Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	T	S	L	Q	L	S	I	V	H	R	L	P	Q	N	Y	R	W	S	A	G	F	A	G	S	K	V	E	P	I	P	Q	N	G	P	C	G	D	N	S	L	V	A	L	K	L	L	S	P	D	G	D	N	A	W	S	V	M	Y	K
Secondary structure
SS confidence
Disorder		?	?	?	?																												?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.
Sequence		L	S	Q	A	L	S	D	I	E	V	P	C	S	V	L	E	C	E	G	E	P	C	L	F	V	N	R	Q	D	E	F	A	A	T	C	R	L	K	N	F	G	V	A	I	A	E	P	F	S	N	Y	N	P	F
Secondary structure
SS confidence
Disorder										?																																							?	?	?	?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 2cx1 chain A domain 2 Region: 71 - 102 Aligned: 32 Modelled: 32 Confidence: 85.5% Identity: 31% Fold: Cystatin-like Superfamily: Pre-PUA domain Family: Hypothetical protein APE0525, N-terminal domain Phyre2 PDB 1zhv chain A Region: 2 - 104 Aligned: 87 Modelled: 88 Confidence: 69.9% Identity: 22% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:hypothetical protein atu0741; PDBTitle: x-ray crystal structure protein atu0741 from agobacterium tumefaciens.2 northeast structural genomics consortium target atr8. Phyre2 PDB 1zhv chain A domain 2 Region: 43 - 104 Aligned: 62 Modelled: 62 Confidence: 57.5% Identity: 18% Fold: Ferredoxin-like Superfamily: ACT-like Family: Atu0741-like Phyre2 PDB 1ev7 chain A Region: 76 - 90 Aligned: 15 Modelled: 15 Confidence: 33.9% Identity: 33% Fold: Restriction endonuclease-like Superfamily: Restriction endonuclease-like Family: Restriction endonuclease NaeI Phyre2 PDB 1w96 chain A domain 1 Region: 14 - 71 Aligned: 56 Modelled: 58 Confidence: 33.2% Identity: 7% Fold: Barrel-sandwich hybrid Superfamily: Rudiment single hybrid motif Family: BC C-terminal domain-like Phyre2 PDB 1e32 chain A domain 3 Region: 71 - 90 Aligned: 17 Modelled: 20 Confidence: 29.7% Identity: 59% Fold: Cdc48 domain 2-like Superfamily: Cdc48 domain 2-like Family: Cdc48 domain 2-like Phyre2 PDB 3zqu chain A Region: 94 - 112 Aligned: 19 Modelled: 19 Confidence: 25.8% Identity: 21% PDB header:lyase Chain: A: PDB Molecule:probable aromatic acid decarboxylase; PDBTitle: structure of a probable aromatic acid decarboxylase Phyre2 PDB 3qoc chain D Region: 13 - 93 Aligned: 37 Modelled: 37 Confidence: 24.2% Identity: 22% PDB header:hydrolase Chain: D: PDB Molecule:putative metallopeptidase; PDBTitle: crystal structure of n-terminal domain (creatinase/prolidase like2 domain) of putative metallopeptidase from corynebacterium diphtheriae Phyre2 PDB 1n1t chain A domain 2 Region: 29 - 89 Aligned: 58 Modelled: 61 Confidence: 24.0% Identity: 19% Fold: 6-bladed beta-propeller Superfamily: Sialidases Family: Sialidases (neuraminidases) Phyre2 PDB 2j9g chain A domain 1 Region: 14 - 71 Aligned: 56 Modelled: 58 Confidence: 23.9% Identity: 18% Fold: Barrel-sandwich hybrid Superfamily: Rudiment single hybrid motif Family: BC C-terminal domain-like Phyre2 PDB 2qhs chain A Region: 50 - 110 Aligned: 61 Modelled: 61 Confidence: 23.3% Identity: 11% PDB header:transferase Chain: A: PDB Molecule:lipoyltransferase; PDBTitle: structural basis of octanoic acid recognition by lipoate-protein2 ligase b Phyre2 PDB 2qhv chain A Region: 50 - 110 Aligned: 61 Modelled: 61 Confidence: 23.3% Identity: 11% PDB header:transferase Chain: A: PDB Molecule:lipoyltransferase; PDBTitle: structural basis of octanoic acid recognition by lipoate-protein2 ligase b Phyre2 PDB 1p9o chain A Region: 36 - 90 Aligned: 48 Modelled: 55 Confidence: 22.4% Identity: 15% Fold: Ribokinase-like Superfamily: CoaB-like Family: CoaB-like Phyre2 PDB 2ejb chain A Region: 96 - 112 Aligned: 17 Modelled: 17 Confidence: 21.2% Identity: 6% PDB header:lyase Chain: A: PDB Molecule:probable aromatic acid decarboxylase; PDBTitle: crystal structure of phenylacrylic acid decarboxylase from2 aquifex aeolicus Phyre2 PDB 1n1v chain A Region: 29 - 89 Aligned: 58 Modelled: 61 Confidence: 19.6% Identity: 19% PDB header:hydrolase Chain: A: PDB Molecule:sialidase; PDBTitle: trypanosoma rangeli sialidase in complex with dana Phyre2 PDB 1ulz chain A domain 1 Region: 14 - 71 Aligned: 56 Modelled: 58 Confidence: 19.6% Identity: 20% Fold: Barrel-sandwich hybrid Superfamily: Rudiment single hybrid motif Family: BC C-terminal domain-like Phyre2 PDB 1zvp chain A domain 2 Region: 41 - 100 Aligned: 56 Modelled: 60 Confidence: 19.5% Identity: 20% Fold: Ferredoxin-like Superfamily: ACT-like Family: VC0802-like Phyre2 PDB 1sbz chain A Region: 96 - 112 Aligned: 17 Modelled: 17 Confidence: 18.4% Identity: 29% Fold: Homo-oligomeric flavin-containing Cys decarboxylases, HFCD Superfamily: Homo-oligomeric flavin-containing Cys decarboxylases, HFCD Family: Homo-oligomeric flavin-containing Cys decarboxylases, HFCD Phyre2 PDB 1w96 chain C domain 1 Region: 14 - 71 Aligned: 56 Modelled: 58 Confidence: 18.3% Identity: 11% Fold: Barrel-sandwich hybrid Superfamily: Rudiment single hybrid motif Family: BC C-terminal domain-like Phyre2 PDB 3a5z chain F Region: 74 - 88 Aligned: 15 Modelled: 15 Confidence: 18.0% Identity: 20% PDB header:ligase Chain: F: PDB Molecule:elongation factor p; PDBTitle: crystal structure of escherichia coli genx in complex with elongation2 factor p Phyre2 1 d2cx1a2 2 c1zhvA_ 3 d1zhva2 4 d1ev7a_ 5 d1w96a1 6 d1e32a3 7 c3zquA_ 8 c3qocD_ 9 d1n1ta2 10 d2j9ga1 11 c2qhsA_ 12 c2qhvA_ 13 d1p9oa_ 14 c2ejbA_ 15 c1n1vA_ 16 d1ulza1 17 d1zvpa2 18 d1sbza_ 19 d1w96c1 20 c3a5zF_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 d2cx1a2 85.5 31 Fold:Cystatin-like Superfamily:Pre-PUA domain Family:Hypothetical protein APE0525, N-terminal domain 2 c1zhvA_ 69.9 22 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:hypothetical protein atu0741; PDBTitle: x-ray crystal structure protein atu0741 from agobacterium tumefaciens.2 northeast structural genomics consortium target atr8. 3 d1zhva2 57.5 18 Fold:Ferredoxin-like Superfamily:ACT-like Family:Atu0741-like 4 d1ev7a_ 33.9 33 Fold:Restriction endonuclease-like Superfamily:Restriction endonuclease-like Family:Restriction endonuclease NaeI 5 d1w96a1 33.2 7 Fold:Barrel-sandwich hybrid Superfamily:Rudiment single hybrid motif Family:BC C-terminal domain-like 6 d1e32a3 29.7 59 Fold:Cdc48 domain 2-like Superfamily:Cdc48 domain 2-like Family:Cdc48 domain 2-like 7 c3zquA_ 25.8 21 PDB header:lyase Chain: A: PDB Molecule:probable aromatic acid decarboxylase; PDBTitle: structure of a probable aromatic acid decarboxylase 8 c3qocD_ 24.2 22 PDB header:hydrolase Chain: D: PDB Molecule:putative metallopeptidase; PDBTitle: crystal structure of n-terminal domain (creatinase/prolidase like2 domain) of putative metallopeptidase from corynebacterium diphtheriae 9 d1n1ta2 24.0 19 Fold:6-bladed beta-propeller Superfamily:Sialidases Family:Sialidases (neuraminidases) 10 d2j9ga1 23.9 18 Fold:Barrel-sandwich hybrid Superfamily:Rudiment single hybrid motif Family:BC C-terminal domain-like 11 c2qhsA_ 23.3 11 PDB header:transferase Chain: A: PDB Molecule:lipoyltransferase; PDBTitle: structural basis of octanoic acid recognition by lipoate-protein2 ligase b 12 c2qhvA_ 23.3 11 PDB header:transferase Chain: A: PDB Molecule:lipoyltransferase; PDBTitle: structural basis of octanoic acid recognition by lipoate-protein2 ligase b 13 d1p9oa_ 22.4 15 Fold:Ribokinase-like Superfamily:CoaB-like Family:CoaB-like 14 c2ejbA_ 21.2 6 PDB header:lyase Chain: A: PDB Molecule:probable aromatic acid decarboxylase; PDBTitle: crystal structure of phenylacrylic acid decarboxylase from2 aquifex aeolicus 15 c1n1vA_ 19.6 19 PDB header:hydrolase Chain: A: PDB Molecule:sialidase; PDBTitle: trypanosoma rangeli sialidase in complex with dana 16 d1ulza1 19.6 20 Fold:Barrel-sandwich hybrid Superfamily:Rudiment single hybrid motif Family:BC C-terminal domain-like 17 d1zvpa2 19.5 20 Fold:Ferredoxin-like Superfamily:ACT-like Family:VC0802-like 18 d1sbza_ 18.4 29 Fold:Homo-oligomeric flavin-containing Cys decarboxylases, HFCD Superfamily:Homo-oligomeric flavin-containing Cys decarboxylases, HFCD Family:Homo-oligomeric flavin-containing Cys decarboxylases, HFCD 19 d1w96c1 18.3 11 Fold:Barrel-sandwich hybrid Superfamily:Rudiment single hybrid motif Family:BC C-terminal domain-like 20 c3a5zF_ 18.0 20 PDB header:ligase Chain: F: PDB Molecule:elongation factor p; PDBTitle: crystal structure of escherichia coli genx in complex with elongation2 factor p 21 c2k7mA_ not modelled 16.9 71 PDB header:membrane protein Chain: A: PDB Molecule:gap junction alpha-5 protein; PDBTitle: structure of the connexin40 carboxyl terminal domain 22 c2g7rA_ not modelled 16.5 25 PDB header:hydrolase Chain: A: PDB Molecule:mucosa-associated lymphoid tissue lymphoma translocation PDBTitle: x-ray structure of the death domain of the human mucosa associated2 lymphoid tissue lymphoma translocation protein 1 23 c2eifA_ not modelled 16.3 25 PDB header:gene regulation Chain: A: PDB Molecule:protein (eukaryotic translation initiation factor 5a); PDBTitle: eukaryotic translation initiation factor 5a from methanococcus2 jannaschii 24 c3hksB_ not modelled 14.3 19 PDB header:translation, rna binding protein Chain: B: PDB Molecule:eukaryotic translation initiation factor 5a-2; PDBTitle: crystal structure of eukaryotic translation initiation2 factor eif-5a2 from arabidopsis thaliana 25 d2ah2a2 not modelled 14.2 13 Fold:6-bladed beta-propeller Superfamily:Sialidases Family:Sialidases (neuraminidases) 26 c3oooA_ not modelled 13.4 18 PDB header:hydrolase Chain: A: PDB Molecule:proline dipeptidase; PDBTitle: the structure of a proline dipeptidase from streptococcus agalactiae2 2603v 27 d1ueba1 not modelled 12.3 20 Fold:SH3-like barrel Superfamily:Translation proteins SH3-like domain Family:eIF5a N-terminal domain-like 28 c3b64A_ not modelled 11.5 10 PDB header:cytokine Chain: A: PDB Molecule:macrophage migration inhibitory factor-like PDBTitle: macrophage migration inhibitory factor (mif) from2 /leishmania major 29 d1fima_ not modelled 11.5 16 Fold:Tautomerase/MIF Superfamily:Tautomerase/MIF Family:MIF-related 30 d2g2xa1 not modelled 11.2 18 Fold:PUA domain-like Superfamily:PUA domain-like Family:Atu2648/PH1033-like 31 d1vk6a3 not modelled 11.1 35 Fold:Nudix Superfamily:Nudix Family:NADH pyrophosphatase 32 c3kdpD_ not modelled 11.0 47 PDB header:hydrolase Chain: D: PDB Molecule:sodium/potassium-transporting atpase subunit beta-1; PDBTitle: crystal structure of the sodium-potassium pump 33 c2h31A_ not modelled 10.3 21 PDB header:ligase, lyase Chain: A: PDB Molecule:multifunctional protein ade2; PDBTitle: crystal structure of human paics, a bifunctional carboxylase and2 synthetase in purine biosynthesis 34 d1pv8a_ not modelled 9.5 19 Fold:TIM beta/alpha-barrel Superfamily:Aldolase Family:5-aminolaevulinate dehydratase, ALAD (porphobilinogen synthase) 35 c3fwtA_ not modelled 8.4 11 PDB header:cytokine Chain: A: PDB Molecule:macrophage migration inhibitory factor-like PDBTitle: crystal structure of leishmania major mif2 36 c2zxeB_ not modelled 8.0 44 PDB header:hydrolase/transport protein Chain: B: PDB Molecule:na+,k+-atpase beta subunit; PDBTitle: crystal structure of the sodium - potassium pump in the e2.2k+.pi2 state 37 c2wk1A_ not modelled 7.8 23 PDB header:transferase Chain: A: PDB Molecule:novp; PDBTitle: structure of the o-methyltransferase novp 38 c3krnB_ not modelled 7.8 15 PDB header:hydrolase Chain: B: PDB Molecule:protein c14a4.5, confirmed by transcript evidence; PDBTitle: crystal structure of c. elegans cell-death-related nuclease 5(crn-5) 39 d2gbsa1 not modelled 7.2 22 Fold:PUA domain-like Superfamily:PUA domain-like Family:Atu2648/PH1033-like 40 d2gdta1 not modelled 7.1 33 Fold:SARS Nsp1-like Superfamily:SARS Nsp1-like Family:SARS Nsp1-like 41 c3i7mA_ not modelled 7.1 18 PDB header:hydrolase Chain: A: PDB Molecule:xaa-pro dipeptidase; PDBTitle: n-terminal domain of xaa-pro dipeptidase from lactobacillus brevis. 42 d1gd0a_ not modelled 7.1 18 Fold:Tautomerase/MIF Superfamily:Tautomerase/MIF Family:MIF-related 43 c3er0A_ not modelled 6.9 19 PDB header:translation Chain: A: PDB Molecule:eukaryotic translation initiation factor 5a-2; PDBTitle: crystal structure of the full length eif5a from2 saccharomyces cerevisiae 44 c2gk4A_ not modelled 6.7 15 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:conserved hypothetical protein; PDBTitle: the crystal structure of the dna/pantothenate metabolism flavoprotein2 from streptococcus pneumoniae 45 d1riea_ not modelled 6.7 15 Fold:ISP domain Superfamily:ISP domain Family:Rieske iron-sulfur protein (ISP) 46 d2prva1 not modelled 6.5 40 Fold:SMI1/KNR4-like Superfamily:SMI1/KNR4-like Family:SMI1/KNR4-like 47 d1u7za_ not modelled 6.4 11 Fold:Ribokinase-like Superfamily:CoaB-like Family:CoaB-like 48 d1laue_ not modelled 6.4 11 Fold:Uracil-DNA glycosylase-like Superfamily:Uracil-DNA glycosylase-like Family:Uracil-DNA glycosylase 49 c3o5vA_ not modelled 6.3 23 PDB header:hydrolase Chain: A: PDB Molecule:x-pro dipeptidase; PDBTitle: the crystal structure of the creatinase/prolidase n-terminal domain of2 an x-pro dipeptidase from streptococcus pyogenes to 1.85a 50 c3c9pA_ not modelled 6.3 20 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:uncharacterized protein sp1917; PDBTitle: crystal structure of uncharacterized protein sp1917 51 c2f06B_ not modelled 6.1 15 PDB header:structural genomics, unknown function Chain: B: PDB Molecule:conserved hypothetical protein; PDBTitle: crystal structure of protein bt0572 from bacteroides thetaiotaomicron 52 d2evea1 not modelled 6.0 19 Fold:PUA domain-like Superfamily:PUA domain-like Family:Atu2648/PH1033-like 53 c3gacD_ not modelled 5.7 20 PDB header:cytokine Chain: D: PDB Molecule:macrophage migration inhibitory factor-like PDBTitle: structure of mif with hpp 54 d1hfoa_ not modelled 5.7 15 Fold:Tautomerase/MIF Superfamily:Tautomerase/MIF Family:MIF-related 55 d2gdga1 not modelled 5.7 16 Fold:Tautomerase/MIF Superfamily:Tautomerase/MIF Family:MIF-related 56 c3ic8D_ not modelled 5.6 24 PDB header:structural genomics, unknown function Chain: D: PDB Molecule:uncharacterized gst-like proteinprotein; PDBTitle: the crystal structure of a gst-like protein from pseudomonas syringae2 to 2.4a 57 c3treA_ not modelled 5.6 14 PDB header:translation Chain: A: PDB Molecule:elongation factor p; PDBTitle: structure of a translation elongation factor p (efp) from coxiella2 burnetii 58 d1u11a_ not modelled 5.4 20 Fold:Flavodoxin-like Superfamily:N5-CAIR mutase (phosphoribosylaminoimidazole carboxylase, PurE) Family:N5-CAIR mutase (phosphoribosylaminoimidazole carboxylase, PurE) 59 c2fw9A_ not modelled 5.4 20 PDB header:lyase Chain: A: PDB Molecule:n5-carboxyaminoimidazole ribonucleotide mutase; PDBTitle: structure of pure (n5-carboxyaminoimidazole ribonucleotide mutase)2 h59f from the acidophilic bacterium acetobacter aceti, at ph 8 60 c4a1eF_ not modelled 5.4 24 PDB header:ribosome Chain: F: PDB Molecule:rpl7a; PDBTitle: t.thermophila 60s ribosomal subunit in complex with2 initiation factor 6. this file contains 5s rrna, 5.8s rrna3 and proteins of molecule 1 61 c2kllA_ not modelled 5.3 27 PDB header:cytokine Chain: A: PDB Molecule:interleukin-33; PDBTitle: solution structure of human interleukin-33 62 d1xgsa1 not modelled 5.3 33 Fold:DNA/RNA-binding 3-helical bundle Superfamily:"Winged helix" DNA-binding domain Family:Methionine aminopeptidase, insert domain 63 d1zcea1 not modelled 5.2 18 Fold:PUA domain-like Superfamily:PUA domain-like Family:Atu2648/PH1033-like 64 d1okba_ not modelled 5.2 17 Fold:Uracil-DNA glycosylase-like Superfamily:Uracil-DNA glycosylase-like Family:Uracil-DNA glycosylase 65 c3lodA_ not modelled 5.1 9 PDB header:transferase Chain: A: PDB Molecule:putative acyl-coa n-acyltransferase; PDBTitle: the crystal structure of the putative acyl-coa n-acyltransferase from2 klebsiella pneumoniae subsp.pneumoniae mgh 78578 66 d2icga1 not modelled 5.1 20 Fold:SMI1/KNR4-like Superfamily:SMI1/KNR4-like Family:SMI1/KNR4-like

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

---

Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0