Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	K	Y	Q	Q	L	E	N	L	E	S	G	W	K	W	K	Y	L	V	K	K	H	R	E	G	E	L	I	T	R	Y	I	E	A	S	A	A	Q	E	A	V	D	V	L	L	S	L	E	N	E	P	V	L	V	N	G	W	I	D	K
Secondary structure
SS confidence
Disorder		?	?				?	?	?	?																																								?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		H	M	N	P	E	L	V	N	R	M	K	Q	T	I	R	A	R	R	K	R	H	F	N	A	E	H	Q	H	T	R	K	K	S	I	D	L	E	F	I	V	W	Q	R	L	A	G	L	A	Q	R	R	G	K	T	L	S	E	T	I	V
Secondary structure
SS confidence
Disorder																						?	?	?				?	?	?	?	?	?																			?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150
Sequence		Q	L	I	E	D	A	E	N	K	E	K	Y	A	N	K	M	S	S	L	K	Q	D	L	Q	A	L	L	G	K	E
Secondary structure
SS confidence
Disorder								?	?																			?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 3kk4 chain B Region: 93 - 126 Aligned: 34 Modelled: 34 Confidence: 87.0% Identity: 29% PDB header:structural genomics, unknown function Chain: B: PDB Molecule:uncharacterized protein bp1543; PDBTitle: uncharacterized protein bp1543 from bordetella pertussis tohama i Phyre2 PDB 2ay0 chain A domain 1 Region: 89 - 125 Aligned: 37 Modelled: 37 Confidence: 32.6% Identity: 24% Fold: Ribbon-helix-helix Superfamily: Ribbon-helix-helix Family: PutA pre-N-terminal region-like Phyre2 PDB 2aua chain A domain 1 Region: 64 - 78 Aligned: 15 Modelled: 15 Confidence: 22.7% Identity: 40% Fold: ADP-ribosylation Superfamily: ADP-ribosylation Family: BC2332-like Phyre2 PDB 3nsn chain A Region: 95 - 110 Aligned: 16 Modelled: 16 Confidence: 21.0% Identity: 44% PDB header:hydrolase Chain: A: PDB Molecule:n-acetylglucosaminidase; PDBTitle: crystal structure of insect beta-n-acetyl-d-hexosaminidase ofhex12 complexed with tmg-chitotriomycin Phyre2 PDB 1ug3 chain A domain 2 Region: 38 - 76 Aligned: 38 Modelled: 39 Confidence: 20.2% Identity: 18% Fold: alpha-alpha superhelix Superfamily: ARM repeat Family: MIF4G domain-like Phyre2 PDB 1qba chain A domain 3 Region: 95 - 111 Aligned: 17 Modelled: 17 Confidence: 14.4% Identity: 24% Fold: TIM beta/alpha-barrel Superfamily: (Trans)glycosidases Family: beta-N-acetylhexosaminidase catalytic domain Phyre2 PDB 1qba chain A Region: 95 - 110 Aligned: 16 Modelled: 16 Confidence: 13.8% Identity: 25% PDB header:glycosyl hydrolase Chain: A: PDB Molecule:chitobiase; PDBTitle: bacterial chitobiase, glycosyl hydrolase family 20 Phyre2 PDB 2pnv chain A Region: 122 - 148 Aligned: 27 Modelled: 27 Confidence: 12.3% Identity: 22% PDB header:membrane protein Chain: A: PDB Molecule:small conductance calcium-activated potassium PDBTitle: crystal structure of the leucine zipper domain of small-2 conductance ca2+-activated k+ (skca) channel from rattus3 norvegicus Phyre2 PDB 3rcn chain A Region: 95 - 110 Aligned: 16 Modelled: 16 Confidence: 12.2% Identity: 19% PDB header:hydrolase Chain: A: PDB Molecule:beta-n-acetylhexosaminidase; PDBTitle: crystal structure of beta-n-acetylhexosaminidase from arthrobacter2 aurescens Phyre2 PDB 2qzc chain B Region: 38 - 101 Aligned: 62 Modelled: 64 Confidence: 11.5% Identity: 16% PDB header:lyase Chain: B: PDB Molecule:transcriptional activator tena-1; PDBTitle: crystal structure of a putative tena-like thiaminase (tena-1, sso2206)2 from sulfolobus solfataricus p2 at 1.50 a resolution Phyre2 PDB 2rbf chain B Region: 93 - 124 Aligned: 32 Modelled: 32 Confidence: 10.7% Identity: 25% PDB header:oxidoreductase/dna Chain: B: PDB Molecule:bifunctional protein puta; PDBTitle: structure of the ribbon-helix-helix domain of escherichia coli puta2 (puta52) complexed with operator dna (o2) Phyre2 PDB 1m04 chain A Region: 95 - 111 Aligned: 17 Modelled: 17 Confidence: 10.6% Identity: 29% PDB header:hydrolase Chain: A: PDB Molecule:beta-n-acetylhexosaminidase; PDBTitle: mutant streptomyces plicatus beta-hexosaminidase (d313n) in complex2 with product (glcnac) Phyre2 PDB 2ahm chain A domain 1 Region: 8 - 24 Aligned: 17 Modelled: 17 Confidence: 10.5% Identity: 35% Fold: immunoglobulin/albumin-binding domain-like Superfamily: Coronavirus NSP7-like Family: Coronavirus NSP7-like Phyre2 PDB 1ysy chain A domain 1 Region: 8 - 24 Aligned: 17 Modelled: 17 Confidence: 10.2% Identity: 35% Fold: immunoglobulin/albumin-binding domain-like Superfamily: Coronavirus NSP7-like Family: Coronavirus NSP7-like Phyre2 PDB 1jak chain A domain 1 Region: 95 - 110 Aligned: 16 Modelled: 16 Confidence: 9.4% Identity: 31% Fold: TIM beta/alpha-barrel Superfamily: (Trans)glycosidases Family: beta-N-acetylhexosaminidase catalytic domain Phyre2 PDB 2o59 chain B Region: 23 - 78 Aligned: 56 Modelled: 56 Confidence: 8.4% Identity: 23% PDB header:isomerase/dna Chain: B: PDB Molecule:dna topoisomerase 3; PDBTitle: structure of e. coli topoisomerase iii in complex with an 8-2 base single stranded oligonucleotide. frozen in glycerol3 ph 8.0 Phyre2 PDB 2p62 chain A domain 1 Region: 112 - 143 Aligned: 32 Modelled: 32 Confidence: 8.3% Identity: 31% Fold: PH0156-like Superfamily: PH0156-like Family: PH0156-like Phyre2 PDB 2p58 chain A Region: 98 - 106 Aligned: 9 Modelled: 9 Confidence: 7.9% Identity: 44% PDB header:transport protein/chaperone Chain: A: PDB Molecule:putative type iii secretion protein ysce; PDBTitle: structure of the yersinia pestis type iii secretion system2 needle protein yscf in complex with its chaperones3 ysce/yscg Phyre2 PDB 1i7d chain A Region: 23 - 78 Aligned: 56 Modelled: 56 Confidence: 7.9% Identity: 23% Fold: Prokaryotic type I DNA topoisomerase Superfamily: Prokaryotic type I DNA topoisomerase Family: Prokaryotic type I DNA topoisomerase Phyre2 PDB 1v4a chain A domain 1 Region: 69 - 101 Aligned: 33 Modelled: 31 Confidence: 7.5% Identity: 21% Fold: Four-helical up-and-down bundle Superfamily: Nucleotidyltransferase substrate binding subunit/domain Family: Glutamine synthase adenylyltransferase GlnE, domain 2 Phyre2 1 c3kk4B_ 2 d2ay0a1 3 d2auaa1 4 c3nsnA_ 5 d1ug3a2 6 d1qbaa3 7 c1qbaA_ 8 c2pnvA_ 9 c3rcnA_ 10 c2qzcB_ 11 c2rbfB_ 12 c1m04A_ 13 d2ahma1 14 d1ysya1 15 d1jaka1 16 c2o59B_ 17 d2p62a1 18 c2p58A_ 19 d1i7da_ 20 d1v4aa1 21 22 23 24 25 26 27 28 29 30 31

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 c3kk4B_ 87.0 29 PDB header:structural genomics, unknown function Chain: B: PDB Molecule:uncharacterized protein bp1543; PDBTitle: uncharacterized protein bp1543 from bordetella pertussis tohama i 2 d2ay0a1 32.6 24 Fold:Ribbon-helix-helix Superfamily:Ribbon-helix-helix Family:PutA pre-N-terminal region-like 3 d2auaa1 22.7 40 Fold:ADP-ribosylation Superfamily:ADP-ribosylation Family:BC2332-like 4 c3nsnA_ 21.0 44 PDB header:hydrolase Chain: A: PDB Molecule:n-acetylglucosaminidase; PDBTitle: crystal structure of insect beta-n-acetyl-d-hexosaminidase ofhex12 complexed with tmg-chitotriomycin 5 d1ug3a2 20.2 18 Fold:alpha-alpha superhelix Superfamily:ARM repeat Family:MIF4G domain-like 6 d1qbaa3 14.4 24 Fold:TIM beta/alpha-barrel Superfamily:(Trans)glycosidases Family:beta-N-acetylhexosaminidase catalytic domain 7 c1qbaA_ 13.8 25 PDB header:glycosyl hydrolase Chain: A: PDB Molecule:chitobiase; PDBTitle: bacterial chitobiase, glycosyl hydrolase family 20 8 c2pnvA_ 12.3 22 PDB header:membrane protein Chain: A: PDB Molecule:small conductance calcium-activated potassium PDBTitle: crystal structure of the leucine zipper domain of small-2 conductance ca2+-activated k+ (skca) channel from rattus3 norvegicus 9 c3rcnA_ 12.2 19 PDB header:hydrolase Chain: A: PDB Molecule:beta-n-acetylhexosaminidase; PDBTitle: crystal structure of beta-n-acetylhexosaminidase from arthrobacter2 aurescens 10 c2qzcB_ 11.5 16 PDB header:lyase Chain: B: PDB Molecule:transcriptional activator tena-1; PDBTitle: crystal structure of a putative tena-like thiaminase (tena-1, sso2206)2 from sulfolobus solfataricus p2 at 1.50 a resolution 11 c2rbfB_ 10.7 25 PDB header:oxidoreductase/dna Chain: B: PDB Molecule:bifunctional protein puta; PDBTitle: structure of the ribbon-helix-helix domain of escherichia coli puta2 (puta52) complexed with operator dna (o2) 12 c1m04A_ 10.6 29 PDB header:hydrolase Chain: A: PDB Molecule:beta-n-acetylhexosaminidase; PDBTitle: mutant streptomyces plicatus beta-hexosaminidase (d313n) in complex2 with product (glcnac) 13 d2ahma1 10.5 35 Fold:immunoglobulin/albumin-binding domain-like Superfamily:Coronavirus NSP7-like Family:Coronavirus NSP7-like 14 d1ysya1 10.2 35 Fold:immunoglobulin/albumin-binding domain-like Superfamily:Coronavirus NSP7-like Family:Coronavirus NSP7-like 15 d1jaka1 9.4 31 Fold:TIM beta/alpha-barrel Superfamily:(Trans)glycosidases Family:beta-N-acetylhexosaminidase catalytic domain 16 c2o59B_ 8.4 23 PDB header:isomerase/dna Chain: B: PDB Molecule:dna topoisomerase 3; PDBTitle: structure of e. coli topoisomerase iii in complex with an 8-2 base single stranded oligonucleotide. frozen in glycerol3 ph 8.0 17 d2p62a1 8.3 31 Fold:PH0156-like Superfamily:PH0156-like Family:PH0156-like 18 c2p58A_ 7.9 44 PDB header:transport protein/chaperone Chain: A: PDB Molecule:putative type iii secretion protein ysce; PDBTitle: structure of the yersinia pestis type iii secretion system2 needle protein yscf in complex with its chaperones3 ysce/yscg 19 d1i7da_ 7.9 23 Fold:Prokaryotic type I DNA topoisomerase Superfamily:Prokaryotic type I DNA topoisomerase Family:Prokaryotic type I DNA topoisomerase 20 d1v4aa1 7.5 21 Fold:Four-helical up-and-down bundle Superfamily:Nucleotidyltransferase substrate binding subunit/domain Family:Glutamine synthase adenylyltransferase GlnE, domain 2 21 d2cpga_ not modelled 7.2 28 Fold:Ribbon-helix-helix Superfamily:Ribbon-helix-helix Family:CopG-like 22 c3fshC_ not modelled 7.0 39 PDB header:ligase Chain: C: PDB Molecule:autocrine motility factor receptor, isoform 2; PDBTitle: crystal structure of the ubiquitin conjugating enzyme2 ube2g2 bound to the g2br domain of ubiquitin ligase gp78 23 c3lmyA_ not modelled 6.5 25 PDB header:hydrolase Chain: A: PDB Molecule:beta-hexosaminidase subunit beta; PDBTitle: the crystal structure of beta-hexosaminidase b in complex with2 pyrimethamine 24 c3h8kB_ not modelled 6.4 39 PDB header:ligase Chain: B: PDB Molecule:autocrine motility factor receptor, isoform 2; PDBTitle: crystal structure of ube2g2 complxed with the g2br domain of2 gp78 at 1.8-a resolution 25 d1nowa1 not modelled 6.2 24 Fold:TIM beta/alpha-barrel Superfamily:(Trans)glycosidases Family:beta-N-acetylhexosaminidase catalytic domain 26 c1zw3B_ not modelled 5.7 58 PDB header:protein binding Chain: B: PDB Molecule:talin 1; PDBTitle: vinculin head (0-258) in complex with the talin rod2 residues 1630-1652 27 c1nouA_ not modelled 5.5 24 PDB header:hydrolase Chain: A: PDB Molecule:beta-hexosaminidase beta chain; PDBTitle: native human lysosomal beta-hexosaminidase isoform b 28 c2ykqC_ not modelled 5.4 21 PDB header:rna-binding protein Chain: C: PDB Molecule:line-1 orf1p; PDBTitle: structure of the human line-1 orf1p trimer 29 d1ukfa_ not modelled 5.4 11 Fold:Cysteine proteinases Superfamily:Cysteine proteinases Family:Avirulence protein Avrpph3 30 d1g73a_ not modelled 5.3 37 Fold:Spectrin repeat-like Superfamily:Smac/diablo Family:Smac/diablo 31 d1fewa_ not modelled 5.0 37 Fold:Spectrin repeat-like Superfamily:Smac/diablo Family:Smac/diablo

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0