Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	N	R	E	K	G	V	S	S	L	A	L	V	L	M	L	L	V	L	G	S	L	L	L	Q	G	M	S	Q	Q	D	R	S	F	A	S	R	V	S	M	E	S	Q	S	L	R	R	Q	A	I	V	Q	S	A	L	A	W	G	K	M
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?																																																				?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		H	C	W	Q	T	Q	P	A	V	Q	C	S	Q	Y	A	E	T	D	A	Q	V	C	L	R	L	L	A	D	N	E	A	L	L	I	A	G	Y	E	G	V	S	L	W	R	T	G	E	V	I	D	G	N	I	V	F	S	P	R	G	W
Secondary structure
SS confidence
Disorder		?	?		?	?	?	?	?								?	?		?									?	?	?	?	?		?	?															?	?	?				?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.
Sequence		S	D	F	C	P	L	K	E	R	A	L	C	Q	L	P
Secondary structure
SS confidence
Disorder										?	?	?	?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 2vxg chain B Region: 31 - 54 Aligned: 24 Modelled: 24 Confidence: 34.1% Identity: 13% PDB header:gene regulation Chain: B: PDB Molecule:cg6181-pa, isoform a; PDBTitle: crystal structure of the conserved c-terminal region of ge-2 1 Phyre2 PDB 1v06 chain A Region: 118 - 135 Aligned: 18 Modelled: 18 Confidence: 21.3% Identity: 33% PDB header:dna-binding protein Chain: A: PDB Molecule:hmg box-containing protein 1; PDBTitle: axh domain of the transcription factor hbp1 from m.musculus Phyre2 PDB 2px2 chain B Region: 117 - 124 Aligned: 8 Modelled: 8 Confidence: 12.3% Identity: 38% PDB header:transferase Chain: B: PDB Molecule:genome polyprotein [contains: capsid protein c PDBTitle: crystal structure of the murray valley encephalitis virus2 ns5 2'-o methyltransferase domain in complex with sah3 (monoclinic form 1) Phyre2 PDB 3gcz chain A Region: 117 - 124 Aligned: 8 Modelled: 8 Confidence: 11.3% Identity: 38% PDB header:transferase Chain: A: PDB Molecule:polyprotein; PDBTitle: yokose virus methyltransferase in complex with adomet Phyre2 PDB 2yu1 chain A Region: 103 - 120 Aligned: 18 Modelled: 18 Confidence: 10.3% Identity: 28% PDB header:oxidoreductase Chain: A: PDB Molecule:jmjc domain-containing histone demethylation protein 1a; PDBTitle: crystal structure of hjhdm1a complexed with a-ketoglutarate Phyre2 PDB 2ds2 chain A Region: 71 - 86 Aligned: 16 Modelled: 16 Confidence: 10.3% Identity: 19% PDB header:plant protein Chain: A: PDB Molecule:sweet protein mabinlin-2 chain a; PDBTitle: crystal structure of mabinlin ii Phyre2 PDB 2kix chain D Region: 16 - 29 Aligned: 14 Modelled: 14 Confidence: 10.2% Identity: 21% PDB header:transport protein Chain: D: PDB Molecule:bm2 protein; PDBTitle: channel domain of bm2 protein from influenza b virus Phyre2 PDB 3n9m chain C Region: 103 - 120 Aligned: 18 Modelled: 18 Confidence: 10.2% Identity: 22% PDB header:oxidoreductase Chain: C: PDB Molecule:putative uncharacterized protein; PDBTitle: cekdm7a from c.elegans, alone Phyre2 PDB 1yel chain A domain 1 Region: 105 - 128 Aligned: 23 Modelled: 24 Confidence: 10.0% Identity: 13% Fold: DNA-binding pseudobarrel domain Superfamily: DNA-binding pseudobarrel domain Family: B3 DNA binding domain Phyre2 PDB 3eva chain A Region: 117 - 124 Aligned: 8 Modelled: 8 Confidence: 9.6% Identity: 25% PDB header:transferase Chain: A: PDB Molecule:rna-directed rna polymerase ns5; PDBTitle: crystal structure of yellow fever virus methyltransferase complexed2 with s-adenosyl-l-homocysteine Phyre2 PDB 1i94 chain L Region: 75 - 129 Aligned: 55 Modelled: 55 Confidence: 9.4% Identity: 16% Fold: OB-fold Superfamily: Nucleic acid-binding proteins Family: Cold shock DNA-binding domain-like Phyre2 PDB 1waz chain A Region: 119 - 123 Aligned: 5 Modelled: 5 Confidence: 8.8% Identity: 40% PDB header:transport protein Chain: A: PDB Molecule:merf; PDBTitle: nmr structure determination of the bacterial mercury2 transporter, merf, in micelles Phyre2 PDB 3lkz chain B Region: 115 - 124 Aligned: 10 Modelled: 10 Confidence: 8.8% Identity: 20% PDB header:viral protein Chain: B: PDB Molecule:non-structural protein 5; PDBTitle: structural and functional analyses of a conserved hydrophobic pocket2 of flavivirus methyltransferase Phyre2 PDB 2p41 chain A domain 1 Region: 115 - 124 Aligned: 10 Modelled: 10 Confidence: 8.5% Identity: 40% Fold: S-adenosyl-L-methionine-dependent methyltransferases Superfamily: S-adenosyl-L-methionine-dependent methyltransferases Family: mRNA cap methylase Phyre2 PDB 2jo1 chain A Region: 7 - 26 Aligned: 20 Modelled: 20 Confidence: 7.5% Identity: 25% PDB header:hydrolase regulator Chain: A: PDB Molecule:phospholemman; PDBTitle: structure of the na,k-atpase regulatory protein fxyd1 in2 micelles Phyre2 PDB 2jp3 chain A Region: 8 - 24 Aligned: 17 Modelled: 17 Confidence: 7.3% Identity: 24% PDB header:transcription Chain: A: PDB Molecule:fxyd domain-containing ion transport regulator 4; PDBTitle: solution structure of the human fxyd4 (chif) protein in sds2 micelles Phyre2 PDB 1s3g chain A domain 2 Region: 121 - 134 Aligned: 14 Modelled: 14 Confidence: 7.0% Identity: 21% Fold: Rubredoxin-like Superfamily: Microbial and mitochondrial ADK, insert "zinc finger" domain Family: Microbial and mitochondrial ADK, insert "zinc finger" domain Phyre2 PDB 1zin chain A domain 2 Region: 121 - 134 Aligned: 14 Modelled: 14 Confidence: 6.7% Identity: 21% Fold: Rubredoxin-like Superfamily: Microbial and mitochondrial ADK, insert "zinc finger" domain Family: Microbial and mitochondrial ADK, insert "zinc finger" domain Phyre2 PDB 2h3o chain A Region: 119 - 123 Aligned: 5 Modelled: 5 Confidence: 6.6% Identity: 40% PDB header:membrane protein Chain: A: PDB Molecule:merf; PDBTitle: structure of merft, a membrane protein with two trans-2 membrane helices Phyre2 PDB 1p3j chain A domain 2 Region: 121 - 134 Aligned: 14 Modelled: 14 Confidence: 6.5% Identity: 29% Fold: Rubredoxin-like Superfamily: Microbial and mitochondrial ADK, insert "zinc finger" domain Family: Microbial and mitochondrial ADK, insert "zinc finger" domain Phyre2 1 c2vxgB_ 2 c1v06A_ 3 c2px2B_ 4 c3gczA_ 5 c2yu1A_ 6 c2ds2A_ 7 c2kixD_ 8 c3n9mC_ 9 d1yela1 10 c3evaA_ 11 d1i94l_ 12 c1wazA_ 13 c3lkzB_ 14 d2p41a1 15 c2jo1A_ 16 c2jp3A_ 17 d1s3ga2 18 d1zina2 19 c2h3oA_ 20 d1p3ja2 21 22 23 24 25 26 27 28 29 30 31

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 c2vxgB_ 34.1 13 PDB header:gene regulation Chain: B: PDB Molecule:cg6181-pa, isoform a; PDBTitle: crystal structure of the conserved c-terminal region of ge-2 1 2 c1v06A_ 21.3 33 PDB header:dna-binding protein Chain: A: PDB Molecule:hmg box-containing protein 1; PDBTitle: axh domain of the transcription factor hbp1 from m.musculus 3 c2px2B_ 12.3 38 PDB header:transferase Chain: B: PDB Molecule:genome polyprotein [contains: capsid protein c PDBTitle: crystal structure of the murray valley encephalitis virus2 ns5 2'-o methyltransferase domain in complex with sah3 (monoclinic form 1) 4 c3gczA_ 11.3 38 PDB header:transferase Chain: A: PDB Molecule:polyprotein; PDBTitle: yokose virus methyltransferase in complex with adomet 5 c2yu1A_ 10.3 28 PDB header:oxidoreductase Chain: A: PDB Molecule:jmjc domain-containing histone demethylation protein 1a; PDBTitle: crystal structure of hjhdm1a complexed with a-ketoglutarate 6 c2ds2A_ 10.3 19 PDB header:plant protein Chain: A: PDB Molecule:sweet protein mabinlin-2 chain a; PDBTitle: crystal structure of mabinlin ii 7 c2kixD_ 10.2 21 PDB header:transport protein Chain: D: PDB Molecule:bm2 protein; PDBTitle: channel domain of bm2 protein from influenza b virus 8 c3n9mC_ 10.2 22 PDB header:oxidoreductase Chain: C: PDB Molecule:putative uncharacterized protein; PDBTitle: cekdm7a from c.elegans, alone 9 d1yela1 10.0 13 Fold:DNA-binding pseudobarrel domain Superfamily:DNA-binding pseudobarrel domain Family:B3 DNA binding domain 10 c3evaA_ 9.6 25 PDB header:transferase Chain: A: PDB Molecule:rna-directed rna polymerase ns5; PDBTitle: crystal structure of yellow fever virus methyltransferase complexed2 with s-adenosyl-l-homocysteine 11 d1i94l_ 9.4 16 Fold:OB-fold Superfamily:Nucleic acid-binding proteins Family:Cold shock DNA-binding domain-like 12 c1wazA_ 8.8 40 PDB header:transport protein Chain: A: PDB Molecule:merf; PDBTitle: nmr structure determination of the bacterial mercury2 transporter, merf, in micelles 13 c3lkzB_ 8.8 20 PDB header:viral protein Chain: B: PDB Molecule:non-structural protein 5; PDBTitle: structural and functional analyses of a conserved hydrophobic pocket2 of flavivirus methyltransferase 14 d2p41a1 8.5 40 Fold:S-adenosyl-L-methionine-dependent methyltransferases Superfamily:S-adenosyl-L-methionine-dependent methyltransferases Family:mRNA cap methylase 15 c2jo1A_ 7.5 25 PDB header:hydrolase regulator Chain: A: PDB Molecule:phospholemman; PDBTitle: structure of the na,k-atpase regulatory protein fxyd1 in2 micelles 16 c2jp3A_ 7.3 24 PDB header:transcription Chain: A: PDB Molecule:fxyd domain-containing ion transport regulator 4; PDBTitle: solution structure of the human fxyd4 (chif) protein in sds2 micelles 17 d1s3ga2 7.0 21 Fold:Rubredoxin-like Superfamily:Microbial and mitochondrial ADK, insert "zinc finger" domain Family:Microbial and mitochondrial ADK, insert "zinc finger" domain 18 d1zina2 6.7 21 Fold:Rubredoxin-like Superfamily:Microbial and mitochondrial ADK, insert "zinc finger" domain Family:Microbial and mitochondrial ADK, insert "zinc finger" domain 19 c2h3oA_ 6.6 40 PDB header:membrane protein Chain: A: PDB Molecule:merf; PDBTitle: structure of merft, a membrane protein with two trans-2 membrane helices 20 d1p3ja2 6.5 29 Fold:Rubredoxin-like Superfamily:Microbial and mitochondrial ADK, insert "zinc finger" domain Family:Microbial and mitochondrial ADK, insert "zinc finger" domain 21 d2uubl1 not modelled 6.1 16 Fold:OB-fold Superfamily:Nucleic acid-binding proteins Family:Cold shock DNA-binding domain-like 22 d1t1ra3 not modelled 6.1 75 Fold:NAD(P)-binding Rossmann-fold domains Superfamily:NAD(P)-binding Rossmann-fold domains Family:Glyceraldehyde-3-phosphate dehydrogenase-like, N-terminal domain 23 c3pu3A_ not modelled 5.7 33 PDB header:protein binding Chain: A: PDB Molecule:phd finger protein 2; PDBTitle: phf2 jumonji domain-nog complex 24 d1xvha1 not modelled 5.7 22 Fold:immunoglobulin/albumin-binding domain-like Superfamily:Bacterial immunoglobulin/albumin-binding domains Family:GA module, an albumin-binding domain 25 d2qall1 not modelled 5.6 18 Fold:OB-fold Superfamily:Nucleic acid-binding proteins Family:Cold shock DNA-binding domain-like 26 c3pesA_ not modelled 5.5 25 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:uncharacterized protein gp49; PDBTitle: crystal structure of uncharacterized protein from pseudomonas phage2 yua 27 c1ujlA_ not modelled 5.5 50 PDB header:membrane protein Chain: A: PDB Molecule:potassium voltage-gated channel subfamily h PDBTitle: solution structure of the herg k+ channel s5-p2 extracellular linker 28 c2p3qA_ not modelled 5.3 50 PDB header:viral protein,transferase Chain: A: PDB Molecule:type ii methyltransferase; PDBTitle: crystal structure of dengue methyltransferase in complex with gpppg2 and s-adenosyl-l-homocysteine 29 d1akya2 not modelled 5.2 29 Fold:Rubredoxin-like Superfamily:Microbial and mitochondrial ADK, insert "zinc finger" domain Family:Microbial and mitochondrial ADK, insert "zinc finger" domain 30 c3kv5D_ not modelled 5.2 33 PDB header:h3k4me3 binding protein, transferase Chain: D: PDB Molecule:jmjc domain-containing histone demethylation PDBTitle: structure of kiaa1718, human jumonji demethylase, in complex2 with n-oxalylglycine 31 c3alrA_ not modelled 5.1 33 PDB header:metal binding protein Chain: A: PDB Molecule:nanos protein; PDBTitle: crystal structure of nanos

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

Transmembrane helix prediction

Transmembrane helices have been predicted in your sequence to adopt the topology shown below

---

Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0