Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	A	D	N	K	P	E	L	Q	R	G	L	E	A	R	H	I	E	L	I	A	L	G	G	T	I	G	V	G	L	F	M	G	A	A	S	T	L	K	W	A	G	P	S	V	L	L	A	Y	I	I	A	G	L	F	V	F	F	I	M
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		R	S	M	G	E	M	L	F	L	E	P	V	T	G	S	F	A	V	Y	A	H	R	Y	M	S	P	F	F	G	Y	L	T	A	W	S	Y	W	F	M	W	M	A	V	G	I	S	E	I	T	A	I	G	V	Y	V	Q	F	W	F	P
Secondary structure
SS confidence
Disorder									?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		E	M	A	Q	W	I	P	A	L	I	A	V	A	L	V	A	L	A	N	L	A	A	V	R	L	Y	G	E	I	E	F	W	F	A	M	I	K	V	T	T	I	I	V	M	I	V	I	G	L	G	V	I	F	F	G	F	G	N	G	G
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		Q	S	I	G	F	S	N	L	T	E	H	G	G	F	F	A	G	G	W	K	G	F	L	T	A	L	C	I	V	V	A	S	Y	Q	G	V	E	L	I	G	I	T	A	G	E	A	K	N	P	Q	V	T	L	R	S	A	V	G	K	V
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		L	W	R	I	L	I	F	Y	V	G	A	I	F	V	I	V	T	I	F	P	W	N	E	I	G	S	N	G	S	P	F	V	L	T	F	A	K	I	G	I	T	A	A	A	G	I	I	N	F	V	V	L	T	A	A	L	S	G	C	N
Secondary structure
SS confidence
Disorder																								?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		S	G	M	Y	S	C	G	R	M	L	Y	A	L	A	K	N	R	Q	L	P	A	A	M	A	K	V	S	R	H	G	V	P	V	A	G	V	A	V	S	I	A	I	L	L	I	G	S	C	L	N	Y	I	I	P	N	P	Q	R	V	F
Secondary structure
SS confidence
Disorder																																																			?		?		?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420
Sequence		V	Y	V	Y	S	A	S	V	L	P	G	M	V	P	W	F	V	I	L	I	S	Q	L	R	F	R	R	A	H	K	A	A	I	A	S	H	P	F	R	S	I	L	F	P	W	A	N	Y	V	T	M	A	F	L	I	C	V	L	I	G
Secondary structure
SS confidence
Disorder																															?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.	.	.	.	.	.	.	460	.
Sequence		M	Y	F	N	E	D	T	R	M	S	L	F	V	G	I	I	F	M	L	A	V	T	A	I	Y	K	V	F	G	L	N	R	H	G	K	A	H	K	L	E	E
Secondary structure
SS confidence
Disorder																																	?	?	?	?	?			?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 3gia chain A Region: 9 - 460 Aligned: 427 Modelled: 427 Confidence: 100.0% Identity: 15% PDB header:transport protein Chain: A: PDB Molecule:uncharacterized protein mj0609; PDBTitle: crystal structure of apct transporter Phyre2 PDB 3lrc chain C Region: 9 - 456 Aligned: 408 Modelled: 408 Confidence: 100.0% Identity: 21% PDB header:transport protein Chain: C: PDB Molecule:arginine/agmatine antiporter; PDBTitle: structure of e. coli adic (p1) Phyre2 PDB 2jln chain A Region: 3 - 461 Aligned: 441 Modelled: 441 Confidence: 100.0% Identity: 9% PDB header:membrane protein Chain: A: PDB Molecule:mhp1; PDBTitle: structure of mhp1, a nucleobase-cation-symport-1 family2 transporter Phyre2 PDB 2xq2 chain A Region: 10 - 461 Aligned: 432 Modelled: 437 Confidence: 99.6% Identity: 10% PDB header:transport protein Chain: A: PDB Molecule:sodium/glucose cotransporter; PDBTitle: structure of the k294a mutant of vsglt Phyre2 PDB 3dh4 chain A Region: 10 - 461 Aligned: 435 Modelled: 435 Confidence: 99.5% Identity: 10% PDB header:transport protein Chain: A: PDB Molecule:sodium/glucose cotransporter; PDBTitle: crystal structure of sodium/sugar symporter with bound galactose from2 vibrio parahaemolyticus Phyre2 PDB 2a65 chain A domain 1 Region: 13 - 457 Aligned: 435 Modelled: 435 Confidence: 98.6% Identity: 14% Fold: SNF-like Superfamily: SNF-like Family: SNF-like Phyre2 PDB 2w8a chain C Region: 5 - 401 Aligned: 384 Modelled: 386 Confidence: 98.1% Identity: 12% PDB header:membrane protein Chain: C: PDB Molecule:glycine betaine transporter betp; PDBTitle: crystal structure of the sodium-coupled glycine betaine2 symporter betp from corynebacterium glutamicum with bound3 substrate Phyre2 PDB 3hfx chain A Region: 46 - 392 Aligned: 337 Modelled: 339 Confidence: 96.1% Identity: 8% PDB header:transport protein Chain: A: PDB Molecule:l-carnitine/gamma-butyrobetaine antiporter; PDBTitle: crystal structure of carnitine transporter Phyre2 PDB 1fft chain B domain 2 Region: 357 - 422 Aligned: 66 Modelled: 66 Confidence: 56.0% Identity: 12% Fold: Transmembrane helix hairpin Superfamily: Cytochrome c oxidase subunit II-like, transmembrane region Family: Cytochrome c oxidase subunit II-like, transmembrane region Phyre2 PDB 2l1n chain A Region: 2 - 14 Aligned: 13 Modelled: 13 Confidence: 17.7% Identity: 31% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:uncharacterized protein; PDBTitle: solution nmr structure of the protein yp_399305.1 Phyre2 PDB 2knc chain A Region: 429 - 461 Aligned: 33 Modelled: 33 Confidence: 17.2% Identity: 9% PDB header:cell adhesion Chain: A: PDB Molecule:integrin alpha-iib; PDBTitle: platelet integrin alfaiib-beta3 transmembrane-cytoplasmic2 heterocomplex Phyre2 PDB 1fft chain G Region: 357 - 423 Aligned: 67 Modelled: 67 Confidence: 14.9% Identity: 12% PDB header:oxidoreductase Chain: G: PDB Molecule:ubiquinol oxidase; PDBTitle: the structure of ubiquinol oxidase from escherichia coli Phyre2 PDB 3qnq chain D Region: 416 - 461 Aligned: 46 Modelled: 46 Confidence: 13.2% Identity: 11% PDB header:membrane protein, transport protein Chain: D: PDB Molecule:pts system, cellobiose-specific iic component; PDBTitle: crystal structure of the transporter chbc, the iic component from the2 n,n'-diacetylchitobiose-specific phosphotransferase system Phyre2 PDB 1m57 chain H Region: 357 - 422 Aligned: 66 Modelled: 66 Confidence: 12.0% Identity: 14% PDB header:oxidoreductase Chain: H: PDB Molecule:cytochrome c oxidase; PDBTitle: structure of cytochrome c oxidase from rhodobacter2 sphaeroides (eq(i-286) mutant)) Phyre2 PDB 1zxa chain B Region: 3 - 12 Aligned: 10 Modelled: 10 Confidence: 8.7% Identity: 40% PDB header:transferase Chain: B: PDB Molecule:cgmp-dependent protein kinase 1, alpha isozyme; PDBTitle: solution structure of the coiled-coil domain of cgmp-2 dependent protein kinase ia Phyre2 PDB 2rdd chain B Region: 435 - 461 Aligned: 27 Modelled: 27 Confidence: 7.7% Identity: 4% PDB header:membrane protein/transport protein Chain: B: PDB Molecule:upf0092 membrane protein yajc; PDBTitle: x-ray crystal structure of acrb in complex with a novel2 transmembrane helix. Phyre2 PDB 1qle chain B Region: 357 - 422 Aligned: 66 Modelled: 66 Confidence: 7.6% Identity: 15% PDB header:oxidoreductase/immune system Chain: B: PDB Molecule:cytochrome c oxidase polypeptide ii; PDBTitle: cryo-structure of the paracoccus denitrificans four-subunit2 cytochrome c oxidase in the completely oxidized state3 complexed with an antibody fv fragment Phyre2 PDB 1ar1 chain B Region: 357 - 422 Aligned: 66 Modelled: 66 Confidence: 7.6% Identity: 15% PDB header:complex (oxidoreductase/antibody) Chain: B: PDB Molecule:cytochrome c oxidase; PDBTitle: structure at 2.7 angstrom resolution of the paracoccus2 denitrificans two-subunit cytochrome c oxidase complexed3 with an antibody fv fragment Phyre2 PDB 2bbj chain B Region: 405 - 454 Aligned: 50 Modelled: 50 Confidence: 7.5% Identity: 10% PDB header:metal transport/membrane protein Chain: B: PDB Molecule:divalent cation transport-related protein; PDBTitle: crystal structure of the cora mg2+ transporter Phyre2 PDB 3rko chain F Region: 329 - 457 Aligned: 126 Modelled: 129 Confidence: 7.3% Identity: 10% PDB header:oxidoreductase Chain: F: PDB Molecule:nadh-quinone oxidoreductase subunit j; PDBTitle: crystal structure of the membrane domain of respiratory complex i from2 e. coli at 3.0 angstrom resolution Phyre2 1 c3giaA_ 2 c3lrcC_ 3 c2jlnA_ 4 c2xq2A_ 5 c3dh4A_ 6 d2a65a1 7 c2w8aC_ 8 c3hfxA_ 9 d1fftb2 10 c2l1nA_ 11 c2kncA_ 12 c1fftG_ 13 c3qnqD_ 14 c1m57H_ 15 c1zxaB_ 16 c2rddB_ 17 c1qleB_ 18 c1ar1B_ 19 c2bbjB_ 20 c3rkoF_ 21 22 23 24 25

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 c3giaA_ 100.0 15 PDB header:transport protein Chain: A: PDB Molecule:uncharacterized protein mj0609; PDBTitle: crystal structure of apct transporter 2 c3lrcC_ 100.0 21 PDB header:transport protein Chain: C: PDB Molecule:arginine/agmatine antiporter; PDBTitle: structure of e. coli adic (p1) 3 c2jlnA_ 100.0 9 PDB header:membrane protein Chain: A: PDB Molecule:mhp1; PDBTitle: structure of mhp1, a nucleobase-cation-symport-1 family2 transporter 4 c2xq2A_ 99.6 10 PDB header:transport protein Chain: A: PDB Molecule:sodium/glucose cotransporter; PDBTitle: structure of the k294a mutant of vsglt 5 c3dh4A_ 99.5 10 PDB header:transport protein Chain: A: PDB Molecule:sodium/glucose cotransporter; PDBTitle: crystal structure of sodium/sugar symporter with bound galactose from2 vibrio parahaemolyticus 6 d2a65a1 98.6 14 Fold:SNF-like Superfamily:SNF-like Family:SNF-like 7 c2w8aC_ 98.1 12 PDB header:membrane protein Chain: C: PDB Molecule:glycine betaine transporter betp; PDBTitle: crystal structure of the sodium-coupled glycine betaine2 symporter betp from corynebacterium glutamicum with bound3 substrate 8 c3hfxA_ 96.1 8 PDB header:transport protein Chain: A: PDB Molecule:l-carnitine/gamma-butyrobetaine antiporter; PDBTitle: crystal structure of carnitine transporter 9 d1fftb2 56.0 12 Fold:Transmembrane helix hairpin Superfamily:Cytochrome c oxidase subunit II-like, transmembrane region Family:Cytochrome c oxidase subunit II-like, transmembrane region 10 c2l1nA_ 17.7 31 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:uncharacterized protein; PDBTitle: solution nmr structure of the protein yp_399305.1 11 c2kncA_ 17.2 9 PDB header:cell adhesion Chain: A: PDB Molecule:integrin alpha-iib; PDBTitle: platelet integrin alfaiib-beta3 transmembrane-cytoplasmic2 heterocomplex 12 c1fftG_ 14.9 12 PDB header:oxidoreductase Chain: G: PDB Molecule:ubiquinol oxidase; PDBTitle: the structure of ubiquinol oxidase from escherichia coli 13 c3qnqD_ 13.2 11 PDB header:membrane protein, transport protein Chain: D: PDB Molecule:pts system, cellobiose-specific iic component; PDBTitle: crystal structure of the transporter chbc, the iic component from the2 n,n'-diacetylchitobiose-specific phosphotransferase system 14 c1m57H_ 12.0 14 PDB header:oxidoreductase Chain: H: PDB Molecule:cytochrome c oxidase; PDBTitle: structure of cytochrome c oxidase from rhodobacter2 sphaeroides (eq(i-286) mutant)) 15 c1zxaB_ 8.7 40 PDB header:transferase Chain: B: PDB Molecule:cgmp-dependent protein kinase 1, alpha isozyme; PDBTitle: solution structure of the coiled-coil domain of cgmp-2 dependent protein kinase ia 16 c2rddB_ 7.7 4 PDB header:membrane protein/transport protein Chain: B: PDB Molecule:upf0092 membrane protein yajc; PDBTitle: x-ray crystal structure of acrb in complex with a novel2 transmembrane helix. 17 c1qleB_ 7.6 15 PDB header:oxidoreductase/immune system Chain: B: PDB Molecule:cytochrome c oxidase polypeptide ii; PDBTitle: cryo-structure of the paracoccus denitrificans four-subunit2 cytochrome c oxidase in the completely oxidized state3 complexed with an antibody fv fragment 18 c1ar1B_ 7.6 15 PDB header:complex (oxidoreductase/antibody) Chain: B: PDB Molecule:cytochrome c oxidase; PDBTitle: structure at 2.7 angstrom resolution of the paracoccus2 denitrificans two-subunit cytochrome c oxidase complexed3 with an antibody fv fragment 19 c2bbjB_ 7.5 10 PDB header:metal transport/membrane protein Chain: B: PDB Molecule:divalent cation transport-related protein; PDBTitle: crystal structure of the cora mg2+ transporter 20 c3rkoF_ 7.3 10 PDB header:oxidoreductase Chain: F: PDB Molecule:nadh-quinone oxidoreductase subunit j; PDBTitle: crystal structure of the membrane domain of respiratory complex i from2 e. coli at 3.0 angstrom resolution 21 c3b8eC_ not modelled 7.0 11 PDB header:hydrolase/transport protein Chain: C: PDB Molecule:sodium/potassium-transporting atpase subunit PDBTitle: crystal structure of the sodium-potassium pump 22 d1iwga8 not modelled 6.9 12 Fold:Multidrug efflux transporter AcrB transmembrane domain Superfamily:Multidrug efflux transporter AcrB transmembrane domain Family:Multidrug efflux transporter AcrB transmembrane domain 23 c3ixzA_ not modelled 6.5 11 PDB header:hydrolase Chain: A: PDB Molecule:potassium-transporting atpase alpha; PDBTitle: pig gastric h+/k+-atpase complexed with aluminium fluoride 24 d3ehbb2 not modelled 6.0 15 Fold:Transmembrane helix hairpin Superfamily:Cytochrome c oxidase subunit II-like, transmembrane region Family:Cytochrome c oxidase subunit II-like, transmembrane region 25 d3dtub2 not modelled 5.2 14 Fold:Transmembrane helix hairpin Superfamily:Cytochrome c oxidase subunit II-like, transmembrane region Family:Cytochrome c oxidase subunit II-like, transmembrane region

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

Transmembrane helix prediction

Transmembrane helices have been predicted in your sequence to adopt the topology shown below

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0