Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	F	E	I	T	G	I	N	V	S	G	A	L	K	A	V	V	M	A	T	G	F	E	N	P	L	S	S	V	N	E	I	E	T	K	L	S	A	L	L	G	S	E	T	T	G	E	I	L	F	D	L	L	C	A	N	G	P	E	W
Secondary structure
SS confidence
Disorder		?	?	?	?	?				?																														?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		N	R	F	V	T	L	E	M	K	Y	G	R	I	M	L	D	T	A	K	I	I	D	E	Q	D	V	P	T	H	I	L	S	K	L	T	F	T	L	R	N	H	P	E	Y	L	E	A	S	V	L	S	P	D	D	V	R	Q	V	L	S
Secondary structure
SS confidence
Disorder											?					?	?						?	?			?	?																																?	?
Disorder confidence
		.	.	.
Sequence		M	D	F
Secondary structure
SS confidence
Disorder		?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 1v8h chain A domain 1 Region: 43 - 72 Aligned: 30 Modelled: 30 Confidence: 29.3% Identity: 13% Fold: Immunoglobulin-like beta-sandwich Superfamily: E set domains Family: SoxZ-like Phyre2 PDB 1mgt chain A Region: 1 - 119 Aligned: 113 Modelled: 119 Confidence: 16.1% Identity: 12% PDB header:transferase Chain: A: PDB Molecule:protein (o6-methylguanine-dna methyltransferase); PDBTitle: crystal structure of o6-methylguanine-dna methyltransferase from2 hyperthermophilic archaeon pyrococcus kodakaraensis strain kod1 Phyre2 PDB 3lf4 chain B Region: 54 - 80 Aligned: 25 Modelled: 27 Confidence: 9.9% Identity: 20% PDB header:fluorescent protein Chain: B: PDB Molecule:fluorescent timer precursor blue102; PDBTitle: crystal structure of fluorescent timer precursor blue102 Phyre2 PDB 2gw4 chain D Region: 54 - 80 Aligned: 25 Modelled: 27 Confidence: 9.6% Identity: 28% PDB header:luminescent protein Chain: D: PDB Molecule:kaede; PDBTitle: crystal structure of stony coral fluorescent protein kaede, red form Phyre2 PDB 2zmw chain C Region: 54 - 80 Aligned: 25 Modelled: 27 Confidence: 8.8% Identity: 32% PDB header:luminescent protein Chain: C: PDB Molecule:fluorescent protein; PDBTitle: crystal structure of monomeric kusabira-orange (mko),2 orange-emitting gfp-like protein, at ph 6.0 Phyre2 PDB 1mou chain A Region: 54 - 80 Aligned: 25 Modelled: 27 Confidence: 8.8% Identity: 16% Fold: GFP-like Superfamily: GFP-like Family: Fluorescent proteins Phyre2 PDB 1w2t chain E Region: 52 - 82 Aligned: 31 Modelled: 31 Confidence: 8.3% Identity: 16% PDB header:hydrolase Chain: E: PDB Molecule:beta fructosidase; PDBTitle: beta-fructosidase from thermotoga maritima in complex with2 raffinose Phyre2 PDB 3evp chain A Region: 54 - 80 Aligned: 25 Modelled: 27 Confidence: 8.0% Identity: 20% PDB header:signaling protein Chain: A: PDB Molecule:circular-permutated green fluorescent protein; PDBTitle: crystal structure of circular-permutated egfp Phyre2 PDB 3cfh chain B Region: 50 - 80 Aligned: 29 Modelled: 31 Confidence: 7.8% Identity: 21% PDB header:fluorescent protein Chain: B: PDB Molecule:gfp-like photoswitchable fluorescent protein; PDBTitle: photoswitchable red fluorescent protein psrfp, off-state Phyre2 PDB 2rh7 chain A domain 1 Region: 54 - 80 Aligned: 25 Modelled: 27 Confidence: 7.8% Identity: 16% Fold: GFP-like Superfamily: GFP-like Family: Fluorescent proteins Phyre2 PDB 1lc0 chain A domain 2 Region: 57 - 75 Aligned: 19 Modelled: 19 Confidence: 7.8% Identity: 26% Fold: FwdE/GAPDH domain-like Superfamily: Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain Family: Biliverdin reductase Phyre2 PDB 2b5e chain A domain 3 Region: 10 - 50 Aligned: 36 Modelled: 41 Confidence: 7.7% Identity: 19% Fold: Thioredoxin fold Superfamily: Thioredoxin-like Family: PDI-like Phyre2 PDB 2z6z chain A Region: 54 - 80 Aligned: 25 Modelled: 27 Confidence: 7.7% Identity: 28% PDB header:fluorescent protein Chain: A: PDB Molecule:fluorescent protein dronpa; PDBTitle: crystal structure of a photoswitchable gfp-like protein2 dronpa in the bright-state Phyre2 PDB 1xqm chain A Region: 54 - 80 Aligned: 25 Modelled: 27 Confidence: 7.7% Identity: 20% Fold: GFP-like Superfamily: GFP-like Family: Fluorescent proteins Phyre2 PDB 1ggx chain A Region: 54 - 80 Aligned: 25 Modelled: 27 Confidence: 7.5% Identity: 20% Fold: GFP-like Superfamily: GFP-like Family: Fluorescent proteins Phyre2 PDB 2c9j chain G Region: 54 - 80 Aligned: 25 Modelled: 27 Confidence: 7.5% Identity: 24% PDB header:luminescent protein Chain: G: PDB Molecule:green fluorescent protein fp512; PDBTitle: structure of the fluorescent protein cmfp512 at 1.35a from2 cerianthus membranaceus Phyre2 PDB 2otb chain B Region: 54 - 80 Aligned: 25 Modelled: 27 Confidence: 7.5% Identity: 24% PDB header:fluorescent protein Chain: B: PDB Molecule:gfp-like fluorescent chromoprotein cfp484; PDBTitle: crystal structure of a monomeric cyan fluorescent protein2 in the fluorescent state Phyre2 PDB 3ai5 chain A Region: 54 - 80 Aligned: 25 Modelled: 27 Confidence: 7.3% Identity: 20% PDB header:fluorescent protein, transcription Chain: A: PDB Molecule:yeast enhanced green fluorescent protein, ubiquitin; PDBTitle: crystal structure of yeast enhanced green fluorescent protein-2 ubiquitin fusion protein Phyre2 PDB 3gb3 chain B Region: 54 - 80 Aligned: 25 Modelled: 27 Confidence: 7.3% Identity: 16% PDB header:fluorescent protein Chain: B: PDB Molecule:killerred; PDBTitle: x-ray structure of genetically encoded photosensitizer2 killerred in native form Phyre2 PDB 1yzw chain B Region: 54 - 80 Aligned: 25 Modelled: 27 Confidence: 7.3% Identity: 24% PDB header:luminescent protein Chain: B: PDB Molecule:gfp-like non-fluorescent chromoprotein; PDBTitle: the 2.1a crystal structure of the far-red fluorescent2 protein hcred: inherent conformational flexibility of the3 chromophore Phyre2 1 d1v8ha1 2 c1mgtA_ 3 c3lf4B_ 4 c2gw4D_ 5 c2zmwC_ 6 d1moua_ 7 c1w2tE_ 8 c3evpA_ 9 c3cfhB_ 10 d2rh7a1 11 d1lc0a2 12 d2b5ea3 13 c2z6zA_ 14 d1xqma_ 15 d1ggxa_ 16 c2c9jG_ 17 c2otbB_ 18 c3ai5A_ 19 c3gb3B_ 20 c1yzwB_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 d1v8ha1 29.3 13 Fold:Immunoglobulin-like beta-sandwich Superfamily:E set domains Family:SoxZ-like 2 c1mgtA_ 16.1 12 PDB header:transferase Chain: A: PDB Molecule:protein (o6-methylguanine-dna methyltransferase); PDBTitle: crystal structure of o6-methylguanine-dna methyltransferase from2 hyperthermophilic archaeon pyrococcus kodakaraensis strain kod1 3 c3lf4B_ 9.9 20 PDB header:fluorescent protein Chain: B: PDB Molecule:fluorescent timer precursor blue102; PDBTitle: crystal structure of fluorescent timer precursor blue102 4 c2gw4D_ 9.6 28 PDB header:luminescent protein Chain: D: PDB Molecule:kaede; PDBTitle: crystal structure of stony coral fluorescent protein kaede, red form 5 c2zmwC_ 8.8 32 PDB header:luminescent protein Chain: C: PDB Molecule:fluorescent protein; PDBTitle: crystal structure of monomeric kusabira-orange (mko),2 orange-emitting gfp-like protein, at ph 6.0 6 d1moua_ 8.8 16 Fold:GFP-like Superfamily:GFP-like Family:Fluorescent proteins 7 c1w2tE_ 8.3 16 PDB header:hydrolase Chain: E: PDB Molecule:beta fructosidase; PDBTitle: beta-fructosidase from thermotoga maritima in complex with2 raffinose 8 c3evpA_ 8.0 20 PDB header:signaling protein Chain: A: PDB Molecule:circular-permutated green fluorescent protein; PDBTitle: crystal structure of circular-permutated egfp 9 c3cfhB_ 7.8 21 PDB header:fluorescent protein Chain: B: PDB Molecule:gfp-like photoswitchable fluorescent protein; PDBTitle: photoswitchable red fluorescent protein psrfp, off-state 10 d2rh7a1 7.8 16 Fold:GFP-like Superfamily:GFP-like Family:Fluorescent proteins 11 d1lc0a2 7.8 26 Fold:FwdE/GAPDH domain-like Superfamily:Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain Family:Biliverdin reductase 12 d2b5ea3 7.7 19 Fold:Thioredoxin fold Superfamily:Thioredoxin-like Family:PDI-like 13 c2z6zA_ 7.7 28 PDB header:fluorescent protein Chain: A: PDB Molecule:fluorescent protein dronpa; PDBTitle: crystal structure of a photoswitchable gfp-like protein2 dronpa in the bright-state 14 d1xqma_ 7.7 20 Fold:GFP-like Superfamily:GFP-like Family:Fluorescent proteins 15 d1ggxa_ 7.5 20 Fold:GFP-like Superfamily:GFP-like Family:Fluorescent proteins 16 c2c9jG_ 7.5 24 PDB header:luminescent protein Chain: G: PDB Molecule:green fluorescent protein fp512; PDBTitle: structure of the fluorescent protein cmfp512 at 1.35a from2 cerianthus membranaceus 17 c2otbB_ 7.5 24 PDB header:fluorescent protein Chain: B: PDB Molecule:gfp-like fluorescent chromoprotein cfp484; PDBTitle: crystal structure of a monomeric cyan fluorescent protein2 in the fluorescent state 18 c3ai5A_ 7.3 20 PDB header:fluorescent protein, transcription Chain: A: PDB Molecule:yeast enhanced green fluorescent protein, ubiquitin; PDBTitle: crystal structure of yeast enhanced green fluorescent protein-2 ubiquitin fusion protein 19 c3gb3B_ 7.3 16 PDB header:fluorescent protein Chain: B: PDB Molecule:killerred; PDBTitle: x-ray structure of genetically encoded photosensitizer2 killerred in native form 20 c1yzwB_ 7.3 24 PDB header:luminescent protein Chain: B: PDB Molecule:gfp-like non-fluorescent chromoprotein; PDBTitle: the 2.1a crystal structure of the far-red fluorescent2 protein hcred: inherent conformational flexibility of the3 chromophore 21 d1vkna2 not modelled 7.1 30 Fold:FwdE/GAPDH domain-like Superfamily:Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain Family:GAPDH-like 22 c3rwaE_ not modelled 7.1 20 PDB header:fluorescent protein Chain: E: PDB Molecule:fluorescent protein fp480; PDBTitle: crystal structure of circular-permutated mkate 23 d1ne2a_ not modelled 6.9 18 Fold:S-adenosyl-L-methionine-dependent methyltransferases Superfamily:S-adenosyl-L-methionine-dependent methyltransferases Family:Ta1320-like 24 c3akoG_ not modelled 6.9 20 PDB header:fluorescent protein Chain: G: PDB Molecule:venus; PDBTitle: crystal structure of the reassembled venus 25 d2q49a2 not modelled 6.9 40 Fold:FwdE/GAPDH domain-like Superfamily:Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain Family:GAPDH-like 26 c2oxgE_ not modelled 6.5 14 PDB header:transport protein Chain: E: PDB Molecule:soxz protein; PDBTitle: the soxyz complex of paracoccus pantotrophus 27 c2g3dB_ not modelled 6.4 20 PDB header:luminescent protein Chain: B: PDB Molecule:green fluorescent protein; PDBTitle: structure of s65g y66a gfp variant after spontaneous2 peptide hydrolysis 28 c3cglE_ not modelled 5.9 24 PDB header:fluorescent protein Chain: E: PDB Molecule:gfp-like fluorescent chromoprotein dsfp483; PDBTitle: crystal structure and raman studies of dsfp483, a cyan fluorescent2 protein from discosoma striata 29 c3t9aA_ not modelled 5.9 25 PDB header:transferase Chain: A: PDB Molecule:inositol pyrophosphate kinase; PDBTitle: crystal structure of the catalytic domain of human diphosphoinositol2 pentakisphosphate kinase 2 (ppip5k2) in complex with amppnp at ph 7.0 30 c2ib5H_ not modelled 5.8 20 PDB header:luminescent protein Chain: H: PDB Molecule:chromo protein; PDBTitle: structural characterization of a blue chromoprotein and its yellow2 mutant from the sea anemone cnidopus japonicus 31 d1nh8a2 not modelled 5.7 25 Fold:Ferredoxin-like Superfamily:GlnB-like Family:ATP phosphoribosyltransferase (ATP-PRTase, HisG), regulatory C-terminal domain 32 d1ckma2 not modelled 5.4 40 Fold:ATP-grasp Superfamily:DNA ligase/mRNA capping enzyme, catalytic domain Family:mRNA capping enzyme 33 d2v0ea1 not modelled 5.4 30 Fold:GYF/BRK domain-like Superfamily:BRK domain-like Family:BRK domain-like 34 d1mywa_ not modelled 5.3 20 Fold:GFP-like Superfamily:GFP-like Family:Fluorescent proteins 35 d1uisa_ not modelled 5.3 16 Fold:GFP-like Superfamily:GFP-like Family:Fluorescent proteins

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0