of programmed cell death Family:Bcl-2 inhibitors of programmed cell death 10 c1vjqB_ 7.1 15 PDB header:structural genomics, de novo protein Chain: B: PDB Molecule:designed protein; PDBTitle: designed protein based on backbone conformation of2 procarboxypeptidase-a (1aye) with sidechains chosen for maximal3 predicted stability. 11 d2i09a2 7.0 20 Fold:DeoB insert domain-like Superfamily:DeoB insert domain-like Family:DeoB insert domain-like 12 c1js8A_ 7.0 14 PDB header:oxygen storage/transport Chain: A: PDB Molecule:hemocyanin; PDBTitle: structure of a functional unit from octopus hemocyanin 13 d1hqta_ 6.9 18 Fold:TIM beta/alpha-barrel Superfamily:NAD(P)-linked oxidoreductase Family:Aldo-keto reductases (NADP) 14 c3chtA_ 6.6 33 PDB header:oxidoreductase Chain: A: PDB Molecule:p-aminobenzoate n-oxygenase; PDBTitle: crystal structure of di-iron aurf with partially bound ligand 15 c1qleB_ 6.6 21 PDB header:oxidoreductase/immune system Chain: B: PDB Molecule:cytochrome c oxidase polypeptide ii; PDBTitle: cryo-structure of the paracoccus denitrificans four-subunit2 cytochrome c oxidase in the completely oxidized state3 complexed with an antibody fv fragment 16 c1ar1B_ 6.6 21 PDB header:complex (oxidoreductase/antibody) Chain: B: PDB Molecule:cytochrome c oxidase; PDBTitle: structure at 2.7 angstrom resolution of the paracoccus2 denitrificans two-subunit cytochrome c oxidase complexed3 with an antibody fv fragment 17 c2dplA_ 6.2 26 PDB header:ligase Chain: A: PDB Molecule:gmp synthase [glutamine-hydrolyzing] subunit b; PDBTitle: crystal structure of the gmp synthase from pyrococcus horikoshii ot3 18 d2oqza1 6.1 13 Fold:Sortase Superfamily:Sortase Family:Sortase 19 c3t6oA_ 6.0 11 PDB header:transport protein Chain: A: PDB Molecule:sulfate transporter/antisigma-factor antagonist stas; PDBTitle: the structure of an anti-sigma-factor antagonist (stas) domain protein2 from planctomyces limnophilus. 20 c2xa0A_ 6.0 25 PDB header:apoptosis Chain: A: PDB Molecule:apoptosis regulator bcl-2; PDBTitle: crystal structure of bcl-2 in complex with a bax bh32 peptide 21 c3c67B_ not modelled 6.0 10 PDB header:hydrolase Chain: B: PDB Molecule:uncharacterized protein ygjk; PDBTitle: escherichia coli k12 ygjk in a complexed with glucose 22 d1wdpa1 not modelled 5.9 48 Fold:TIM beta/alpha-barrel Superfamily:(Trans)glycosidases Family:Amylase, catalytic domain 23 d1b1ya_ not modelled 5.8 48 Fold:TIM beta/alpha-barrel Superfamily:(Trans)glycosidases Family:Amylase, catalytic domain 24 d1o0la_ not modelled 5.7 25 Fold:Toxins' membrane translocation domains Superfamily:Bcl-2 inhibitors of programmed cell death Family:Bcl-2 inhibitors of programmed cell death 25 d2fcia1 not modelled 5.7 9 Fold:SH2-like Superfamily:SH2 domain Family:SH2 domain 26 d1fa2a_ not modelled 5.6 48 Fold:TIM beta/alpha-barrel Superfamily:(Trans)glycosidases Family:Amylase, catalytic domain 27 d2nwua1 not modelled 5.6 29 Fold:RL5-like Superfamily:RL5-like Family:SSO1042-like 28 c2yv6A_ not modelled 5.5 20 PDB header:apoptosis Chain: A: PDB Molecule:bcl-2 homologous antagonist/killer; PDBTitle: crystal structure of human bcl-2 family protein bak 29 c2o2fA_ not modelled 5.5 25 PDB header:apoptosis Chain: A: PDB Molecule:apoptosis regulator bcl-2; PDBTitle: solution structure of the anti-apoptotic protein bcl-2 in2 complex with an acyl-sulfonamide-based ligand 30 c3k8uA_ not modelled 5.4 23 PDB header:hydrolase Chain: A: PDB Molecule:putative abc transporter, atp-binding protein PDBTitle: crystal structure of the peptidase domain of streptococcus2 coma, a bi-functional abc transporter involved in quorum3 sensing pathway 31 d1pq1a_ not modelled 5.2 15 Fold:Toxins' membrane translocation domains Superfamily:Bcl-2 inhibitors of programmed cell death Family:Bcl-2 inhibitors of programmed cell death 32 c2vofA_ not modelled 5.2 13 PDB header:apoptosis Chain: A: PDB Molecule:bcl-2-related protein a1; PDBTitle: structure of mouse a1 bound to the puma bh3-domain 33 d1qwza_ not modelled 5.2 13 Fold:Sortase Superfamily:Sortase Family:Sortase 34 d1piia1 not modelled 5.1 11 Fold:TIM beta/alpha-barrel Superfamily:Ribulose-phoshate binding barrel Family:Tryptophan biosynthesis enzymes

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0