Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	S	K	H	D	T	D	T	S	D	Q	H	A	A	K	R	R	W	L	N	A	H	E	E	G	Y	H	K	A	M	G	N	R	Q	V	Q	M	I	A	I	G	G	A	I	G	T	G	L	F	L	G	A	G	A	R	L	Q	M	A	G
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		P	A	L	A	L	V	Y	L	I	C	G	L	F	S	F	F	I	L	R	A	L	G	E	L	V	L	H	R	P	S	S	G	S	F	V	S	Y	A	R	E	F	L	G	E	K	A	A	Y	V	A	G	W	M	Y	F	I	N	W	A	M
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		T	G	I	V	D	I	T	A	V	A	L	Y	M	H	Y	W	G	A	F	G	G	V	P	Q	W	V	F	A	L	A	A	L	T	I	V	G	T	M	N	M	I	G	V	K	W	F	A	E	M	E	F	W	F	A	L	I	K	V	L	A
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		I	V	T	F	L	V	V	G	T	V	F	L	G	S	G	Q	P	L	D	G	N	T	T	G	F	H	L	I	T	D	N	G	G	F	F	P	H	G	L	L	P	A	L	V	L	I	Q	G	V	V	F	A	F	A	S	I	E	M	V	G
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		T	A	A	G	E	C	K	D	P	Q	T	M	V	P	K	A	I	N	S	V	I	W	R	I	G	L	F	Y	V	G	S	V	V	L	L	V	M	L	L	P	W	S	A	Y	Q	A	G	Q	S	P	F	V	T	F	F	S	K	L	G	V
Secondary structure
SS confidence
Disorder										?																																	?	?		?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		P	Y	I	G	S	I	M	N	I	V	V	L	T	A	A	L	S	S	L	N	S	G	L	Y	C	T	G	R	I	L	R	S	M	A	M	G	G	S	A	P	S	F	M	A	K	M	S	R	Q	H	V	P	Y	A	G	I	L	A	T	L
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420
Sequence		V	V	Y	V	V	G	V	F	L	N	Y	L	V	P	S	R	V	F	E	I	V	L	N	F	A	S	L	G	I	I	A	S	W	A	F	I	I	V	C	Q	M	R	L	R	K	A	I	K	E	G	K	A	A	D	V	S	F	K	L	P
Secondary structure
SS confidence
Disorder													?																																				?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.	.	.	.	.	.	.	460	.	.	.	.	.	.	.	.	.	470	.	.	.	.	.	.	.	.	.	480
Sequence		G	A	P	F	T	S	W	L	T	L	L	F	L	L	S	V	L	V	L	M	A	F	D	Y	P	N	G	T	Y	T	I	A	A	L	P	I	I	G	I	L	L	V	I	G	W	F	G	V	R	K	R	V	A	E	I	H	S	T	A	P
Secondary structure
SS confidence
Disorder																																																					?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	490	.	.	.	.	.	.	.	.	.
Sequence		V	V	E	E	D	E	E	K	Q	E	I	V	F	K	P	E	T	A	S
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 3gia chain A Region: 27 - 478 Aligned: 424 Modelled: 424 Confidence: 100.0% Identity: 17% PDB header:transport protein Chain: A: PDB Molecule:uncharacterized protein mj0609; PDBTitle: crystal structure of apct transporter Phyre2 PDB 3lrc chain C Region: 27 - 475 Aligned: 406 Modelled: 406 Confidence: 100.0% Identity: 17% PDB header:transport protein Chain: C: PDB Molecule:arginine/agmatine antiporter; PDBTitle: structure of e. coli adic (p1) Phyre2 PDB 2jln chain A Region: 11 - 488 Aligned: 457 Modelled: 476 Confidence: 100.0% Identity: 11% PDB header:membrane protein Chain: A: PDB Molecule:mhp1; PDBTitle: structure of mhp1, a nucleobase-cation-symport-1 family2 transporter Phyre2 PDB 2xq2 chain A Region: 25 - 483 Aligned: 440 Modelled: 440 Confidence: 99.4% Identity: 11% PDB header:transport protein Chain: A: PDB Molecule:sodium/glucose cotransporter; PDBTitle: structure of the k294a mutant of vsglt Phyre2 PDB 3dh4 chain A Region: 9 - 486 Aligned: 460 Modelled: 443 Confidence: 99.4% Identity: 9% PDB header:transport protein Chain: A: PDB Molecule:sodium/glucose cotransporter; PDBTitle: crystal structure of sodium/sugar symporter with bound galactose from2 vibrio parahaemolyticus Phyre2 PDB 2a65 chain A domain 1 Region: 31 - 474 Aligned: 434 Modelled: 434 Confidence: 98.3% Identity: 9% Fold: SNF-like Superfamily: SNF-like Family: SNF-like Phyre2 PDB 2w8a chain C Region: 23 - 410 Aligned: 377 Modelled: 379 Confidence: 97.9% Identity: 12% PDB header:membrane protein Chain: C: PDB Molecule:glycine betaine transporter betp; PDBTitle: crystal structure of the sodium-coupled glycine betaine2 symporter betp from corynebacterium glutamicum with bound3 substrate Phyre2 PDB 3hfx chain A Region: 102 - 409 Aligned: 300 Modelled: 308 Confidence: 96.2% Identity: 8% PDB header:transport protein Chain: A: PDB Molecule:l-carnitine/gamma-butyrobetaine antiporter; PDBTitle: crystal structure of carnitine transporter Phyre2 PDB 2w2e chain A Region: 2 - 249 Aligned: 248 Modelled: 248 Confidence: 49.6% Identity: 11% PDB header:membrane protein Chain: A: PDB Molecule:aquaporin; PDBTitle: 1.15 angstrom crystal structure of p.pastoris aquaporin,2 aqy1, in a closed conformation at ph 3.5 Phyre2 PDB 1fft chain B domain 2 Region: 375 - 441 Aligned: 67 Modelled: 67 Confidence: 35.6% Identity: 12% Fold: Transmembrane helix hairpin Superfamily: Cytochrome c oxidase subunit II-like, transmembrane region Family: Cytochrome c oxidase subunit II-like, transmembrane region Phyre2 PDB 2knc chain A Region: 443 - 488 Aligned: 46 Modelled: 46 Confidence: 30.3% Identity: 9% PDB header:cell adhesion Chain: A: PDB Molecule:integrin alpha-iib; PDBTitle: platelet integrin alfaiib-beta3 transmembrane-cytoplasmic2 heterocomplex Phyre2 PDB 2fb5 chain A domain 1 Region: 7 - 41 Aligned: 35 Modelled: 35 Confidence: 15.0% Identity: 11% Fold: YojJ-like Superfamily: YojJ-like Family: YojJ-like Phyre2 PDB 3m7b chain A Region: 352 - 480 Aligned: 129 Modelled: 129 Confidence: 13.9% Identity: 13% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:tellurite resistance protein teha homolog; PDBTitle: crystal structure of plant slac1 homolog teha Phyre2 PDB 2bbj chain B Region: 424 - 472 Aligned: 49 Modelled: 49 Confidence: 12.3% Identity: 12% PDB header:metal transport/membrane protein Chain: B: PDB Molecule:divalent cation transport-related protein; PDBTitle: crystal structure of the cora mg2+ transporter Phyre2 PDB 3o7p chain A Region: 347 - 499 Aligned: 153 Modelled: 153 Confidence: 9.7% Identity: 5% PDB header:transport protein Chain: A: PDB Molecule:l-fucose-proton symporter; PDBTitle: crystal structure of the e.coli fucose:proton symporter, fucp (n162a) Phyre2 PDB 3ent chain B Region: 11 - 31 Aligned: 21 Modelled: 21 Confidence: 8.3% Identity: 24% PDB header:structural protein Chain: B: PDB Molecule:putative uncharacterized protein; PDBTitle: crystal structure of nitrollin, a betagamma-crystallin from2 nitrosospira multiformis-in alternate space group (p65) Phyre2 PDB 3hd6 chain A Region: 317 - 499 Aligned: 172 Modelled: 183 Confidence: 8.2% Identity: 12% PDB header:membrane protein, transport protein Chain: A: PDB Molecule:ammonium transporter rh type c; PDBTitle: crystal structure of the human rhesus glycoprotein rhcg Phyre2 PDB 3p5t chain E Region: 2 - 29 Aligned: 28 Modelled: 28 Confidence: 7.1% Identity: 14% PDB header:rna binding protein Chain: E: PDB Molecule:cleavage and polyadenylation specificity factor subunit 5; PDBTitle: cfim25-cfim68 complex Phyre2 PDB 3qnq chain D Region: 435 - 497 Aligned: 53 Modelled: 63 Confidence: 6.9% Identity: 11% PDB header:membrane protein, transport protein Chain: D: PDB Molecule:pts system, cellobiose-specific iic component; PDBTitle: crystal structure of the transporter chbc, the iic component from the2 n,n'-diacetylchitobiose-specific phosphotransferase system Phyre2 PDB 3ixz chain A Region: 386 - 474 Aligned: 89 Modelled: 89 Confidence: 5.9% Identity: 4% PDB header:hydrolase Chain: A: PDB Molecule:potassium-transporting atpase alpha; PDBTitle: pig gastric h+/k+-atpase complexed with aluminium fluoride Phyre2 1 c3giaA_ 2 c3lrcC_ 3 c2jlnA_ 4 c2xq2A_ 5 c3dh4A_ 6 d2a65a1 7 c2w8aC_ 8 c3hfxA_ 9 c2w2eA_ 10 d1fftb2 11 c2kncA_ 12 d2fb5a1 13 c3m7bA_ 14 c2bbjB_ 15 c3o7pA_ 16 c3entB_ 17 c3hd6A_ 18 c3p5tE_ 19 c3qnqD_ 20 c3ixzA_ 21 22 23 24 25 26 27 28 29

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 c3giaA_ 100.0 17 PDB header:transport protein Chain: A: PDB Molecule:uncharacterized protein mj0609; PDBTitle: crystal structure of apct transporter 2 c3lrcC_ 100.0 17 PDB header:transport protein Chain: C: PDB Molecule:arginine/agmatine antiporter; PDBTitle: structure of e. coli adic (p1) 3 c2jlnA_ 100.0 11 PDB header:membrane protein Chain: A: PDB Molecule:mhp1; PDBTitle: structure of mhp1, a nucleobase-cation-symport-1 family2 transporter 4 c2xq2A_ 99.4 11 PDB header:transport protein Chain: A: PDB Molecule:sodium/glucose cotransporter; PDBTitle: structure of the k294a mutant of vsglt 5 c3dh4A_ 99.4 9 PDB header:transport protein Chain: A: PDB Molecule:sodium/glucose cotransporter; PDBTitle: crystal structure of sodium/sugar symporter with bound galactose from2 vibrio parahaemolyticus 6 d2a65a1 98.3 9 Fold:SNF-like Superfamily:SNF-like Family:SNF-like 7 c2w8aC_ 97.9 12 PDB header:membrane protein Chain: C: PDB Molecule:glycine betaine transporter betp; PDBTitle: crystal structure of the sodium-coupled glycine betaine2 symporter betp from corynebacterium glutamicum with bound3 substrate 8 c3hfxA_ 96.2 8 PDB header:transport protein Chain: A: PDB Molecule:l-carnitine/gamma-butyrobetaine antiporter; PDBTitle: crystal structure of carnitine transporter 9 c2w2eA_ 49.6 11 PDB header:membrane protein Chain: A: PDB Molecule:aquaporin; PDBTitle: 1.15 angstrom crystal structure of p.pastoris aquaporin,2 aqy1, in a closed conformation at ph 3.5 10 d1fftb2 35.6 12 Fold:Transmembrane helix hairpin Superfamily:Cytochrome c oxidase subunit II-like, transmembrane region Family:Cytochrome c oxidase subunit II-like, transmembrane region 11 c2kncA_ 30.3 9 PDB header:cell adhesion Chain: A: PDB Molecule:integrin alpha-iib; PDBTitle: platelet integrin alfaiib-beta3 transmembrane-cytoplasmic2 heterocomplex 12 d2fb5a1 15.0 11 Fold:YojJ-like Superfamily:YojJ-like Family:YojJ-like 13 c3m7bA_ 13.9 13 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:tellurite resistance protein teha homolog; PDBTitle: crystal structure of plant slac1 homolog teha 14 c2bbjB_ 12.3 12 PDB header:metal transport/membrane protein Chain: B: PDB Molecule:divalent cation transport-related protein; PDBTitle: crystal structure of the cora mg2+ transporter 15 c3o7pA_ 9.7 5 PDB header:transport protein Chain: A: PDB Molecule:l-fucose-proton symporter; PDBTitle: crystal structure of the e.coli fucose:proton symporter, fucp (n162a) 16 c3entB_ 8.3 24 PDB header:structural protein Chain: B: PDB Molecule:putative uncharacterized protein; PDBTitle: crystal structure of nitrollin, a betagamma-crystallin from2 nitrosospira multiformis-in alternate space group (p65) 17 c3hd6A_ 8.2 12 PDB header:membrane protein, transport protein Chain: A: PDB Molecule:ammonium transporter rh type c; PDBTitle: crystal structure of the human rhesus glycoprotein rhcg 18 c3p5tE_ 7.1 14 PDB header:rna binding protein Chain: E: PDB Molecule:cleavage and polyadenylation specificity factor subunit 5; PDBTitle: cfim25-cfim68 complex 19 c3qnqD_ 6.9 11 PDB header:membrane protein, transport protein Chain: D: PDB Molecule:pts system, cellobiose-specific iic component; PDBTitle: crystal structure of the transporter chbc, the iic component from the2 n,n'-diacetylchitobiose-specific phosphotransferase system 20 c3ixzA_ 5.9 4 PDB header:hydrolase Chain: A: PDB Molecule:potassium-transporting atpase alpha; PDBTitle: pig gastric h+/k+-atpase complexed with aluminium fluoride 21 c2jwaA_ not modelled 5.8 11 PDB header:transferase Chain: A: PDB Molecule:receptor tyrosine-protein kinase erbb-2; PDBTitle: erbb2 transmembrane segment dimer spatial structure 22 c2jo1A_ not modelled 5.8 24 PDB header:hydrolase regulator Chain: A: PDB Molecule:phospholemman; PDBTitle: structure of the na,k-atpase regulatory protein fxyd1 in2 micelles 23 c2xutC_ not modelled 5.8 9 PDB header:transport protein Chain: C: PDB Molecule:proton/peptide symporter family protein; PDBTitle: crystal structure of a proton dependent oligopeptide (pot)2 family transporter. 24 d1iyjb2 not modelled 5.7 26 Fold:BRCA2 tower domain Superfamily:BRCA2 tower domain Family:BRCA2 tower domain 25 d2oara1 not modelled 5.6 9 Fold:Gated mechanosensitive channel Superfamily:Gated mechanosensitive channel Family:Gated mechanosensitive channel 26 c2jp3A_ not modelled 5.5 19 PDB header:transcription Chain: A: PDB Molecule:fxyd domain-containing ion transport regulator 4; PDBTitle: solution structure of the human fxyd4 (chif) protein in sds2 micelles 27 c1m57H_ not modelled 5.4 11 PDB header:oxidoreductase Chain: H: PDB Molecule:cytochrome c oxidase; PDBTitle: structure of cytochrome c oxidase from rhodobacter2 sphaeroides (eq(i-286) mutant)) 28 d1mjta_ not modelled 5.3 11 Fold:Nitric oxide (NO) synthase oxygenase domain Superfamily:Nitric oxide (NO) synthase oxygenase domain Family:Nitric oxide (NO) synthase oxygenase domain 29 d2nr9a1 not modelled 5.2 16 Fold:Rhomboid-like Superfamily:Rhomboid-like Family:Rhomboid-like

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0