Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	K	K	I	I	C	L	V	I	T	L	L	M	T	L	P	V	Y	A	K	L	T	A	H	E	E	A	R	I	N	A	M	L	E	G	L	A	Q	K	K	D	L	I	F	V	R	N	G	D	E	H	T	C	Y	E	A	V	S	H	L
Secondary structure
SS confidence
Disorder		?	?	?															?	?	?	?	?	?	?																												?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		R	L	K	L	G	N	T	R	N	R	I	D	T	A	E	Q	F	I	D	K	V	A	S	S	S	S	I	T	G	K	P	Y	I	V	K	I	P	G	K	S	D	E	N	A	Q	P	F	L	H	A	L	I	A	Q	T	D	K	T	V	P
Secondary structure
SS confidence
Disorder									?	?	?		?	?											?	?													?	?	?	?	?	?														?	?	?	?
Disorder confidence
		.	.	.	.
Sequence		A	E	G	N
Secondary structure
SS confidence
Disorder		?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 1t3u chain A Region: 86 - 123 Aligned: 38 Modelled: 38 Confidence: 81.3% Identity: 21% Fold: Cell division protein ZapA-like Superfamily: Cell division protein ZapA-like Family: Cell division protein ZapA-like Phyre2 PDB 2jtc chain A Region: 20 - 120 Aligned: 75 Modelled: 75 Confidence: 66.3% Identity: 13% PDB header:hydrolase Chain: A: PDB Molecule:streptopain; PDBTitle: 3d structure and backbone dynamics of spe b Phyre2 PDB 1dki chain A Region: 20 - 120 Aligned: 77 Modelled: 77 Confidence: 63.8% Identity: 16% Fold: Cysteine proteinases Superfamily: Cysteine proteinases Family: Papain-like Phyre2 PDB 1pvj chain A Region: 22 - 120 Aligned: 75 Modelled: 75 Confidence: 55.5% Identity: 16% Fold: Cysteine proteinases Superfamily: Cysteine proteinases Family: Papain-like Phyre2 PDB 1v61 chain A Region: 88 - 123 Aligned: 33 Modelled: 36 Confidence: 44.3% Identity: 21% Fold: PH domain-like barrel Superfamily: PH domain-like Family: Pleckstrin-homology domain (PH domain) Phyre2 PDB 1iyj chain B domain 5 Region: 57 - 84 Aligned: 28 Modelled: 28 Confidence: 37.9% Identity: 14% Fold: OB-fold Superfamily: Nucleic acid-binding proteins Family: Single strand DNA-binding domain, SSB Phyre2 PDB 1miu chain A domain 5 Region: 59 - 84 Aligned: 26 Modelled: 26 Confidence: 28.7% Identity: 15% Fold: OB-fold Superfamily: Nucleic acid-binding proteins Family: Single strand DNA-binding domain, SSB Phyre2 PDB 3hnw chain B Region: 87 - 120 Aligned: 34 Modelled: 34 Confidence: 15.9% Identity: 12% PDB header:structural genomics, unknown function Chain: B: PDB Molecule:uncharacterized protein; PDBTitle: crystal structure of a basic coiled-coil protein of unknown function2 from eubacterium eligens atcc 27750 Phyre2 PDB 3bb7 chain A Region: 20 - 120 Aligned: 75 Modelled: 75 Confidence: 15.1% Identity: 12% PDB header:hydrolase Chain: A: PDB Molecule:interpain a; PDBTitle: structure of prevotella intermedia prointerpain a fragment 39-3592 (mutant c154a) Phyre2 PDB 1o5h chain A Region: 73 - 89 Aligned: 17 Modelled: 17 Confidence: 14.9% Identity: 29% Fold: Methenyltetrahydrofolate cyclohydrolase-like Superfamily: Methenyltetrahydrofolate cyclohydrolase-like Family: Methenyltetrahydrofolate cyclohydrolase-like Phyre2 PDB 2eel chain A Region: 82 - 101 Aligned: 20 Modelled: 20 Confidence: 14.9% Identity: 30% PDB header:apoptosis Chain: A: PDB Molecule:cell death activator cide-a; PDBTitle: solution structure of the cide-n domain of human cell death2 activator cide-a Phyre2 PDB 1xau chain A Region: 78 - 97 Aligned: 20 Modelled: 20 Confidence: 11.6% Identity: 25% Fold: Immunoglobulin-like beta-sandwich Superfamily: Immunoglobulin Family: I set domains Phyre2 PDB 3ln3 chain A Region: 48 - 94 Aligned: 47 Modelled: 47 Confidence: 11.3% Identity: 23% PDB header:oxidoreductase Chain: A: PDB Molecule:dihydrodiol dehydrogenase; PDBTitle: crystal structure of putative reductase (np_038806.2) from2 mus musculus at 1.18 a resolution Phyre2 PDB 2p4v chain A Region: 47 - 74 Aligned: 28 Modelled: 28 Confidence: 10.6% Identity: 7% PDB header:transcription Chain: A: PDB Molecule:transcription elongation factor greb; PDBTitle: crystal structure of the transcript cleavage factor, greb2 at 2.6a resolution Phyre2 PDB 2ae8 chain A domain 2 Region: 87 - 94 Aligned: 8 Modelled: 8 Confidence: 10.1% Identity: 50% Fold: Ribosomal protein S5 domain 2-like Superfamily: Ribosomal protein S5 domain 2-like Family: Imidazole glycerol phosphate dehydratase Phyre2 PDB 1rhy chain A domain 2 Region: 87 - 94 Aligned: 8 Modelled: 8 Confidence: 10.1% Identity: 38% Fold: Ribosomal protein S5 domain 2-like Superfamily: Ribosomal protein S5 domain 2-like Family: Imidazole glycerol phosphate dehydratase Phyre2 PDB 1mhq chain A Region: 42 - 87 Aligned: 45 Modelled: 46 Confidence: 10.0% Identity: 18% Fold: alpha-alpha superhelix Superfamily: ENTH/VHS domain Family: VHS domain Phyre2 PDB 3zyq chain A Region: 42 - 122 Aligned: 67 Modelled: 81 Confidence: 9.6% Identity: 15% PDB header:signaling Chain: A: PDB Molecule:hepatocyte growth factor-regulated tyrosine kinase PDBTitle: crystal structure of the tandem vhs and fyve domains of hepatocyte2 growth factor-regulated tyrosine kinase substrate (hgs-hrs) at 1.483 a resolution Phyre2 PDB 3cxb chain B Region: 89 - 118 Aligned: 28 Modelled: 30 Confidence: 9.4% Identity: 7% PDB header:signaling protein Chain: B: PDB Molecule:pleckstrin homology domain-containing family m PDBTitle: crystal structure of sifa and skip Phyre2 PDB 2f1d chain A domain 2 Region: 87 - 94 Aligned: 8 Modelled: 8 Confidence: 9.2% Identity: 38% Fold: Ribosomal protein S5 domain 2-like Superfamily: Ribosomal protein S5 domain 2-like Family: Imidazole glycerol phosphate dehydratase Phyre2 1 d1t3ua_ 2 c2jtcA_ 3 d1dkia_ 4 d1pvja_ 5 d1v61a_ 6 d1iyjb5 7 d1miua5 8 c3hnwB_ 9 c3bb7A_ 10 d1o5ha_ 11 c2eelA_ 12 d1xaua_ 13 c3ln3A_ 14 c2p4vA_ 15 d2ae8a2 16 d1rhya2 17 d1mhqa_ 18 c3zyqA_ 19 c3cxbB_ 20 d2f1da2 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 d1t3ua_ 81.3 21 Fold:Cell division protein ZapA-like Superfamily:Cell division protein ZapA-like Family:Cell division protein ZapA-like 2 c2jtcA_ 66.3 13 PDB header:hydrolase Chain: A: PDB Molecule:streptopain; PDBTitle: 3d structure and backbone dynamics of spe b 3 d1dkia_ 63.8 16 Fold:Cysteine proteinases Superfamily:Cysteine proteinases Family:Papain-like 4 d1pvja_ 55.5 16 Fold:Cysteine proteinases Superfamily:Cysteine proteinases Family:Papain-like 5 d1v61a_ 44.3 21 Fold:PH domain-like barrel Superfamily:PH domain-like Family:Pleckstrin-homology domain (PH domain) 6 d1iyjb5 37.9 14 Fold:OB-fold Superfamily:Nucleic acid-binding proteins Family:Single strand DNA-binding domain, SSB 7 d1miua5 28.7 15 Fold:OB-fold Superfamily:Nucleic acid-binding proteins Family:Single strand DNA-binding domain, SSB 8 c3hnwB_ 15.9 12 PDB header:structural genomics, unknown function Chain: B: PDB Molecule:uncharacterized protein; PDBTitle: crystal structure of a basic coiled-coil protein of unknown function2 from eubacterium eligens atcc 27750 9 c3bb7A_ 15.1 12 PDB header:hydrolase Chain: A: PDB Molecule:interpain a; PDBTitle: structure of prevotella intermedia prointerpain a fragment 39-3592 (mutant c154a) 10 d1o5ha_ 14.9 29 Fold:Methenyltetrahydrofolate cyclohydrolase-like Superfamily:Methenyltetrahydrofolate cyclohydrolase-like Family:Methenyltetrahydrofolate cyclohydrolase-like 11 c2eelA_ 14.9 30 PDB header:apoptosis Chain: A: PDB Molecule:cell death activator cide-a; PDBTitle: solution structure of the cide-n domain of human cell death2 activator cide-a 12 d1xaua_ 11.6 25 Fold:Immunoglobulin-like beta-sandwich Superfamily:Immunoglobulin Family:I set domains 13 c3ln3A_ 11.3 23 PDB header:oxidoreductase Chain: A: PDB Molecule:dihydrodiol dehydrogenase; PDBTitle: crystal structure of putative reductase (np_038806.2) from2 mus musculus at 1.18 a resolution 14 c2p4vA_ 10.6 7 PDB header:transcription Chain: A: PDB Molecule:transcription elongation factor greb; PDBTitle: crystal structure of the transcript cleavage factor, greb2 at 2.6a resolution 15 d2ae8a2 10.1 50 Fold:Ribosomal protein S5 domain 2-like Superfamily:Ribosomal protein S5 domain 2-like Family:Imidazole glycerol phosphate dehydratase 16 d1rhya2 10.1 38 Fold:Ribosomal protein S5 domain 2-like Superfamily:Ribosomal protein S5 domain 2-like Family:Imidazole glycerol phosphate dehydratase 17 d1mhqa_ 10.0 18 Fold:alpha-alpha superhelix Superfamily:ENTH/VHS domain Family:VHS domain 18 c3zyqA_ 9.6 15 PDB header:signaling Chain: A: PDB Molecule:hepatocyte growth factor-regulated tyrosine kinase PDBTitle: crystal structure of the tandem vhs and fyve domains of hepatocyte2 growth factor-regulated tyrosine kinase substrate (hgs-hrs) at 1.483 a resolution 19 c3cxbB_ 9.4 7 PDB header:signaling protein Chain: B: PDB Molecule:pleckstrin homology domain-containing family m PDBTitle: crystal structure of sifa and skip 20 d2f1da2 9.2 38 Fold:Ribosomal protein S5 domain 2-like Superfamily:Ribosomal protein S5 domain 2-like Family:Imidazole glycerol phosphate dehydratase 21 c2hj3A_ not modelled 9.0 22 PDB header:oxidoreductase Chain: A: PDB Molecule:sulfhydryl oxidase erv1p; PDBTitle: structure of the arabidopsis thaliana erv1 thiol oxidase 22 d1eg3a2 not modelled 8.8 21 Fold:EF Hand-like Superfamily:EF-hand Family:EF-hand modules in multidomain proteins 23 d1mjda_ not modelled 8.7 50 Fold:beta-Grasp (ubiquitin-like) Superfamily:Doublecortin (DC) Family:Doublecortin (DC) 24 d2p6va1 not modelled 8.7 27 Fold:TAFH domain-like Superfamily:TAFH domain-like Family:TAFH domain-like 25 d1f45b_ not modelled 8.6 18 Fold:4-helical cytokines Superfamily:4-helical cytokines Family:Long-chain cytokines 26 d1rxwa1 not modelled 8.5 26 Fold:SAM domain-like Superfamily:5' to 3' exonuclease, C-terminal subdomain Family:5' to 3' exonuclease, C-terminal subdomain 27 c2dnfA_ not modelled 8.4 30 PDB header:protein binding Chain: A: PDB Molecule:doublecortin domain-containing protein 2; PDBTitle: solution structure of rsgi ruh-062, a dcx domain from human 28 c3s6n2_ not modelled 8.0 33 PDB header:splicing Chain: 2: PDB Molecule:survival of motor neuron protein-interacting protein 1; PDB Fragment:gemin2-binding domain; PDBTitle: crystal structure of the gemin2-binding domain of smn, gemin2 in2 complex with smd1/d2/f/e/g from human 29 d1dj0a_ not modelled 8.0 25 Fold:Pseudouridine synthase Superfamily:Pseudouridine synthase Family:Pseudouridine synthase I TruA 30 d1mg4a_ not modelled 7.8 50 Fold:beta-Grasp (ubiquitin-like) Superfamily:Doublecortin (DC) Family:Doublecortin (DC) 31 d2aw2a1 not modelled 7.6 25 Fold:Immunoglobulin-like beta-sandwich Superfamily:Immunoglobulin Family:V set domains (antibody variable domain-like) 32 d1huna_ not modelled 7.5 0 Fold:IL8-like Superfamily:Interleukin 8-like chemokines Family:Interleukin 8-like chemokines 33 c3bbaB_ not modelled 7.4 14 PDB header:hydrolase Chain: B: PDB Molecule:interpain a; PDBTitle: structure of active wild-type prevotella intermedia interpain a2 cysteine protease 34 d1jr8a_ not modelled 7.3 14 Fold:Four-helical up-and-down bundle Superfamily:FAD-dependent thiol oxidase Family:FAD-dependent thiol oxidase 35 c2ae8C_ not modelled 7.3 50 PDB header:lyase Chain: C: PDB Molecule:imidazoleglycerol-phosphate dehydratase; PDBTitle: crystal structure of imidazoleglycerol-phosphate dehydratase from2 staphylococcus aureus subsp. aureus n315 36 d2cmda2 not modelled 7.1 21 Fold:LDH C-terminal domain-like Superfamily:LDH C-terminal domain-like Family:Lactate & malate dehydrogenases, C-terminal domain 37 c1rhyB_ not modelled 7.1 38 PDB header:lyase Chain: B: PDB Molecule:imidazole glycerol phosphate dehydratase; PDBTitle: crystal structure of imidazole glycerol phosphate dehydratase 38 d1dvpa1 not modelled 7.0 17 Fold:alpha-alpha superhelix Superfamily:ENTH/VHS domain Family:VHS domain 39 c1tg6G_ not modelled 7.0 14 PDB header:hydrolase Chain: G: PDB Molecule:putative atp-dependent clp protease proteolytic subunit; PDBTitle: crystallography and mutagenesis point to an essential role for the n-2 terminus of human mitochondrial clpp 40 d1gvpa_ not modelled 6.9 50 Fold:OB-fold Superfamily:Nucleic acid-binding proteins Family:Phage ssDNA-binding proteins 41 d1uf0a_ not modelled 6.8 45 Fold:beta-Grasp (ubiquitin-like) Superfamily:Doublecortin (DC) Family:Doublecortin (DC) 42 d1f5qb2 not modelled 6.8 38 Fold:Cyclin-like Superfamily:Cyclin-like Family:Cyclin 43 d2raxa1 not modelled 6.6 15 Fold:Inhibitor of apoptosis (IAP) repeat Superfamily:Inhibitor of apoptosis (IAP) repeat Family:Inhibitor of apoptosis (IAP) repeat 44 c2f1dP_ not modelled 6.6 38 PDB header:lyase Chain: P: PDB Molecule:imidazoleglycerol-phosphate dehydratase 1; PDBTitle: x-ray structure of imidazoleglycerol-phosphate dehydratase 45 d1v89a_ not modelled 6.6 15 Fold:PH domain-like barrel Superfamily:PH domain-like Family:Pleckstrin-homology domain (PH domain) 46 d1a77a1 not modelled 6.5 18 Fold:SAM domain-like Superfamily:5' to 3' exonuclease, C-terminal subdomain Family:5' to 3' exonuclease, C-terminal subdomain 47 c3rruA_ not modelled 6.5 11 PDB header:signaling protein Chain: A: PDB Molecule:tom1l1 protein; PDBTitle: x-ray crystal structure of the vhs domain of human tom1-like protein,2 northeast structural genomics consortium target hr3050e 48 c3nqwB_ not modelled 6.5 19 PDB header:hydrolase Chain: B: PDB Molecule:cg11900; PDBTitle: a metazoan ortholog of spot hydrolyzes ppgpp and plays a role in2 starvation responses 49 d1grja1 not modelled 6.2 14 Fold:Long alpha-hairpin Superfamily:GreA transcript cleavage protein, N-terminal domain Family:GreA transcript cleavage protein, N-terminal domain 50 c1miuA_ not modelled 6.1 23 PDB header:gene regulation/antitumor protein Chain: A: PDB Molecule:breast cancer type 2 susceptibility protein; PDBTitle: structure of a brca2-dss1 complex 51 c2uxsA_ not modelled 6.0 16 PDB header:hydrolase Chain: A: PDB Molecule:inorganic pyrophosphatase; PDBTitle: 2.7a crystal structure of inorganic pyrophosphatase (rv3628)2 from mycobacterium tuberculosis at ph 7.5 52 c1jfiA_ not modelled 5.8 14 PDB header:transcription/dna Chain: A: PDB Molecule:transcription regulator nc2 alpha chain; PDBTitle: crystal structure of the nc2-tbp-dna ternary complex 53 d1jfia_ not modelled 5.8 14 Fold:Histone-fold Superfamily:Histone-fold Family:TBP-associated factors, TAFs 54 d1h3ob_ not modelled 5.7 33 Fold:Histone-fold Superfamily:Histone-fold Family:TBP-associated factors, TAFs 55 c3m1eA_ not modelled 5.7 10 PDB header:transcription regulator Chain: A: PDB Molecule:hth-type transcriptional regulator benm; PDBTitle: crystal structure of benm_dbd 56 c2jobA_ not modelled 5.7 31 PDB header:lipid binding protein Chain: A: PDB Molecule:antilipopolysaccharide factor; PDBTitle: solution structure of an antilipopolysaccharide factor from2 shrimp and its possible lipid a binding site 57 d2pp4a1 not modelled 5.6 23 Fold:TAFH domain-like Superfamily:TAFH domain-like Family:TAFH domain-like 58 d1v5ua_ not modelled 5.6 18 Fold:PH domain-like barrel Superfamily:PH domain-like Family:Pleckstrin-homology domain (PH domain) 59 c2jveA_ not modelled 5.5 83 PDB header:toxin Chain: A: PDB Molecule:prod1; PDBTitle: solution structure of the extracellular domain of prod1, a2 protein implicated in proximodistal identity during3 amphibian limb regeneration 60 c2k9iB_ not modelled 5.3 21 PDB header:dna binding protein Chain: B: PDB Molecule:plasmid prn1, complete sequence; PDBTitle: nmr structure of plasmid copy control protein orf56 from2 sulfolobus islandicus 61 c1tt9B_ not modelled 5.3 24 PDB header:transferase, lyase Chain: B: PDB Molecule:formimidoyltransferase-cyclodeaminase PDBTitle: structure of the bifunctional and golgi associated2 formiminotransferase cyclodeaminase octamer 62 c1vs3B_ not modelled 5.2 21 PDB header:isomerase Chain: B: PDB Molecule:trna pseudouridine synthase a; PDBTitle: crystal structure of the trna pseudouridine synthase trua from thermus2 thermophilus hb8

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0