Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	G	I	F	S	I	A	N	Q	H	I	R	F	A	V	K	L	A	T	A	I	V	L	A	L	F	V	G	F	H	F	Q	L	E	T	P	R	W	A	V	L	T	A	A	I	V	A	A	G	T	A	F	A	A	G	G	E	P	Y	S
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?																																										?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		G	A	I	R	Y	R	G	F	L	R	I	I	G	T	F	I	G	C	I	A	G	L	V	I	I	I	A	M	I	R	A	P	L	L	M	I	L	V	C	C	I	W	A	G	F	C	T	W	I	S	S	L	V	R	I	E	N	S	Y	A
Secondary structure
SS confidence
Disorder		?																																																			?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		W	G	L	A	G	Y	T	A	L	I	I	V	I	T	I	Q	P	E	P	L	L	T	P	Q	F	A	V	E	R	C	S	E	I	V	I	G	I	V	C	A	I	M	A	D	L	L	F	S	P	R	S	I	K	Q	E	V	D	R	E	L
Secondary structure
SS confidence
Disorder															?	?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		E	S	L	L	V	A	Q	Y	Q	L	M	Q	L	C	I	K	H	G	D	G	E	V	V	D	K	A	W	G	D	L	V	R	R	T	T	A	L	Q	G	M	R	S	N	L	N	M	E	S	S	R	W	A	R	A	N	R	R	L	K	A
Secondary structure
SS confidence
Disorder																				?	?	?	?	?																					?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		I	N	T	L	S	L	T	L	I	T	Q	S	C	E	T	Y	L	I	Q	N	T	R	P	E	L	I	T	D	T	F	R	E	F	F	D	T	P	V	E	T	A	Q	D	V	H	K	Q	L	K	R	L	R	R	V	I	A	W	T	G	E
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		R	E	T	P	V	T	I	Y	S	W	V	A	A	A	T	R	Y	Q	L	L	K	R	G	V	I	S	N	T	K	I	N	A	T	E	E	E	I	L	Q	G	E	P	E	V	K	V	E	S	A	E	R	H	H	A	M	V	N	F	W	R
Secondary structure
SS confidence
Disorder																																	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420
Sequence		T	T	L	S	C	I	L	G	T	L	F	W	L	W	T	G	W	T	S	G	S	G	A	M	V	M	I	A	V	V	T	S	L	A	M	R	L	P	N	P	R	M	V	A	I	D	F	I	Y	G	T	L	A	A	L	P	L	G	L	L
Secondary structure
SS confidence
Disorder																																			?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.	.	.	.	.	.	.	460	.	.	.	.	.	.	.	.	.	470	.	.	.	.	.	.	.	.	.	480
Sequence		Y	F	L	V	I	I	P	N	T	Q	Q	S	M	L	L	L	C	I	S	L	A	V	L	G	F	F	L	G	I	E	V	Q	K	R	R	L	G	S	M	G	A	L	A	S	T	I	N	I	I	V	L	D	N	P	M	T	F	H	F	S
Secondary structure
SS confidence
Disorder																															?	?	?		?															?		?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	490	.	.	.	.	.	.	.	.	.	500	.	.	.	.	.	.	.	.	.	510	.	.	.	.	.	.	.	.	.	520	.	.	.	.	.	.	.	.	.	530	.	.	.	.	.	.	.	.	.	540
Sequence		Q	F	L	D	S	A	L	G	Q	I	V	G	C	V	L	A	F	T	V	I	L	L	V	R	D	K	S	R	D	R	T	G	R	V	L	L	N	Q	F	V	S	A	A	V	S	A	M	T	T	N	V	A	R	R	K	E	N	H	L	P
Secondary structure
SS confidence
Disorder																																															?	?		?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	550	.	.	.	.	.	.	.	.	.	560	.	.	.	.	.	.	.	.	.	570	.	.	.	.	.	.	.	.	.	580	.	.	.	.	.	.	.	.	.	590	.	.	.	.	.	.	.	.	.	600
Sequence		A	L	Y	Q	Q	L	F	L	L	M	N	K	F	P	G	D	L	P	K	F	R	L	A	L	T	M	I	I	A	H	Q	R	L	R	D	A	P	I	P	V	N	E	D	L	S	A	F	H	R	Q	M	R	R	T	A	D	H	V	I	S
Secondary structure
SS confidence
Disorder											?	?	?	?	?	?	?	?																						?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	610	.	.	.	.	.	.	.	.	.	620	.	.	.	.	.	.	.	.	.	630	.	.	.	.	.	.	.	.	.	640	.	.	.	.	.	.	.	.	.	650	.	.	.	.	.
Sequence		A	R	S	D	D	K	R	R	R	Y	F	G	Q	L	L	E	E	L	E	I	Y	Q	E	K	L	R	I	W	Q	A	P	P	Q	V	T	E	P	V	N	R	L	A	G	M	L	H	K	Y	Q	H	A	L	T	D	S
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?													?	?	?	?	?	?	?	?	?	?	?	?	?	?	?													?	?	?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 3k07 chain A Region: 8 - 169 Aligned: 137 Modelled: 137 Confidence: 48.0% Identity: 11% PDB header:transport protein Chain: A: PDB Molecule:cation efflux system protein cusa; PDBTitle: crystal structure of cusa Phyre2 PDB 1oy8 chain A Region: 13 - 169 Aligned: 132 Modelled: 132 Confidence: 38.3% Identity: 13% PDB header:membrane protein Chain: A: PDB Molecule:acriflavine resistance protein b; PDBTitle: structural basis of multiple drug binding capacity of the acrb2 multidrug efflux pump Phyre2 PDB 1j4n chain A Region: 351 - 515 Aligned: 165 Modelled: 165 Confidence: 32.8% Identity: 17% Fold: Aquaporin-like Superfamily: Aquaporin-like Family: Aquaporin-like Phyre2 PDB 2r6g chain F domain 1 Region: 408 - 472 Aligned: 65 Modelled: 65 Confidence: 14.2% Identity: 14% Fold: MalF N-terminal region-like Superfamily: MalF N-terminal region-like Family: MalF N-terminal region-like Phyre2 PDB 2ht2 chain B Region: 17 - 182 Aligned: 119 Modelled: 138 Confidence: 14.0% Identity: 15% PDB header:membrane protein Chain: B: PDB Molecule:h(+)/cl(-) exchange transporter clca; PDBTitle: structure of the escherichia coli clc chloride channel2 y445h mutant and fab complex Phyre2 PDB 3k3g chain A Region: 81 - 169 Aligned: 89 Modelled: 89 Confidence: 12.9% Identity: 12% PDB header:transport protein Chain: A: PDB Molecule:urea transporter; PDBTitle: crystal structure of the urea transporter from desulfovibrio vulgaris2 bound to 1,3-dimethylurea Phyre2 PDB 1kpl chain A Region: 17 - 186 Aligned: 123 Modelled: 142 Confidence: 11.8% Identity: 14% Fold: Clc chloride channel Superfamily: Clc chloride channel Family: Clc chloride channel Phyre2 PDB 1rc2 chain A Region: 358 - 508 Aligned: 148 Modelled: 148 Confidence: 10.1% Identity: 14% Fold: Aquaporin-like Superfamily: Aquaporin-like Family: Aquaporin-like Phyre2 PDB 2ksf chain A Region: 411 - 502 Aligned: 91 Modelled: 92 Confidence: 8.6% Identity: 11% PDB header:transferase Chain: A: PDB Molecule:sensor protein kdpd; PDBTitle: backbone structure of the membrane domain of e. coli2 histidine kinase receptor kdpd, center for structures of3 membrane proteins (csmp) target 4312c Phyre2 PDB 3aqp chain B Region: 5 - 169 Aligned: 136 Modelled: 139 Confidence: 7.5% Identity: 18% PDB header:membrane protein Chain: B: PDB Molecule:probable secdf protein-export membrane protein; PDBTitle: crystal structure of secdf, a translocon-associated membrane protein,2 from thermus thrmophilus Phyre2 PDB 3llq chain B Region: 356 - 503 Aligned: 145 Modelled: 148 Confidence: 7.4% Identity: 19% PDB header:membrane protein Chain: B: PDB Molecule:aquaporin z 2; PDBTitle: aquaporin structure from plant pathogen agrobacterium tumerfaciens Phyre2 PDB 1ots chain A Region: 11 - 186 Aligned: 129 Modelled: 148 Confidence: 7.2% Identity: 15% Fold: Clc chloride channel Superfamily: Clc chloride channel Family: Clc chloride channel Phyre2 PDB 3gd8 chain A Region: 358 - 427 Aligned: 67 Modelled: 70 Confidence: 6.5% Identity: 10% PDB header:membrane protein Chain: A: PDB Molecule:aquaporin-4; PDBTitle: crystal structure of human aquaporin 4 at 1.8 and its mechanism of2 conductance Phyre2 PDB 3nd0 chain A Region: 17 - 180 Aligned: 117 Modelled: 136 Confidence: 6.5% Identity: 11% PDB header:transport protein Chain: A: PDB Molecule:sll0855 protein; PDBTitle: x-ray crystal structure of a slow cyanobacterial cl-/h+ antiporter Phyre2 PDB 2e74 chain D domain 2 Region: 401 - 424 Aligned: 24 Modelled: 24 Confidence: 6.2% Identity: 38% Fold: Single transmembrane helix Superfamily: ISP transmembrane anchor Family: ISP transmembrane anchor Phyre2 PDB 1iwg chain A domain 8 Region: 15 - 169 Aligned: 130 Modelled: 130 Confidence: 6.2% Identity: 12% Fold: Multidrug efflux transporter AcrB transmembrane domain Superfamily: Multidrug efflux transporter AcrB transmembrane domain Family: Multidrug efflux transporter AcrB transmembrane domain Phyre2 PDB 1s7b chain A Region: 93 - 173 Aligned: 78 Modelled: 81 Confidence: 5.6% Identity: 10% Fold: Multidrug resistance efflux transporter EmrE Superfamily: Multidrug resistance efflux transporter EmrE Family: Multidrug resistance efflux transporter EmrE Phyre2 PDB 2jp3 chain A Region: 472 - 505 Aligned: 32 Modelled: 34 Confidence: 5.5% Identity: 25% PDB header:transcription Chain: A: PDB Molecule:fxyd domain-containing ion transport regulator 4; PDBTitle: solution structure of the human fxyd4 (chif) protein in sds2 micelles Phyre2 PDB 2jo1 chain A Region: 472 - 503 Aligned: 30 Modelled: 32 Confidence: 5.5% Identity: 27% PDB header:hydrolase regulator Chain: A: PDB Molecule:phospholemman; PDBTitle: structure of the na,k-atpase regulatory protein fxyd1 in2 micelles Phyre2 1 c3k07A_ 2 c1oy8A_ 3 d1j4na_ 4 d2r6gf1 5 c2ht2B_ 6 c3k3gA_ 7 d1kpla_ 8 d1rc2a_ 9 c2ksfA_ 10 c3aqpB_ 11 c3llqB_ 12 d1otsa_ 13 c3gd8A_ 14 c3nd0A_ 15 d2e74d2 16 d1iwga8 17 d1s7ba_ 18 c2jp3A_ 19 c2jo1A_

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 c3k07A_ 48.0 11 PDB header:transport protein Chain: A: PDB Molecule:cation efflux system protein cusa; PDBTitle: crystal structure of cusa 2 c1oy8A_ 38.3 13 PDB header:membrane protein Chain: A: PDB Molecule:acriflavine resistance protein b; PDBTitle: structural basis of multiple drug binding capacity of the acrb2 multidrug efflux pump 3 d1j4na_ 32.8 17 Fold:Aquaporin-like Superfamily:Aquaporin-like Family:Aquaporin-like 4 d2r6gf1 14.2 14 Fold:MalF N-terminal region-like Superfamily:MalF N-terminal region-like Family:MalF N-terminal region-like 5 c2ht2B_ 14.0 15 PDB header:membrane protein Chain: B: PDB Molecule:h(+)/cl(-) exchange transporter clca; PDBTitle: structure of the escherichia coli clc chloride channel2 y445h mutant and fab complex 6 c3k3gA_ 12.9 12 PDB header:transport protein Chain: A: PDB Molecule:urea transporter; PDBTitle: crystal structure of the urea transporter from desulfovibrio vulgaris2 bound to 1,3-dimethylurea 7 d1kpla_ 11.8 14 Fold:Clc chloride channel Superfamily:Clc chloride channel Family:Clc chloride channel 8 d1rc2a_ 10.1 14 Fold:Aquaporin-like Superfamily:Aquaporin-like Family:Aquaporin-like 9 c2ksfA_ 8.6 11 PDB header:transferase Chain: A: PDB Molecule:sensor protein kdpd; PDBTitle: backbone structure of the membrane domain of e. coli2 histidine kinase receptor kdpd, center for structures of3 membrane proteins (csmp) target 4312c 10 c3aqpB_ 7.5 18 PDB header:membrane protein Chain: B: PDB Molecule:probable secdf protein-export membrane protein; PDBTitle: crystal structure of secdf, a translocon-associated membrane protein,2 from thermus thrmophilus 11 c3llqB_ 7.4 19 PDB header:membrane protein Chain: B: PDB Molecule:aquaporin z 2; PDBTitle: aquaporin structure from plant pathogen agrobacterium tumerfaciens 12 d1otsa_ 7.2 15 Fold:Clc chloride channel Superfamily:Clc chloride channel Family:Clc chloride channel 13 c3gd8A_ 6.5 10 PDB header:membrane protein Chain: A: PDB Molecule:aquaporin-4; PDBTitle: crystal structure of human aquaporin 4 at 1.8 and its mechanism of2 conductance 14 c3nd0A_ 6.5 11 PDB header:transport protein Chain: A: PDB Molecule:sll0855 protein; PDBTitle: x-ray crystal structure of a slow cyanobacterial cl-/h+ antiporter 15 d2e74d2 6.2 38 Fold:Single transmembrane helix Superfamily:ISP transmembrane anchor Family:ISP transmembrane anchor 16 d1iwga8 6.2 12 Fold:Multidrug efflux transporter AcrB transmembrane domain Superfamily:Multidrug efflux transporter AcrB transmembrane domain Family:Multidrug efflux transporter AcrB transmembrane domain 17 d1s7ba_ 5.6 10 Fold:Multidrug resistance efflux transporter EmrE Superfamily:Multidrug resistance efflux transporter EmrE Family:Multidrug resistance efflux transporter EmrE 18 c2jp3A_ 5.5 25 PDB header:transcription Chain: A: PDB Molecule:fxyd domain-containing ion transport regulator 4; PDBTitle: solution structure of the human fxyd4 (chif) protein in sds2 micelles 19 c2jo1A_ 5.5 27 PDB header:hydrolase regulator Chain: A: PDB Molecule:phospholemman; PDBTitle: structure of the na,k-atpase regulatory protein fxyd1 in2 micelles

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

Transmembrane helix prediction

Transmembrane helices have been predicted in your sequence to adopt the topology shown below

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0