Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	T	I	W	E	I	S	E	K	A	D	Y	I	A	Q	R	H	R	R	L	Q	D	Q	W	H	I	Y	C	N	S	L	V	Q	G	I	T	L	S	K	A	R	L	H	H	A	M	S	C	A	P	D	K	E	L	C	F	V	L	F	E
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		H	F	R	I	Y	V	T	L	A	D	G	F	N	S	H	T	I	E	Y	Y	V	E	T	K	D	G	E	D	K	Q	R	I	A	Q	A	Q	L	S	I	D	G	M	I	D	G	K	V	N	I	R	D	R	E	Q	V	L	E	H	Y	L
Secondary structure
SS confidence
Disorder																							?	?	?	?	?	?	?	?	?				?					?				?								?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.
Sequence		E	K	I	A	G	V	Y	D	S	L	Y	T	A	I	E	N	N	V	P	V	N	L	S	Q	L	V	K	G	Q	S	P	A	A
Secondary structure
SS confidence
Disorder																											?	?	?	?	?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 2wpv chain G Region: 111 - 127 Aligned: 17 Modelled: 17 Confidence: 42.2% Identity: 29% PDB header:protein binding Chain: G: PDB Molecule:upf0363 protein yor164c; PDBTitle: crystal structure of s. cerevisiae get4-get5 complex Phyre2 PDB 3lpz chain A Region: 111 - 127 Aligned: 17 Modelled: 17 Confidence: 21.7% Identity: 29% PDB header:protein transport Chain: A: PDB Molecule:get4 (yor164c homolog); PDBTitle: crystal structure of c. therm. get4 Phyre2 PDB 2qgo chain A Region: 98 - 117 Aligned: 20 Modelled: 20 Confidence: 20.3% Identity: 35% PDB header:biosynthetic protein Chain: A: PDB Molecule:putative fe-s biosynthesis protein; PDBTitle: crystal structure of a putative fe-s biosynthesis protein from2 lactobacillus acidophilus Phyre2 PDB 3kcp chain A Region: 104 - 120 Aligned: 17 Modelled: 17 Confidence: 17.2% Identity: 29% PDB header:structural protein Chain: A: PDB Molecule:cellulosomal-scaffolding protein a; PDBTitle: crystal structure of interacting clostridium thermocellum2 multimodular components Phyre2 PDB 3iz5 chain F Region: 17 - 81 Aligned: 56 Modelled: 65 Confidence: 16.8% Identity: 16% PDB header:ribosome Chain: F: PDB Molecule:60s ribosomal protein l9 (l6p); PDBTitle: localization of the large subunit ribosomal proteins into a 5.5 a2 cryo-em map of triticum aestivum translating 80s ribosome Phyre2 PDB 2idb chain A domain 2 Region: 64 - 121 Aligned: 57 Modelled: 58 Confidence: 15.7% Identity: 18% Fold: UbiD C-terminal domain-like Superfamily: UbiD C-terminal domain-like Family: UbiD C-terminal domain-like Phyre2 PDB 1or7 chain C Region: 16 - 28 Aligned: 13 Modelled: 13 Confidence: 14.6% Identity: 23% Fold: N-terminal, cytoplasmic domain of anti-sigmaE factor RseA Superfamily: N-terminal, cytoplasmic domain of anti-sigmaE factor RseA Family: N-terminal, cytoplasmic domain of anti-sigmaE factor RseA Phyre2 PDB 1or7 chain C Region: 16 - 28 Aligned: 13 Modelled: 13 Confidence: 14.6% Identity: 23% PDB header:transcription Chain: C: PDB Molecule:sigma-e factor negative regulatory protein; PDBTitle: crystal structure of escherichia coli sigmae with the cytoplasmic2 domain of its anti-sigma rsea Phyre2 PDB 3bes chain L domain 1 Region: 119 - 143 Aligned: 25 Modelled: 25 Confidence: 13.6% Identity: 16% Fold: 4-helical cytokines Superfamily: 4-helical cytokines Family: Interferons/interleukin-10 (IL-10) Phyre2 PDB 3udi chain A Region: 72 - 123 Aligned: 18 Modelled: 18 Confidence: 13.1% Identity: 39% PDB header:penicillin-binding protein/antibiotic Chain: A: PDB Molecule:penicillin-binding protein 1a; PDBTitle: crystal structure of acinetobacter baumannii pbp1a in complex with2 penicillin g Phyre2 PDB 2e9h chain A Region: 93 - 122 Aligned: 30 Modelled: 30 Confidence: 12.1% Identity: 20% PDB header:translation Chain: A: PDB Molecule:eukaryotic translation initiation factor 5; PDBTitle: solution structure of the eif-5_eif-2b domain from human2 eukaryotic translation initiation factor 5 Phyre2 PDB 2e32 chain A Region: 41 - 73 Aligned: 32 Modelled: 33 Confidence: 11.0% Identity: 22% PDB header:ligase Chain: A: PDB Molecule:f-box only protein 2; PDBTitle: structural basis for selection of glycosylated substrate by2 scffbs1 ubiquitin ligase Phyre2 PDB 2dci chain A Region: 99 - 107 Aligned: 9 Modelled: 7 Confidence: 10.6% Identity: 56% PDB header:viral protein Chain: A: PDB Molecule:hemagglutinin; PDBTitle: nmr structure of influenza ha fusion peptide mutant w14a in2 dpc in ph5 Phyre2 PDB 2olv chain A Region: 112 - 123 Aligned: 12 Modelled: 12 Confidence: 9.9% Identity: 33% PDB header:transferase Chain: A: PDB Molecule:penicillin-binding protein 2; PDBTitle: structural insight into the transglycosylation step of bacterial cell2 wall biosynthesis : donor ligand complex Phyre2 PDB 3dwk chain C Region: 111 - 123 Aligned: 13 Modelled: 13 Confidence: 8.9% Identity: 31% PDB header:transferase Chain: C: PDB Molecule:penicillin-binding protein 2; PDBTitle: identification of dynamic structural motifs involved in2 peptidoglycan glycosyltransfer Phyre2 PDB 1xoo chain A Region: 100 - 107 Aligned: 8 Modelled: 6 Confidence: 8.7% Identity: 63% PDB header:viral protein Chain: A: PDB Molecule:hemagglutinin; PDBTitle: nmr structure of g1s mutant of influenza hemagglutinin2 fusion peptide in dpc micelles at ph 5 Phyre2 PDB 1xop chain A Region: 100 - 107 Aligned: 8 Modelled: 6 Confidence: 8.7% Identity: 63% PDB header:viral protein Chain: A: PDB Molecule:hemagglutinin; PDBTitle: nmr structure of g1v mutant of influenza hemagglutinin2 fusion peptide in dpc micelles at ph 5 Phyre2 PDB 3hlk chain B Region: 60 - 104 Aligned: 45 Modelled: 45 Confidence: 8.7% Identity: 22% PDB header:hydrolase Chain: B: PDB Molecule:acyl-coenzyme a thioesterase 2, mitochondrial; PDBTitle: crystal structure of human mitochondrial acyl-coa2 thioesterase (acot2) Phyre2 PDB 1eku chain A Region: 120 - 143 Aligned: 24 Modelled: 24 Confidence: 8.1% Identity: 17% PDB header:immune system Chain: A: PDB Molecule:interferon gamma; PDBTitle: crystal structure of a biologically active single chain2 mutant of human ifn-gamma Phyre2 PDB 2oq0 chain A domain 1 Region: 44 - 60 Aligned: 17 Modelled: 17 Confidence: 8.1% Identity: 24% Fold: OB-fold Superfamily: HIN-2000 domain-like Family: HIN-200/IF120x domain Phyre2 1 c2wpvG_ 2 c3lpzA_ 3 c2qgoA_ 4 c3kcpA_ 5 c3iz5F_ 6 d2idba2 7 d1or7c_ 8 c1or7C_ 9 d3besl1 10 c3udiA_ 11 c2e9hA_ 12 c2e32A_ 13 c2dciA_ 14 c2olvA_ 15 c3dwkC_ 16 c1xooA_ 17 c1xopA_ 18 c3hlkB_ 19 c1ekuA_ 20 d2oq0a1 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 c2wpvG_ 42.2 29 PDB header:protein binding Chain: G: PDB Molecule:upf0363 protein yor164c; PDBTitle: crystal structure of s. cerevisiae get4-get5 complex 2 c3lpzA_ 21.7 29 PDB header:protein transport Chain: A: PDB Molecule:get4 (yor164c homolog); PDBTitle: crystal structure of c. therm. get4 3 c2qgoA_ 20.3 35 PDB header:biosynthetic protein Chain: A: PDB Molecule:putative fe-s biosynthesis protein; PDBTitle: crystal structure of a putative fe-s biosynthesis protein from2 lactobacillus acidophilus 4 c3kcpA_ 17.2 29 PDB header:structural protein Chain: A: PDB Molecule:cellulosomal-scaffolding protein a; PDBTitle: crystal structure of interacting clostridium thermocellum2 multimodular components 5 c3iz5F_ 16.8 16 PDB header:ribosome Chain: F: PDB Molecule:60s ribosomal protein l9 (l6p); PDBTitle: localization of the large subunit ribosomal proteins into a 5.5 a2 cryo-em map of triticum aestivum translating 80s ribosome 6 d2idba2 15.7 18 Fold:UbiD C-terminal domain-like Superfamily:UbiD C-terminal domain-like Family:UbiD C-terminal domain-like 7 d1or7c_ 14.6 23 Fold:N-terminal, cytoplasmic domain of anti-sigmaE factor RseA Superfamily:N-terminal, cytoplasmic domain of anti-sigmaE factor RseA Family:N-terminal, cytoplasmic domain of anti-sigmaE factor RseA 8 c1or7C_ 14.6 23 PDB header:transcription Chain: C: PDB Molecule:sigma-e factor negative regulatory protein; PDBTitle: crystal structure of escherichia coli sigmae with the cytoplasmic2 domain of its anti-sigma rsea 9 d3besl1 13.6 16 Fold:4-helical cytokines Superfamily:4-helical cytokines Family:Interferons/interleukin-10 (IL-10) 10 c3udiA_ 13.1 39 PDB header:penicillin-binding protein/antibiotic Chain: A: PDB Molecule:penicillin-binding protein 1a; PDBTitle: crystal structure of acinetobacter baumannii pbp1a in complex with2 penicillin g 11 c2e9hA_ 12.1 20 PDB header:translation Chain: A: PDB Molecule:eukaryotic translation initiation factor 5; PDBTitle: solution structure of the eif-5_eif-2b domain from human2 eukaryotic translation initiation factor 5 12 c2e32A_ 11.0 22 PDB header:ligase Chain: A: PDB Molecule:f-box only protein 2; PDBTitle: structural basis for selection of glycosylated substrate by2 scffbs1 ubiquitin ligase 13 c2dciA_ 10.6 56 PDB header:viral protein Chain: A: PDB Molecule:hemagglutinin; PDBTitle: nmr structure of influenza ha fusion peptide mutant w14a in2 dpc in ph5 14 c2olvA_ 9.9 33 PDB header:transferase Chain: A: PDB Molecule:penicillin-binding protein 2; PDBTitle: structural insight into the transglycosylation step of bacterial cell2 wall biosynthesis : donor ligand complex 15 c3dwkC_ 8.9 31 PDB header:transferase Chain: C: PDB Molecule:penicillin-binding protein 2; PDBTitle: identification of dynamic structural motifs involved in2 peptidoglycan glycosyltransfer 16 c1xooA_ 8.7 63 PDB header:viral protein Chain: A: PDB Molecule:hemagglutinin; PDBTitle: nmr structure of g1s mutant of influenza hemagglutinin2 fusion peptide in dpc micelles at ph 5 17 c1xopA_ 8.7 63 PDB header:viral protein Chain: A: PDB Molecule:hemagglutinin; PDBTitle: nmr structure of g1v mutant of influenza hemagglutinin2 fusion peptide in dpc micelles at ph 5 18 c3hlkB_ 8.7 22 PDB header:hydrolase Chain: B: PDB Molecule:acyl-coenzyme a thioesterase 2, mitochondrial; PDBTitle: crystal structure of human mitochondrial acyl-coa2 thioesterase (acot2) 19 c1ekuA_ 8.1 17 PDB header:immune system Chain: A: PDB Molecule:interferon gamma; PDBTitle: crystal structure of a biologically active single chain2 mutant of human ifn-gamma 20 d2oq0a1 8.1 24 Fold:OB-fold Superfamily:HIN-2000 domain-like Family:HIN-200/IF120x domain 21 c2l4gA_ not modelled 8.0 63 PDB header:viral protein Chain: A: PDB Molecule:haemagglutinin; PDBTitle: influenza haemagglutinin fusion peptide mutant g13a 22 d1iyjb3 not modelled 8.0 30 Fold:OB-fold Superfamily:Nucleic acid-binding proteins Family:Single strand DNA-binding domain, SSB 23 c2jrdA_ not modelled 7.9 63 PDB header:viral protein Chain: A: PDB Molecule:hemagglutinin; PDBTitle: influenza hemagglutinin fusion domain mutant f9a 24 d1wi9a_ not modelled 7.5 24 Fold:DNA/RNA-binding 3-helical bundle Superfamily:"Winged helix" DNA-binding domain Family:PCI domain (PINT motif) 25 c2x49A_ not modelled 7.4 18 PDB header:protein transport Chain: A: PDB Molecule:invasion protein inva; PDBTitle: crystal structure of the c-terminal domain of inva 26 c2kk1A_ not modelled 7.1 26 PDB header:transferase Chain: A: PDB Molecule:tyrosine-protein kinase abl2; PDBTitle: solution structure of c-terminal domain of tyrosine-protein2 kinase abl2 from homo sapiens, northeast structural3 genomics consortium (nesg) target hr5537a 27 d2yrba1 not modelled 6.9 23 Fold:C2 domain-like Superfamily:C2 domain (Calcium/lipid-binding domain, CaLB) Family:PLC-like (P variant) 28 d2doda1 not modelled 6.8 32 Fold:Another 3-helical bundle Superfamily:FF domain Family:FF domain 29 d1e9ya1 not modelled 6.8 11 Fold:beta-clip Superfamily:Urease, beta-subunit Family:Urease, beta-subunit 30 c1iboA_ not modelled 6.6 63 PDB header:viral protein Chain: A: PDB Molecule:hemagglutinin ha2 chain peptide; PDBTitle: nmr structure of hemagglutinin fusion peptide in dpc2 micelles at ph 7.4 31 c1ibnA_ not modelled 6.6 63 PDB header:viral protein Chain: A: PDB Molecule:hemagglutinin ha2 chain peptide; PDBTitle: nmr structure of hemagglutinin fusion peptide in dpc2 micelles at ph 5 32 d1j5ya2 not modelled 6.6 50 Fold:HPr-like Superfamily:Putative transcriptional regulator TM1602, C-terminal domain Family:Putative transcriptional regulator TM1602, C-terminal domain 33 c3a5iB_ not modelled 6.2 41 PDB header:protein transport Chain: B: PDB Molecule:flagellar biosynthesis protein flha; PDBTitle: structure of the cytoplasmic domain of flha 34 c2oznB_ not modelled 6.1 26 PDB header:toxin Chain: B: PDB Molecule:hyalurononglucosaminidase; PDBTitle: the cohesin-dockerin complex of nagj and nagh from clostridium2 perfringens 35 c2bgcA_ not modelled 6.1 19 PDB header:transcription Chain: A: PDB Molecule:prfa; PDBTitle: prfa-g145s, a constitutive active mutant of the2 transcriptional regulator in l.monocytogenes 36 c3kz5E_ not modelled 5.9 33 PDB header:dna binding protein Chain: E: PDB Molecule:protein sopb; PDBTitle: structure of cdomain 37 c3mixA_ not modelled 5.8 29 PDB header:protein transport Chain: A: PDB Molecule:flagellar biosynthesis protein flha; PDBTitle: crystal structure of the cytosolic domain of b. subtilis flha 38 c3mydA_ not modelled 5.7 29 PDB header:protein transport Chain: A: PDB Molecule:flagellar biosynthesis protein flha; PDBTitle: structure of the cytoplasmic domain of flha from helicobacter pylori 39 d2oqoa1 not modelled 5.4 38 Fold:Lysozyme-like Superfamily:Lysozyme-like Family:PBP transglycosylase domain-like 40 d2f9yb1 not modelled 5.3 30 Fold:ClpP/crotonase Superfamily:ClpP/crotonase Family:Biotin dependent carboxylase carboxyltransferase domain 41 c2f9yB_ not modelled 5.3 30 PDB header:ligase Chain: B: PDB Molecule:acetyl-coenzyme a carboxylase carboxyl transferase subunit PDBTitle: the crystal structure of the carboxyltransferase subunit of acc from2 escherichia coli 42 c3p0dD_ not modelled 5.3 37 PDB header:hydrolase Chain: D: PDB Molecule:glycoside hydrolase family 9; PDBTitle: crystal structure of a multimodular ternary protein complex from2 clostridium thermocellum 43 c2zibA_ not modelled 5.3 29 PDB header:antifreeze protein Chain: A: PDB Molecule:type ii antifreeze protein; PDBTitle: crystal structure analysis of calcium-independent type ii2 antifreeze protein 44 d2olua1 not modelled 5.3 31 Fold:Lysozyme-like Superfamily:Lysozyme-like Family:PBP transglycosylase domain-like 45 d2cqna1 not modelled 5.2 4 Fold:Another 3-helical bundle Superfamily:FF domain Family:FF domain

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

---

Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0