Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	N	R	D	S	F	Y	P	A	I	A	C	F	P	L	L	L	M	L	A	G	C	A	P	M	H	E	T	R	Q	A	L	S	Q	Q	T	P	A	A	Q	V	D	T	A	L	P	T	A	L	K	M	V	G	Q	T	X	Q	W	W	L
Secondary structure
SS confidence
Disorder		?	?	?	?	?															?		?	?	?		?	?		?	?	?			?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		E	Y	H	D	N	Q	L	T	S	L	I	N	N	A	L	Q	N	A	P	D	M	Q	V	A	E	Q	R	I	Q	L	A	E	A	Q	A	K	A	V	A	T	Q	D	G	P	Q	I	D	F	S	A	D	M	E	R	Q	K	M	S	A	E
Secondary structure
SS confidence
Disorder																																								?	?	?							?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		G	L	M	G	P	F	A	L	N	D	P	A	A	G	T	T	G	P	W	Y	T	N	G	T	F	G	L	T	A	G	W	H	L	D	I	W	G	K	N	R	A	E	V	T	A	R	L	G	T	V	K	A	R	A	A	E	R	E	Q	T
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		R	Q	L	L	A	G	S	V	A	R	L	Y	W	E	W	Q	T	Q	A	A	L	N	T	V	L	Q	Q	I	E	K	E	Q	N	T	I	I	A	T	D	R	Q	L	Y	Q	N	G	I	T	S	S	V	E	G	V	E	T	D	I	N	A
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		S	K	T	R	Q	Q	L	N	D	V	A	G	K	M	K	I	I	E	A	R	L	S	A	L	T	N	N	Q	T	K	S	L	K	L	K	P	V	A	L	P	K	V	A	S	Q	L	P	D	E	L	G	Y	S	L	L	A	R	R	A	D
Secondary structure
SS confidence
Disorder																															?		?	?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		L	Q	A	A	H	W	Y	V	E	S	S	L	S	T	I	D	A	A	K	A	A	F	Y	P	D	I	N	L	M	A	F	L	Q	Q	D	A	L	H	L	S	D	L	F	R	H	S	A	Q	Q	M	G	V	T	A	G	L	T	L	P	I
Secondary structure
SS confidence
Disorder																														?	?	?	?	?	?	?		?	?	?	?	?	?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420
Sequence		F	D	S	G	R	L	N	A	N	L	D	I	A	K	A	E	S	N	L	S	I	A	S	Y	N	K	A	V	V	E	A	V	N	D	V	A	R	A	A	S	Q	V	Q	T	L	A	E	K	N	Q	H	Q	A	Q	I	E	R	D	A	L
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.	.	.	.	.	.	.	460	.	.	.	.	.	.	.	.	.	470	.	.	.	.	.	.	.	.
Sequence		R	V	V	G	L	A	Q	A	R	F	N	A	G	I	I	A	G	S	R	V	S	E	A	R	I	P	A	L	R	E	R	A	N	G	L	L	L	Q	G	Q	W	L	D	A	S	I	Q	L	T	G	A	L	G	G	G	Y	K	R
Secondary structure
SS confidence
Disorder																																																					?	?	?	?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 1wp1 chain A Region: 22 - 478 Aligned: 448 Modelled: 448 Confidence: 100.0% Identity: 23% Fold: Outer membrane efflux proteins (OEP) Superfamily: Outer membrane efflux proteins (OEP) Family: Outer membrane efflux proteins (OEP) Phyre2 PDB 3pik chain A Region: 22 - 475 Aligned: 428 Modelled: 428 Confidence: 100.0% Identity: 23% PDB header:transport protein Chain: A: PDB Molecule:cation efflux system protein cusc; PDBTitle: outer membrane protein cusc Phyre2 PDB 1yc9 chain A Region: 53 - 477 Aligned: 411 Modelled: 411 Confidence: 100.0% Identity: 27% PDB header:membrane protein Chain: A: PDB Molecule:multidrug resistance protein; PDBTitle: the crystal structure of the outer membrane protein vcec from the2 bacterial pathogen vibrio cholerae at 1.8 resolution Phyre2 PDB 1tqq chain C Region: 66 - 473 Aligned: 393 Modelled: 394 Confidence: 100.0% Identity: 15% PDB header:transport protein Chain: C: PDB Molecule:outer membrane protein tolc; PDBTitle: structure of tolc in complex with hexamminecobalt Phyre2 PDB 1ek9 chain A Region: 66 - 473 Aligned: 393 Modelled: 391 Confidence: 100.0% Identity: 15% Fold: Outer membrane efflux proteins (OEP) Superfamily: Outer membrane efflux proteins (OEP) Family: Outer membrane efflux proteins (OEP) Phyre2 PDB 3fpp chain B Region: 183 - 264 Aligned: 82 Modelled: 82 Confidence: 43.6% Identity: 11% PDB header:membrane protein Chain: B: PDB Molecule:macrolide-specific efflux protein maca; PDBTitle: crystal structure of e.coli maca Phyre2 PDB 1urq chain C Region: 199 - 264 Aligned: 66 Modelled: 66 Confidence: 42.2% Identity: 15% PDB header:transport protein Chain: C: PDB Molecule:synaptosomal-associated protein 25; PDBTitle: crystal structure of neuronal q-snares in complex with2 r-snare motif of tomosyn Phyre2 PDB 2e76 chain D Region: 1 - 22 Aligned: 22 Modelled: 22 Confidence: 29.5% Identity: 23% PDB header:photosynthesis Chain: D: PDB Molecule:cytochrome b6-f complex iron-sulfur subunit; PDBTitle: crystal structure of the cytochrome b6f complex with tridecyl-2 stigmatellin (tds) from m.laminosus Phyre2 PDB 3b5n chain C Region: 204 - 264 Aligned: 61 Modelled: 61 Confidence: 23.9% Identity: 8% PDB header:membrane protein Chain: C: PDB Molecule:protein transport protein sec9; PDBTitle: structure of the yeast plasma membrane snare complex Phyre2 PDB 1v5r chain A domain 1 Region: 472 - 477 Aligned: 6 Modelled: 6 Confidence: 20.3% Identity: 50% Fold: N domain of copper amine oxidase-like Superfamily: GAS2 domain-like Family: GAS2 domain Phyre2 PDB 1nhl chain A Region: 213 - 264 Aligned: 52 Modelled: 52 Confidence: 14.6% Identity: 15% PDB header:protein transport Chain: A: PDB Molecule:synaptosomal-associated protein 23; PDBTitle: snap-23n structure Phyre2 PDB 1l4a chain C Region: 197 - 261 Aligned: 65 Modelled: 65 Confidence: 13.8% Identity: 18% PDB header:endocytosis/exocytosis Chain: C: PDB Molecule:s-snap25 fusion protein; PDBTitle: x-ray structure of the neuronal complexin/snare complex2 from the squid loligo pealei Phyre2 PDB 2l06 chain A Region: 216 - 269 Aligned: 54 Modelled: 54 Confidence: 12.3% Identity: 17% PDB header:protein binding Chain: A: PDB Molecule:phycobilisome lcm core-membrane linker polypeptide; PDBTitle: solution nmr structure of the pbs linker polypeptide domain (fragment2 254-400) of phycobilisome linker protein apce from synechocystis sp.3 pcc 6803. northeast structural genomics consortium target sgr209c Phyre2 PDB 3ohw chain B Region: 213 - 269 Aligned: 57 Modelled: 57 Confidence: 11.6% Identity: 12% PDB header:protein binding Chain: B: PDB Molecule:phycobilisome lcm core-membrane linker polypeptide; PDBTitle: x-ray structure of phycobilisome lcm core-membrane linker polypeptide2 (fragment 721-860) from synechocystis sp. pcc 6803, northeast3 structural genomics consortium target sgr209e Phyre2 PDB 2ijr chain A domain 1 Region: 14 - 58 Aligned: 45 Modelled: 44 Confidence: 10.6% Identity: 20% Fold: Api92-like Superfamily: Api92-like Family: Api92-like Phyre2 PDB 2l3w chain A Region: 216 - 269 Aligned: 54 Modelled: 54 Confidence: 10.5% Identity: 15% PDB header:photosynthesis Chain: A: PDB Molecule:phycobilisome rod linker polypeptide; PDBTitle: solution nmr structure of the pbs linker domain of phycobilisome rod2 linker polypeptide from synechococcus elongatus, northeast structural3 genomics consortium target snr168a Phyre2 PDB 3pru chain D Region: 219 - 269 Aligned: 51 Modelled: 51 Confidence: 10.3% Identity: 20% PDB header:photosynthesis Chain: D: PDB Molecule:phycobilisome 32.1 kda linker polypeptide, phycocyanin- PDBTitle: crystal structure of phycobilisome 32.1 kda linker polypeptide,2 phycocyanin-associated, rod 1 (fragment 14-158) from synechocystis3 sp. pcc 6803, northeast structural genomics consortium target sgr182a Phyre2 PDB 2ky4 chain A Region: 213 - 269 Aligned: 57 Modelled: 57 Confidence: 10.2% Identity: 12% PDB header:photosynthesis Chain: A: PDB Molecule:phycobilisome linker polypeptide; PDBTitle: solution nmr structure of the pbs linker domain of phycobilisome2 linker polypeptide from anabaena sp. northeast structural genomics3 consortium target nsr123e Phyre2 PDB 2f1m chain A Region: 396 - 468 Aligned: 73 Modelled: 73 Confidence: 9.3% Identity: 15% PDB header:transport protein Chain: A: PDB Molecule:acriflavine resistance protein a; PDBTitle: conformational flexibility in the multidrug efflux system protein acra Phyre2 PDB 3sog chain A Region: 359 - 473 Aligned: 113 Modelled: 115 Confidence: 8.1% Identity: 8% PDB header:structural protein Chain: A: PDB Molecule:amphiphysin; PDBTitle: crystal structure of the bar domain of human amphiphysin, isoform 1 Phyre2 1 d1wp1a_ 2 c3pikA_ 3 c1yc9A_ 4 c1tqqC_ 5 d1ek9a_ 6 c3fppB_ 7 c1urqC_ 8 c2e76D_ 9 c3b5nC_ 10 d1v5ra1 11 c1nhlA_ 12 c1l4aC_ 13 c2l06A_ 14 c3ohwB_ 15 d2ijra1 16 c2l3wA_ 17 c3pruD_ 18 c2ky4A_ 19 c2f1mA_ 20 c3sogA_ 21 22 23 24 25 26 27

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 d1wp1a_ 100.0 23 Fold:Outer membrane efflux proteins (OEP) Superfamily:Outer membrane efflux proteins (OEP) Family:Outer membrane efflux proteins (OEP) 2 c3pikA_ 100.0 23 PDB header:transport protein Chain: A: PDB Molecule:cation efflux system protein cusc; PDBTitle: outer membrane protein cusc 3 c1yc9A_ 100.0 27 PDB header:membrane protein Chain: A: PDB Molecule:multidrug resistance protein; PDBTitle: the crystal structure of the outer membrane protein vcec from the2 bacterial pathogen vibrio cholerae at 1.8 resolution 4 c1tqqC_ 100.0 15 PDB header:transport protein Chain: C: PDB Molecule:outer membrane protein tolc; PDBTitle: structure of tolc in complex with hexamminecobalt 5 d1ek9a_ 100.0 15 Fold:Outer membrane efflux proteins (OEP) Superfamily:Outer membrane efflux proteins (OEP) Family:Outer membrane efflux proteins (OEP) 6 c3fppB_ 43.6 11 PDB header:membrane protein Chain: B: PDB Molecule:macrolide-specific efflux protein maca; PDBTitle: crystal structure of e.coli maca 7 c1urqC_ 42.2 15 PDB header:transport protein Chain: C: PDB Molecule:synaptosomal-associated protein 25; PDBTitle: crystal structure of neuronal q-snares in complex with2 r-snare motif of tomosyn 8 c2e76D_ 29.5 23 PDB header:photosynthesis Chain: D: PDB Molecule:cytochrome b6-f complex iron-sulfur subunit; PDBTitle: crystal structure of the cytochrome b6f complex with tridecyl-2 stigmatellin (tds) from m.laminosus 9 c3b5nC_ 23.9 8 PDB header:membrane protein Chain: C: PDB Molecule:protein transport protein sec9; PDBTitle: structure of the yeast plasma membrane snare complex 10 d1v5ra1 20.3 50 Fold:N domain of copper amine oxidase-like Superfamily:GAS2 domain-like Family:GAS2 domain 11 c1nhlA_ 14.6 15 PDB header:protein transport Chain: A: PDB Molecule:synaptosomal-associated protein 23; PDBTitle: snap-23n structure 12 c1l4aC_ 13.8 18 PDB header:endocytosis/exocytosis Chain: C: PDB Molecule:s-snap25 fusion protein; PDBTitle: x-ray structure of the neuronal complexin/snare complex2 from the squid loligo pealei 13 c2l06A_ 12.3 17 PDB header:protein binding Chain: A: PDB Molecule:phycobilisome lcm core-membrane linker polypeptide; PDBTitle: solution nmr structure of the pbs linker polypeptide domain (fragment2 254-400) of phycobilisome linker protein apce from synechocystis sp.3 pcc 6803. northeast structural genomics consortium target sgr209c 14 c3ohwB_ 11.6 12 PDB header:protein binding Chain: B: PDB Molecule:phycobilisome lcm core-membrane linker polypeptide; PDBTitle: x-ray structure of phycobilisome lcm core-membrane linker polypeptide2 (fragment 721-860) from synechocystis sp. pcc 6803, northeast3 structural genomics consortium target sgr209e 15 d2ijra1 10.6 20 Fold:Api92-like Superfamily:Api92-like Family:Api92-like 16 c2l3wA_ 10.5 15 PDB header:photosynthesis Chain: A: PDB Molecule:phycobilisome rod linker polypeptide; PDBTitle: solution nmr structure of the pbs linker domain of phycobilisome rod2 linker polypeptide from synechococcus elongatus, northeast structural3 genomics consortium target snr168a 17 c3pruD_ 10.3 20 PDB header:photosynthesis Chain: D: PDB Molecule:phycobilisome 32.1 kda linker polypeptide, phycocyanin- PDBTitle: crystal structure of phycobilisome 32.1 kda linker polypeptide,2 phycocyanin-associated, rod 1 (fragment 14-158) from synechocystis3 sp. pcc 6803, northeast structural genomics consortium target sgr182a 18 c2ky4A_ 10.2 12 PDB header:photosynthesis Chain: A: PDB Molecule:phycobilisome linker polypeptide; PDBTitle: solution nmr structure of the pbs linker domain of phycobilisome2 linker polypeptide from anabaena sp. northeast structural genomics3 consortium target nsr123e 19 c2f1mA_ 9.3 15 PDB header:transport protein Chain: A: PDB Molecule:acriflavine resistance protein a; PDBTitle: conformational flexibility in the multidrug efflux system protein acra 20 c3sogA_ 8.1 8 PDB header:structural protein Chain: A: PDB Molecule:amphiphysin; PDBTitle: crystal structure of the bar domain of human amphiphysin, isoform 1 21 c2v7sA_ not modelled 7.2 13 PDB header:unknown function Chain: A: PDB Molecule:probable conserved lipoprotein lppa; PDBTitle: crystal structure of the putative lipoprotein lppa from2 mycobacterium tuberculosis 22 c3brzA_ not modelled 7.0 13 PDB header:transport protein Chain: A: PDB Molecule:todx; PDBTitle: crystal structure of the pseudomonas putida toluene2 transporter todx 23 d1d8ja_ not modelled 6.3 42 Fold:DNA/RNA-binding 3-helical bundle Superfamily:"Winged helix" DNA-binding domain Family:The central core domain of TFIIE beta 24 c1yd7A_ not modelled 5.7 14 PDB header:oxidoreductase Chain: A: PDB Molecule:2-keto acid:ferredoxin oxidoreductase subunit PDBTitle: conserved hypothetical protein pfu-1647980-001 from2 pyrococcus furiosus 25 c2dq3A_ not modelled 5.5 4 PDB header:ligase Chain: A: PDB Molecule:seryl-trna synthetase; PDBTitle: crystal structure of aq_298 26 c3zqbB_ not modelled 5.4 14 PDB header:cell invasion Chain: B: PDB Molecule:protein prgi, cell invasion protein sipd; PDBTitle: prgi-sipd from salmonella typhimurium 27 c2wasA_ not modelled 5.3 14 PDB header:transferase Chain: A: PDB Molecule:3-oxoacyl-[acyl-carrier-protein] synthase; PDBTitle: structure of the fungal type i fas ppt domain

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0