Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	N	T	I	H	L	R	C	L	F	R	M	N	P	L	V	W	C	L	R	A	D	V	A	A	E	L	R	S	L	R	R	Y	Y	H	L	S	N	G	M	E	S	K	S	V	D	T	R	S	I	Y	R	E	L	G	A	T	L	S	Y
Secondary structure
SS confidence
Disorder		?	?	?		?	?	?																	?							?	?				?	?	?							?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		N	M	R	L	G	N	G	M	E	X	E	P	W	L	K	A	A	V	R	K	E	F	V	D	D	N	R	V	K	V	N	N	D	G	N	F	V	N	D	L	S	G	R	R	G	I	Y	Q	A	A	I	K	A	S	F	S	S	T	F	S
Secondary structure
SS confidence
Disorder					?	?	?	?																								?	?	?	?	?	?	?	?	?		?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.
Sequence		G	H	L	G	V	G	Y	S	H	G	A	G	V	E	S	P	W	N	A	V	A	G	V	N	W	S	F
Secondary structure
SS confidence
Disorder								?	?	?	?	?	?	?	?	?												?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 3slj chain A Region: 3 - 147 Aligned: 144 Modelled: 144 Confidence: 100.0% Identity: 8% PDB header:protein transport Chain: A: PDB Molecule:serine protease espp; PDBTitle: pre-cleavage structure of the autotransporter espp - n1023a mutant Phyre2 PDB 3qq2 chain C Region: 3 - 147 Aligned: 131 Modelled: 131 Confidence: 100.0% Identity: 20% PDB header:membrane protein/protein transport Chain: C: PDB Molecule:brka autotransporter; PDBTitle: crystal structure of the beta domain of the bordetella autotransporter2 brka Phyre2 PDB 2qom chain B Region: 3 - 147 Aligned: 143 Modelled: 143 Confidence: 99.9% Identity: 8% PDB header:hydrolase Chain: B: PDB Molecule:serine protease espp; PDBTitle: the crystal structure of the e.coli espp autotransporter beta-domain. Phyre2 PDB 1uyn chain X Region: 3 - 147 Aligned: 128 Modelled: 128 Confidence: 99.9% Identity: 13% Fold: Transmembrane beta-barrels Superfamily: Autotransporter Family: Autotransporter Phyre2 PDB 3aeh chain B Region: 3 - 147 Aligned: 118 Modelled: 118 Confidence: 99.9% Identity: 14% PDB header:hydrolase Chain: B: PDB Molecule:hemoglobin-binding protease hbp autotransporter; PDBTitle: integral membrane domain of autotransporter hbp Phyre2 PDB 3kvn chain A Region: 3 - 147 Aligned: 140 Modelled: 140 Confidence: 99.9% Identity: 13% PDB header:hydrolase Chain: A: PDB Molecule:esterase esta; PDBTitle: crystal structure of the full-length autotransporter esta from2 pseudomonas aeruginosa Phyre2 PDB 3qra chain A Region: 1 - 147 Aligned: 126 Modelled: 126 Confidence: 98.8% Identity: 15% PDB header:cell invasion Chain: A: PDB Molecule:attachment invasion locus protein; PDBTitle: the crystal structure of ail, the attachment invasion locus protein of2 yersinia pestis Phyre2 PDB 1qj8 chain A Region: 4 - 147 Aligned: 124 Modelled: 124 Confidence: 98.8% Identity: 15% Fold: Transmembrane beta-barrels Superfamily: OMPA-like Family: Outer membrane protein Phyre2 PDB 2x27 chain X Region: 3 - 147 Aligned: 139 Modelled: 139 Confidence: 98.6% Identity: 10% PDB header:membrane protein Chain: X: PDB Molecule:outer membrane protein oprg; PDBTitle: crystal structure of the outer membrane protein oprg from2 pseudomonas aeruginosa Phyre2 PDB 1qjp chain A Region: 3 - 147 Aligned: 114 Modelled: 114 Confidence: 98.6% Identity: 12% Fold: Transmembrane beta-barrels Superfamily: OMPA-like Family: Outer membrane protein Phyre2 PDB 1g90 chain A Region: 3 - 147 Aligned: 128 Modelled: 128 Confidence: 98.5% Identity: 15% Fold: Transmembrane beta-barrels Superfamily: OMPA-like Family: Outer membrane protein Phyre2 PDB 2f1t chain B Region: 3 - 147 Aligned: 136 Modelled: 136 Confidence: 98.5% Identity: 13% PDB header:membrane protein Chain: B: PDB Molecule:outer membrane protein w; PDBTitle: outer membrane protein ompw Phyre2 PDB 3nb3 chain C Region: 3 - 147 Aligned: 114 Modelled: 114 Confidence: 98.5% Identity: 14% PDB header:virus Chain: C: PDB Molecule:outer membrane protein a; PDBTitle: the host outer membrane proteins ompa and ompc are packed at specific2 sites in the shigella phage sf6 virion as structural components Phyre2 PDB 1p4t chain A Region: 15 - 147 Aligned: 126 Modelled: 126 Confidence: 98.5% Identity: 17% Fold: Transmembrane beta-barrels Superfamily: OMPA-like Family: Outer membrane protein Phyre2 PDB 2k0l chain A Region: 10 - 147 Aligned: 132 Modelled: 132 Confidence: 98.5% Identity: 6% PDB header:membrane protein Chain: A: PDB Molecule:outer membrane protein a; PDBTitle: nmr structure of the transmembrane domain of the outer2 membrane protein a from klebsiella pneumoniae in dhpc3 micelles. Phyre2 PDB 2jmm chain A Region: 3 - 147 Aligned: 124 Modelled: 124 Confidence: 98.3% Identity: 11% PDB header:membrane protein Chain: A: PDB Molecule:outer membrane protein a; PDBTitle: nmr solution structure of a minimal transmembrane beta-2 barrel platform protein Phyre2 PDB 2lhf chain A Region: 21 - 147 Aligned: 121 Modelled: 121 Confidence: 98.1% Identity: 19% PDB header:membrane protein Chain: A: PDB Molecule:outer membrane protein h1; PDBTitle: solution structure of outer membrane protein h (oprh) from p.2 aeruginosa in dhpc micelles Phyre2 PDB 2iwv chain D Region: 4 - 147 Aligned: 124 Modelled: 124 Confidence: 97.8% Identity: 17% PDB header:ion channel Chain: D: PDB Molecule:outer membrane protein g; PDBTitle: structure of the monomeric outer membrane porin ompg in the2 open and closed conformation Phyre2 PDB 2wjq chain A Region: 4 - 146 Aligned: 138 Modelled: 138 Confidence: 97.1% Identity: 8% PDB header:transport protein Chain: A: PDB Molecule:probable n-acetylneuraminic acid outer membrane channel PDBTitle: nanc porin structure in hexagonal crystal form. Phyre2 PDB 2fgq chain X domain 1 Region: 21 - 147 Aligned: 127 Modelled: 127 Confidence: 97.1% Identity: 9% Fold: Transmembrane beta-barrels Superfamily: Porins Family: Porin Phyre2 1 c3sljA_ 2 c3qq2C_ 3 c2qomB_ 4 d1uynx_ 5 c3aehB_ 6 c3kvnA_ 7 c3qraA_ 8 d1qj8a_ 9 c2x27X_ 10 d1qjpa_ 11 d1g90a_ 12 c2f1tB_ 13 c3nb3C_ 14 d1p4ta_ 15 c2k0lA_ 16 c2jmmA_ 17 c2lhfA_ 18 c2iwvD_ 19 c2wjqA_ 20 d2fgqx1 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 c3sljA_ 100.0 8 PDB header:protein transport Chain: A: PDB Molecule:serine protease espp; PDBTitle: pre-cleavage structure of the autotransporter espp - n1023a mutant 2 c3qq2C_ 100.0 20 PDB header:membrane protein/protein transport Chain: C: PDB Molecule:brka autotransporter; PDBTitle: crystal structure of the beta domain of the bordetella autotransporter2 brka 3 c2qomB_ 99.9 8 PDB header:hydrolase Chain: B: PDB Molecule:serine protease espp; PDBTitle: the crystal structure of the e.coli espp autotransporter beta-domain. 4 d1uynx_ 99.9 13 Fold:Transmembrane beta-barrels Superfamily:Autotransporter Family:Autotransporter 5 c3aehB_ 99.9 14 PDB header:hydrolase Chain: B: PDB Molecule:hemoglobin-binding protease hbp autotransporter; PDBTitle: integral membrane domain of autotransporter hbp 6 c3kvnA_ 99.9 13 PDB header:hydrolase Chain: A: PDB Molecule:esterase esta; PDBTitle: crystal structure of the full-length autotransporter esta from2 pseudomonas aeruginosa 7 c3qraA_ 98.8 15 PDB header:cell invasion Chain: A: PDB Molecule:attachment invasion locus protein; PDBTitle: the crystal structure of ail, the attachment invasion locus protein of2 yersinia pestis 8 d1qj8a_ 98.8 15 Fold:Transmembrane beta-barrels Superfamily:OMPA-like Family:Outer membrane protein 9 c2x27X_ 98.6 10 PDB header:membrane protein Chain: X: PDB Molecule:outer membrane protein oprg; PDBTitle: crystal structure of the outer membrane protein oprg from2 pseudomonas aeruginosa 10 d1qjpa_ 98.6 12 Fold:Transmembrane beta-barrels Superfamily:OMPA-like Family:Outer membrane protein 11 d1g90a_ 98.5 15 Fold:Transmembrane beta-barrels Superfamily:OMPA-like Family:Outer membrane protein 12 c2f1tB_ 98.5 13 PDB header:membrane protein Chain: B: PDB Molecule:outer membrane protein w; PDBTitle: outer membrane protein ompw 13 c3nb3C_ 98.5 14 PDB header:virus Chain: C: PDB Molecule:outer membrane protein a; PDBTitle: the host outer membrane proteins ompa and ompc are packed at specific2 sites in the shigella phage sf6 virion as structural components 14 d1p4ta_ 98.5 17 Fold:Transmembrane beta-barrels Superfamily:OMPA-like Family:Outer membrane protein 15 c2k0lA_ 98.5 6 PDB header:membrane protein Chain: A: PDB Molecule:outer membrane protein a; PDBTitle: nmr structure of the transmembrane domain of the outer2 membrane protein a from klebsiella pneumoniae in dhpc3 micelles. 16 c2jmmA_ 98.3 11 PDB header:membrane protein Chain: A: PDB Molecule:outer membrane protein a; PDBTitle: nmr solution structure of a minimal transmembrane beta-2 barrel platform protein 17 c2lhfA_ 98.1 19 PDB header:membrane protein Chain: A: PDB Molecule:outer membrane protein h1; PDBTitle: solution structure of outer membrane protein h (oprh) from p.2 aeruginosa in dhpc micelles 18 c2iwvD_ 97.8 17 PDB header:ion channel Chain: D: PDB Molecule:outer membrane protein g; PDBTitle: structure of the monomeric outer membrane porin ompg in the2 open and closed conformation 19 c2wjqA_ 97.1 8 PDB header:transport protein Chain: A: PDB Molecule:probable n-acetylneuraminic acid outer membrane channel PDBTitle: nanc porin structure in hexagonal crystal form. 20 d2fgqx1 97.1 9 Fold:Transmembrane beta-barrels Superfamily:Porins Family:Porin 21 d1i78a_ not modelled 96.8 12 Fold:Transmembrane beta-barrels Superfamily:OMPT-like Family:Outer membrane protease OMPT 22 c2x4mD_ not modelled 96.7 19 PDB header:hydrolase Chain: D: PDB Molecule:coagulase/fibrinolysin; PDBTitle: yersinia pestis plasminogen activator pla 23 d1t16a_ not modelled 96.5 13 Fold:Transmembrane beta-barrels Superfamily:Porins Family:Outer membrane protein transport protein 24 d1osma_ not modelled 96.4 11 Fold:Transmembrane beta-barrels Superfamily:Porins Family:Porin 25 d3prna_ not modelled 96.3 24 Fold:Transmembrane beta-barrels Superfamily:Porins Family:Porin 26 d2zfga1 not modelled 96.2 12 Fold:Transmembrane beta-barrels Superfamily:Porins Family:Porin 27 d2pora_ not modelled 95.9 13 Fold:Transmembrane beta-barrels Superfamily:Porins Family:Porin 28 c3a2rX_ not modelled 95.4 13 PDB header:membrane protein Chain: X: PDB Molecule:outer membrane protein ii; PDBTitle: crystal structure of outer membrane protein porb from neisseria2 meningitidis 29 c3brzA_ not modelled 95.4 12 PDB header:transport protein Chain: A: PDB Molecule:todx; PDBTitle: crystal structure of the pseudomonas putida toluene2 transporter todx 30 d1phoa_ not modelled 95.3 9 Fold:Transmembrane beta-barrels Superfamily:Porins Family:Porin 31 c3dwoX_ not modelled 94.9 8 PDB header:membrane protein Chain: X: PDB Molecule:probable outer membrane protein; PDBTitle: crystal structure of a pseudomonas aeruginosa fadl homologue 32 c3bryB_ not modelled 94.6 8 PDB header:transport protein Chain: B: PDB Molecule:tbux; PDBTitle: crystal structure of the ralstonia pickettii toluene2 transporter tbux 33 c3nsgA_ not modelled 87.0 11 PDB header:membrane protein Chain: A: PDB Molecule:outer membrane protein f; PDBTitle: crystal structure of ompf, an outer membrane protein from salmonella2 typhi 34 c2hdiA_ not modelled 86.9 10 PDB header:protein transport,antimicrobial protein Chain: A: PDB Molecule:colicin i receptor; PDBTitle: crystal structure of the colicin i receptor cir from e.coli in complex2 with receptor binding domain of colicin ia. 35 c2iahA_ not modelled 86.8 12 PDB header:membrane protein Chain: A: PDB Molecule:ferripyoverdine receptor; PDBTitle: crystal structure of the ferripyoverdine receptor of the outer2 membrane of pseudomonas aeruginosa bound to ferripyoverdine. 36 c2o4vA_ not modelled 75.5 8 PDB header:membrane protein Chain: A: PDB Molecule:porin p; PDBTitle: an arginine ladder in oprp mediates phosphate specific transfer across2 the outer membrane 37 c1xkhC_ not modelled 64.1 12 PDB header:membrane protein Chain: C: PDB Molecule:ferripyoverdine receptor; PDBTitle: pyoverdine outer membrane receptor fpva from pseudomonas aeruginosa2 pao1 bound to pyoverdine 38 c1xkwA_ not modelled 45.2 8 PDB header:membrane protein Chain: A: PDB Molecule:fe(iii)-pyochelin receptor; PDBTitle: pyochelin outer membrane receptor fpta from pseudomonas2 aeruginosa 39 d1by5a_ not modelled 39.3 11 Fold:Transmembrane beta-barrels Superfamily:Porins Family:Ligand-gated protein channel 40 c2grxB_ not modelled 38.0 11 PDB header:metal transport Chain: B: PDB Molecule:ferrichrome-iron receptor; PDBTitle: crystal structure of tonb in complex with fhua, e. coli2 outer membrane receptor for ferrichrome 41 c3cslB_ not modelled 37.8 11 PDB header:membrane protein/heme binding protein Chain: B: PDB Molecule:hasr protein; PDBTitle: structure of the serratia marcescens hemophore receptor hasr in2 complex with its hemophore hasa and heme 42 c2gr7C_ not modelled 32.5 16 PDB header:membrane protein Chain: C: PDB Molecule:adhesin; PDBTitle: hia 992-1098 43 d2gr7a1 not modelled 32.5 16 Fold:Pili subunits Superfamily:Pili subunits Family:YadA C-terminal domain-like 44 d2gufa1 not modelled 23.7 8 Fold:Transmembrane beta-barrels Superfamily:Porins Family:Ligand-gated protein channel 45 c3qlbA_ not modelled 20.5 10 PDB header:metal transport Chain: A: PDB Molecule:enantio-pyochelin receptor; PDBTitle: enantiopyochelin outer membrane tonb-dependent transporter from2 pseudomonas fluorescens bound to the ferri-enantiopyochelin 46 c3emoA_ not modelled 19.5 11 PDB header:membrane protein/cell adhesion Chain: A: PDB Molecule:hia (adhesin); PDBTitle: crystal structure of transmembrane hia 973-1098 47 d2gr8a1 not modelled 19.1 16 Fold:Pili subunits Superfamily:Pili subunits Family:YadA C-terminal domain-like 48 d2vdfa1 not modelled 18.1 16 Fold:Transmembrane beta-barrels Superfamily:OMPT-like Family:Outer membrane adhesin/invasin OpcA 49 c1po3A_ not modelled 12.8 10 PDB header:membrane protein Chain: A: PDB Molecule:iron(iii) dicitrate transport protein feca PDBTitle: crystal structure of ferric citrate transporter feca in2 complex with ferric citrate 50 c2k4tA_ not modelled 12.3 9 PDB header:membrane protein,apoptosis Chain: A: PDB Molecule:voltage-dependent anion-selective channel PDBTitle: solution structure of human vdac-1 in ldao micelles 51 d1nkga3 not modelled 10.7 20 Fold:Supersandwich Superfamily:Galactose mutarotase-like Family:Rhamnogalacturonase B, RhgB, N-terminal domain 52 c1yueA_ not modelled 9.3 11 PDB header:viral protein Chain: A: PDB Molecule:head vertex protein gp24; PDBTitle: bacteriophage t4 capsid vertex protein gp24 53 d1kmoa_ not modelled 9.1 10 Fold:Transmembrane beta-barrels Superfamily:Porins Family:Ligand-gated protein channel 54 c1xcqA_ not modelled 8.4 13 PDB header:immune system Chain: A: PDB Molecule:monoclonal antibody 19d9d6 light chain; PDBTitle: complex hcv core-fab 19d9d6-protein l mutant (d55a,l57h,y64w) in space2 group p21 55 d1x99a_ not modelled 7.4 22 Fold:Cytolysin/lectin Superfamily:Cytolysin/lectin Family:Fungal fruit body lectin 56 c3efmA_ not modelled 6.8 19 PDB header:membrane protein Chain: A: PDB Molecule:ferric alcaligin siderophore receptor; PDBTitle: structure of the alcaligin outer membrane recepteur faua from2 bordetella pertussis 57 d1txka2 not modelled 6.8 10 Fold:Supersandwich Superfamily:Galactose mutarotase-like Family:MdoG-like

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0