Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	S	H	I	T	T	E	D	P	A	T	L	R	L	P	F	K	E	K	L	S	Y	G	I	G	D	L	A	S	N	I	L	L	D	I	G	T	L	Y	L	L	K	F	Y	T	D	V	L	G	L	P	G	T	Y	G	G	I	I	F	L
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		I	S	K	F	F	T	A	F	T	D	M	G	T	G	I	M	L	D	S	R	R	K	I	G	P	K	G	K	F	R	P	F	I	L	Y	A	S	F	P	V	T	L	L	A	I	A	N	F	V	G	T	P	F	D	V	T	G	K	T	V
Secondary structure
SS confidence
Disorder																																																						?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		M	A	T	I	L	F	M	L	Y	G	L	F	F	S	M	M	N	C	S	Y	G	A	M	V	P	A	I	T	K	N	P	N	E	R	A	S	L	A	A	W	R	Q	G	G	A	T	L	G	L	L	L	C	T	V	G	F	V	P	V	M
Secondary structure
SS confidence
Disorder																														?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		N	L	I	E	G	N	Q	Q	L	G	Y	I	F	A	A	T	L	F	S	L	F	G	L	L	F	M	W	I	C	Y	S	G	V	K	E	R	Y	V	E	T	Q	P	A	N	P	A	Q	K	P	G	L	L	Q	S	F	R	A	I	A	G
Secondary structure
SS confidence
Disorder																																					?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		N	R	P	L	F	I	L	C	I	A	N	L	C	T	L	G	A	F	N	V	K	L	A	I	Q	V	Y	Y	T	Q	Y	V	L	N	D	P	I	L	L	S	Y	M	G	F	F	S	M	G	C	I	F	I	G	V	F	L	M	P	A	S
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		V	R	R	F	G	K	K	K	V	Y	I	G	G	L	L	I	W	V	L	G	D	L	L	N	Y	F	F	G	G	G	S	V	S	F	V	A	F	S	C	L	A	F	F	G	S	A	F	V	N	S	L	N	W	A	L	V	S	D	T	V
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420
Sequence		E	Y	G	E	W	R	T	G	V	R	S	E	G	T	V	Y	T	G	F	T	F	F	R	K	V	S	Q	A	L	A	G	F	F	P	G	W	M	L	T	Q	I	G	Y	V	P	N	V	A	Q	A	D	H	T	I	E	G	L	R	Q	L
Secondary structure
SS confidence
Disorder																																																?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.	.	.	.	.	.	.	460	.
Sequence		I	F	I	Y	P	S	A	L	A	V	V	T	I	V	A	M	G	C	F	Y	S	L	N	E	K	M	Y	V	R	I	V	E	E	I	E	A	R	K	R	T	A
Secondary structure
SS confidence
Disorder																																								?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 1pw4 chain A Region: 3 - 454 Aligned: 417 Modelled: 430 Confidence: 100.0% Identity: 11% Fold: MFS general substrate transporter Superfamily: MFS general substrate transporter Family: Glycerol-3-phosphate transporter Phyre2 PDB 1pv7 chain A Region: 12 - 455 Aligned: 413 Modelled: 422 Confidence: 100.0% Identity: 14% Fold: MFS general substrate transporter Superfamily: MFS general substrate transporter Family: LacY-like proton/sugar symporter Phyre2 PDB 2gfp chain A Region: 19 - 442 Aligned: 372 Modelled: 397 Confidence: 99.9% Identity: 11% PDB header:membrane protein Chain: A: PDB Molecule:multidrug resistance protein d; PDBTitle: structure of the multidrug transporter emrd from2 escherichia coli Phyre2 PDB 3o7p chain A Region: 17 - 443 Aligned: 391 Modelled: 405 Confidence: 99.9% Identity: 12% PDB header:transport protein Chain: A: PDB Molecule:l-fucose-proton symporter; PDBTitle: crystal structure of the e.coli fucose:proton symporter, fucp (n162a) Phyre2 PDB 2xut chain C Region: 24 - 431 Aligned: 389 Modelled: 401 Confidence: 99.9% Identity: 11% PDB header:transport protein Chain: C: PDB Molecule:proton/peptide symporter family protein; PDBTitle: crystal structure of a proton dependent oligopeptide (pot)2 family transporter. Phyre2 PDB 3qnq chain D Region: 385 - 456 Aligned: 72 Modelled: 72 Confidence: 60.1% Identity: 14% PDB header:membrane protein, transport protein Chain: D: PDB Molecule:pts system, cellobiose-specific iic component; PDBTitle: crystal structure of the transporter chbc, the iic component from the2 n,n'-diacetylchitobiose-specific phosphotransferase system Phyre2 PDB 1m46 chain B Region: 447 - 458 Aligned: 12 Modelled: 12 Confidence: 17.4% Identity: 25% PDB header:cell cycle protein Chain: B: PDB Molecule:iq4 motif from myo2p, a class v myosin; PDBTitle: crystal structure of mlc1p bound to iq4 of myo2p, a class v2 myosin Phyre2 PDB 2knc chain B Region: 417 - 460 Aligned: 44 Modelled: 44 Confidence: 15.7% Identity: 14% PDB header:cell adhesion Chain: B: PDB Molecule:integrin beta-3; PDBTitle: platelet integrin alfaiib-beta3 transmembrane-cytoplasmic2 heterocomplex Phyre2 PDB 2rdc chain A Region: 435 - 461 Aligned: 27 Modelled: 26 Confidence: 13.6% Identity: 15% PDB header:lipid binding protein Chain: A: PDB Molecule:uncharacterized protein; PDBTitle: crystal structure of a putative lipid binding protein (gsu0061) from2 geobacter sulfurreducens pca at 1.80 a resolution Phyre2 PDB 1l4a chain E Region: 444 - 460 Aligned: 17 Modelled: 17 Confidence: 13.4% Identity: 24% PDB header:endocytosis/exocytosis Chain: E: PDB Molecule:synaphin a; PDBTitle: x-ray structure of the neuronal complexin/snare complex2 from the squid loligo pealei Phyre2 PDB 1by0 chain A Region: 444 - 459 Aligned: 16 Modelled: 16 Confidence: 10.8% Identity: 13% PDB header:rna binding protein Chain: A: PDB Molecule:protein (hepatitis delta antigen); PDBTitle: n-terminal leucine-repeat region of hepatitis delta antigen Phyre2 PDB 3d9s chain B Region: 419 - 456 Aligned: 38 Modelled: 38 Confidence: 9.8% Identity: 16% PDB header:membrane protein Chain: B: PDB Molecule:aquaporin-5; PDBTitle: human aquaporin 5 (aqp5) - high resolution x-ray structure Phyre2 PDB 1sb0 chain A Region: 439 - 460 Aligned: 22 Modelled: 22 Confidence: 9.0% Identity: 27% Fold: Kix domain of CBP (creb binding protein) Superfamily: Kix domain of CBP (creb binding protein) Family: Kix domain of CBP (creb binding protein) Phyre2 PDB 2p0m chain A domain 1 Region: 444 - 460 Aligned: 17 Modelled: 17 Confidence: 8.9% Identity: 24% Fold: Lipoxigenase Superfamily: Lipoxigenase Family: Animal lipoxigenases Phyre2 PDB 2b5u chain A domain 2 Region: 441 - 461 Aligned: 21 Modelled: 21 Confidence: 7.9% Identity: 14% Fold: Cloacin translocation domain Superfamily: Cloacin translocation domain Family: Cloacin translocation domain Phyre2 PDB 2rdd chain B Region: 428 - 456 Aligned: 29 Modelled: 29 Confidence: 7.6% Identity: 10% PDB header:membrane protein/transport protein Chain: B: PDB Molecule:upf0092 membrane protein yajc; PDBTitle: x-ray crystal structure of acrb in complex with a novel2 transmembrane helix. Phyre2 PDB 3d3l chain B Region: 444 - 460 Aligned: 17 Modelled: 17 Confidence: 6.8% Identity: 24% PDB header:oxidoreductase Chain: B: PDB Molecule:arachidonate 12-lipoxygenase, 12s-type; PDBTitle: the 2.6 a crystal structure of the lipoxygenase domain of2 human arachidonate 12-lipoxygenase, 12s-type (casp target) Phyre2 PDB 2fnq chain B Region: 444 - 460 Aligned: 17 Modelled: 17 Confidence: 6.4% Identity: 18% PDB header:oxidoreductase Chain: B: PDB Molecule:allene oxide synthase-lipoxygenase protein; PDBTitle: insights from the x-ray crystal structure of coral 8r-2 lipoxygenase: calcium activation via a c2-like domain and3 a structural basis of product chirality Phyre2 PDB 1c94 chain B Region: 443 - 459 Aligned: 17 Modelled: 17 Confidence: 6.3% Identity: 12% PDB header:gene regulation Chain: B: PDB Molecule:retro-gcn4 leucine zipper; PDBTitle: reversing the sequence of the gcn4 leucine zipper does not2 affect its fold. Phyre2 PDB 2kje chain B Region: 2 - 15 Aligned: 14 Modelled: 14 Confidence: 6.2% Identity: 36% PDB header:transcription Chain: B: PDB Molecule:early e1a 32 kda protein; PDBTitle: nmr structure of cbp taz2 and adenoviral e1a complex Phyre2 1 d1pw4a_ 2 d1pv7a_ 3 c2gfpA_ 4 c3o7pA_ 5 c2xutC_ 6 c3qnqD_ 7 c1m46B_ 8 c2kncB_ 9 c2rdcA_ 10 c1l4aE_ 11 c1by0A_ 12 c3d9sB_ 13 d1sb0a_ 14 d2p0ma1 15 d2b5ua2 16 c2rddB_ 17 c3d3lB_ 18 c2fnqB_ 19 c1c94B_ 20 c2kjeB_ 21 22 23 24 25 26 27 28 29 30 31 32 33

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 d1pw4a_ 100.0 11 Fold:MFS general substrate transporter Superfamily:MFS general substrate transporter Family:Glycerol-3-phosphate transporter 2 d1pv7a_ 100.0 14 Fold:MFS general substrate transporter Superfamily:MFS general substrate transporter Family:LacY-like proton/sugar symporter 3 c2gfpA_ 99.9 11 PDB header:membrane protein Chain: A: PDB Molecule:multidrug resistance protein d; PDBTitle: structure of the multidrug transporter emrd from2 escherichia coli 4 c3o7pA_ 99.9 12 PDB header:transport protein Chain: A: PDB Molecule:l-fucose-proton symporter; PDBTitle: crystal structure of the e.coli fucose:proton symporter, fucp (n162a) 5 c2xutC_ 99.9 11 PDB header:transport protein Chain: C: PDB Molecule:proton/peptide symporter family protein; PDBTitle: crystal structure of a proton dependent oligopeptide (pot)2 family transporter. 6 c3qnqD_ 60.1 14 PDB header:membrane protein, transport protein Chain: D: PDB Molecule:pts system, cellobiose-specific iic component; PDBTitle: crystal structure of the transporter chbc, the iic component from the2 n,n'-diacetylchitobiose-specific phosphotransferase system 7 c1m46B_ 17.4 25 PDB header:cell cycle protein Chain: B: PDB Molecule:iq4 motif from myo2p, a class v myosin; PDBTitle: crystal structure of mlc1p bound to iq4 of myo2p, a class v2 myosin 8 c2kncB_ 15.7 14 PDB header:cell adhesion Chain: B: PDB Molecule:integrin beta-3; PDBTitle: platelet integrin alfaiib-beta3 transmembrane-cytoplasmic2 heterocomplex 9 c2rdcA_ 13.6 15 PDB header:lipid binding protein Chain: A: PDB Molecule:uncharacterized protein; PDBTitle: crystal structure of a putative lipid binding protein (gsu0061) from2 geobacter sulfurreducens pca at 1.80 a resolution 10 c1l4aE_ 13.4 24 PDB header:endocytosis/exocytosis Chain: E: PDB Molecule:synaphin a; PDBTitle: x-ray structure of the neuronal complexin/snare complex2 from the squid loligo pealei 11 c1by0A_ 10.8 13 PDB header:rna binding protein Chain: A: PDB Molecule:protein (hepatitis delta antigen); PDBTitle: n-terminal leucine-repeat region of hepatitis delta antigen 12 c3d9sB_ 9.8 16 PDB header:membrane protein Chain: B: PDB Molecule:aquaporin-5; PDBTitle: human aquaporin 5 (aqp5) - high resolution x-ray structure 13 d1sb0a_ 9.0 27 Fold:Kix domain of CBP (creb binding protein) Superfamily:Kix domain of CBP (creb binding protein) Family:Kix domain of CBP (creb binding protein) 14 d2p0ma1 8.9 24 Fold:Lipoxigenase Superfamily:Lipoxigenase Family:Animal lipoxigenases 15 d2b5ua2 7.9 14 Fold:Cloacin translocation domain Superfamily:Cloacin translocation domain Family:Cloacin translocation domain 16 c2rddB_ 7.6 10 PDB header:membrane protein/transport protein Chain: B: PDB Molecule:upf0092 membrane protein yajc; PDBTitle: x-ray crystal structure of acrb in complex with a novel2 transmembrane helix. 17 c3d3lB_ 6.8 24 PDB header:oxidoreductase Chain: B: PDB Molecule:arachidonate 12-lipoxygenase, 12s-type; PDBTitle: the 2.6 a crystal structure of the lipoxygenase domain of2 human arachidonate 12-lipoxygenase, 12s-type (casp target) 18 c2fnqB_ 6.4 18 PDB header:oxidoreductase Chain: B: PDB Molecule:allene oxide synthase-lipoxygenase protein; PDBTitle: insights from the x-ray crystal structure of coral 8r-2 lipoxygenase: calcium activation via a c2-like domain and3 a structural basis of product chirality 19 c1c94B_ 6.3 12 PDB header:gene regulation Chain: B: PDB Molecule:retro-gcn4 leucine zipper; PDBTitle: reversing the sequence of the gcn4 leucine zipper does not2 affect its fold. 20 c2kjeB_ 6.2 36 PDB header:transcription Chain: B: PDB Molecule:early e1a 32 kda protein; PDBTitle: nmr structure of cbp taz2 and adenoviral e1a complex 21 c2p0mB_ not modelled 6.2 24 PDB header:oxidoreductase Chain: B: PDB Molecule:arachidonate 15-lipoxygenase; PDBTitle: revised structure of rabbit reticulocyte 15s-lipoxygenase 22 c2oarA_ not modelled 6.2 6 PDB header:membrane protein Chain: A: PDB Molecule:large-conductance mechanosensitive channel; PDBTitle: mechanosensitive channel of large conductance (mscl) 23 d1ymga1 not modelled 6.2 8 Fold:Aquaporin-like Superfamily:Aquaporin-like Family:Aquaporin-like 24 c1ymgA_ not modelled 6.2 8 PDB header:membrane protein Chain: A: PDB Molecule:lens fiber major intrinsic protein; PDBTitle: the channel architecture of aquaporin o at 2.2 angstrom resolution 25 c3o8yA_ not modelled 6.1 29 PDB header:oxidoreductase Chain: A: PDB Molecule:arachidonate 5-lipoxygenase; PDBTitle: stable-5-lipoxygenase 26 c3mkuA_ not modelled 5.7 10 PDB header:transport protein Chain: A: PDB Molecule:multi antimicrobial extrusion protein (na(+)/drug PDBTitle: structure of a cation-bound multidrug and toxin compound extrusion2 (mate) transporter 27 d1j4na_ not modelled 5.7 9 Fold:Aquaporin-like Superfamily:Aquaporin-like Family:Aquaporin-like 28 c1a92B_ not modelled 5.6 13 PDB header:leucine zipper Chain: B: PDB Molecule:delta antigen; PDBTitle: oligomerization domain of hepatitis delta antigen 29 c2w8aC_ not modelled 5.4 8 PDB header:membrane protein Chain: C: PDB Molecule:glycine betaine transporter betp; PDBTitle: crystal structure of the sodium-coupled glycine betaine2 symporter betp from corynebacterium glutamicum with bound3 substrate 30 c2hjmB_ not modelled 5.4 8 PDB header:structural genomics, unknown function Chain: B: PDB Molecule:hypothetical protein pf1176; PDBTitle: crystal structure of a singleton protein pf1176 from p. furiosus 31 c2kmfA_ not modelled 5.3 25 PDB header:photosynthesis Chain: A: PDB Molecule:photosystem ii 11 kda protein; PDBTitle: solution structure of psb27 from cyanobacterial photosystem2 ii 32 c3r4hD_ not modelled 5.3 28 PDB header:de novo protein Chain: D: PDB Molecule:coiled coil helix cc-tet-phi22; PDBTitle: crystal structure of the 4-helix coiled coil cc-tet-phi22 33 c3r4hE_ not modelled 5.0 28 PDB header:de novo protein Chain: E: PDB Molecule:coiled coil helix cc-tet-phi22; PDBTitle: crystal structure of the 4-helix coiled coil cc-tet-phi22

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

Transmembrane helix prediction

Transmembrane helices have been predicted in your sequence to adopt the topology shown below

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0