Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	A	T	G	K	S	C	S	R	W	F	A	P	L	A	A	L	L	M	V	V	S	L	S	G	C	F	D	K	E	G	D	Q	R	K	A	F	I	D	F	L	Q	N	T	V	M	R	S	G	E	R	L	P	T	L	T	A	D	Q	K
Secondary structure
SS confidence
Disorder		?	?	?	?																						?	?	?	?	?														?	?	?	?	?	?	?				?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		K	Q	F	G	P	F	V	S	D	Y	A	I	L	Y	G	Y	S	Q	Q	V	N	Q	A	M	D	S	G	L	R	P	V	V	D	S	V	N	A	I	R	V	P	Q	D	Y	V	T	Q	S	G	P	L	R	E	M	N	G	S	L	G	V
Secondary structure
SS confidence
Disorder																																						?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		L	A	Q	Q	L	Q	N	A	K	L	Q	A	D	A	A	H	S	A	L	K	Q	S	D	D	L	K	P	V	F	D	Q	A	F	T	K	V	V	T	T	P	A	D	A	L	Q	P	L	I	P	A	A	Q	T	F	T	Q	Q	L	V	M
Secondary structure
SS confidence
Disorder													?					?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.
Sequence		V	G	D	Y	I	A	Q	Q	G	T	Q	V	S	F	V	A	N	G	I	Q	F	P	T	S	Q	Q	A	S	E	Y	N	K	L	I	A	P	L	P	A	Q	H	Q	A	F	N	Q	A	W	T	T	A	V	T	A	T	Q
Secondary structure
SS confidence
Disorder																?		?	?		?	?	?																	?	?															?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 2c8s chain A domain 1 Region: 31 - 69 Aligned: 39 Modelled: 39 Confidence: 60.6% Identity: 18% Fold: Cytochrome c Superfamily: Cytochrome c Family: monodomain cytochrome c Phyre2 PDB 1fc2 chain C Region: 30 - 63 Aligned: 25 Modelled: 34 Confidence: 59.7% Identity: 36% Fold: immunoglobulin/albumin-binding domain-like Superfamily: Bacterial immunoglobulin/albumin-binding domains Family: Immunoglobulin-binding protein A modules Phyre2 PDB 1lp1 chain B Region: 31 - 63 Aligned: 24 Modelled: 33 Confidence: 59.3% Identity: 38% Fold: immunoglobulin/albumin-binding domain-like Superfamily: Bacterial immunoglobulin/albumin-binding domains Family: Immunoglobulin-binding protein A modules Phyre2 PDB 2d0w chain A Region: 31 - 66 Aligned: 36 Modelled: 36 Confidence: 49.7% Identity: 25% PDB header:electron transport Chain: A: PDB Molecule:cytochrome cl; PDBTitle: crystal structure of cytochrome cl from hyphomicrobium2 denitrificans Phyre2 PDB 1dee chain G Region: 32 - 63 Aligned: 23 Modelled: 32 Confidence: 47.0% Identity: 43% Fold: immunoglobulin/albumin-binding domain-like Superfamily: Bacterial immunoglobulin/albumin-binding domains Family: Immunoglobulin-binding protein A modules Phyre2 PDB 1zdb chain A Region: 31 - 63 Aligned: 24 Modelled: 33 Confidence: 47.0% Identity: 25% PDB header:igg binding domain Chain: A: PDB Molecule:mini protein a domain, z38; PDBTitle: phage-selected mini protein a domain, z38, nmr, minimized2 mean structure Phyre2 PDB 2jwd chain A domain 1 Region: 27 - 63 Aligned: 28 Modelled: 37 Confidence: 46.5% Identity: 32% Fold: immunoglobulin/albumin-binding domain-like Superfamily: Bacterial immunoglobulin/albumin-binding domains Family: Immunoglobulin-binding protein A modules Phyre2 PDB 2gc4 chain D domain 1 Region: 31 - 66 Aligned: 36 Modelled: 36 Confidence: 42.3% Identity: 22% Fold: Cytochrome c Superfamily: Cytochrome c Family: monodomain cytochrome c Phyre2 PDB 1lp1 chain A Region: 31 - 63 Aligned: 24 Modelled: 33 Confidence: 42.3% Identity: 33% Fold: immunoglobulin/albumin-binding domain-like Superfamily: Bacterial immunoglobulin/albumin-binding domains Family: Immunoglobulin-binding protein A modules Phyre2 PDB 2g38 chain A Region: 63 - 87 Aligned: 25 Modelled: 25 Confidence: 38.3% Identity: 16% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:pe family protein; PDBTitle: a pe/ppe protein complex from mycobacterium tuberculosis Phyre2 PDB 2g38 chain A domain 1 Region: 63 - 87 Aligned: 25 Modelled: 25 Confidence: 38.3% Identity: 16% Fold: Ferritin-like Superfamily: PE/PPE dimer-like Family: PE Phyre2 PDB 3fgg chain A Region: 69 - 152 Aligned: 81 Modelled: 81 Confidence: 36.6% Identity: 20% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:uncharacterized protein bce2196; PDBTitle: crystal structure of putative ecf-type sigma factor negative effector2 from bacillus cereus Phyre2 PDB 3lue chain P Region: 30 - 58 Aligned: 29 Modelled: 29 Confidence: 31.9% Identity: 24% PDB header:structural protein Chain: P: PDB Molecule:alpha-actinin-3; PDBTitle: model of alpha-actinin ch1 bound to f-actin Phyre2 PDB 1edl chain A Region: 30 - 63 Aligned: 25 Modelled: 34 Confidence: 24.8% Identity: 44% Fold: immunoglobulin/albumin-binding domain-like Superfamily: Bacterial immunoglobulin/albumin-binding domains Family: Immunoglobulin-binding protein A modules Phyre2 PDB 2iy5 chain A domain 1 Region: 38 - 68 Aligned: 31 Modelled: 31 Confidence: 20.3% Identity: 13% Fold: Long alpha-hairpin Superfamily: tRNA-binding arm Family: Phenylalanyl-tRNA synthetase (PheRS) Phyre2 PDB 1ojq chain A Region: 54 - 76 Aligned: 23 Modelled: 23 Confidence: 18.0% Identity: 4% Fold: ADP-ribosylation Superfamily: ADP-ribosylation Family: ADP-ribosylating toxins Phyre2 PDB 3uc2 chain A Region: 56 - 70 Aligned: 15 Modelled: 15 Confidence: 15.8% Identity: 27% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:hypothetical protein with immunoglobulin-like fold; PDBTitle: crystal structure of a hypothetical protein with immunoglobulin-like2 fold (pa0388) from pseudomonas aeruginosa pao1 at 2.09 a resolution Phyre2 PDB 2xgl chain B Region: 26 - 47 Aligned: 21 Modelled: 22 Confidence: 14.2% Identity: 33% PDB header:antibiotic Chain: B: PDB Molecule:colicin-m immunity protein; PDBTitle: the x-ray structure of the escherichia coli colicin m immunity2 protein demonstrates the presence of a disulphide bridge, which is3 functionally essential Phyre2 PDB 1hyn chain Q Region: 14 - 61 Aligned: 48 Modelled: 48 Confidence: 13.9% Identity: 27% PDB header:membrane protein Chain: Q: PDB Molecule:band 3 anion transport protein; PDBTitle: crystal structure of the cytoplasmic domain of human2 erythrocyte band-3 protein Phyre2 PDB 1g5g chain A Region: 71 - 132 Aligned: 50 Modelled: 54 Confidence: 13.8% Identity: 24% PDB header:viral protein Chain: A: PDB Molecule:fusion protein; PDBTitle: fragment of fusion protein from newcastle disease virus Phyre2 1 d2c8sa1 2 d1fc2c_ 3 d1lp1b_ 4 c2d0wA_ 5 d1deeg_ 6 c1zdbA_ 7 d2jwda1 8 d2gc4d1 9 d1lp1a_ 10 c2g38A_ 11 d2g38a1 12 c3fggA_ 13 c3lueP_ 14 d1edla_ 15 d2iy5a1 16 d1ojqa_ 17 c3uc2A_ 18 c2xglB_ 19 c1hynQ_ 20 c1g5gA_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 d2c8sa1 60.6 18 Fold:Cytochrome c Superfamily:Cytochrome c Family:monodomain cytochrome c 2 d1fc2c_ 59.7 36 Fold:immunoglobulin/albumin-binding domain-like Superfamily:Bacterial immunoglobulin/albumin-binding domains Family:Immunoglobulin-binding protein A modules 3 d1lp1b_ 59.3 38 Fold:immunoglobulin/albumin-binding domain-like Superfamily:Bacterial immunoglobulin/albumin-binding domains Family:Immunoglobulin-binding protein A modules 4 c2d0wA_ 49.7 25 PDB header:electron transport Chain: A: PDB Molecule:cytochrome cl; PDBTitle: crystal structure of cytochrome cl from hyphomicrobium2 denitrificans 5 d1deeg_ 47.0 43 Fold:immunoglobulin/albumin-binding domain-like Superfamily:Bacterial immunoglobulin/albumin-binding domains Family:Immunoglobulin-binding protein A modules 6 c1zdbA_ 47.0 25 PDB header:igg binding domain Chain: A: PDB Molecule:mini protein a domain, z38; PDBTitle: phage-selected mini protein a domain, z38, nmr, minimized2 mean structure 7 d2jwda1 46.5 32 Fold:immunoglobulin/albumin-binding domain-like Superfamily:Bacterial immunoglobulin/albumin-binding domains Family:Immunoglobulin-binding protein A modules 8 d2gc4d1 42.3 22 Fold:Cytochrome c Superfamily:Cytochrome c Family:monodomain cytochrome c 9 d1lp1a_ 42.3 33 Fold:immunoglobulin/albumin-binding domain-like Superfamily:Bacterial immunoglobulin/albumin-binding domains Family:Immunoglobulin-binding protein A modules 10 c2g38A_ 38.3 16 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:pe family protein; PDBTitle: a pe/ppe protein complex from mycobacterium tuberculosis 11 d2g38a1 38.3 16 Fold:Ferritin-like Superfamily:PE/PPE dimer-like Family:PE 12 c3fggA_ 36.6 20 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:uncharacterized protein bce2196; PDBTitle: crystal structure of putative ecf-type sigma factor negative effector2 from bacillus cereus 13 c3lueP_ 31.9 24 PDB header:structural protein Chain: P: PDB Molecule:alpha-actinin-3; PDBTitle: model of alpha-actinin ch1 bound to f-actin 14 d1edla_ 24.8 44 Fold:immunoglobulin/albumin-binding domain-like Superfamily:Bacterial immunoglobulin/albumin-binding domains Family:Immunoglobulin-binding protein A modules 15 d2iy5a1 20.3 13 Fold:Long alpha-hairpin Superfamily:tRNA-binding arm Family:Phenylalanyl-tRNA synthetase (PheRS) 16 d1ojqa_ 18.0 4 Fold:ADP-ribosylation Superfamily:ADP-ribosylation Family:ADP-ribosylating toxins 17 c3uc2A_ 15.8 27 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:hypothetical protein with immunoglobulin-like fold; PDBTitle: crystal structure of a hypothetical protein with immunoglobulin-like2 fold (pa0388) from pseudomonas aeruginosa pao1 at 2.09 a resolution 18 c2xglB_ 14.2 33 PDB header:antibiotic Chain: B: PDB Molecule:colicin-m immunity protein; PDBTitle: the x-ray structure of the escherichia coli colicin m immunity2 protein demonstrates the presence of a disulphide bridge, which is3 functionally essential 19 c1hynQ_ 13.9 27 PDB header:membrane protein Chain: Q: PDB Molecule:band 3 anion transport protein; PDBTitle: crystal structure of the cytoplasmic domain of human2 erythrocyte band-3 protein 20 c1g5gA_ 13.8 24 PDB header:viral protein Chain: A: PDB Molecule:fusion protein; PDBTitle: fragment of fusion protein from newcastle disease virus 21 d1r45a_ not modelled 12.1 13 Fold:ADP-ribosylation Superfamily:ADP-ribosylation Family:ADP-ribosylating toxins 22 d1fqva1 not modelled 11.8 38 Fold:F-box domain Superfamily:F-box domain Family:F-box domain 23 c2kv5A_ not modelled 11.7 36 PDB header:toxin Chain: A: PDB Molecule:putative uncharacterized protein rnai; PDBTitle: solution structure of the par toxin fst in dpc micelles 24 c2yskA_ not modelled 10.9 12 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:hypothetical protein ttha1432; PDBTitle: crystal structure of a hypothetical protein ttha1432 from thermus2 thermophilus 25 d1qn2a_ not modelled 10.6 13 Fold:Cytochrome c Superfamily:Cytochrome c Family:monodomain cytochrome c 26 d1qhma_ not modelled 10.4 14 Fold:PFL-like glycyl radical enzymes Superfamily:PFL-like glycyl radical enzymes Family:PFL-like 27 c2dbhA_ not modelled 10.3 22 PDB header:signaling protein Chain: A: PDB Molecule:tumor necrosis factor receptor superfamily PDBTitle: solution structure of the carboxyl-terminal card-like2 domain in human tnfr-related death receptor-6 28 d1eiya1 not modelled 10.3 12 Fold:Long alpha-hairpin Superfamily:tRNA-binding arm Family:Phenylalanyl-tRNA synthetase (PheRS) 29 d1y9ua_ not modelled 10.1 15 Fold:Periplasmic binding protein-like II Superfamily:Periplasmic binding protein-like II Family:Phosphate binding protein-like 30 c3ermD_ not modelled 10.0 16 PDB header:structural genomics, unknown function Chain: D: PDB Molecule:uncharacterized conserved protein; PDBTitle: the crystal structure of a conserved protein with unknown function2 from pseudomonas syringae pv. tomato str. dc3000 31 d4icba_ not modelled 9.7 31 Fold:EF Hand-like Superfamily:EF-hand Family:Calbindin D9K 32 c3swyB_ not modelled 8.8 18 PDB header:transport protein Chain: B: PDB Molecule:cyclic nucleotide-gated cation channel alpha-3; PDBTitle: cnga3 626-672 containing clz domain 33 d1j3sa_ not modelled 8.3 21 Fold:Cytochrome c Superfamily:Cytochrome c Family:monodomain cytochrome c 34 c2x7aB_ not modelled 8.0 22 PDB header:immune system Chain: B: PDB Molecule:bone marrow stromal antigen 2; PDBTitle: structural basis of hiv-1 tethering to membranes by the2 bst2-tetherin ectodomain 35 c2kwhA_ not modelled 7.6 27 PDB header:transport protein Chain: A: PDB Molecule:rala-binding protein 1; PDBTitle: ral binding domain of rlip76 (ralbp1) 36 d1sh5a1 not modelled 7.6 10 Fold:CH domain-like Superfamily:Calponin-homology domain, CH-domain Family:Calponin-homology domain, CH-domain 37 d1hynp_ not modelled 7.2 24 Fold:Phoshotransferase/anion transport protein Superfamily:Phoshotransferase/anion transport protein Family:Anion transport protein, cytoplasmic domain 38 d1y4ta_ not modelled 7.1 26 Fold:Periplasmic binding protein-like II Superfamily:Periplasmic binding protein-like II Family:Phosphate binding protein-like 39 c2zykA_ not modelled 7.0 14 PDB header:sugar binding protein Chain: A: PDB Molecule:solute-binding protein; PDBTitle: crystal structure of cyclo/maltodextrin-binding protein2 complexed with gamma-cyclodextrin 40 c3bw8B_ not modelled 7.0 17 PDB header:transferase Chain: B: PDB Molecule:mono-adp-ribosyltransferase c3; PDBTitle: crystal structure of the clostridium limosum c3 exoenzyme 41 c3d0wD_ not modelled 6.9 36 PDB header:structural genomics, unknown function Chain: D: PDB Molecule:yflh protein; PDBTitle: crystal structure of yflh protein from bacillus subtilis.2 northeast structural genomics consortium target sr326 42 d1lmsa_ not modelled 6.8 21 Fold:Cytochrome c Superfamily:Cytochrome c Family:monodomain cytochrome c 43 d1ccra_ not modelled 6.8 23 Fold:Cytochrome c Superfamily:Cytochrome c Family:monodomain cytochrome c 44 d1l8ya_ not modelled 6.7 42 Fold:HMG-box Superfamily:HMG-box Family:HMG-box 45 d1nmla2 not modelled 6.4 28 Fold:Cytochrome c Superfamily:Cytochrome c Family:Di-heme cytochrome c peroxidase 46 c3gdzA_ not modelled 6.3 18 PDB header:ligase Chain: A: PDB Molecule:arginyl-trna synthetase; PDBTitle: crystal structure of arginyl-trna synthetase from klebsiella2 pneumoniae subsp. pneumoniae 47 d3ctda1 not modelled 6.2 0 Fold:post-AAA+ oligomerization domain-like Superfamily:post-AAA+ oligomerization domain-like Family:MgsA/YrvN C-terminal domain-like 48 c2pt1A_ not modelled 6.0 24 PDB header:metal transport Chain: A: PDB Molecule:iron transport protein; PDBTitle: futa1 synechocystis pcc 6803 49 c3oeoD_ not modelled 6.0 78 PDB header:signaling protein Chain: D: PDB Molecule:spheroplast protein y; PDBTitle: the crystal structure e. coli spy 50 c1sj8A_ not modelled 5.9 12 PDB header:structural protein Chain: A: PDB Molecule:talin 1; PDBTitle: crystal structure of talin residues 482-789 51 c1sszA_ not modelled 5.8 33 PDB header:surface active protein Chain: A: PDB Molecule:pulmonary surfactant-associated protein b; PDBTitle: conformational mapping of mini-b: an n-terminal/c-terminal2 construct of surfactant protein b using 13c-enhanced3 fourier transform infrared (ftir) spectroscopy 52 d1aoaa1 not modelled 5.7 8 Fold:CH domain-like Superfamily:Calponin-homology domain, CH-domain Family:Calponin-homology domain, CH-domain 53 c3m97X_ not modelled 5.7 21 PDB header:electron transport Chain: X: PDB Molecule:cytochrome c-552; PDBTitle: structure of the soluble domain of cytochrome c552 with its flexible2 linker segment from paracoccus denitrificans 54 d1ycca_ not modelled 5.6 21 Fold:Cytochrome c Superfamily:Cytochrome c Family:monodomain cytochrome c 55 c2z6vA_ not modelled 5.6 14 PDB header:unknown function Chain: A: PDB Molecule:putative uncharacterized protein; PDBTitle: crystal structure of sulfotransferase stf9 from2 mycobacterium avium 56 d1hywa_ not modelled 5.3 36 Fold:gpW/XkdW-like Superfamily:Head-to-tail joining protein W, gpW Family:Head-to-tail joining protein W, gpW 57 d1cl1a_ not modelled 5.2 35 Fold:PLP-dependent transferase-like Superfamily:PLP-dependent transferases Family:Cystathionine synthase-like 58 d1hroa_ not modelled 5.2 23 Fold:Cytochrome c Superfamily:Cytochrome c Family:monodomain cytochrome c 59 c2qk1A_ not modelled 5.2 13 PDB header:protein binding Chain: A: PDB Molecule:protein stu2; PDBTitle: structural basis of microtubule plus end tracking by2 xmap215, clip-170 and eb1

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0