Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	C	G	R	F	A	Q	S	Q	T	R	E	D	Y	L	A	L	L	A	E	D	I	E	R	D	I	P	Y	D	P	E	P	I	G	R	Y	N	V	A	P	G	T	K	V	L	L	L	S	E	R	D	E	H	L	H	L	D	P	V	F
Secondary structure
SS confidence
Disorder		?	?	?	?																								?		?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		W	G	Y	A	P	G	W	W	D	K	P	P	L	I	N	A	R	V	E	T	A	A	T	S	R	M	F	K	P	L	W	Q	H	G	R	A	I	C	F	A	D	G	W	F	E	W	K	K	E	G	D	K	K	Q	P	F	F	I	Y	R
Secondary structure
SS confidence
Disorder																																																	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		A	D	G	Q	P	I	F	M	A	A	I	G	S	T	P	F	E	R	G	D	E	A	E	G	F	L	I	V	T	A	A	A	D	Q	G	L	V	D	I	H	D	R	R	P	L	V	L	S	P	E	A	A	R	E	W	M	R	Q	E	I
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.
Sequence		S	G	K	E	A	S	E	I	A	A	S	G	C	V	P	A	N	Q	F	S	W	H	P	V	S	R	A	V	G	N	V	K	N	Q	G	A	E	L	I	Q	P	V
Secondary structure
SS confidence
Disorder																															?	?	?	?	?					?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 2icu chain B Region: 3 - 222 Aligned: 213 Modelled: 220 Confidence: 100.0% Identity: 98% PDB header:structural genomics, unknown function Chain: B: PDB Molecule:hypothetical protein yedk; PDBTitle: crystal structure of hypothetical protein yedk from escherichia coli Phyre2 PDB 2f20 chain A domain 1 Region: 2 - 221 Aligned: 215 Modelled: 219 Confidence: 100.0% Identity: 20% Fold: BB1717-like Superfamily: BB1717-like Family: BB1717-like Phyre2 PDB 2bdv chain A domain 1 Region: 2 - 217 Aligned: 208 Modelled: 216 Confidence: 100.0% Identity: 38% Fold: BB1717-like Superfamily: BB1717-like Family: BB1717-like Phyre2 PDB 1zn6 chain A domain 1 Region: 2 - 204 Aligned: 191 Modelled: 197 Confidence: 100.0% Identity: 20% Fold: BB1717-like Superfamily: BB1717-like Family: BB1717-like Phyre2 PDB 2aeg chain A Region: 2 - 208 Aligned: 197 Modelled: 199 Confidence: 100.0% Identity: 15% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:hypothetical protein agr_pat_140; PDBTitle: x-ray crystal structure of protein atu5096 from agrobacterium2 tumefaciens. northeast structural genomics consortium target atr63. Phyre2 PDB 2aeg chain A domain 1 Region: 2 - 208 Aligned: 197 Modelled: 207 Confidence: 100.0% Identity: 15% Fold: BB1717-like Superfamily: BB1717-like Family: BB1717-like Phyre2 PDB 3uks chain B Region: 159 - 176 Aligned: 18 Modelled: 18 Confidence: 36.4% Identity: 17% PDB header:hydrolase Chain: B: PDB Molecule:sedoheptulose-1,7 bisphosphatase, putative; PDBTitle: 1.85 angstrom crystal structure of putative sedoheptulose-1,72 bisphosphatase from toxoplasma gondii Phyre2 PDB 1d9q chain A Region: 159 - 175 Aligned: 17 Modelled: 17 Confidence: 34.1% Identity: 41% Fold: Carbohydrate phosphatase Superfamily: Carbohydrate phosphatase Family: Inositol monophosphatase/fructose-1,6-bisphosphatase-like Phyre2 PDB 1bk4 chain A Region: 159 - 176 Aligned: 18 Modelled: 18 Confidence: 34.0% Identity: 44% Fold: Carbohydrate phosphatase Superfamily: Carbohydrate phosphatase Family: Inositol monophosphatase/fructose-1,6-bisphosphatase-like Phyre2 PDB 1nuw chain A Region: 159 - 176 Aligned: 18 Modelled: 18 Confidence: 33.2% Identity: 50% Fold: Carbohydrate phosphatase Superfamily: Carbohydrate phosphatase Family: Inositol monophosphatase/fructose-1,6-bisphosphatase-like Phyre2 PDB 2gq1 chain A Region: 159 - 175 Aligned: 17 Modelled: 17 Confidence: 32.9% Identity: 18% PDB header:hydrolase Chain: A: PDB Molecule:fructose-1,6-bisphosphatase; PDBTitle: crystal structure of recombinant type i fructose-1,6-bisphosphatase2 from escherichia coli complexed with sulfate ions Phyre2 PDB 1fta chain A Region: 159 - 176 Aligned: 18 Modelled: 18 Confidence: 32.0% Identity: 44% Fold: Carbohydrate phosphatase Superfamily: Carbohydrate phosphatase Family: Inositol monophosphatase/fructose-1,6-bisphosphatase-like Phyre2 PDB 1spi chain A Region: 159 - 177 Aligned: 19 Modelled: 19 Confidence: 30.4% Identity: 37% Fold: Carbohydrate phosphatase Superfamily: Carbohydrate phosphatase Family: Inositol monophosphatase/fructose-1,6-bisphosphatase-like Phyre2 PDB 2fhy chain L Region: 159 - 176 Aligned: 18 Modelled: 18 Confidence: 27.9% Identity: 44% PDB header:hydrolase Chain: L: PDB Molecule:fructose-1,6-bisphosphatase 1; PDBTitle: structure of human liver fpbase complexed with a novel2 benzoxazole as allosteric inhibitor Phyre2 PDB 1v55 chain B Region: 160 - 176 Aligned: 17 Modelled: 17 Confidence: 22.0% Identity: 35% PDB header:oxidoreductase Chain: B: PDB Molecule:cytochrome c oxidase polypeptide ii; PDBTitle: bovine heart cytochrome c oxidase at the fully reduced state Phyre2 PDB 3dtu chain B domain 1 Region: 160 - 176 Aligned: 17 Modelled: 17 Confidence: 17.3% Identity: 41% Fold: Cupredoxin-like Superfamily: Cupredoxins Family: Periplasmic domain of cytochrome c oxidase subunit II Phyre2 PDB 2hew chain F domain 1 Region: 97 - 104 Aligned: 8 Modelled: 8 Confidence: 17.0% Identity: 25% Fold: TNF-like Superfamily: TNF-like Family: TNF-like Phyre2 PDB 2hew chain F Region: 97 - 104 Aligned: 8 Modelled: 8 Confidence: 17.0% Identity: 25% PDB header:cytokine Chain: F: PDB Molecule:tumor necrosis factor ligand superfamily member 4; PDBTitle: the x-ray crystal structure of murine ox40l Phyre2 PDB 2yqp chain A Region: 1 - 6 Aligned: 6 Modelled: 6 Confidence: 16.9% Identity: 50% PDB header:gene regulation, hydrolase Chain: A: PDB Molecule:probable atp-dependent rna helicase ddx59; PDBTitle: solution structure of the zf-hit domain in dead (asp-glu-2 ala-asp) box polypeptide 59 Phyre2 PDB 3ehb chain B domain 1 Region: 160 - 176 Aligned: 17 Modelled: 17 Confidence: 15.9% Identity: 29% Fold: Cupredoxin-like Superfamily: Cupredoxins Family: Periplasmic domain of cytochrome c oxidase subunit II Phyre2 1 c2icuB_ 2 d2f20a1 3 d2bdva1 4 d1zn6a1 5 c2aegA_ 6 d2aega1 7 c3uksB_ 8 d1d9qa_ 9 d1bk4a_ 10 d1nuwa_ 11 c2gq1A_ 12 d1ftaa_ 13 d1spia_ 14 c2fhyL_ 15 c1v55B_ 16 d3dtub1 17 d2hewf1 18 c2hewF_ 19 c2yqpA_ 20 d3ehbb1 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 c2icuB_ 100.0 98 PDB header:structural genomics, unknown function Chain: B: PDB Molecule:hypothetical protein yedk; PDBTitle: crystal structure of hypothetical protein yedk from escherichia coli 2 d2f20a1 100.0 20 Fold:BB1717-like Superfamily:BB1717-like Family:BB1717-like 3 d2bdva1 100.0 38 Fold:BB1717-like Superfamily:BB1717-like Family:BB1717-like 4 d1zn6a1 100.0 20 Fold:BB1717-like Superfamily:BB1717-like Family:BB1717-like 5 c2aegA_ 100.0 15 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:hypothetical protein agr_pat_140; PDBTitle: x-ray crystal structure of protein atu5096 from agrobacterium2 tumefaciens. northeast structural genomics consortium target atr63. 6 d2aega1 100.0 15 Fold:BB1717-like Superfamily:BB1717-like Family:BB1717-like 7 c3uksB_ 36.4 17 PDB header:hydrolase Chain: B: PDB Molecule:sedoheptulose-1,7 bisphosphatase, putative; PDBTitle: 1.85 angstrom crystal structure of putative sedoheptulose-1,72 bisphosphatase from toxoplasma gondii 8 d1d9qa_ 34.1 41 Fold:Carbohydrate phosphatase Superfamily:Carbohydrate phosphatase Family:Inositol monophosphatase/fructose-1,6-bisphosphatase-like 9 d1bk4a_ 34.0 44 Fold:Carbohydrate phosphatase Superfamily:Carbohydrate phosphatase Family:Inositol monophosphatase/fructose-1,6-bisphosphatase-like 10 d1nuwa_ 33.2 50 Fold:Carbohydrate phosphatase Superfamily:Carbohydrate phosphatase Family:Inositol monophosphatase/fructose-1,6-bisphosphatase-like 11 c2gq1A_ 32.9 18 PDB header:hydrolase Chain: A: PDB Molecule:fructose-1,6-bisphosphatase; PDBTitle: crystal structure of recombinant type i fructose-1,6-bisphosphatase2 from escherichia coli complexed with sulfate ions 12 d1ftaa_ 32.0 44 Fold:Carbohydrate phosphatase Superfamily:Carbohydrate phosphatase Family:Inositol monophosphatase/fructose-1,6-bisphosphatase-like 13 d1spia_ 30.4 37 Fold:Carbohydrate phosphatase Superfamily:Carbohydrate phosphatase Family:Inositol monophosphatase/fructose-1,6-bisphosphatase-like 14 c2fhyL_ 27.9 44 PDB header:hydrolase Chain: L: PDB Molecule:fructose-1,6-bisphosphatase 1; PDBTitle: structure of human liver fpbase complexed with a novel2 benzoxazole as allosteric inhibitor 15 c1v55B_ 22.0 35 PDB header:oxidoreductase Chain: B: PDB Molecule:cytochrome c oxidase polypeptide ii; PDBTitle: bovine heart cytochrome c oxidase at the fully reduced state 16 d3dtub1 17.3 41 Fold:Cupredoxin-like Superfamily:Cupredoxins Family:Periplasmic domain of cytochrome c oxidase subunit II 17 d2hewf1 17.0 25 Fold:TNF-like Superfamily:TNF-like Family:TNF-like 18 c2hewF_ 17.0 25 PDB header:cytokine Chain: F: PDB Molecule:tumor necrosis factor ligand superfamily member 4; PDBTitle: the x-ray crystal structure of murine ox40l 19 c2yqpA_ 16.9 50 PDB header:gene regulation, hydrolase Chain: A: PDB Molecule:probable atp-dependent rna helicase ddx59; PDBTitle: solution structure of the zf-hit domain in dead (asp-glu-2 ala-asp) box polypeptide 59 20 d3ehbb1 15.9 29 Fold:Cupredoxin-like Superfamily:Cupredoxins Family:Periplasmic domain of cytochrome c oxidase subunit II 21 d2hevf1 not modelled 14.6 38 Fold:TNF-like Superfamily:TNF-like Family:TNF-like 22 c2dhiA_ not modelled 14.4 11 PDB header:signaling protein Chain: A: PDB Molecule:pleckstrin homology domain-containing family b PDBTitle: solution structure of the ph domain of evectin-2 from mouse 23 c1m57H_ not modelled 14.2 35 PDB header:oxidoreductase Chain: H: PDB Molecule:cytochrome c oxidase; PDBTitle: structure of cytochrome c oxidase from rhodobacter2 sphaeroides (eq(i-286) mutant)) 24 c1ar1B_ not modelled 13.7 29 PDB header:complex (oxidoreductase/antibody) Chain: B: PDB Molecule:cytochrome c oxidase; PDBTitle: structure at 2.7 angstrom resolution of the paracoccus2 denitrificans two-subunit cytochrome c oxidase complexed3 with an antibody fv fragment 25 c1uijA_ not modelled 12.8 12 PDB header:sugar binding protein Chain: A: PDB Molecule:beta subunit of beta conglycinin; PDBTitle: crystal structure of soybean beta-conglycinin beta2 homotrimer (i122m/k124w) 26 d1v54b1 not modelled 11.5 24 Fold:Cupredoxin-like Superfamily:Cupredoxins Family:Periplasmic domain of cytochrome c oxidase subunit II 27 c3c3vA_ not modelled 10.9 10 PDB header:allergen Chain: A: PDB Molecule:arachin arah3 isoform; PDBTitle: crystal structure of peanut major allergen ara h 3 28 c1fftG_ not modelled 9.4 12 PDB header:oxidoreductase Chain: G: PDB Molecule:ubiquinol oxidase; PDBTitle: the structure of ubiquinol oxidase from escherichia coli 29 c2b3gB_ not modelled 9.0 42 PDB header:replication Chain: B: PDB Molecule:cellular tumor antigen p53; PDBTitle: p53n (fragment 33-60) bound to rpa70n 30 d2k49a2 not modelled 8.7 20 Fold:YegP-like Superfamily:YegP-like Family:YegP-like 31 c3kscD_ not modelled 8.1 8 PDB header:plant protein Chain: D: PDB Molecule:lega class; PDBTitle: crystal structure of pea prolegumin, an 11s seed globulin2 from pisum sativum l. 32 d2k8ea1 not modelled 8.1 26 Fold:YegP-like Superfamily:YegP-like Family:YegP-like 33 c1jsuC_ not modelled 8.0 27 PDB header:complex (transferase/cyclin/inhibitor) Chain: C: PDB Molecule:p27; PDBTitle: p27(kip1)/cyclin a/cdk2 complex 34 d2gysa1 not modelled 7.8 9 Fold:Immunoglobulin-like beta-sandwich Superfamily:Fibronectin type III Family:Fibronectin type III 35 c2l14B_ not modelled 7.6 26 PDB header:protein binding Chain: B: PDB Molecule:cellular tumor antigen p53; PDBTitle: structure of cbp nuclear coactivator binding domain in complex with2 p53 tad 36 c2k8eA_ not modelled 7.5 30 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:upf0339 protein yegp; PDBTitle: solution nmr structure of protein of unknown function yegp from e.2 coli. ontario center for structural proteomics target ec0640_1_1233 northeast structural genomics consortium target et102. 37 c1t3bA_ not modelled 7.5 11 PDB header:isomerase Chain: A: PDB Molecule:thiol:disulfide interchange protein dsbc; PDBTitle: x-ray structure of dsbc from haemophilus influenzae 38 c2gs0B_ not modelled 7.3 29 PDB header:transcription Chain: B: PDB Molecule:cellular tumor antigen p53; PDBTitle: nmr structure of the complex between the ph domain of the2 tfb1 subunit from tfiih and the activation domain of p53 39 c3rbbA_ not modelled 6.9 44 PDB header:viral protein, protein binding Chain: A: PDB Molecule:protein nef; PDBTitle: hiv-1 nef protein in complex with engineered hck sh3 domain 40 d1cyxa_ not modelled 6.6 18 Fold:Cupredoxin-like Superfamily:Cupredoxins Family:Periplasmic domain of cytochrome c oxidase subunit II 41 c1cyxA_ not modelled 6.6 18 PDB header:electron transport Chain: A: PDB Molecule:cyoa; PDBTitle: quinol oxidase (periplasmic fragment of subunit ii with2 engineered cu-a binding site)(cyoa) 42 c3qacA_ not modelled 5.6 10 PDB header:plant protein Chain: A: PDB Molecule:11s globulin seed storage protein; PDBTitle: structure of amaranth 11s proglobulin seed storage protein from2 amaranthus hypochondriacus l. 43 d1kr4a_ not modelled 5.6 23 Fold:Ferredoxin-like Superfamily:GlnB-like Family:Divalent ion tolerance proteins CutA (CutA1) 44 d2k57a1 not modelled 5.5 29 Fold:Sm-like fold Superfamily:Sm-like ribonucleoproteins Family:YgdI/YgdR-like 45 c2b7fD_ not modelled 5.4 25 PDB header:hydrolase/hydrolase inhibitor Chain: D: PDB Molecule:htlv protease; PDBTitle: crystal structure of human t-cell leukemia virus protease, a novel2 target for anti-cancer design 46 d1f0ya1 not modelled 5.4 29 Fold:6-phosphogluconate dehydrogenase C-terminal domain-like Superfamily:6-phosphogluconate dehydrogenase C-terminal domain-like Family:HCDH C-domain-like 47 d1p1la_ not modelled 5.3 23 Fold:Ferredoxin-like Superfamily:GlnB-like Family:Divalent ion tolerance proteins CutA (CutA1)

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

---

Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0