Email l.a.kelley@imperial.ac.uk Description P37640 Date Thu Jan 5 11:56:12 GMT 2012 Unique Job ID 4cbb49dd52d4dfc5

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 c1zn2A_ 100.0 14 PDB header:transcription regulator Chain: A: PDB Molecule:response regulatory protein; PDBTitle: low resolution structure of response regulator styr 2 c3c3wB_ 100.0 19 PDB header:transcription Chain: B: PDB Molecule:two component transcriptional regulatory protein devr; PDBTitle: crystal structure of the mycobacterium tuberculosis hypoxic response2 regulator dosr 3 c1rnlA_ 100.0 21 PDB header:signal transduction protein Chain: A: PDB Molecule:nitrate/nitrite response regulator protein narl; PDBTitle: the nitrate/nitrite response regulator protein narl from narl 4 c3klnC_ 100.0 20 PDB header:transcription Chain: C: PDB Molecule:transcriptional regulator, luxr family; PDBTitle: vibrio cholerae vpst 5 c1kgsA_ 99.9 11 PDB header:dna binding protein Chain: A: PDB Molecule:dna binding response regulator d; PDBTitle: crystal structure at 1.50 a of an ompr/phob homolog from thermotoga2 maritima 6 c3q9sA_ 99.9 15 PDB header:dna binding protein Chain: A: PDB Molecule:dna-binding response regulator; PDBTitle: crystal structure of rra(1-215) from deinococcus radiodurans 7 c3r0jA_ 99.9 13 PDB header:dna binding protein Chain: A: PDB Molecule:possible two component system response transcriptional PDBTitle: structure of phop from mycobacterium tuberculosis 8 c1ys7B_ 99.9 13 PDB header:transcription regulator Chain: B: PDB Molecule:transcriptional regulatory protein prra; PDBTitle: crystal structure of the response regulator protein prra comlexed with2 mg2+ 9 c2gwrA_ 99.9 13 PDB header:signaling protein Chain: A: PDB Molecule:dna-binding response regulator mtra; PDBTitle: crystal structure of the response regulator protein mtra from2 mycobacterium tuberculosis 10 c2hqrA_ 99.9 10 PDB header:signaling protein Chain: A: PDB Molecule:putative transcriptional regulator; PDBTitle: structure of a atypical orphan response regulator protein revealed a2 new phosphorylation-independent regulatory mechanism 11 c2oqrA_ 99.9 11 PDB header:transcription,signaling protein Chain: A: PDB Molecule:sensory transduction protein regx3; PDBTitle: the structure of the response regulator regx3 from mycobacterium2 tuberculosis 12 c1p2fA_ 99.8 15 PDB header:transcription Chain: A: PDB Molecule:response regulator; PDBTitle: crystal structure analysis of response regulator drrb, a2 thermotoga maritima ompr/phob homolog 13 d1s8na_ 99.8 10 Fold:Flavodoxin-like Superfamily:CheY-like Family:CheY-related 14 c3cz5B_ 99.7 14 PDB header:transcription regulator Chain: B: PDB Molecule:two-component response regulator, luxr family; PDBTitle: crystal structure of two-component response regulator, luxr family,2 from aurantimonas sp. si85-9a1 15 c3eulB_ 99.7 15 PDB header:transcription Chain: B: PDB Molecule:possible nitrate/nitrite response transcriptional PDBTitle: structure of the signal receiver domain of the putative2 response regulator narl from mycobacterium tuberculosis 16 c2ayxA_ 99.7 15 PDB header:transferase Chain: A: PDB Molecule:sensor kinase protein rcsc; PDBTitle: solution structure of the e.coli rcsc c-terminus (residues2 700-949) containing linker region and phosphoreceiver3 domain 17 d1a04a2 99.7 17 Fold:Flavodoxin-like Superfamily:CheY-like Family:CheY-related 18 c3eq2A_ 99.7 12 PDB header:signaling protein Chain: A: PDB Molecule:probable two-component response regulator; PDBTitle: structure of hexagonal crystal form of pseudomonas2 aeruginosa rssb 19 c3b2nA_ 99.7 15 PDB header:transcription Chain: A: PDB Molecule:uncharacterized protein q99uf4; PDBTitle: crystal structure of dna-binding response regulator, luxr family, from2 staphylococcus aureus 20 c1ny5A_ 99.7 16 PDB header:transcription Chain: A: PDB Molecule:transcriptional regulator (ntrc family); PDBTitle: crystal structure of sigm54 activator (aaa+ atpase) in the inactive2 state 21 c3breA_ not modelled 99.7 9 PDB header:signaling protein Chain: A: PDB Molecule:probable two-component response regulator; PDBTitle: crystal structure of p.aeruginosa pa3702 22 d1dz3a_ not modelled 99.7 11 Fold:Flavodoxin-like Superfamily:CheY-like Family:CheY-related 23 d1ny5a1 not modelled 99.7 16 Fold:Flavodoxin-like Superfamily:CheY-like Family:CheY-related 24 c1a2oB_ not modelled 99.7 11 PDB header:bacterial chemotaxis Chain: B: PDB Molecule:cheb methylesterase; PDBTitle: structural basis for methylesterase cheb regulation by a2 phosphorylation-activated domain 25 d1kgsa2 not modelled 99.7 9 Fold:Flavodoxin-like Superfamily:CheY-like Family:CheY-related 26 c3cu5B_ not modelled 99.7 14 PDB header:transcription regulator Chain: B: PDB Molecule:two component transcriptional regulator, arac family; PDBTitle: crystal structure of a two component transcriptional regulator arac2 from clostridium phytofermentans isdg 27 c3rqiA_ not modelled 99.7 13 PDB header:transcription Chain: A: PDB Molecule:response regulator protein; PDBTitle: crystal structure of a response regulator protein from burkholderia2 pseudomallei with a phosphorylated aspartic acid, calcium ion and3 citrate 28 c1w25B_ not modelled 99.7 9 PDB header:signaling protein Chain: B: PDB Molecule:stalked-cell differentiation controlling protein; PDBTitle: response regulator pled in complex with c-digmp 29 c3i5aA_ not modelled 99.7 6 PDB header:signaling protein Chain: A: PDB Molecule:response regulator/ggdef domain protein; PDBTitle: crystal structure of full-length wpsr from pseudomonas syringae 30 c2qv0A_ not modelled 99.7 6 PDB header:transcription Chain: A: PDB Molecule:protein mrke; PDBTitle: crystal structure of the response regulatory domain of2 protein mrke from klebsiella pneumoniae 31 d1ys7a2 not modelled 99.7 9 Fold:Flavodoxin-like Superfamily:CheY-like Family:CheY-related 32 d1jbea_ not modelled 99.7 14 Fold:Flavodoxin-like Superfamily:CheY-like Family:CheY-related 33 d1a2oa1 not modelled 99.7 9 Fold:Flavodoxin-like Superfamily:CheY-like Family:CheY-related 34 d1peya_ not modelled 99.7 17 Fold:Flavodoxin-like Superfamily:CheY-like Family:CheY-related 35 d1u0sy_ not modelled 99.7 11 Fold:Flavodoxin-like Superfamily:CheY-like Family:CheY-related 36 d2ayxa1 not modelled 99.7 15 Fold:Flavodoxin-like Superfamily:CheY-like Family:CheY-related 37 c3hdgE_ not modelled 99.7 8 PDB header:structural genomics, unknown function Chain: E: PDB Molecule:uncharacterized protein; PDBTitle: crystal structure of the n-terminal domain of an2 uncharacterized protein (ws1339) from wolinella3 succinogenes 38 d1dbwa_ not modelled 99.6 8 Fold:Flavodoxin-like Superfamily:CheY-like Family:CheY-related 39 c3dzdA_ not modelled 99.6 12 PDB header:transcription regulator Chain: A: PDB Molecule:transcriptional regulator (ntrc family); PDBTitle: crystal structure of sigma54 activator ntrc4 in the inactive2 state 40 d2a9pa1 not modelled 99.6 11 Fold:Flavodoxin-like Superfamily:CheY-like Family:CheY-related 41 c3t8yA_ not modelled 99.6 9 PDB header:hydrolase Chain: A: PDB Molecule:chemotaxis response regulator protein-glutamate PDBTitle: crystal structure of the response regulator domain of thermotoga2 maritima cheb 42 c3sztB_ not modelled 99.6 33 PDB header:transcription Chain: B: PDB Molecule:quorum-sensing control repressor; PDBTitle: quorum sensing control repressor, qscr, bound to n-3-oxo-dodecanoyl-l-2 homoserine lactone 43 c3f6cB_ not modelled 99.6 13 PDB header:dna binding protein Chain: B: PDB Molecule:positive transcription regulator evga; PDBTitle: crystal structure of n-terminal domain of positive transcription2 regulator evga from escherichia coli 44 c2rjnA_ not modelled 99.6 10 PDB header:hydrolase Chain: A: PDB Molecule:response regulator receiver:metal-dependent PDBTitle: crystal structure of an uncharacterized protein q2bku2 from2 neptuniibacter caesariensis 45 d1mvoa_ not modelled 99.6 13 Fold:Flavodoxin-like Superfamily:CheY-like Family:CheY-related 46 d1krwa_ not modelled 99.6 14 Fold:Flavodoxin-like Superfamily:CheY-like Family:CheY-related 47 c2zwmA_ not modelled 99.6 10 PDB header:transcription Chain: A: PDB Molecule:transcriptional regulatory protein yycf; PDBTitle: crystal structure of yycf receiver domain from bacillus2 subtilis 48 c3cnbC_ not modelled 99.6 11 PDB header:dna binding protein Chain: C: PDB Molecule:dna-binding response regulator, merr family; PDBTitle: crystal structure of signal receiver domain of dna binding response2 regulator protein (merr) from colwellia psychrerythraea 34h 49 d1qkka_ not modelled 99.6 13 Fold:Flavodoxin-like Superfamily:CheY-like Family:CheY-related 50 d2pl1a1 not modelled 99.6 14 Fold:Flavodoxin-like Superfamily:CheY-like Family:CheY-related 51 c2qr3A_ not modelled 99.6 14 PDB header:transcription Chain: A: PDB Molecule:two-component system response regulator; PDBTitle: crystal structure of the n-terminal signal receiver domain of two-2 component system response regulator from bacteroides fragilis 52 c1h0mD_ not modelled 99.6 17 PDB header:transcription/dna Chain: D: PDB Molecule:transcriptional activator protein trar; PDBTitle: three-dimensional structure of the quorum sensing protein2 trar bound to its autoinducer and to its target dna 53 c2jrlA_ not modelled 99.6 14 PDB header:transcription Chain: A: PDB Molecule:transcriptional regulator (ntrc family); PDBTitle: solution structure of the beryllofluoride-activated ntrc4 receiver2 domain dimer 54 d1yioa2 not modelled 99.6 9 Fold:Flavodoxin-like Superfamily:CheY-like Family:CheY-related 55 c2q0oA_ not modelled 99.6 17 PDB header:transcription Chain: A: PDB Molecule:probable transcriptional activator protein trar; PDBTitle: crystal structure of an anti-activation complex in bacterial quorum2 sensing 56 c2qvgA_ not modelled 99.6 10 PDB header:transferase Chain: A: PDB Molecule:two component response regulator; PDBTitle: the crystal structure of a two-component response regulator2 from legionella pneumophila 57 c3nhzA_ not modelled 99.6 11 PDB header:dna binding protein Chain: A: PDB Molecule:two component system transcriptional regulator mtra; PDBTitle: structure of n-terminal domain of mtra 58 c3hv2B_ not modelled 99.6 17 PDB header:signaling protein Chain: B: PDB Molecule:response regulator/hd domain protein; PDBTitle: crystal structure of signal receiver domain of hd domain-2 containing protein from pseudomonas fluorescens pf-5 59 c3khtA_ not modelled 99.6 8 PDB header:signaling protein Chain: A: PDB Molecule:response regulator; PDBTitle: crystal structure of response regulator from hahella chejuensis 60 c3cfyA_ not modelled 99.6 10 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:putative luxo repressor protein; PDBTitle: crystal structure of signal receiver domain of putative luxo2 repressor protein from vibrio parahaemolyticus 61 c3qp5C_ not modelled 99.6 36 PDB header:transcription Chain: C: PDB Molecule:cvir transcriptional regulator; PDBTitle: crystal structure of cvir bound to antagonist chlorolactone (cl) 62 c3hebB_ not modelled 99.6 14 PDB header:transcription regulator Chain: B: PDB Molecule:response regulator receiver domain protein (chey); PDBTitle: crystal structure of response regulator receiver domain from2 rhodospirillum rubrum 63 d1w25a1 not modelled 99.6 9 Fold:Flavodoxin-like Superfamily:CheY-like Family:CheY-related 64 c3gt7A_ not modelled 99.6 13 PDB header:hydrolase Chain: A: PDB Molecule:sensor protein; PDBTitle: crystal structure of signal receiver domain of signal2 transduction histidine kinase from syntrophus3 aciditrophicus 65 c3jteA_ not modelled 99.6 16 PDB header:protein binding Chain: A: PDB Molecule:response regulator receiver protein; PDBTitle: crystal structure of response regulator receiver domain2 protein from clostridium thermocellum 66 d1zh2a1 not modelled 99.6 9 Fold:Flavodoxin-like Superfamily:CheY-like Family:CheY-related 67 d1k66a_ not modelled 99.6 14 Fold:Flavodoxin-like Superfamily:CheY-like Family:CheY-related 68 c2qzjC_ not modelled 99.6 15 PDB header:transcription Chain: C: PDB Molecule:two-component response regulator; PDBTitle: crystal structure of a two-component response regulator from2 clostridium difficile 69 d1p6qa_ not modelled 99.6 16 Fold:Flavodoxin-like Superfamily:CheY-like Family:CheY-related 70 c3t6kB_ not modelled 99.6 10 PDB header:signaling protein Chain: B: PDB Molecule:response regulator receiver; PDBTitle: crystal structure of a hypothetical response regulator (caur_3799)2 from chloroflexus aurantiacus j-10-fl at 1.86 a resolution 71 c3crnA_ not modelled 99.6 16 PDB header:signaling protein Chain: A: PDB Molecule:response regulator receiver domain protein, chey-like; PDBTitle: crystal structure of response regulator receiver domain protein (chey-2 like) from methanospirillum hungatei jf-1 72 c3a0rB_ not modelled 99.6 18 PDB header:transferase Chain: B: PDB Molecule:response regulator; PDBTitle: crystal structure of histidine kinase thka (tm1359) in complex with2 response regulator protein trra (tm1360) 73 d1zesa1 not modelled 99.6 10 Fold:Flavodoxin-like Superfamily:CheY-like Family:CheY-related 74 c3h1gA_ not modelled 99.6 16 PDB header:signaling protein Chain: A: PDB Molecule:chemotaxis protein chey homolog; PDBTitle: crystal structure of chey mutant t84a of helicobacter pylori 75 d1zgza1 not modelled 99.6 11 Fold:Flavodoxin-like Superfamily:CheY-like Family:CheY-related 76 c2jk1A_ not modelled 99.6 17 PDB header:dna-binding Chain: A: PDB Molecule:hydrogenase transcriptional regulatory protein hupr1; PDBTitle: crystal structure of the wild-type hupr receiver domain 77 d1i3ca_ not modelled 99.5 11 Fold:Flavodoxin-like Superfamily:CheY-like Family:CheY-related 78 d1xhfa1 not modelled 99.5 13 Fold:Flavodoxin-like Superfamily:CheY-like Family:CheY-related 79 c3m6mF_ not modelled 99.5 12 PDB header:lyase/transferase Chain: F: PDB Molecule:sensory/regulatory protein rpfc; PDBTitle: crystal structure of rpff complexed with rec domain of rpfc 80 c3lteH_ not modelled 99.5 10 PDB header:transcription Chain: H: PDB Molecule:response regulator; PDBTitle: crystal structure of response regulator (signal receiver domain) from2 bermanella marisrubri 81 c3ilhA_ not modelled 99.5 15 PDB header:transcription regulator Chain: A: PDB Molecule:two component response regulator; PDBTitle: crystal structure of two component response regulator from cytophaga2 hutchinsonii 82 c2zayA_ not modelled 99.5 11 PDB header:signaling protein Chain: A: PDB Molecule:response regulator receiver protein; PDBTitle: crystal structure of response regulator from desulfuromonas2 acetoxidans 83 d1heya_ not modelled 99.5 13 Fold:Flavodoxin-like Superfamily:CheY-like Family:CheY-related 84 c3snkA_ not modelled 99.5 12 PDB header:signaling protein Chain: A: PDB Molecule:response regulator chey-like protein; PDBTitle: crystal structure of a response regulator chey-like protein (mll6475)2 from mesorhizobium loti at 2.02 a resolution 85 c3kcnA_ not modelled 99.5 13 PDB header:lyase Chain: A: PDB Molecule:adenylate cyclase homolog; PDBTitle: the crystal structure of adenylate cyclase from2 rhodopirellula baltica 86 c3c3mA_ not modelled 99.5 14 PDB header:signaling protein Chain: A: PDB Molecule:response regulator receiver protein; PDBTitle: crystal structure of the n-terminal domain of response regulator2 receiver protein from methanoculleus marisnigri jr1 87 c3i42A_ not modelled 99.5 11 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:response regulator receiver domain protein (chey- PDBTitle: structure of response regulator receiver domain (chey-like)2 from methylobacillus flagellatus 88 c3cg4A_ not modelled 99.5 11 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:response regulator receiver domain protein (chey-like); PDBTitle: crystal structure of response regulator receiver domain protein (chey-2 like) from methanospirillum hungatei jf-1 89 c2qxyB_ not modelled 99.5 17 PDB header:transcription Chain: B: PDB Molecule:response regulator; PDBTitle: crystal structure of a response regulator from thermotoga2 maritima 90 d1mb3a_ not modelled 99.5 7 Fold:Flavodoxin-like Superfamily:CheY-like Family:CheY-related 91 d2r25b1 not modelled 99.5 8 Fold:Flavodoxin-like Superfamily:CheY-like Family:CheY-related 92 c1zljE_ not modelled 99.5 30 PDB header:transcription Chain: E: PDB Molecule:dormancy survival regulator; PDBTitle: crystal structure of the mycobacterium tuberculosis hypoxic2 response regulator dosr c-terminal domain 93 d1p2fa2 not modelled 99.5 13 Fold:Flavodoxin-like Superfamily:CheY-like Family:CheY-related 94 c3hzhA_ not modelled 99.5 8 PDB header:signaling protein Chain: A: PDB Molecule:chemotaxis response regulator (chey-3); PDBTitle: crystal structure of the chex-chey-bef3-mg+2 complex from2 borrelia burgdorferi 95 c3c97A_ not modelled 99.5 7 PDB header:signaling protein, transferase Chain: A: PDB Molecule:signal transduction histidine kinase; PDBTitle: crystal structure of the response regulator receiver domain2 of a signal transduction histidine kinase from aspergillus3 oryzae 96 c2rdmB_ not modelled 99.5 14 PDB header:structural genomics, unknown function Chain: B: PDB Molecule:response regulator receiver protein; PDBTitle: crystal structure of response regulator receiver protein from2 sinorhizobium medicae wsm419 97 d1p4wa_ not modelled 99.5 28 Fold:DNA/RNA-binding 3-helical bundle Superfamily:C-terminal effector domain of the bipartite response regulators Family:GerE-like (LuxR/UhpA family of transcriptional regulators) 98 c3grcD_ not modelled 99.5 9 PDB header:transferase Chain: D: PDB Molecule:sensor protein, kinase; PDBTitle: crystal structure of a sensor protein from polaromonas sp.2 js666 99 c3cloC_ not modelled 99.5 37 PDB header:transcription regulator Chain: C: PDB Molecule:transcriptional regulator; PDBTitle: crystal structure of putative transcriptional regulator containing a2 luxr dna binding domain (np_811094.1) from bacteroides3 thetaiotaomicron vpi-5482 at 2.04 a resolution 100 c2qsjB_ not modelled 99.5 19 PDB header:transcription Chain: B: PDB Molecule:dna-binding response regulator, luxr family; PDBTitle: crystal structure of a luxr family dna-binding response2 regulator from silicibacter pomeroyi 101 d2b4aa1 not modelled 99.5 14 Fold:Flavodoxin-like Superfamily:CheY-like Family:CheY-related 102 c3mmnA_ not modelled 99.5 10 PDB header:transferase Chain: A: PDB Molecule:histidine kinase homolog; PDBTitle: crystal structure of the receiver domain of the histidine kinase cki12 from arabidopsis thaliana complexed with mg2+ 103 c2hqoA_ not modelled 99.5 8 PDB header:signaling protein Chain: A: PDB Molecule:putative transcriptional regulator; PDBTitle: structure of a atypical orphan response regulator protein revealed a2 new phosphorylation-independent regulatory mechanism 104 c3hdvB_ not modelled 99.5 16 PDB header:transcription regulator Chain: B: PDB Molecule:response regulator; PDBTitle: crystal structure of response regulator receiver protein from2 pseudomonas putida 105 c3cg0A_ not modelled 99.5 7 PDB header:lyase Chain: A: PDB Molecule:response regulator receiver modulated diguanylate cyclase PDBTitle: crystal structure of signal receiver domain of modulated diguanylate2 cyclase from desulfovibrio desulfuricans g20, an example of alternate3 folding 106 c2krfB_ not modelled 99.4 30 PDB header:transcription Chain: B: PDB Molecule:transcriptional regulatory protein coma; PDBTitle: nmr solution structure of the dna binding domain of competence protein2 a 107 c3gl9B_ not modelled 99.4 11 PDB header:signaling protein Chain: B: PDB Molecule:response regulator; PDBTitle: the structure of a histidine kinase-response regulator2 complex sheds light into two-component signaling and3 reveals a novel cis autophosphorylation mechanism 108 d1fsea_ not modelled 99.4 34 Fold:DNA/RNA-binding 3-helical bundle Superfamily:C-terminal effector domain of the bipartite response regulators Family:GerE-like (LuxR/UhpA family of transcriptional regulators) 109 d1k68a_ not modelled 99.4 11 Fold:Flavodoxin-like Superfamily:CheY-like Family:CheY-related 110 d1a04a1 not modelled 99.4 35 Fold:DNA/RNA-binding 3-helical bundle Superfamily:C-terminal effector domain of the bipartite response regulators Family:GerE-like (LuxR/UhpA family of transcriptional regulators) 111 d1l3la1 not modelled 99.4 18 Fold:DNA/RNA-binding 3-helical bundle Superfamily:C-terminal effector domain of the bipartite response regulators Family:GerE-like (LuxR/UhpA family of transcriptional regulators) 112 d1yioa1 not modelled 99.4 27 Fold:DNA/RNA-binding 3-helical bundle Superfamily:C-terminal effector domain of the bipartite response regulators Family:GerE-like (LuxR/UhpA family of transcriptional regulators) 113 c3n0rA_ not modelled 99.4 12 PDB header:signaling protein Chain: A: PDB Molecule:response regulator; PDBTitle: structure of the phyr stress response regulator at 1.25 angstrom2 resolution 114 c3luaA_ not modelled 99.4 11 PDB header:transcription regulator Chain: A: PDB Molecule:response regulator receiver protein; PDBTitle: crystal structure of a signal receiver domain of two component signal2 transduction (histidine kinase) from clostridium thermocellum 115 c3kyiB_ not modelled 99.4 16 PDB header:transferase Chain: B: PDB Molecule:chey6 protein; PDBTitle: crystal structure of the phosphorylated p1 domain of chea3 in complex2 with chey6 from r. sphaeroides 116 c1x3uA_ not modelled 99.4 26 PDB header:transcription Chain: A: PDB Molecule:transcriptional regulatory protein fixj; PDBTitle: solution structure of the c-terminal transcriptional2 activator domain of fixj from sinorhizobium melilot 117 c3eodA_ not modelled 99.4 14 PDB header:signaling protein Chain: A: PDB Molecule:protein hnr; PDBTitle: crystal structure of n-terminal domain of e. coli rssb 118 c3nhmA_ not modelled 99.4 9 PDB header:signaling protein Chain: A: PDB Molecule:response regulator; PDBTitle: crystal structure of a response regulator from myxococcus xanthus 119 c3eqzB_ not modelled 99.4 11 PDB header:structural genomics, unknown function Chain: B: PDB Molecule:response regulator; PDBTitle: crystal structure of a response regulator from colwellia2 psychrerythraea 120 c2rnjA_ not modelled 99.4 38 PDB header:transcription Chain: A: PDB Molecule:response regulator protein vrar; PDBTitle: nmr structure of the s. aureus vrar dna binding domain