Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	A	N	L	L	N	K	F	I	M	T	R	I	L	A	A	I	T	L	L	L	S	I	V	L	T	I	L	V	T	I	F	C	S	V	P	I	I	I	A	G	I	V	K	L	L	L	P	V	P	V	I	W	R	K	V	S	R	F	C
Secondary structure
SS confidence
Disorder		?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		D	F	M	M	Y	C	W	C	E	G	L	A	V	L	L	H	L	N	P	H	L	Q	W	E	V	H	G	L	E	G	L	S	K	K	N	W	Y	L	L	I	C	N	H	R	S	W	A	D	I	V	V	L	C	V	L	F	R	K	H	I
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		P	M	N	K	Y	F	L	K	Q	Q	L	A	W	V	P	F	L	G	L	A	C	W	S	L	D	M	P	F	M	K	R	Y	S	R	A	Y	L	L	R	H	P	E	R	R	G	K	D	V	E	T	T	R	R	S	C	E	K	F	R	L
Secondary structure
SS confidence
Disorder																																			?	?	?	?	?		?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		H	P	T	T	I	V	N	F	V	E	G	S	R	F	T	Q	E	K	H	Q	Q	T	H	S	T	F	Q	N	L	L	P	P	K	A	A	G	I	A	M	A	L	N	V	L	G	K	Q	F	D	K	L	L	N	V	T	L	C	Y	P	D
Secondary structure
SS confidence
Disorder													?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		N	N	R	Q	P	F	F	D	M	L	S	G	K	L	T	R	I	V	V	H	V	D	L	Q	P	I	A	D	E	L	H	G	D	Y	I	N	D	K	S	F	K	R	H	F	Q	Q	W	L	N	S	L	W	Q	E	K	D	R	L	L	T
Secondary structure
SS confidence
Disorder																																			?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310
Sequence		S	L	M	S	S	Q	R	Q	N	K
Secondary structure
SS confidence
Disorder						?	?	?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 1iuq chain A Region: 89 - 307 Aligned: 212 Modelled: 219 Confidence: 98.4% Identity: 14% Fold: Glycerol-3-phosphate (1)-acyltransferase Superfamily: Glycerol-3-phosphate (1)-acyltransferase Family: Glycerol-3-phosphate (1)-acyltransferase Phyre2 PDB 3jur chain A Region: 167 - 192 Aligned: 26 Modelled: 26 Confidence: 38.8% Identity: 19% PDB header:hydrolase Chain: A: PDB Molecule:exo-poly-alpha-d-galacturonosidase; PDBTitle: the crystal structure of a hyperthermoactive exopolygalacturonase from2 thermotoga maritima Phyre2 PDB 2pyh chain B Region: 167 - 192 Aligned: 26 Modelled: 26 Confidence: 23.7% Identity: 12% PDB header:isomerase Chain: B: PDB Molecule:poly(beta-d-mannuronate) c5 epimerase 4; PDBTitle: azotobacter vinelandii mannuronan c-5 epimerase alge4 a-module2 complexed with mannuronan trisaccharide Phyre2 PDB 3eqn chain B Region: 167 - 192 Aligned: 26 Modelled: 26 Confidence: 23.4% Identity: 27% PDB header:hydrolase Chain: B: PDB Molecule:glucan 1,3-beta-glucosidase; PDBTitle: crystal structure of beta-1,3-glucanase from phanerochaete2 chrysosporium (lam55a) Phyre2 PDB 3hkx chain A Region: 165 - 192 Aligned: 28 Modelled: 28 Confidence: 21.5% Identity: 4% PDB header:hydrolase Chain: A: PDB Molecule:amidase; PDBTitle: crystal structure analysis of an amidase from nesterenkonia sp. Phyre2 PDB 2vhi chain G Region: 156 - 190 Aligned: 35 Modelled: 35 Confidence: 13.2% Identity: 11% PDB header:hydrolase Chain: G: PDB Molecule:cg3027-pa; PDBTitle: crystal structure of a pyrimidine degrading enzyme from2 drosophila melanogaster Phyre2 PDB 1uf5 chain A Region: 165 - 192 Aligned: 28 Modelled: 28 Confidence: 11.6% Identity: 14% Fold: Carbon-nitrogen hydrolase Superfamily: Carbon-nitrogen hydrolase Family: Carbamilase Phyre2 PDB 3n2s chain D Region: 224 - 310 Aligned: 66 Modelled: 87 Confidence: 11.0% Identity: 14% PDB header:oxidoreductase Chain: D: PDB Molecule:nadph-dependent nitro/flavin reductase; PDBTitle: structure of nfra1 nitroreductase from b. subtilis Phyre2 PDB 1j31 chain A Region: 165 - 190 Aligned: 26 Modelled: 26 Confidence: 11.0% Identity: 12% Fold: Carbon-nitrogen hydrolase Superfamily: Carbon-nitrogen hydrolase Family: Carbamilase Phyre2 PDB 2w1v chain A Region: 165 - 192 Aligned: 28 Modelled: 28 Confidence: 10.3% Identity: 11% PDB header:hydrolase Chain: A: PDB Molecule:nitrilase homolog 2; PDBTitle: crystal structure of mouse nitrilase-2 at 1.4a resolution Phyre2 PDB 1ems chain A domain 2 Region: 165 - 192 Aligned: 28 Modelled: 28 Confidence: 9.9% Identity: 14% Fold: Carbon-nitrogen hydrolase Superfamily: Carbon-nitrogen hydrolase Family: Nitrilase Phyre2 PDB 3n05 chain B Region: 165 - 190 Aligned: 26 Modelled: 26 Confidence: 8.8% Identity: 19% PDB header:ligase Chain: B: PDB Molecule:nh(3)-dependent nad(+) synthetase; PDBTitle: crystal structure of nh3-dependent nad+ synthetase from streptomyces2 avermitilis Phyre2 PDB 3ilv chain A Region: 165 - 190 Aligned: 26 Modelled: 26 Confidence: 8.8% Identity: 15% PDB header:ligase Chain: A: PDB Molecule:glutamine-dependent nad(+) synthetase; PDBTitle: crystal structure of a glutamine-dependent nad(+) synthetase2 from cytophaga hutchinsonii Phyre2 PDB 2inu chain C Region: 165 - 192 Aligned: 28 Modelled: 28 Confidence: 8.3% Identity: 7% PDB header:lyase Chain: C: PDB Molecule:insulin fructotransferase; PDBTitle: crystal structure of insulin fructotransferase in the absence of2 substrate Phyre2 PDB 2e2k chain C Region: 160 - 190 Aligned: 31 Modelled: 31 Confidence: 8.2% Identity: 13% PDB header:hydrolase Chain: C: PDB Molecule:formamidase; PDBTitle: helicobacter pylori formamidase amif contains a fine-tuned cysteine-2 glutamate-lysine catalytic triad Phyre2 PDB 2cve chain A domain 2 Region: 189 - 216 Aligned: 15 Modelled: 28 Confidence: 7.2% Identity: 47% Fold: Ferredoxin-like Superfamily: EF-G C-terminal domain-like Family: YigZ C-terminal domain-like Phyre2 PDB 3dla chain D Region: 165 - 190 Aligned: 26 Modelled: 26 Confidence: 6.6% Identity: 8% PDB header:ligase Chain: D: PDB Molecule:glutamine-dependent nad(+) synthetase; PDBTitle: x-ray crystal structure of glutamine-dependent nad+ synthetase from2 mycobacterium tuberculosis bound to naad+ and don Phyre2 PDB 1rmg chain A Region: 167 - 192 Aligned: 24 Modelled: 26 Confidence: 6.5% Identity: 8% Fold: Single-stranded right-handed beta-helix Superfamily: Pectin lyase-like Family: Galacturonase Phyre2 PDB 1avo chain A Region: 277 - 303 Aligned: 27 Modelled: 27 Confidence: 6.4% Identity: 15% PDB header:proteasome activator Chain: A: PDB Molecule:11s regulator; PDBTitle: proteasome activator reg(alpha) Phyre2 PDB 1czf chain B Region: 167 - 192 Aligned: 26 Modelled: 26 Confidence: 6.0% Identity: 8% PDB header:hydrolase Chain: B: PDB Molecule:polygalacturonase ii; PDBTitle: endo-polygalacturonase ii from aspergillus niger Phyre2 1 d1iuqa_ 2 c3jurA_ 3 c2pyhB_ 4 c3eqnB_ 5 c3hkxA_ 6 c2vhiG_ 7 d1uf5a_ 8 c3n2sD_ 9 d1j31a_ 10 c2w1vA_ 11 d1emsa2 12 c3n05B_ 13 c3ilvA_ 14 c2inuC_ 15 c2e2kC_ 16 d2cvea2 17 c3dlaD_ 18 d1rmga_ 19 c1avoA_ 20 c1czfB_ 21 22 23 24 25 26 27 28

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 d1iuqa_ 98.4 14 Fold:Glycerol-3-phosphate (1)-acyltransferase Superfamily:Glycerol-3-phosphate (1)-acyltransferase Family:Glycerol-3-phosphate (1)-acyltransferase 2 c3jurA_ 38.8 19 PDB header:hydrolase Chain: A: PDB Molecule:exo-poly-alpha-d-galacturonosidase; PDBTitle: the crystal structure of a hyperthermoactive exopolygalacturonase from2 thermotoga maritima 3 c2pyhB_ 23.7 12 PDB header:isomerase Chain: B: PDB Molecule:poly(beta-d-mannuronate) c5 epimerase 4; PDBTitle: azotobacter vinelandii mannuronan c-5 epimerase alge4 a-module2 complexed with mannuronan trisaccharide 4 c3eqnB_ 23.4 27 PDB header:hydrolase Chain: B: PDB Molecule:glucan 1,3-beta-glucosidase; PDBTitle: crystal structure of beta-1,3-glucanase from phanerochaete2 chrysosporium (lam55a) 5 c3hkxA_ 21.5 4 PDB header:hydrolase Chain: A: PDB Molecule:amidase; PDBTitle: crystal structure analysis of an amidase from nesterenkonia sp. 6 c2vhiG_ 13.2 11 PDB header:hydrolase Chain: G: PDB Molecule:cg3027-pa; PDBTitle: crystal structure of a pyrimidine degrading enzyme from2 drosophila melanogaster 7 d1uf5a_ 11.6 14 Fold:Carbon-nitrogen hydrolase Superfamily:Carbon-nitrogen hydrolase Family:Carbamilase 8 c3n2sD_ 11.0 14 PDB header:oxidoreductase Chain: D: PDB Molecule:nadph-dependent nitro/flavin reductase; PDBTitle: structure of nfra1 nitroreductase from b. subtilis 9 d1j31a_ 11.0 12 Fold:Carbon-nitrogen hydrolase Superfamily:Carbon-nitrogen hydrolase Family:Carbamilase 10 c2w1vA_ 10.3 11 PDB header:hydrolase Chain: A: PDB Molecule:nitrilase homolog 2; PDBTitle: crystal structure of mouse nitrilase-2 at 1.4a resolution 11 d1emsa2 9.9 14 Fold:Carbon-nitrogen hydrolase Superfamily:Carbon-nitrogen hydrolase Family:Nitrilase 12 c3n05B_ 8.8 19 PDB header:ligase Chain: B: PDB Molecule:nh(3)-dependent nad(+) synthetase; PDBTitle: crystal structure of nh3-dependent nad+ synthetase from streptomyces2 avermitilis 13 c3ilvA_ 8.8 15 PDB header:ligase Chain: A: PDB Molecule:glutamine-dependent nad(+) synthetase; PDBTitle: crystal structure of a glutamine-dependent nad(+) synthetase2 from cytophaga hutchinsonii 14 c2inuC_ 8.3 7 PDB header:lyase Chain: C: PDB Molecule:insulin fructotransferase; PDBTitle: crystal structure of insulin fructotransferase in the absence of2 substrate 15 c2e2kC_ 8.2 13 PDB header:hydrolase Chain: C: PDB Molecule:formamidase; PDBTitle: helicobacter pylori formamidase amif contains a fine-tuned cysteine-2 glutamate-lysine catalytic triad 16 d2cvea2 7.2 47 Fold:Ferredoxin-like Superfamily:EF-G C-terminal domain-like Family:YigZ C-terminal domain-like 17 c3dlaD_ 6.6 8 PDB header:ligase Chain: D: PDB Molecule:glutamine-dependent nad(+) synthetase; PDBTitle: x-ray crystal structure of glutamine-dependent nad+ synthetase from2 mycobacterium tuberculosis bound to naad+ and don 18 d1rmga_ 6.5 8 Fold:Single-stranded right-handed beta-helix Superfamily:Pectin lyase-like Family:Galacturonase 19 c1avoA_ 6.4 15 PDB header:proteasome activator Chain: A: PDB Molecule:11s regulator; PDBTitle: proteasome activator reg(alpha) 20 c1czfB_ 6.0 8 PDB header:hydrolase Chain: B: PDB Molecule:polygalacturonase ii; PDBTitle: endo-polygalacturonase ii from aspergillus niger 21 d1czfa_ not modelled 6.0 8 Fold:Single-stranded right-handed beta-helix Superfamily:Pectin lyase-like Family:Galacturonase 22 c2plqA_ not modelled 6.0 9 PDB header:hydrolase Chain: A: PDB Molecule:aliphatic amidase; PDBTitle: crystal structure of the amidase from geobacillus pallidus rapc8 23 d2hthb1 not modelled 5.9 25 Fold:PH domain-like barrel Superfamily:PH domain-like Family:VPS36 N-terminal domain-like 24 c2h3nD_ not modelled 5.7 33 PDB header:immune system Chain: D: PDB Molecule:ig lambda-5; PDBTitle: crystal structure of a surrogate light chain (lambda5 and2 vpreb) homodimer 25 d2a4aa1 not modelled 5.7 22 Fold:TIM beta/alpha-barrel Superfamily:Aldolase Family:Class I aldolase 26 c3hykB_ not modelled 5.3 5 PDB header:transferase Chain: B: PDB Molecule:holo-[acyl-carrier-protein] synthase; PDBTitle: 2.31 angstrom resolution crystal structure of a holo-(acyl-carrier-2 protein) synthase from bacillus anthracis str. ames in complex with3 coa (3',5'-adp) 27 d1f89a_ not modelled 5.2 14 Fold:Carbon-nitrogen hydrolase Superfamily:Carbon-nitrogen hydrolase Family:Nitrilase 28 c3efdK_ not modelled 5.2 10 PDB header:immune system Chain: K: PDB Molecule:kcsa; PDBTitle: the crystal structure of the cytoplasmic domain of kcsa

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0