Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	E	Q	P	V	I	P	V	R	N	I	Y	Y	M	L	T	Y	A	W	G	Y	L	Q	E	I	K	Q	A	N	L	E	A	I	P	G	N	N	L	L	D	I	L	G	Y	V	L	N	K	G	V	L	Q	L	S	R	R	G	L	E	L
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?													?		?	?		?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		D	Y	N	P	N	T	E	I	I	P	G	I	K	G	R	I	E	F	A	K	T	I	R	G	F	H	L	N	H	G	K	T	V	S	T	F	D	M	L	N	E	D	T	L	A	N	R	I	I	K	S	T	L	A	I	L	I	K	H	E
Secondary structure
SS confidence
Disorder						?		?	?	?																				?																															?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		K	L	N	S	T	I	R	D	E	A	R	S	L	Y	R	K	L	P	G	I	S	T	L	H	L	T	P	Q	H	F	S	Y	L	N	G	G	K	N	T	R	Y	Y	K	F	V	I	S	V	C	K	F	I	V	N	N	S	I	P	G	Q
Secondary structure
SS confidence
Disorder		?	?	?																																																					?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		N	K	G	H	Y	R	F	Y	D	F	E	R	N	E	K	E	M	S	L	L	Y	Q	K	F	L	Y	E	F	C	R	R	E	L	T	S	A	N	T	T	R	S	Y	L	K	W	D	A	S	S	I	S	D	Q	S	L	N	L	L	P	R
Secondary structure
SS confidence
Disorder		?	?	?	?	?																														?	?	?	?	?	?	?							?	?	?		?			?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		M	E	T	D	I	T	I	R	S	S	E	K	I	L	I	V	D	A	K	Y	Y	K	S	I	F	S	R	R	M	G	T	E	K	F	H	S	Q	N	L	Y	Q	L	M	N	Y	L	W	S	L	K	P	E	N	G	E	N	I	G	G	L
Secondary structure
SS confidence
Disorder																										?	?	?	?	?	?	?	?	?	?	?																	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.
Sequence		L	I	Y	P	H	V	D	T	A	V	K	H	R	Y	K	I	N	G	F	D	I	G	L	C	T	V	N	L	G	Q	E	W	P	C	I	H	Q	E	L	L	D	I	F	D	E	Y	L	K
Secondary structure
SS confidence
Disorder											?	?																																				?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 2vld chain A Region: 202 - 308 Aligned: 87 Modelled: 107 Confidence: 88.9% Identity: 20% PDB header:hydrolase Chain: A: PDB Molecule:upf0286 protein pyrab01260; PDBTitle: crystal structure of a repair endonuclease from pyrococcus2 abyssi Phyre2 PDB 1xmx chain A Region: 201 - 302 Aligned: 88 Modelled: 102 Confidence: 70.7% Identity: 13% Fold: Restriction endonuclease-like Superfamily: Restriction endonuclease-like Family: Hypothetical protein VC1899 Phyre2 PDB 2fok chain A domain 4 Region: 198 - 286 Aligned: 64 Modelled: 72 Confidence: 69.0% Identity: 17% Fold: Restriction endonuclease-like Superfamily: Restriction endonuclease-like Family: Restriction endonuclease FokI, C-terminal (catalytic) domain Phyre2 PDB 2wj0 chain B Region: 243 - 292 Aligned: 44 Modelled: 50 Confidence: 64.5% Identity: 18% PDB header:hydrolase/dna Chain: B: PDB Molecule:archaeal hjc; PDBTitle: crystal structures of holliday junction resolvases from2 archaeoglobus fulgidus bound to dna substrate Phyre2 PDB 1fok chain A Region: 198 - 286 Aligned: 64 Modelled: 72 Confidence: 62.2% Identity: 17% PDB header:hydrolase/dna Chain: A: PDB Molecule:protein (foki restriction endonucleas); PDBTitle: structure of restriction endonuclease foki bound to dna Phyre2 PDB 3a9f chain A Region: 256 - 288 Aligned: 33 Modelled: 33 Confidence: 56.6% Identity: 12% PDB header:electron transport Chain: A: PDB Molecule:cytochrome c; PDBTitle: crystal structure of the c-terminal domain of cytochrome cz2 from chlorobium tepidum Phyre2 PDB 1y88 chain A Region: 201 - 302 Aligned: 81 Modelled: 102 Confidence: 52.7% Identity: 9% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:hypothetical protein af1548; PDBTitle: crystal structure of protein of unknown function af1548 Phyre2 PDB 1hh1 chain A Region: 239 - 292 Aligned: 50 Modelled: 54 Confidence: 50.5% Identity: 14% Fold: Restriction endonuclease-like Superfamily: Restriction endonuclease-like Family: Hjc-like Phyre2 PDB 1ob8 chain A Region: 243 - 298 Aligned: 50 Modelled: 56 Confidence: 39.4% Identity: 16% Fold: Restriction endonuclease-like Superfamily: Restriction endonuclease-like Family: Hjc-like Phyre2 PDB 1y88 chain A domain 2 Region: 198 - 302 Aligned: 84 Modelled: 105 Confidence: 36.7% Identity: 6% Fold: Restriction endonuclease-like Superfamily: Restriction endonuclease-like Family: MRR-like Phyre2 PDB 1gef chain A Region: 243 - 291 Aligned: 43 Modelled: 49 Confidence: 33.5% Identity: 14% Fold: Restriction endonuclease-like Superfamily: Restriction endonuclease-like Family: Hjc-like Phyre2 PDB 2fhy chain L Region: 277 - 316 Aligned: 28 Modelled: 40 Confidence: 32.5% Identity: 21% PDB header:hydrolase Chain: L: PDB Molecule:fructose-1,6-bisphosphatase 1; PDBTitle: structure of human liver fpbase complexed with a novel2 benzoxazole as allosteric inhibitor Phyre2 PDB 1spi chain A Region: 277 - 316 Aligned: 28 Modelled: 40 Confidence: 30.1% Identity: 21% Fold: Carbohydrate phosphatase Superfamily: Carbohydrate phosphatase Family: Inositol monophosphatase/fructose-1,6-bisphosphatase-like Phyre2 PDB 1nuw chain A Region: 277 - 316 Aligned: 28 Modelled: 40 Confidence: 28.9% Identity: 25% Fold: Carbohydrate phosphatase Superfamily: Carbohydrate phosphatase Family: Inositol monophosphatase/fructose-1,6-bisphosphatase-like Phyre2 PDB 2gq1 chain A Region: 277 - 316 Aligned: 28 Modelled: 40 Confidence: 28.8% Identity: 18% PDB header:hydrolase Chain: A: PDB Molecule:fructose-1,6-bisphosphatase; PDBTitle: crystal structure of recombinant type i fructose-1,6-bisphosphatase2 from escherichia coli complexed with sulfate ions Phyre2 PDB 1bk4 chain A Region: 277 - 316 Aligned: 28 Modelled: 40 Confidence: 28.6% Identity: 21% Fold: Carbohydrate phosphatase Superfamily: Carbohydrate phosphatase Family: Inositol monophosphatase/fructose-1,6-bisphosphatase-like Phyre2 PDB 1fta chain A Region: 277 - 316 Aligned: 28 Modelled: 40 Confidence: 28.2% Identity: 21% Fold: Carbohydrate phosphatase Superfamily: Carbohydrate phosphatase Family: Inositol monophosphatase/fructose-1,6-bisphosphatase-like Phyre2 PDB 2e52 chain A Region: 198 - 313 Aligned: 92 Modelled: 103 Confidence: 21.5% Identity: 14% PDB header:hydrolase/dna Chain: A: PDB Molecule:type ii restriction enzyme hindiii; PDBTitle: crystal structural analysis of hindiii restriction endonuclease in2 complex with cognate dna at 2.0 angstrom resolution Phyre2 PDB 3h4r chain A Region: 231 - 348 Aligned: 104 Modelled: 118 Confidence: 17.9% Identity: 16% PDB header:hydrolase Chain: A: PDB Molecule:exodeoxyribonuclease 8; PDBTitle: crystal structure of e. coli rece exonuclease Phyre2 PDB 2l2o chain A Region: 163 - 210 Aligned: 48 Modelled: 48 Confidence: 15.4% Identity: 6% PDB header:unknown function Chain: A: PDB Molecule:upf0727 protein c6orf115; PDBTitle: solution structure of human hspc280 protein Phyre2 1 c2vldA_ 2 d1xmxa_ 3 d2foka4 4 c2wj0B_ 5 c1fokA_ 6 c3a9fA_ 7 c1y88A_ 8 d1hh1a_ 9 d1ob8a_ 10 d1y88a2 11 d1gefa_ 12 c2fhyL_ 13 d1spia_ 14 d1nuwa_ 15 c2gq1A_ 16 d1bk4a_ 17 d1ftaa_ 18 c2e52A_ 19 c3h4rA_ 20 c2l2oA_ 21 22 23 24 25 26 27

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 c2vldA_ 88.9 20 PDB header:hydrolase Chain: A: PDB Molecule:upf0286 protein pyrab01260; PDBTitle: crystal structure of a repair endonuclease from pyrococcus2 abyssi 2 d1xmxa_ 70.7 13 Fold:Restriction endonuclease-like Superfamily:Restriction endonuclease-like Family:Hypothetical protein VC1899 3 d2foka4 69.0 17 Fold:Restriction endonuclease-like Superfamily:Restriction endonuclease-like Family:Restriction endonuclease FokI, C-terminal (catalytic) domain 4 c2wj0B_ 64.5 18 PDB header:hydrolase/dna Chain: B: PDB Molecule:archaeal hjc; PDBTitle: crystal structures of holliday junction resolvases from2 archaeoglobus fulgidus bound to dna substrate 5 c1fokA_ 62.2 17 PDB header:hydrolase/dna Chain: A: PDB Molecule:protein (foki restriction endonucleas); PDBTitle: structure of restriction endonuclease foki bound to dna 6 c3a9fA_ 56.6 12 PDB header:electron transport Chain: A: PDB Molecule:cytochrome c; PDBTitle: crystal structure of the c-terminal domain of cytochrome cz2 from chlorobium tepidum 7 c1y88A_ 52.7 9 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:hypothetical protein af1548; PDBTitle: crystal structure of protein of unknown function af1548 8 d1hh1a_ 50.5 14 Fold:Restriction endonuclease-like Superfamily:Restriction endonuclease-like Family:Hjc-like 9 d1ob8a_ 39.4 16 Fold:Restriction endonuclease-like Superfamily:Restriction endonuclease-like Family:Hjc-like 10 d1y88a2 36.7 6 Fold:Restriction endonuclease-like Superfamily:Restriction endonuclease-like Family:MRR-like 11 d1gefa_ 33.5 14 Fold:Restriction endonuclease-like Superfamily:Restriction endonuclease-like Family:Hjc-like 12 c2fhyL_ 32.5 21 PDB header:hydrolase Chain: L: PDB Molecule:fructose-1,6-bisphosphatase 1; PDBTitle: structure of human liver fpbase complexed with a novel2 benzoxazole as allosteric inhibitor 13 d1spia_ 30.1 21 Fold:Carbohydrate phosphatase Superfamily:Carbohydrate phosphatase Family:Inositol monophosphatase/fructose-1,6-bisphosphatase-like 14 d1nuwa_ 28.9 25 Fold:Carbohydrate phosphatase Superfamily:Carbohydrate phosphatase Family:Inositol monophosphatase/fructose-1,6-bisphosphatase-like 15 c2gq1A_ 28.8 18 PDB header:hydrolase Chain: A: PDB Molecule:fructose-1,6-bisphosphatase; PDBTitle: crystal structure of recombinant type i fructose-1,6-bisphosphatase2 from escherichia coli complexed with sulfate ions 16 d1bk4a_ 28.6 21 Fold:Carbohydrate phosphatase Superfamily:Carbohydrate phosphatase Family:Inositol monophosphatase/fructose-1,6-bisphosphatase-like 17 d1ftaa_ 28.2 21 Fold:Carbohydrate phosphatase Superfamily:Carbohydrate phosphatase Family:Inositol monophosphatase/fructose-1,6-bisphosphatase-like 18 c2e52A_ 21.5 14 PDB header:hydrolase/dna Chain: A: PDB Molecule:type ii restriction enzyme hindiii; PDBTitle: crystal structural analysis of hindiii restriction endonuclease in2 complex with cognate dna at 2.0 angstrom resolution 19 c3h4rA_ 17.9 16 PDB header:hydrolase Chain: A: PDB Molecule:exodeoxyribonuclease 8; PDBTitle: crystal structure of e. coli rece exonuclease 20 c2l2oA_ 15.4 6 PDB header:unknown function Chain: A: PDB Molecule:upf0727 protein c6orf115; PDBTitle: solution structure of human hspc280 protein 21 c3h1tA_ not modelled 12.9 22 PDB header:hydrolase Chain: A: PDB Molecule:type i site-specific restriction-modification PDBTitle: the fragment structure of a putative hsdr subunit of a type2 i restriction enzyme from vibrio vulnificus yj016 22 d1c3ha_ not modelled 12.5 30 Fold:TNF-like Superfamily:TNF-like Family:TNF-like 23 d1d9qa_ not modelled 11.9 22 Fold:Carbohydrate phosphatase Superfamily:Carbohydrate phosphatase Family:Inositol monophosphatase/fructose-1,6-bisphosphatase-like 24 c2krhA_ not modelled 10.8 9 PDB header:actin-binding protein Chain: A: PDB Molecule:actin-binding rho-activating protein; PDBTitle: structure of the c-terminal actin binding domain of abra 25 d2bu3a1 not modelled 7.9 12 Fold:Cysteine proteinases Superfamily:Cysteine proteinases Family:Phytochelatin synthase 26 d1w36b3 not modelled 5.9 16 Fold:Restriction endonuclease-like Superfamily:Restriction endonuclease-like Family:Exodeoxyribonuclease V beta chain (RecB), C-terminal domain 27 d1pk6a_ not modelled 5.5 50 Fold:TNF-like Superfamily:TNF-like Family:TNF-like

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

---

Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0