Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	S	T	P	D	N	R	S	V	N	F	F	S	L	F	R	R	G	Q	H	Y	S	K	T	W	P	L	E	K	R	L	A	P	V	F	V	E	N	R	V	I	K	M	T	R	Y	A	I	R	F	M	P	P	I	A	V	F	T	L	C
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		W	Q	I	A	L	G	G	Q	L	G	P	A	V	A	T	A	L	F	A	L	S	L	P	M	Q	G	L	W	W	L	G	K	R	S	V	T	P	L	P	P	A	I	L	N	W	F	Y	E	V	R	G	K	L	Q	E	S	G	Q	V	L
Secondary structure
SS confidence
Disorder																																								?																	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.
Sequence		A	P	V	E	G	K	P	D	Y	Q	A	L	A	D	T	L	K	R	A	F	K	Q	L	D	K	T	F	L	D	D	L
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?		?																						?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 2l9u chain A Region: 69 - 96 Aligned: 28 Modelled: 28 Confidence: 31.7% Identity: 25% PDB header:membrane protein Chain: A: PDB Molecule:receptor tyrosine-protein kinase erbb-3; PDBTitle: spatial structure of dimeric erbb3 transmembrane domain Phyre2 PDB 3lwf chain D Region: 34 - 56 Aligned: 23 Modelled: 23 Confidence: 23.2% Identity: 17% PDB header:transcription regulator Chain: D: PDB Molecule:putative transcriptional regulator; PDBTitle: crystal structure of putative transcriptional regulator (np_470886.1)2 from listeria innocua at 2.06 a resolution Phyre2 PDB 3tr7 chain A Region: 91 - 113 Aligned: 23 Modelled: 23 Confidence: 17.3% Identity: 35% PDB header:hydrolase Chain: A: PDB Molecule:uracil-dna glycosylase; PDBTitle: structure of a uracil-dna glycosylase (ung) from coxiella burnetii Phyre2 PDB 2oso chain A domain 1 Region: 87 - 117 Aligned: 28 Modelled: 29 Confidence: 15.8% Identity: 21% Fold: Ligand-binding domain in the NO signalling and Golgi transport Superfamily: Ligand-binding domain in the NO signalling and Golgi transport Family: MJ1460-like Phyre2 PDB 3ajb chain B Region: 131 - 146 Aligned: 16 Modelled: 16 Confidence: 14.3% Identity: 31% PDB header:protein transport Chain: B: PDB Molecule:peroxisomal biogenesis factor 19; PDBTitle: crystal structure of human pex3p in complex with n-terminal pex19p2 peptide Phyre2 PDB 3njc chain A Region: 87 - 99 Aligned: 13 Modelled: 12 Confidence: 13.8% Identity: 31% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:yslb protein; PDBTitle: crystal structure of the yslb protein from bacillus subtilis.2 northeast structural genomics consortium target sr460. Phyre2 PDB 1ioj chain A Region: 95 - 116 Aligned: 22 Modelled: 22 Confidence: 13.2% Identity: 27% PDB header:apolipoprotein Chain: A: PDB Molecule:apoc-i; PDBTitle: human apolipoprotein c-i, nmr, 18 structures Phyre2 PDB 2qtv chain A domain 3 Region: 95 - 150 Aligned: 56 Modelled: 56 Confidence: 12.9% Identity: 9% Fold: vWA-like Superfamily: vWA-like Family: Trunk domain of Sec23/24 Phyre2 PDB 3sib chain A Region: 92 - 146 Aligned: 40 Modelled: 40 Confidence: 12.3% Identity: 13% PDB header:dna binding protein Chain: A: PDB Molecule:ure3-bp sequence specific dna binding protein; PDBTitle: crystal structure of ure3-binding protein, wild-type Phyre2 PDB 1taf chain B Region: 37 - 51 Aligned: 15 Modelled: 15 Confidence: 12.2% Identity: 20% Fold: Histone-fold Superfamily: Histone-fold Family: TBP-associated factors, TAFs Phyre2 PDB 1kcf chain B Region: 84 - 95 Aligned: 12 Modelled: 12 Confidence: 11.6% Identity: 8% PDB header:hydrolase Chain: B: PDB Molecule:hypothetical 30.2 kd protein c25g10.02 in PDBTitle: crystal structure of the yeast mitochondrial holliday2 junction resolvase, ydc2 Phyre2 PDB 3eug chain A Region: 93 - 111 Aligned: 19 Modelled: 19 Confidence: 10.4% Identity: 32% Fold: Uracil-DNA glycosylase-like Superfamily: Uracil-DNA glycosylase-like Family: Uracil-DNA glycosylase Phyre2 PDB 1okb chain A Region: 90 - 111 Aligned: 22 Modelled: 22 Confidence: 10.0% Identity: 23% Fold: Uracil-DNA glycosylase-like Superfamily: Uracil-DNA glycosylase-like Family: Uracil-DNA glycosylase Phyre2 PDB 2j8x chain A domain 1 Region: 90 - 111 Aligned: 22 Modelled: 22 Confidence: 9.6% Identity: 23% Fold: Uracil-DNA glycosylase-like Superfamily: Uracil-DNA glycosylase-like Family: Uracil-DNA glycosylase Phyre2 PDB 1p5k chain A Region: 137 - 147 Aligned: 11 Modelled: 11 Confidence: 9.5% Identity: 55% PDB header:ribosome Chain: A: PDB Molecule:19-mer peptide from 50s ribosomal protein l1; PDBTitle: hp (2-20) substitution ser to leu11 modification in sds-d252 micelles Phyre2 PDB 1lau chain E Region: 93 - 111 Aligned: 19 Modelled: 19 Confidence: 9.2% Identity: 32% Fold: Uracil-DNA glycosylase-like Superfamily: Uracil-DNA glycosylase-like Family: Uracil-DNA glycosylase Phyre2 PDB 1vfg chain A domain 1 Region: 39 - 50 Aligned: 12 Modelled: 12 Confidence: 9.0% Identity: 33% Fold: Poly A polymerase C-terminal region-like Superfamily: Poly A polymerase C-terminal region-like Family: Poly A polymerase C-terminal region-like Phyre2 PDB 3ls1 chain A Region: 108 - 146 Aligned: 38 Modelled: 39 Confidence: 8.7% Identity: 18% PDB header:photosynthesis Chain: A: PDB Molecule:sll1638 protein; PDBTitle: crystal structure of cyanobacterial psbq from synechocystis2 sp. pcc 6803 complexed with zn2+ Phyre2 PDB 3cxm chain A Region: 90 - 111 Aligned: 22 Modelled: 22 Confidence: 8.0% Identity: 27% PDB header:hydrolase Chain: A: PDB Molecule:uracil-dna glycosylase; PDBTitle: leishmania naiffi uracil-dna glycosylase in complex with 5-bromouracil Phyre2 PDB 2hxm chain A domain 1 Region: 90 - 111 Aligned: 22 Modelled: 22 Confidence: 7.6% Identity: 23% Fold: Uracil-DNA glycosylase-like Superfamily: Uracil-DNA glycosylase-like Family: Uracil-DNA glycosylase Phyre2 1 c2l9uA_ 2 c3lwfD_ 3 c3tr7A_ 4 d2osoa1 5 c3ajbB_ 6 c3njcA_ 7 c1iojA_ 8 d2qtva3 9 c3sibA_ 10 d1tafb_ 11 c1kcfB_ 12 d3euga_ 13 d1okba_ 14 d2j8xa1 15 c1p5kA_ 16 d1laue_ 17 d1vfga1 18 c3ls1A_ 19 c3cxmA_ 20 d2hxma1 21 22 23 24 25 26 27 28 29 30 31 32

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 c2l9uA_ 31.7 25 PDB header:membrane protein Chain: A: PDB Molecule:receptor tyrosine-protein kinase erbb-3; PDBTitle: spatial structure of dimeric erbb3 transmembrane domain 2 c3lwfD_ 23.2 17 PDB header:transcription regulator Chain: D: PDB Molecule:putative transcriptional regulator; PDBTitle: crystal structure of putative transcriptional regulator (np_470886.1)2 from listeria innocua at 2.06 a resolution 3 c3tr7A_ 17.3 35 PDB header:hydrolase Chain: A: PDB Molecule:uracil-dna glycosylase; PDBTitle: structure of a uracil-dna glycosylase (ung) from coxiella burnetii 4 d2osoa1 15.8 21 Fold:Ligand-binding domain in the NO signalling and Golgi transport Superfamily:Ligand-binding domain in the NO signalling and Golgi transport Family:MJ1460-like 5 c3ajbB_ 14.3 31 PDB header:protein transport Chain: B: PDB Molecule:peroxisomal biogenesis factor 19; PDBTitle: crystal structure of human pex3p in complex with n-terminal pex19p2 peptide 6 c3njcA_ 13.8 31 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:yslb protein; PDBTitle: crystal structure of the yslb protein from bacillus subtilis.2 northeast structural genomics consortium target sr460. 7 c1iojA_ 13.2 27 PDB header:apolipoprotein Chain: A: PDB Molecule:apoc-i; PDBTitle: human apolipoprotein c-i, nmr, 18 structures 8 d2qtva3 12.9 9 Fold:vWA-like Superfamily:vWA-like Family:Trunk domain of Sec23/24 9 c3sibA_ 12.3 13 PDB header:dna binding protein Chain: A: PDB Molecule:ure3-bp sequence specific dna binding protein; PDBTitle: crystal structure of ure3-binding protein, wild-type 10 d1tafb_ 12.2 20 Fold:Histone-fold Superfamily:Histone-fold Family:TBP-associated factors, TAFs 11 c1kcfB_ 11.6 8 PDB header:hydrolase Chain: B: PDB Molecule:hypothetical 30.2 kd protein c25g10.02 in PDBTitle: crystal structure of the yeast mitochondrial holliday2 junction resolvase, ydc2 12 d3euga_ 10.4 32 Fold:Uracil-DNA glycosylase-like Superfamily:Uracil-DNA glycosylase-like Family:Uracil-DNA glycosylase 13 d1okba_ 10.0 23 Fold:Uracil-DNA glycosylase-like Superfamily:Uracil-DNA glycosylase-like Family:Uracil-DNA glycosylase 14 d2j8xa1 9.6 23 Fold:Uracil-DNA glycosylase-like Superfamily:Uracil-DNA glycosylase-like Family:Uracil-DNA glycosylase 15 c1p5kA_ 9.5 55 PDB header:ribosome Chain: A: PDB Molecule:19-mer peptide from 50s ribosomal protein l1; PDBTitle: hp (2-20) substitution ser to leu11 modification in sds-d252 micelles 16 d1laue_ 9.2 32 Fold:Uracil-DNA glycosylase-like Superfamily:Uracil-DNA glycosylase-like Family:Uracil-DNA glycosylase 17 d1vfga1 9.0 33 Fold:Poly A polymerase C-terminal region-like Superfamily:Poly A polymerase C-terminal region-like Family:Poly A polymerase C-terminal region-like 18 c3ls1A_ 8.7 18 PDB header:photosynthesis Chain: A: PDB Molecule:sll1638 protein; PDBTitle: crystal structure of cyanobacterial psbq from synechocystis2 sp. pcc 6803 complexed with zn2+ 19 c3cxmA_ 8.0 27 PDB header:hydrolase Chain: A: PDB Molecule:uracil-dna glycosylase; PDBTitle: leishmania naiffi uracil-dna glycosylase in complex with 5-bromouracil 20 d2hxma1 7.6 23 Fold:Uracil-DNA glycosylase-like Superfamily:Uracil-DNA glycosylase-like Family:Uracil-DNA glycosylase 21 c2booA_ not modelled 7.5 23 PDB header:hydrolase Chain: A: PDB Molecule:uracil-dna glycosylase; PDBTitle: the crystal structure of uracil-dna n-glycosylase (ung)2 from deinococcus radiodurans. 22 d1oeyj_ not modelled 7.2 22 Fold:beta-Grasp (ubiquitin-like) Superfamily:CAD & PB1 domains Family:PB1 domain 23 d1pd0a3 not modelled 6.3 9 Fold:vWA-like Superfamily:vWA-like Family:Trunk domain of Sec23/24 24 d2gykb1 not modelled 6.3 27 Fold:His-Me finger endonucleases Superfamily:His-Me finger endonucleases Family:HNH-motif 25 c3eh2B_ not modelled 6.1 7 PDB header:protein transport Chain: B: PDB Molecule:protein transport protein sec24c; PDBTitle: crystal structure of the human copii-coat protein sec24c 26 c3op5B_ not modelled 6.0 30 PDB header:transferase Chain: B: PDB Molecule:serine/threonine-protein kinase vrk1; PDBTitle: human vaccinia-related kinase 1 27 d1miwa1 not modelled 6.0 17 Fold:Poly A polymerase C-terminal region-like Superfamily:Poly A polymerase C-terminal region-like Family:Poly A polymerase C-terminal region-like 28 c2akhZ_ not modelled 5.9 25 PDB header:protein transport Chain: Z: PDB Molecule:preprotein translocase sece subunit; PDBTitle: normal mode-based flexible fitted coordinates of a non-2 translocating secyeg protein-conducting channel into the3 cryo-em map of a secyeg-nascent chain-70s ribosome complex4 from e. coli 29 d1csna_ not modelled 5.7 24 Fold:Protein kinase-like (PK-like) Superfamily:Protein kinase-like (PK-like) Family:Protein kinases, catalytic subunit 30 c3dinD_ not modelled 5.6 50 PDB header:membrane protein, protein transport Chain: D: PDB Molecule:preprotein translocase subunit sece; PDBTitle: crystal structure of the protein-translocation complex formed by the2 secy channel and the seca atpase 31 d1rbli_ not modelled 5.5 56 Fold:RuBisCO, small subunit Superfamily:RuBisCO, small subunit Family:RuBisCO, small subunit 32 d1kcfa2 not modelled 5.2 8 Fold:Ribonuclease H-like motif Superfamily:Ribonuclease H-like Family:Mitochondrial resolvase ydc2 catalytic domain

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

Transmembrane helix prediction

Transmembrane helices have been predicted in your sequence to adopt the topology shown below

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0