Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	T	D	M	N	I	L	D	L	F	L	K	A	S	L	L	V	K	L	I	M	L	I	L	I	G	F	S	I	A	S	W	A	I	I	I	Q	R	T	R	I	L	N	A	A	A	R	E	A	E	A	F	E	D	K	F	W	S	G	I
Secondary structure
SS confidence
Disorder		?	?	?	?	?																																								?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		E	L	S	R	L	Y	Q	E	S	Q	G	K	R	D	N	L	T	G	S	E	Q	I	F	Y	S	G	F	K	E	F	V	R	L	H	R	A	N	S	H	A	P	E	A	V	V	E	G	A	S	R	A	M	R	I	S	M	N	R	E	L
Secondary structure
SS confidence
Disorder			?	?								?	?	?	?	?	?																	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		E	N	L	E	T	H	I	P	F	L	G	T	V	G	S	I	S	P	Y	I	G	L	F	G	T	V	W	G	I	M	H	A	F	I	A	L	G	A	V	K	Q	A	T	L	Q	M	V	A	P	G	I	A	E	A	L	I	A	T	A	I
Secondary structure
SS confidence
Disorder						?																																?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230
Sequence		G	L	F	A	A	I	P	A	V	M	A	Y	N	R	L	N	Q	R	V	N	K	L	E	L	N	Y	D	N	F	M	E	E	F	T	A	I	L	H	R	Q	A	F	T	V	S	E	S	N	K	G
Secondary structure
SS confidence
Disorder																																									?	?	?	?	?	?	?	?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 2bbj chain B Region: 35 - 149 Aligned: 107 Modelled: 115 Confidence: 79.2% Identity: 15% PDB header:metal transport/membrane protein Chain: B: PDB Molecule:divalent cation transport-related protein; PDBTitle: crystal structure of the cora mg2+ transporter Phyre2 PDB 1cii chain A Region: 113 - 205 Aligned: 93 Modelled: 93 Confidence: 66.3% Identity: 13% PDB header:transmembrane protein Chain: A: PDB Molecule:colicin ia; PDBTitle: colicin ia Phyre2 PDB 1o5h chain A Region: 159 - 219 Aligned: 58 Modelled: 61 Confidence: 26.4% Identity: 9% Fold: Methenyltetrahydrofolate cyclohydrolase-like Superfamily: Methenyltetrahydrofolate cyclohydrolase-like Family: Methenyltetrahydrofolate cyclohydrolase-like Phyre2 PDB 2ap7 chain A Region: 136 - 148 Aligned: 13 Modelled: 13 Confidence: 25.8% Identity: 46% PDB header:antibiotic Chain: A: PDB Molecule:bombinin h2; PDBTitle: solution structure of bombinin h2 in dpc micelles Phyre2 PDB 2ap8 chain A Region: 136 - 148 Aligned: 13 Modelled: 13 Confidence: 23.8% Identity: 46% PDB header:antibiotic Chain: A: PDB Molecule:bombinin h4; PDBTitle: solution structure of bombinin h4 in dpc micelles Phyre2 PDB 1jt6 chain A domain 1 Region: 169 - 223 Aligned: 51 Modelled: 55 Confidence: 20.9% Identity: 6% Fold: DNA/RNA-binding 3-helical bundle Superfamily: Homeodomain-like Family: Tetracyclin repressor-like, N-terminal domain Phyre2 PDB 1rkt chain A domain 1 Region: 169 - 224 Aligned: 52 Modelled: 56 Confidence: 16.0% Identity: 10% Fold: DNA/RNA-binding 3-helical bundle Superfamily: Homeodomain-like Family: Tetracyclin repressor-like, N-terminal domain Phyre2 PDB 1tt9 chain B Region: 160 - 219 Aligned: 57 Modelled: 57 Confidence: 15.3% Identity: 9% PDB header:transferase, lyase Chain: B: PDB Molecule:formimidoyltransferase-cyclodeaminase PDBTitle: structure of the bifunctional and golgi associated2 formiminotransferase cyclodeaminase octamer Phyre2 PDB 1vi0 chain A domain 1 Region: 169 - 225 Aligned: 53 Modelled: 57 Confidence: 13.7% Identity: 11% Fold: DNA/RNA-binding 3-helical bundle Superfamily: Homeodomain-like Family: Tetracyclin repressor-like, N-terminal domain Phyre2 PDB 1q8d chain A Region: 139 - 147 Aligned: 9 Modelled: 8 Confidence: 8.8% Identity: 67% Fold: GDNF receptor-like Superfamily: GDNF receptor-like Family: GDNF receptor-like Phyre2 PDB 2dg8 chain D Region: 169 - 226 Aligned: 54 Modelled: 58 Confidence: 8.1% Identity: 11% PDB header:gene regulation Chain: D: PDB Molecule:putative tetr-family transcriptional regulatory protein; PDBTitle: crystal structure of the putative trasncriptional regulator sco75182 from streptomyces coelicolor a3(2) Phyre2 PDB 1zk8 chain A domain 1 Region: 169 - 222 Aligned: 50 Modelled: 54 Confidence: 8.1% Identity: 16% Fold: DNA/RNA-binding 3-helical bundle Superfamily: Homeodomain-like Family: Tetracyclin repressor-like, N-terminal domain Phyre2 PDB 1pb6 chain A domain 1 Region: 169 - 219 Aligned: 47 Modelled: 51 Confidence: 7.5% Identity: 13% Fold: DNA/RNA-binding 3-helical bundle Superfamily: Homeodomain-like Family: Tetracyclin repressor-like, N-terminal domain Phyre2 PDB 3s5r chain A Region: 169 - 227 Aligned: 55 Modelled: 59 Confidence: 7.5% Identity: 15% PDB header:dna binding protein Chain: A: PDB Molecule:transcriptional regulator tetr family; PDBTitle: crystal structure of a putative transcriptional regulator of the tetr2 family (syn_02108) from syntrophus aciditrophicus at 2.60 a3 resolution Phyre2 PDB 2fbq chain A domain 1 Region: 169 - 227 Aligned: 55 Modelled: 59 Confidence: 7.0% Identity: 16% Fold: DNA/RNA-binding 3-helical bundle Superfamily: Homeodomain-like Family: Tetracyclin repressor-like, N-terminal domain Phyre2 PDB 3gzi chain A Region: 170 - 228 Aligned: 55 Modelled: 59 Confidence: 6.9% Identity: 11% PDB header:transcription Chain: A: PDB Molecule:transcriptional regulator, tetr family; PDBTitle: crystal structure of a transcriptional regulator of the tetr family2 (shew_3567) from shewanella loihica pv-4 at 2.05 a resolution Phyre2 PDB 2l4g chain A Region: 141 - 151 Aligned: 11 Modelled: 11 Confidence: 6.7% Identity: 45% PDB header:viral protein Chain: A: PDB Molecule:haemagglutinin; PDBTitle: influenza haemagglutinin fusion peptide mutant g13a Phyre2 PDB 1u9n chain A Region: 169 - 228 Aligned: 56 Modelled: 60 Confidence: 6.4% Identity: 16% PDB header:dna binding protein Chain: A: PDB Molecule:transcriptional repressor ethr; PDBTitle: crystal structure of the transcriptional regulator ethr in2 a ligand bound conformation opens therapeutic perspectives3 against tuberculosis and leprosy Phyre2 PDB 2v5e chain A Region: 136 - 147 Aligned: 12 Modelled: 12 Confidence: 6.2% Identity: 50% PDB header:receptor/glycoprotein complex Chain: A: PDB Molecule:gdnf family receptor alpha-1; PDBTitle: the structure of the gdnf\:coreceptor complex\: insights2 into ret signalling and heparin binding. Phyre2 PDB 3fub chain A Region: 136 - 147 Aligned: 12 Modelled: 12 Confidence: 6.2% Identity: 50% PDB header:hormone Chain: A: PDB Molecule:gdnf family receptor alpha-1; PDBTitle: crystal structure of gdnf-gfralpha1 complex Phyre2 1 c2bbjB_ 2 c1ciiA_ 3 d1o5ha_ 4 c2ap7A_ 5 c2ap8A_ 6 d1jt6a1 7 d1rkta1 8 c1tt9B_ 9 d1vi0a1 10 d1q8da_ 11 c2dg8D_ 12 d1zk8a1 13 d1pb6a1 14 c3s5rA_ 15 d2fbqa1 16 c3gziA_ 17 c2l4gA_ 18 c1u9nA_ 19 c2v5eA_ 20 c3fubA_ 21 22 23 24 25

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 c2bbjB_ 79.2 15 PDB header:metal transport/membrane protein Chain: B: PDB Molecule:divalent cation transport-related protein; PDBTitle: crystal structure of the cora mg2+ transporter 2 c1ciiA_ 66.3 13 PDB header:transmembrane protein Chain: A: PDB Molecule:colicin ia; PDBTitle: colicin ia 3 d1o5ha_ 26.4 9 Fold:Methenyltetrahydrofolate cyclohydrolase-like Superfamily:Methenyltetrahydrofolate cyclohydrolase-like Family:Methenyltetrahydrofolate cyclohydrolase-like 4 c2ap7A_ 25.8 46 PDB header:antibiotic Chain: A: PDB Molecule:bombinin h2; PDBTitle: solution structure of bombinin h2 in dpc micelles 5 c2ap8A_ 23.8 46 PDB header:antibiotic Chain: A: PDB Molecule:bombinin h4; PDBTitle: solution structure of bombinin h4 in dpc micelles 6 d1jt6a1 20.9 6 Fold:DNA/RNA-binding 3-helical bundle Superfamily:Homeodomain-like Family:Tetracyclin repressor-like, N-terminal domain 7 d1rkta1 16.0 10 Fold:DNA/RNA-binding 3-helical bundle Superfamily:Homeodomain-like Family:Tetracyclin repressor-like, N-terminal domain 8 c1tt9B_ 15.3 9 PDB header:transferase, lyase Chain: B: PDB Molecule:formimidoyltransferase-cyclodeaminase PDBTitle: structure of the bifunctional and golgi associated2 formiminotransferase cyclodeaminase octamer 9 d1vi0a1 13.7 11 Fold:DNA/RNA-binding 3-helical bundle Superfamily:Homeodomain-like Family:Tetracyclin repressor-like, N-terminal domain 10 d1q8da_ 8.8 67 Fold:GDNF receptor-like Superfamily:GDNF receptor-like Family:GDNF receptor-like 11 c2dg8D_ 8.1 11 PDB header:gene regulation Chain: D: PDB Molecule:putative tetr-family transcriptional regulatory protein; PDBTitle: crystal structure of the putative trasncriptional regulator sco75182 from streptomyces coelicolor a3(2) 12 d1zk8a1 8.1 16 Fold:DNA/RNA-binding 3-helical bundle Superfamily:Homeodomain-like Family:Tetracyclin repressor-like, N-terminal domain 13 d1pb6a1 7.5 13 Fold:DNA/RNA-binding 3-helical bundle Superfamily:Homeodomain-like Family:Tetracyclin repressor-like, N-terminal domain 14 c3s5rA_ 7.5 15 PDB header:dna binding protein Chain: A: PDB Molecule:transcriptional regulator tetr family; PDBTitle: crystal structure of a putative transcriptional regulator of the tetr2 family (syn_02108) from syntrophus aciditrophicus at 2.60 a3 resolution 15 d2fbqa1 7.0 16 Fold:DNA/RNA-binding 3-helical bundle Superfamily:Homeodomain-like Family:Tetracyclin repressor-like, N-terminal domain 16 c3gziA_ 6.9 11 PDB header:transcription Chain: A: PDB Molecule:transcriptional regulator, tetr family; PDBTitle: crystal structure of a transcriptional regulator of the tetr family2 (shew_3567) from shewanella loihica pv-4 at 2.05 a resolution 17 c2l4gA_ 6.7 45 PDB header:viral protein Chain: A: PDB Molecule:haemagglutinin; PDBTitle: influenza haemagglutinin fusion peptide mutant g13a 18 c1u9nA_ 6.4 16 PDB header:dna binding protein Chain: A: PDB Molecule:transcriptional repressor ethr; PDBTitle: crystal structure of the transcriptional regulator ethr in2 a ligand bound conformation opens therapeutic perspectives3 against tuberculosis and leprosy 19 c2v5eA_ 6.2 50 PDB header:receptor/glycoprotein complex Chain: A: PDB Molecule:gdnf family receptor alpha-1; PDBTitle: the structure of the gdnf\:coreceptor complex\: insights2 into ret signalling and heparin binding. 20 c3fubA_ 6.2 50 PDB header:hormone Chain: A: PDB Molecule:gdnf family receptor alpha-1; PDBTitle: crystal structure of gdnf-gfralpha1 complex 21 c2dciA_ not modelled 6.2 45 PDB header:viral protein Chain: A: PDB Molecule:hemagglutinin; PDBTitle: nmr structure of influenza ha fusion peptide mutant w14a in2 dpc in ph5 22 c2oerA_ not modelled 5.7 13 PDB header:transcription Chain: A: PDB Molecule:probable transcriptional regulator; PDBTitle: probable transcriptional regulator from pseudomonas2 aeruginosa 23 c2hjdA_ not modelled 5.4 12 PDB header:signaling protein Chain: A: PDB Molecule:quorum-sensing antiactivator; PDBTitle: crystal structure of a second quorum sensing antiactivator tram2 from2 a. tumefaciens strain a6 24 d2o7ta1 not modelled 5.3 15 Fold:DNA/RNA-binding 3-helical bundle Superfamily:Homeodomain-like Family:Tetracyclin repressor-like, N-terminal domain 25 c3eupA_ not modelled 5.1 11 PDB header:transcription regulator Chain: A: PDB Molecule:transcriptional regulator, tetr family; PDBTitle: the crystal structure of the transcriptional regulator, tetr family2 from cytophaga hutchinsonii

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

Transmembrane helix prediction

Transmembrane helices have been predicted in your sequence to adopt the topology shown below

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0