Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	S	M	P	L	S	N	A	L	Q	S	Q	I	I	T	D	A	H	F	L	H	H	P	I	V	D	S	E	F	T	R	K	L	K	Y	A	R	M	D	S	E	N	I	Y	L	P	P	L	T	R	G	N	N	H	N	Y	D	G	K	S
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?																																											?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		V	V	E	I	R	K	L	D	I	S	K	E	P	W	P	F	N	Y	V	T	G	A	C	R	E	S	D	G	I	T	T	T	G	R	M	L	Y	R	N	L	K	I	T	S	A	L	D	E	I	Y	G	G	I	C	K	K	A	H	A	T
Secondary structure
SS confidence
Disorder																														?								?																						?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		T	E	L	A	E	G	L	R	L	N	L	F	M	K	S	P	F	D	P	V	E	D	Y	T	V	H	E	I	T	L	G	P	G	C	N	V	P	G	Y	A	G	T	T	I	G	Y	I	S	T	L	P	A	S	Q	A	K	R	W	T	N
Secondary structure
SS confidence
Disorder		?	?												?	?																																		?	?			?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		E	Q	P	R	I	D	I	Y	I	D	Q	I	M	T	V	T	G	V	A	N	S	S	G	F	A	L	A	A	L	L	N	A	N	I	E	L	G	N	D	P	I	I	G	I	E	A	Y	P	G	T	A	E	I	H	A	K	M	G	Y	K
Secondary structure
SS confidence
Disorder															?	?	?															?																		?		?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.
Sequence		V	I	P	G	D	E	N	A	P	L	K	R	M	T	L	Q	P	S	S	L	P	E	L	F	E	L	K	N	G	E	W	N	Y	I	G	K
Secondary structure
SS confidence
Disorder																																	?	?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 2atr chain A domain 1 Region: 161 - 244 Aligned: 75 Modelled: 84 Confidence: 38.9% Identity: 13% Fold: Acyl-CoA N-acyltransferases (Nat) Superfamily: Acyl-CoA N-acyltransferases (Nat) Family: N-acetyl transferase, NAT Phyre2 PDB 3s6f chain A Region: 161 - 261 Aligned: 86 Modelled: 96 Confidence: 28.5% Identity: 15% PDB header:transferase Chain: A: PDB Molecule:hypothetical acetyltransferase; PDBTitle: crystal structure of a hypotetical acetyltransferase (dr_1678) from2 deinococcus radiodurans at 1.19 a resolution Phyre2 PDB 2odg chain C domain 1 Region: 5 - 33 Aligned: 27 Modelled: 29 Confidence: 24.2% Identity: 33% Fold: LEM/SAP HeH motif Superfamily: LEM domain Family: LEM domain Phyre2 PDB 1yf9 chain A domain 1 Region: 119 - 175 Aligned: 56 Modelled: 57 Confidence: 22.1% Identity: 11% Fold: UBC-like Superfamily: UBC-like Family: UBC-related Phyre2 PDB 1yx0 chain A domain 1 Region: 161 - 242 Aligned: 73 Modelled: 82 Confidence: 19.5% Identity: 14% Fold: Acyl-CoA N-acyltransferases (Nat) Superfamily: Acyl-CoA N-acyltransferases (Nat) Family: N-acetyl transferase, NAT Phyre2 PDB 2xr4 chain A Region: 179 - 191 Aligned: 13 Modelled: 13 Confidence: 18.2% Identity: 46% PDB header:sugar binding protein Chain: A: PDB Molecule:lectin; PDBTitle: c-terminal domain of bc2l-c lectin from burkholderia cenocepacia Phyre2 PDB 2cho chain A domain 2 Region: 139 - 175 Aligned: 37 Modelled: 37 Confidence: 17.7% Identity: 27% Fold: TIM beta/alpha-barrel Superfamily: (Trans)glycosidases Family: alpha-D-glucuronidase/Hyaluronidase catalytic domain Phyre2 PDB 1m2o chain A Region: 157 - 234 Aligned: 67 Modelled: 71 Confidence: 16.7% Identity: 21% PDB header:protein transport/signaling protein Chain: A: PDB Molecule:protein transport protein sec23; PDBTitle: crystal structure of the sec23-sar1 complex Phyre2 PDB 1j58 chain A Region: 243 - 274 Aligned: 32 Modelled: 31 Confidence: 15.5% Identity: 34% Fold: Double-stranded beta-helix Superfamily: RmlC-like cupins Family: Germin/Seed storage 7S protein Phyre2 PDB 3ijr chain F Region: 168 - 210 Aligned: 43 Modelled: 40 Confidence: 14.6% Identity: 16% PDB header:oxidoreductase Chain: F: PDB Molecule:oxidoreductase, short chain dehydrogenase/reductase family; PDBTitle: 2.05 angstrom resolution crystal structure of a short chain2 dehydrogenase from bacillus anthracis str. 'ames ancestor' in complex3 with nad+ Phyre2 PDB 1fjr chain A Region: 217 - 269 Aligned: 53 Modelled: 53 Confidence: 14.6% Identity: 19% Fold: Methuselah ectodomain Superfamily: Methuselah ectodomain Family: Methuselah ectodomain Phyre2 PDB 2vnv chain C Region: 179 - 191 Aligned: 13 Modelled: 13 Confidence: 14.3% Identity: 31% PDB header:sugar-binding protein Chain: C: PDB Molecule:bcla; PDBTitle: crystal structure of bcla lectin from burkholderia2 cenocepacia in complex with alpha-methyl-mannoside at 1.73 angstrom resolution Phyre2 PDB 2cbi chain A domain 2 Region: 139 - 175 Aligned: 37 Modelled: 37 Confidence: 14.2% Identity: 24% Fold: TIM beta/alpha-barrel Superfamily: (Trans)glycosidases Family: alpha-D-glucuronidase/Hyaluronidase catalytic domain Phyre2 PDB 2jvw chain A Region: 19 - 45 Aligned: 27 Modelled: 27 Confidence: 13.6% Identity: 52% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:uncharacterized protein; PDBTitle: solution nmr structure of uncharacterized protein q5e7h1 from vibrio2 fischeri. northeast structural genomics target vfr117 Phyre2 PDB 1y9w chain A domain 1 Region: 161 - 249 Aligned: 80 Modelled: 86 Confidence: 13.1% Identity: 18% Fold: Acyl-CoA N-acyltransferases (Nat) Superfamily: Acyl-CoA N-acyltransferases (Nat) Family: N-acetyl transferase, NAT Phyre2 PDB 1x87 chain A Region: 233 - 275 Aligned: 41 Modelled: 43 Confidence: 13.0% Identity: 29% Fold: Urocanase Superfamily: Urocanase Family: Urocanase Phyre2 PDB 2cs3 chain A domain 1 Region: 1 - 76 Aligned: 62 Modelled: 62 Confidence: 12.4% Identity: 29% Fold: RING/U-box Superfamily: RING/U-box Family: Variant RING domain Phyre2 PDB 1c4z chain D Region: 122 - 175 Aligned: 52 Modelled: 54 Confidence: 11.5% Identity: 15% Fold: UBC-like Superfamily: UBC-like Family: UBC-related Phyre2 PDB 2boi chain A Region: 179 - 191 Aligned: 13 Modelled: 13 Confidence: 11.3% Identity: 15% PDB header:lectin Chain: A: PDB Molecule:cv-iil lectin; PDBTitle: 1.1a structure of chromobacterium violaceum lectin cv2l in2 complex with alpha-methyl-fucoside Phyre2 PDB 2chh chain A domain 1 Region: 179 - 191 Aligned: 13 Modelled: 13 Confidence: 10.8% Identity: 15% Fold: Calcium-mediated lectin Superfamily: Calcium-mediated lectin Family: Calcium-mediated lectin Phyre2 1 d2atra1 2 c3s6fA_ 3 d2odgc1 4 d1yf9a1 5 d1yx0a1 6 c2xr4A_ 7 d2choa2 8 c1m2oA_ 9 d1j58a_ 10 c3ijrF_ 11 d1fjra_ 12 c2vnvC_ 13 d2cbia2 14 c2jvwA_ 15 d1y9wa1 16 d1x87a_ 17 d2cs3a1 18 d1c4zd_ 19 c2boiA_ 20 d2chha1 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 d2atra1 38.9 13 Fold:Acyl-CoA N-acyltransferases (Nat) Superfamily:Acyl-CoA N-acyltransferases (Nat) Family:N-acetyl transferase, NAT 2 c3s6fA_ 28.5 15 PDB header:transferase Chain: A: PDB Molecule:hypothetical acetyltransferase; PDBTitle: crystal structure of a hypotetical acetyltransferase (dr_1678) from2 deinococcus radiodurans at 1.19 a resolution 3 d2odgc1 24.2 33 Fold:LEM/SAP HeH motif Superfamily:LEM domain Family:LEM domain 4 d1yf9a1 22.1 11 Fold:UBC-like Superfamily:UBC-like Family:UBC-related 5 d1yx0a1 19.5 14 Fold:Acyl-CoA N-acyltransferases (Nat) Superfamily:Acyl-CoA N-acyltransferases (Nat) Family:N-acetyl transferase, NAT 6 c2xr4A_ 18.2 46 PDB header:sugar binding protein Chain: A: PDB Molecule:lectin; PDBTitle: c-terminal domain of bc2l-c lectin from burkholderia cenocepacia 7 d2choa2 17.7 27 Fold:TIM beta/alpha-barrel Superfamily:(Trans)glycosidases Family:alpha-D-glucuronidase/Hyaluronidase catalytic domain 8 c1m2oA_ 16.7 21 PDB header:protein transport/signaling protein Chain: A: PDB Molecule:protein transport protein sec23; PDBTitle: crystal structure of the sec23-sar1 complex 9 d1j58a_ 15.5 34 Fold:Double-stranded beta-helix Superfamily:RmlC-like cupins Family:Germin/Seed storage 7S protein 10 c3ijrF_ 14.6 16 PDB header:oxidoreductase Chain: F: PDB Molecule:oxidoreductase, short chain dehydrogenase/reductase family; PDBTitle: 2.05 angstrom resolution crystal structure of a short chain2 dehydrogenase from bacillus anthracis str. 'ames ancestor' in complex3 with nad+ 11 d1fjra_ 14.6 19 Fold:Methuselah ectodomain Superfamily:Methuselah ectodomain Family:Methuselah ectodomain 12 c2vnvC_ 14.3 31 PDB header:sugar-binding protein Chain: C: PDB Molecule:bcla; PDBTitle: crystal structure of bcla lectin from burkholderia2 cenocepacia in complex with alpha-methyl-mannoside at 1.73 angstrom resolution 13 d2cbia2 14.2 24 Fold:TIM beta/alpha-barrel Superfamily:(Trans)glycosidases Family:alpha-D-glucuronidase/Hyaluronidase catalytic domain 14 c2jvwA_ 13.6 52 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:uncharacterized protein; PDBTitle: solution nmr structure of uncharacterized protein q5e7h1 from vibrio2 fischeri. northeast structural genomics target vfr117 15 d1y9wa1 13.1 18 Fold:Acyl-CoA N-acyltransferases (Nat) Superfamily:Acyl-CoA N-acyltransferases (Nat) Family:N-acetyl transferase, NAT 16 d1x87a_ 13.0 29 Fold:Urocanase Superfamily:Urocanase Family:Urocanase 17 d2cs3a1 12.4 29 Fold:RING/U-box Superfamily:RING/U-box Family:Variant RING domain 18 d1c4zd_ 11.5 15 Fold:UBC-like Superfamily:UBC-like Family:UBC-related 19 c2boiA_ 11.3 15 PDB header:lectin Chain: A: PDB Molecule:cv-iil lectin; PDBTitle: 1.1a structure of chromobacterium violaceum lectin cv2l in2 complex with alpha-methyl-fucoside 20 d2chha1 10.8 15 Fold:Calcium-mediated lectin Superfamily:Calcium-mediated lectin Family:Calcium-mediated lectin 21 c3gemC_ not modelled 10.6 23 PDB header:oxidoreductase Chain: C: PDB Molecule:short chain dehydrogenase; PDBTitle: crystal structure of short-chain dehydrogenase from pseudomonas2 syringae 22 d2nvuc1 not modelled 10.4 11 Fold:UBC-like Superfamily:UBC-like Family:UBC-related 23 d1uzva_ not modelled 10.4 0 Fold:Calcium-mediated lectin Superfamily:Calcium-mediated lectin Family:Calcium-mediated lectin 24 c2xlfA_ not modelled 10.2 8 PDB header:metal binding protein Chain: A: PDB Molecule:sll1785 protein; PDBTitle: structure and metal-loading of a soluble periplasm cupro-protein:2 apo-cuca-closed (semet) 25 c3rl0j_ not modelled 9.6 50 PDB header:membrane protein/exocytosis Chain: J: PDB Molecule:syntaxin-1a; PDBTitle: truncated snare complex with complexin (p1) 26 d1k2wa_ not modelled 9.3 21 Fold:NAD(P)-binding Rossmann-fold domains Superfamily:NAD(P)-binding Rossmann-fold domains Family:Tyrosine-dependent oxidoreductases 27 c2xsbA_ not modelled 9.2 19 PDB header:hydrolase Chain: A: PDB Molecule:hyaluronoglucosaminidase; PDBTitle: ogoga pugnac complex 28 c3gzaB_ not modelled 9.0 13 PDB header:hydrolase Chain: B: PDB Molecule:putative alpha-l-fucosidase; PDBTitle: crystal structure of putative alpha-l-fucosidase (np_812709.1) from2 bacteroides thetaiotaomicron vpi-5482 at 1.60 a resolution 29 d1xeba_ not modelled 8.9 16 Fold:Acyl-CoA N-acyltransferases (Nat) Superfamily:Acyl-CoA N-acyltransferases (Nat) Family:N-acetyl transferase, NAT 30 c2vxkA_ not modelled 8.0 16 PDB header:transferase Chain: A: PDB Molecule:glucosamine 6-phosphate acetyltransferase; PDBTitle: structural comparison between aspergillus fumigatus and2 human gna1 31 d1hywa_ not modelled 7.8 33 Fold:gpW/XkdW-like Superfamily:Head-to-tail joining protein W, gpW Family:Head-to-tail joining protein W, gpW 32 c2du7C_ not modelled 7.6 17 PDB header:ligase Chain: C: PDB Molecule:o-phosphoseryl-trna synthetase; PDBTitle: crystal structure of methanococcus jannacshii o-phosphoseryl-trna2 synthetase 33 d1su3a1 not modelled 7.3 56 Fold:PGBD-like Superfamily:PGBD-like Family:MMP N-terminal domain 34 c2cbjA_ not modelled 7.2 24 PDB header:hydrolase Chain: A: PDB Molecule:hyaluronidase; PDBTitle: structure of the clostridium perfringens nagj family 842 glycoside hydrolase, a homologue of human o-glcnacase in3 complex with pugnac 35 c3o2uB_ not modelled 6.9 12 PDB header:ligase Chain: B: PDB Molecule:nedd8-conjugating enzyme ubc12; PDBTitle: s. cerevisiae ubc12 36 c3topA_ not modelled 6.6 23 PDB header:hydrolase/hydrolase inhibitor Chain: A: PDB Molecule:maltase-glucoamylase, intestinal; PDBTitle: crystral structure of the c-terminal subunit of human maltase-2 glucoamylase in complex with acarbose 37 c2qj8B_ not modelled 6.5 18 PDB header:hydrolase Chain: B: PDB Molecule:mlr6093 protein; PDBTitle: crystal structure of an aspartoacylase family protein (mlr6093) from2 mesorhizobium loti maff303099 at 2.00 a resolution 38 d1ed7a_ not modelled 6.5 42 Fold:WW domain-like Superfamily:Carbohydrate binding domain Family:Carbohydrate binding domain 39 c3pvlA_ not modelled 6.4 28 PDB header:motor protein/protein transport Chain: A: PDB Molecule:myosin viia isoform 1; PDBTitle: structure of myosin viia myth4-ferm-sh3 in complex with the cen1 of2 sans 40 d2ae6a1 not modelled 6.4 13 Fold:Acyl-CoA N-acyltransferases (Nat) Superfamily:Acyl-CoA N-acyltransferases (Nat) Family:N-acetyl transferase, NAT 41 d1fasa_ not modelled 6.4 50 Fold:Snake toxin-like Superfamily:Snake toxin-like Family:Snake venom toxins 42 d1o8ba1 not modelled 6.2 45 Fold:NagB/RpiA/CoA transferase-like Superfamily:NagB/RpiA/CoA transferase-like Family:D-ribose-5-phosphate isomerase (RpiA), catalytic domain 43 c1kilE_ not modelled 6.2 57 PDB header:membrane protein Chain: E: PDB Molecule:complexin i snare-complex binding region; PDBTitle: three-dimensional structure of the complexin/snare complex 44 d2jdca1 not modelled 6.1 15 Fold:Acyl-CoA N-acyltransferases (Nat) Superfamily:Acyl-CoA N-acyltransferases (Nat) Family:N-acetyl transferase, NAT 45 d2fiwa1 not modelled 5.9 25 Fold:Acyl-CoA N-acyltransferases (Nat) Superfamily:Acyl-CoA N-acyltransferases (Nat) Family:N-acetyl transferase, NAT 46 d1xs8a_ not modelled 5.9 38 Fold:YggX-like Superfamily:YggX-like Family:YggX-like 47 d1d6za3 not modelled 5.8 26 Fold:Cystatin-like Superfamily:Amine oxidase N-terminal region Family:Amine oxidase N-terminal region 48 d2gx8a1 not modelled 5.8 22 Fold:NIF3 (NGG1p interacting factor 3)-like Superfamily:NIF3 (NGG1p interacting factor 3)-like Family:NIF3 (NGG1p interacting factor 3)-like 49 d1slma1 not modelled 5.8 57 Fold:PGBD-like Superfamily:PGBD-like Family:MMP N-terminal domain 50 d1ejda_ not modelled 5.7 16 Fold:IF3-like Superfamily:EPT/RTPC-like Family:Enolpyruvate transferase, EPT 51 d1yvoa1 not modelled 5.6 16 Fold:Acyl-CoA N-acyltransferases (Nat) Superfamily:Acyl-CoA N-acyltransferases (Nat) Family:N-acetyl transferase, NAT 52 c3nvaB_ not modelled 5.5 20 PDB header:ligase Chain: B: PDB Molecule:ctp synthase; PDBTitle: dimeric form of ctp synthase from sulfolobus solfataricus 53 d1wzva1 not modelled 5.4 16 Fold:UBC-like Superfamily:UBC-like Family:UBC-related 54 d2qtva3 not modelled 5.2 17 Fold:vWA-like Superfamily:vWA-like Family:Trunk domain of Sec23/24 55 c2pdoG_ not modelled 5.1 18 PDB header:transferase Chain: G: PDB Molecule:acetyltransferase ypea; PDBTitle: crystal structure of the putative acetyltransferase of gnat family2 from shigella flexneri

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0