Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	F	T	D	T	I	N	K	C	A	A	N	A	A	R	I	A	R	L	S	A	N	N	P	L	G	F	W	V	S	S	A	M	A	G	A	Y	V	G	L	G	I	I	L	I	F	T	L	G	N	L	L	D	P	S	V	R	P	L	V
Secondary structure
SS confidence
Disorder		?	?	?															?	?	?	?																														?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		M	G	A	T	F	G	I	A	L	T	L	V	I	I	A	G	S	E	L	F	T	G	H	T	M	F	L	T	F	G	V	K	A	G	S	I	S	H	G	Q	M	W	A	I	L	P	Q	T	W	L	G	N	L	V	G	S	V	F	V	A
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		M	L	Y	S	W	G	G	G	S	L	L	P	V	D	T	S	I	V	H	S	V	A	L	A	K	T	T	A	P	A	M	V	L	F	F	K	G	A	L	C	N	W	L	V	C	L	A	I	W	M	A	L	R	T	E	G	A	A	K	F
Secondary structure
SS confidence
Disorder									?	?																																										?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		I	A	I	W	W	C	L	L	A	F	I	A	S	G	Y	E	H	S	I	A	N	M	T	L	F	A	L	S	W	F	G	N	H	S	E	A	Y	T	L	A	G	I	G	H	N	L	L	W	V	T	L	G	N	T	L	S	G	A	V	F
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.
Sequence		M	G	L	G	Y	W	Y	A	T	P	K	A	N	R	P	V	A	D	K	F	N	Q	T	E	T	A	A	G
Secondary structure
SS confidence
Disorder												?	?	?	?	?	?	?			?	?	?	?	?	?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 3klz chain E Region: 14 - 251 Aligned: 234 Modelled: 238 Confidence: 100.0% Identity: 27% PDB header:membrane protein Chain: E: PDB Molecule:putative formate transporter 1; PDBTitle: pentameric formate channel with formate bound Phyre2 PDB 3kcv chain G Region: 22 - 251 Aligned: 228 Modelled: 230 Confidence: 100.0% Identity: 28% PDB header:transport protein Chain: G: PDB Molecule:probable formate transporter 1; PDBTitle: structure of formate channel Phyre2 PDB 3llq chain B Region: 19 - 251 Aligned: 229 Modelled: 233 Confidence: 95.5% Identity: 9% PDB header:membrane protein Chain: B: PDB Molecule:aquaporin z 2; PDBTitle: aquaporin structure from plant pathogen agrobacterium tumerfaciens Phyre2 PDB 1lda chain A Region: 23 - 252 Aligned: 229 Modelled: 230 Confidence: 93.7% Identity: 10% PDB header:transport protein Chain: A: PDB Molecule:glycerol uptake facilitator protein; PDBTitle: crystal structure of the e. coli glycerol facilitator (glpf) without2 substrate glycerol Phyre2 PDB 3d9s chain B Region: 16 - 258 Aligned: 227 Modelled: 243 Confidence: 93.1% Identity: 9% PDB header:membrane protein Chain: B: PDB Molecule:aquaporin-5; PDBTitle: human aquaporin 5 (aqp5) - high resolution x-ray structure Phyre2 PDB 1j4n chain A Region: 17 - 261 Aligned: 228 Modelled: 245 Confidence: 91.2% Identity: 11% Fold: Aquaporin-like Superfamily: Aquaporin-like Family: Aquaporin-like Phyre2 PDB 1fx8 chain A Region: 23 - 252 Aligned: 229 Modelled: 230 Confidence: 88.9% Identity: 10% Fold: Aquaporin-like Superfamily: Aquaporin-like Family: Aquaporin-like Phyre2 PDB 1rc2 chain A Region: 26 - 253 Aligned: 221 Modelled: 228 Confidence: 87.5% Identity: 12% Fold: Aquaporin-like Superfamily: Aquaporin-like Family: Aquaporin-like Phyre2 PDB 1ymg chain A Region: 22 - 261 Aligned: 220 Modelled: 240 Confidence: 78.5% Identity: 12% PDB header:membrane protein Chain: A: PDB Molecule:lens fiber major intrinsic protein; PDBTitle: the channel architecture of aquaporin o at 2.2 angstrom resolution Phyre2 PDB 1ymg chain A domain 1 Region: 22 - 261 Aligned: 220 Modelled: 240 Confidence: 78.5% Identity: 12% Fold: Aquaporin-like Superfamily: Aquaporin-like Family: Aquaporin-like Phyre2 PDB 2knc chain A Region: 227 - 264 Aligned: 38 Modelled: 38 Confidence: 73.0% Identity: 21% PDB header:cell adhesion Chain: A: PDB Molecule:integrin alpha-iib; PDBTitle: platelet integrin alfaiib-beta3 transmembrane-cytoplasmic2 heterocomplex Phyre2 PDB 3c02 chain A Region: 23 - 260 Aligned: 227 Modelled: 238 Confidence: 62.1% Identity: 11% PDB header:membrane protein Chain: A: PDB Molecule:aquaglyceroporin; PDBTitle: x-ray structure of the aquaglyceroporin from plasmodium falciparum Phyre2 PDB 2b5f chain D Region: 18 - 248 Aligned: 218 Modelled: 231 Confidence: 36.3% Identity: 10% PDB header:transport protein,membrane protein Chain: D: PDB Molecule:aquaporin; PDBTitle: crystal structure of the spinach aquaporin sopip2;1 in an2 open conformation to 3.9 resolution Phyre2 PDB 2w2e chain A Region: 22 - 195 Aligned: 163 Modelled: 174 Confidence: 35.9% Identity: 9% PDB header:membrane protein Chain: A: PDB Molecule:aquaporin; PDBTitle: 1.15 angstrom crystal structure of p.pastoris aquaporin,2 aqy1, in a closed conformation at ph 3.5 Phyre2 PDB 3gd8 chain A Region: 22 - 250 Aligned: 212 Modelled: 229 Confidence: 34.3% Identity: 14% PDB header:membrane protein Chain: A: PDB Molecule:aquaporin-4; PDBTitle: crystal structure of human aquaporin 4 at 1.8 and its mechanism of2 conductance Phyre2 PDB 3iyz chain A Region: 21 - 249 Aligned: 212 Modelled: 229 Confidence: 29.1% Identity: 15% PDB header:transport protein Chain: A: PDB Molecule:aquaporin-4; PDBTitle: structure of aquaporin-4 s180d mutant at 10.0 a resolution from2 electron micrograph Phyre2 PDB 1pv7 chain A Region: 234 - 267 Aligned: 34 Modelled: 34 Confidence: 16.5% Identity: 15% Fold: MFS general substrate transporter Superfamily: MFS general substrate transporter Family: LacY-like proton/sugar symporter Phyre2 PDB 1c17 chain M Region: 230 - 247 Aligned: 18 Modelled: 18 Confidence: 15.9% Identity: 22% Fold: F1F0 ATP synthase subunit A Superfamily: F1F0 ATP synthase subunit A Family: F1F0 ATP synthase subunit A Phyre2 PDB 2nr9 chain A domain 1 Region: 237 - 258 Aligned: 22 Modelled: 22 Confidence: 11.4% Identity: 14% Fold: Rhomboid-like Superfamily: Rhomboid-like Family: Rhomboid-like Phyre2 PDB 2rdd chain B Region: 238 - 258 Aligned: 21 Modelled: 21 Confidence: 9.8% Identity: 14% PDB header:membrane protein/transport protein Chain: B: PDB Molecule:upf0092 membrane protein yajc; PDBTitle: x-ray crystal structure of acrb in complex with a novel2 transmembrane helix. Phyre2 1 c3klzE_ 2 c3kcvG_ 3 c3llqB_ 4 c1ldaA_ 5 c3d9sB_ 6 d1j4na_ 7 d1fx8a_ 8 d1rc2a_ 9 c1ymgA_ 10 d1ymga1 11 c2kncA_ 12 c3c02A_ 13 c2b5fD_ 14 c2w2eA_ 15 c3gd8A_ 16 c3iyzA_ 17 d1pv7a_ 18 d1c17m_ 19 d2nr9a1 20 c2rddB_ 21 22 23 24 25 26 27 28

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 c3klzE_ 100.0 27 PDB header:membrane protein Chain: E: PDB Molecule:putative formate transporter 1; PDBTitle: pentameric formate channel with formate bound 2 c3kcvG_ 100.0 28 PDB header:transport protein Chain: G: PDB Molecule:probable formate transporter 1; PDBTitle: structure of formate channel 3 c3llqB_ 95.5 9 PDB header:membrane protein Chain: B: PDB Molecule:aquaporin z 2; PDBTitle: aquaporin structure from plant pathogen agrobacterium tumerfaciens 4 c1ldaA_ 93.7 10 PDB header:transport protein Chain: A: PDB Molecule:glycerol uptake facilitator protein; PDBTitle: crystal structure of the e. coli glycerol facilitator (glpf) without2 substrate glycerol 5 c3d9sB_ 93.1 9 PDB header:membrane protein Chain: B: PDB Molecule:aquaporin-5; PDBTitle: human aquaporin 5 (aqp5) - high resolution x-ray structure 6 d1j4na_ 91.2 11 Fold:Aquaporin-like Superfamily:Aquaporin-like Family:Aquaporin-like 7 d1fx8a_ 88.9 10 Fold:Aquaporin-like Superfamily:Aquaporin-like Family:Aquaporin-like 8 d1rc2a_ 87.5 12 Fold:Aquaporin-like Superfamily:Aquaporin-like Family:Aquaporin-like 9 c1ymgA_ 78.5 12 PDB header:membrane protein Chain: A: PDB Molecule:lens fiber major intrinsic protein; PDBTitle: the channel architecture of aquaporin o at 2.2 angstrom resolution 10 d1ymga1 78.5 12 Fold:Aquaporin-like Superfamily:Aquaporin-like Family:Aquaporin-like 11 c2kncA_ 73.0 21 PDB header:cell adhesion Chain: A: PDB Molecule:integrin alpha-iib; PDBTitle: platelet integrin alfaiib-beta3 transmembrane-cytoplasmic2 heterocomplex 12 c3c02A_ 62.1 11 PDB header:membrane protein Chain: A: PDB Molecule:aquaglyceroporin; PDBTitle: x-ray structure of the aquaglyceroporin from plasmodium falciparum 13 c2b5fD_ 36.3 10 PDB header:transport protein,membrane protein Chain: D: PDB Molecule:aquaporin; PDBTitle: crystal structure of the spinach aquaporin sopip2;1 in an2 open conformation to 3.9 resolution 14 c2w2eA_ 35.9 9 PDB header:membrane protein Chain: A: PDB Molecule:aquaporin; PDBTitle: 1.15 angstrom crystal structure of p.pastoris aquaporin,2 aqy1, in a closed conformation at ph 3.5 15 c3gd8A_ 34.3 14 PDB header:membrane protein Chain: A: PDB Molecule:aquaporin-4; PDBTitle: crystal structure of human aquaporin 4 at 1.8 and its mechanism of2 conductance 16 c3iyzA_ 29.1 15 PDB header:transport protein Chain: A: PDB Molecule:aquaporin-4; PDBTitle: structure of aquaporin-4 s180d mutant at 10.0 a resolution from2 electron micrograph 17 d1pv7a_ 16.5 15 Fold:MFS general substrate transporter Superfamily:MFS general substrate transporter Family:LacY-like proton/sugar symporter 18 d1c17m_ 15.9 22 Fold:F1F0 ATP synthase subunit A Superfamily:F1F0 ATP synthase subunit A Family:F1F0 ATP synthase subunit A 19 d2nr9a1 11.4 14 Fold:Rhomboid-like Superfamily:Rhomboid-like Family:Rhomboid-like 20 c2rddB_ 9.8 14 PDB header:membrane protein/transport protein Chain: B: PDB Molecule:upf0092 membrane protein yajc; PDBTitle: x-ray crystal structure of acrb in complex with a novel2 transmembrane helix. 21 c2jy0A_ not modelled 9.7 39 PDB header:membrane protein, viral protein Chain: A: PDB Molecule:protease ns2-3; PDBTitle: solution nmr structure of hcv ns2 protein, membrane segment2 (1-27) 22 c2f2bA_ not modelled 7.5 13 PDB header:membrane protein Chain: A: PDB Molecule:aquaporin aqpm; PDBTitle: crystal structure of integral membrane protein aquaporin aqpm at 1.68a2 resolution 23 c3qnqD_ not modelled 7.4 9 PDB header:membrane protein, transport protein Chain: D: PDB Molecule:pts system, cellobiose-specific iic component; PDBTitle: crystal structure of the transporter chbc, the iic component from the2 n,n'-diacetylchitobiose-specific phosphotransferase system 24 d2foka2 not modelled 6.2 30 Fold:DNA/RNA-binding 3-helical bundle Superfamily:"Winged helix" DNA-binding domain Family:Restriction endonuclease FokI, N-terminal (recognition) domain 25 d2c1wa1 not modelled 6.1 50 Fold:EndoU-like Superfamily:EndoU-like Family:Eukaryotic EndoU ribonuclease 26 c2jp3A_ not modelled 6.1 14 PDB header:transcription Chain: A: PDB Molecule:fxyd domain-containing ion transport regulator 4; PDBTitle: solution structure of the human fxyd4 (chif) protein in sds2 micelles 27 c2vv5D_ not modelled 5.9 12 PDB header:membrane protein Chain: D: PDB Molecule:small-conductance mechanosensitive channel; PDBTitle: the open structure of mscs 28 c2voyG_ not modelled 5.9 50 PDB header:hydrolase Chain: G: PDB Molecule:sarcoplasmic/endoplasmic reticulum calcium PDBTitle: cryoem model of copa, the copper transporting atpase from2 archaeoglobus fulgidus

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

Transmembrane helix prediction

Transmembrane helices have been predicted in your sequence to adopt the topology shown below

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0