Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	A	E	H	L	M	S	D	V	P	F	W	Q	S	K	T	L	D	E	M	S	D	A	E	W	E	S	L	C	D	G	C	G	Q	C	C	L	H	K	L	M	D	E	D	T	D	E	I	Y	F	T	N	V	A	C	R	Q	L	N	I
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?		?										?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		K	T	C	Q	C	R	N	Y	E	R	R	F	E	F	E	P	D	C	I	K	L	T	R	E	N	L	P	T	F	E	W	L	P	M	T	C	A	Y	R	L	L	A	E	G	K	D	L	P	A	W	H	P	L	L	T	G	S	K	A	A
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.
Sequence		M	H	G	E	R	I	S	V	R	H	I	A	V	K	E	S	E	V	I	D	W	Q	D	H	I	L	N	K	P	D	W	A	Q
Secondary structure
SS confidence
Disorder					?																									?	?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 3bk7 chain A Region: 26 - 35 Aligned: 10 Modelled: 10 Confidence: 30.9% Identity: 60% PDB header:hydrolyase/translation Chain: A: PDB Molecule:abc transporter atp-binding protein; PDBTitle: structure of the complete abce1/rnaase-l inhibitor protein2 from pyrococcus abysii Phyre2 PDB 2aby chain A Region: 98 - 112 Aligned: 15 Modelled: 15 Confidence: 17.5% Identity: 40% PDB header:unknown function Chain: A: PDB Molecule:hypothetical protein ta0743; PDBTitle: solution structure of ta0743 from thermoplasma acidophilum Phyre2 PDB 2hlw chain A Region: 59 - 126 Aligned: 68 Modelled: 68 Confidence: 17.1% Identity: 16% PDB header:ligase, signaling protein Chain: A: PDB Molecule:ubiquitin-conjugating enzyme e2 variant 1; PDBTitle: solution structure of the human ubiquitin-conjugating2 enzyme variant uev1a Phyre2 PDB 1gth chain D Region: 26 - 56 Aligned: 31 Modelled: 31 Confidence: 15.6% Identity: 23% PDB header:oxidoreductase Chain: D: PDB Molecule:dihydropyrimidine dehydrogenase; PDBTitle: dihydropyrimidine dehydrogenase (dpd) from pig, ternary2 complex with nadph and 5-iodouracil Phyre2 PDB 2kvo chain A Region: 40 - 59 Aligned: 19 Modelled: 20 Confidence: 15.4% Identity: 32% PDB header:photosynthesis Chain: A: PDB Molecule:photosystem ii reaction center psb28 protein; PDBTitle: solution nmr structure of photosystem ii reaction center psb28 protein2 from synechocystis sp.(strain pcc 6803), northeast structural3 genomics consortium target sgr171 Phyre2 PDB 2gks chain B Region: 94 - 111 Aligned: 18 Modelled: 18 Confidence: 13.9% Identity: 28% PDB header:transferase Chain: B: PDB Molecule:bifunctional sat/aps kinase; PDBTitle: crystal structure of the bi-functional atp sulfurylase-aps kinase from2 aquifex aeolicus, a chemolithotrophic thermophile Phyre2 PDB 3gat chain A Region: 6 - 34 Aligned: 22 Modelled: 29 Confidence: 12.7% Identity: 23% Fold: Glucocorticoid receptor-like (DNA-binding domain) Superfamily: Glucocorticoid receptor-like (DNA-binding domain) Family: Erythroid transcription factor GATA-1 Phyre2 PDB 3u1x chain A Region: 80 - 118 Aligned: 31 Modelled: 39 Confidence: 10.7% Identity: 39% PDB header:hydrolase Chain: A: PDB Molecule:putative glycosyl hydrolase; PDBTitle: crystal structure of a putative glycosyl hydrolase (bdi_1869) from2 parabacteroides distasonis atcc 8503 at 1.70 a resolution Phyre2 PDB 2bs2 chain B domain 1 Region: 7 - 35 Aligned: 29 Modelled: 29 Confidence: 9.8% Identity: 24% Fold: Globin-like Superfamily: alpha-helical ferredoxin Family: Fumarate reductase/Succinate dehydogenase iron-sulfur protein, C-terminal domain Phyre2 PDB 2ksl chain A Region: 18 - 33 Aligned: 16 Modelled: 16 Confidence: 9.8% Identity: 38% PDB header:toxin Chain: A: PDB Molecule:u1-agatoxin-ta1a; PDBTitle: structure of the insecticidal toxin taitx-1 Phyre2 PDB 1wso chain A Region: 61 - 69 Aligned: 9 Modelled: 9 Confidence: 9.4% Identity: 78% PDB header:neuropeptide Chain: A: PDB Molecule:orexin-a; PDBTitle: the solution structures of human orexin-a Phyre2 PDB 2kae chain A Region: 6 - 34 Aligned: 23 Modelled: 29 Confidence: 9.3% Identity: 17% PDB header:transcription/dna Chain: A: PDB Molecule:gata-type transcription factor; PDBTitle: data-driven model of med1:dna complex Phyre2 PDB 2gmh chain A Region: 26 - 35 Aligned: 10 Modelled: 10 Confidence: 9.1% Identity: 30% PDB header:oxidoreductase Chain: A: PDB Molecule:electron transfer flavoprotein-ubiquinone PDBTitle: structure of porcine electron transfer flavoprotein-2 ubiquinone oxidoreductase in complexed with ubiquinone Phyre2 PDB 1sj1 chain A Region: 26 - 35 Aligned: 10 Modelled: 10 Confidence: 9.0% Identity: 30% Fold: Ferredoxin-like Superfamily: 4Fe-4S ferredoxins Family: Single 4Fe-4S cluster ferredoxin Phyre2 PDB 2nsv chain A Region: 91 - 98 Aligned: 8 Modelled: 8 Confidence: 8.6% Identity: 63% PDB header:signaling protein Chain: A: PDB Molecule:mating pheromone en-1; PDBTitle: nmr solution structure of the pheromone en-1 Phyre2 PDB 3dnl chain G Region: 78 - 83 Aligned: 6 Modelled: 6 Confidence: 8.5% Identity: 67% PDB header:viral protein Chain: G: PDB Molecule:hiv-1 envelope glycoprotein gp120; PDBTitle: molecular structure for the hiv-1 gp120 trimer in the b12-2 bound state Phyre2 PDB 1nek chain B domain 1 Region: 20 - 35 Aligned: 16 Modelled: 16 Confidence: 8.4% Identity: 38% Fold: Globin-like Superfamily: alpha-helical ferredoxin Family: Fumarate reductase/Succinate dehydogenase iron-sulfur protein, C-terminal domain Phyre2 PDB 2rpv chain A Region: 92 - 107 Aligned: 16 Modelled: 16 Confidence: 8.3% Identity: 44% PDB header:immune system Chain: A: PDB Molecule:immunoglobulin g-binding protein g; PDBTitle: solution structure of gb1 with lbt probe Phyre2 PDB 3ivu chain B Region: 104 - 131 Aligned: 27 Modelled: 28 Confidence: 8.1% Identity: 22% PDB header:transferase Chain: B: PDB Molecule:homocitrate synthase, mitochondrial; PDBTitle: homocitrate synthase lys4 bound to 2-og Phyre2 PDB 1fxr chain A Region: 26 - 35 Aligned: 10 Modelled: 10 Confidence: 8.0% Identity: 30% Fold: Ferredoxin-like Superfamily: 4Fe-4S ferredoxins Family: Single 4Fe-4S cluster ferredoxin Phyre2 1 c3bk7A_ 2 c2abyA_ 3 c2hlwA_ 4 c1gthD_ 5 c2kvoA_ 6 c2gksB_ 7 d3gata_ 8 c3u1xA_ 9 d2bs2b1 10 c2kslA_ 11 c1wsoA_ 12 c2kaeA_ 13 c2gmhA_ 14 d1sj1a_ 15 c2nsvA_ 16 c3dnlG_ 17 d1nekb1 18 c2rpvA_ 19 c3ivuB_ 20 d1fxra_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 c3bk7A_ 30.9 60 PDB header:hydrolyase/translation Chain: A: PDB Molecule:abc transporter atp-binding protein; PDBTitle: structure of the complete abce1/rnaase-l inhibitor protein2 from pyrococcus abysii 2 c2abyA_ 17.5 40 PDB header:unknown function Chain: A: PDB Molecule:hypothetical protein ta0743; PDBTitle: solution structure of ta0743 from thermoplasma acidophilum 3 c2hlwA_ 17.1 16 PDB header:ligase, signaling protein Chain: A: PDB Molecule:ubiquitin-conjugating enzyme e2 variant 1; PDBTitle: solution structure of the human ubiquitin-conjugating2 enzyme variant uev1a 4 c1gthD_ 15.6 23 PDB header:oxidoreductase Chain: D: PDB Molecule:dihydropyrimidine dehydrogenase; PDBTitle: dihydropyrimidine dehydrogenase (dpd) from pig, ternary2 complex with nadph and 5-iodouracil 5 c2kvoA_ 15.4 32 PDB header:photosynthesis Chain: A: PDB Molecule:photosystem ii reaction center psb28 protein; PDBTitle: solution nmr structure of photosystem ii reaction center psb28 protein2 from synechocystis sp.(strain pcc 6803), northeast structural3 genomics consortium target sgr171 6 c2gksB_ 13.9 28 PDB header:transferase Chain: B: PDB Molecule:bifunctional sat/aps kinase; PDBTitle: crystal structure of the bi-functional atp sulfurylase-aps kinase from2 aquifex aeolicus, a chemolithotrophic thermophile 7 d3gata_ 12.7 23 Fold:Glucocorticoid receptor-like (DNA-binding domain) Superfamily:Glucocorticoid receptor-like (DNA-binding domain) Family:Erythroid transcription factor GATA-1 8 c3u1xA_ 10.7 39 PDB header:hydrolase Chain: A: PDB Molecule:putative glycosyl hydrolase; PDBTitle: crystal structure of a putative glycosyl hydrolase (bdi_1869) from2 parabacteroides distasonis atcc 8503 at 1.70 a resolution 9 d2bs2b1 9.8 24 Fold:Globin-like Superfamily:alpha-helical ferredoxin Family:Fumarate reductase/Succinate dehydogenase iron-sulfur protein, C-terminal domain 10 c2kslA_ 9.8 38 PDB header:toxin Chain: A: PDB Molecule:u1-agatoxin-ta1a; PDBTitle: structure of the insecticidal toxin taitx-1 11 c1wsoA_ 9.4 78 PDB header:neuropeptide Chain: A: PDB Molecule:orexin-a; PDBTitle: the solution structures of human orexin-a 12 c2kaeA_ 9.3 17 PDB header:transcription/dna Chain: A: PDB Molecule:gata-type transcription factor; PDBTitle: data-driven model of med1:dna complex 13 c2gmhA_ 9.1 30 PDB header:oxidoreductase Chain: A: PDB Molecule:electron transfer flavoprotein-ubiquinone PDBTitle: structure of porcine electron transfer flavoprotein-2 ubiquinone oxidoreductase in complexed with ubiquinone 14 d1sj1a_ 9.0 30 Fold:Ferredoxin-like Superfamily:4Fe-4S ferredoxins Family:Single 4Fe-4S cluster ferredoxin 15 c2nsvA_ 8.6 63 PDB header:signaling protein Chain: A: PDB Molecule:mating pheromone en-1; PDBTitle: nmr solution structure of the pheromone en-1 16 c3dnlG_ 8.5 67 PDB header:viral protein Chain: G: PDB Molecule:hiv-1 envelope glycoprotein gp120; PDBTitle: molecular structure for the hiv-1 gp120 trimer in the b12-2 bound state 17 d1nekb1 8.4 38 Fold:Globin-like Superfamily:alpha-helical ferredoxin Family:Fumarate reductase/Succinate dehydogenase iron-sulfur protein, C-terminal domain 18 c2rpvA_ 8.3 44 PDB header:immune system Chain: A: PDB Molecule:immunoglobulin g-binding protein g; PDBTitle: solution structure of gb1 with lbt probe 19 c3ivuB_ 8.1 22 PDB header:transferase Chain: B: PDB Molecule:homocitrate synthase, mitochondrial; PDBTitle: homocitrate synthase lys4 bound to 2-og 20 d1fxra_ 8.0 30 Fold:Ferredoxin-like Superfamily:4Fe-4S ferredoxins Family:Single 4Fe-4S cluster ferredoxin 21 d1lwha1 not modelled 7.7 30 Fold:Glycosyl hydrolase domain Superfamily:Glycosyl hydrolase domain Family:alpha-Amylases, C-terminal beta-sheet domain 22 c2w3rG_ not modelled 7.7 21 PDB header:oxidoreductase Chain: G: PDB Molecule:xanthine dehydrogenase; PDBTitle: crystal structure of xanthine dehydrogenase (desulfo form)2 from rhodobacter capsulatus in complex with hypoxanthine 23 d1xera_ not modelled 7.6 44 Fold:Ferredoxin-like Superfamily:4Fe-4S ferredoxins Family:Archaeal ferredoxins 24 d1iw4a_ not modelled 7.4 32 Fold:Kazal-type serine protease inhibitors Superfamily:Kazal-type serine protease inhibitors Family:Ovomucoid domain III-like 25 d7fd1a_ not modelled 6.9 30 Fold:Ferredoxin-like Superfamily:4Fe-4S ferredoxins Family:7-Fe ferredoxin 26 d2cm5a1 not modelled 6.8 29 Fold:C2 domain-like Superfamily:C2 domain (Calcium/lipid-binding domain, CaLB) Family:Synaptotagmin-like (S variant) 27 d2vuti1 not modelled 6.7 27 Fold:Glucocorticoid receptor-like (DNA-binding domain) Superfamily:Glucocorticoid receptor-like (DNA-binding domain) Family:Erythroid transcription factor GATA-1 28 d2dlqa2 not modelled 6.6 45 Fold:beta-beta-alpha zinc fingers Superfamily:beta-beta-alpha zinc fingers Family:Classic zinc finger, C2H2 29 d5gata_ not modelled 6.5 27 Fold:Glucocorticoid receptor-like (DNA-binding domain) Superfamily:Glucocorticoid receptor-like (DNA-binding domain) Family:Erythroid transcription factor GATA-1 30 c1dwlA_ not modelled 6.5 40 PDB header:electron transfer Chain: A: PDB Molecule:ferredoxin i; PDBTitle: the ferredoxin-cytochrome complex using heteronuclear nmr2 and docking simulation 31 c1s6wA_ not modelled 6.4 83 PDB header:antibiotic Chain: A: PDB Molecule:hepcidin; PDBTitle: solution structure of hybrid white striped bass hepcidin 32 c2xglB_ not modelled 6.4 31 PDB header:antibiotic Chain: B: PDB Molecule:colicin-m immunity protein; PDBTitle: the x-ray structure of the escherichia coli colicin m immunity2 protein demonstrates the presence of a disulphide bridge, which is3 functionally essential 33 d1y0ja1 not modelled 6.0 27 Fold:Glucocorticoid receptor-like (DNA-binding domain) Superfamily:Glucocorticoid receptor-like (DNA-binding domain) Family:Erythroid transcription factor GATA-1 34 d1zata1 not modelled 5.9 17 Fold:L,D-transpeptidase catalytic domain-like Superfamily:L,D-transpeptidase catalytic domain-like Family:L,D-transpeptidase catalytic domain-like 35 c1t4vL_ not modelled 5.8 40 PDB header:hydrolase/hydrolase inhibitor Chain: L: PDB Molecule:prothrombin; PDBTitle: crystal structure analysis of a novel oxyguanidine bound to thrombin 36 c3c27A_ not modelled 5.8 40 PDB header:hydrolase/hydrolase inhibitor Chain: A: PDB Molecule:thrombin light chain; PDBTitle: cyanofluorophenylacetamides as orally efficacious thrombin inhibitors 37 c2r2mA_ not modelled 5.8 40 PDB header:hydrolase/hydrolase inhibitor Chain: A: PDB Molecule:thrombin light chain; PDBTitle: 2-(2-chloro-6-fluorophenyl)acetamides as potent thrombin inhibitors 38 c1t4uL_ not modelled 5.8 40 PDB header:hydrolase/hydrolase inhibitor Chain: L: PDB Molecule:prothrombin; PDBTitle: crystal structure analysis of a novel oxyguanidine bound to thrombin 39 d2fug91 not modelled 5.8 57 Fold:Ferredoxin-like Superfamily:4Fe-4S ferredoxins Family:Ferredoxin domains from multidomain proteins 40 c2fugG_ not modelled 5.8 57 PDB header:oxidoreductase Chain: G: PDB Molecule:nadh-quinone oxidoreductase chain 9; PDBTitle: crystal structure of the hydrophilic domain of respiratory complex i2 from thermus thermophilus 41 d1bc6a_ not modelled 5.8 40 Fold:Ferredoxin-like Superfamily:4Fe-4S ferredoxins Family:7-Fe ferredoxin 42 c1m8pB_ not modelled 5.7 38 PDB header:transferase Chain: B: PDB Molecule:sulfate adenylyltransferase; PDBTitle: crystal structure of p. chrysogenum atp sulfurylase in the t-state 43 c2c3yA_ not modelled 5.6 63 PDB header:oxidoreductase Chain: A: PDB Molecule:pyruvate-ferredoxin oxidoreductase; PDBTitle: crystal structure of the radical form of2 pyruvate:ferredoxin oxidoreductase from desulfovibrio3 africanus 44 d1xeba_ not modelled 5.6 21 Fold:Acyl-CoA N-acyltransferases (Nat) Superfamily:Acyl-CoA N-acyltransferases (Nat) Family:N-acetyl transferase, NAT 45 d2c42a5 not modelled 5.5 50 Fold:Ferredoxin-like Superfamily:4Fe-4S ferredoxins Family:Ferredoxin domains from multidomain proteins 46 d2it9a1 not modelled 5.5 29 Fold:ssDNA-binding transcriptional regulator domain Superfamily:ssDNA-binding transcriptional regulator domain Family:PMN2A0962/syc2379c-like 47 d1h98a_ not modelled 5.5 44 Fold:Ferredoxin-like Superfamily:4Fe-4S ferredoxins Family:7-Fe ferredoxin 48 d1gnfa_ not modelled 5.4 27 Fold:Glucocorticoid receptor-like (DNA-binding domain) Superfamily:Glucocorticoid receptor-like (DNA-binding domain) Family:Erythroid transcription factor GATA-1 49 c1p8vB_ not modelled 5.4 40 PDB header:membrane protein/hydrolase Chain: B: PDB Molecule:prothrombin; PDBTitle: crystal structure of the complex of platelet receptor gpib-alpha and2 alpha-thrombin at 2.6a 50 d1gtea5 not modelled 5.4 50 Fold:Ferredoxin-like Superfamily:4Fe-4S ferredoxins Family:Ferredoxin domains from multidomain proteins 51 d1iqza_ not modelled 5.2 20 Fold:Ferredoxin-like Superfamily:4Fe-4S ferredoxins Family:Single 4Fe-4S cluster ferredoxin 52 c1g8gB_ not modelled 5.2 38 PDB header:transferase Chain: B: PDB Molecule:sulfate adenylyltransferase; PDBTitle: atp sulfurylase from s. cerevisiae: the binary product complex with2 aps 53 d2nvna1 not modelled 5.1 29 Fold:ssDNA-binding transcriptional regulator domain Superfamily:ssDNA-binding transcriptional regulator domain Family:PMN2A0962/syc2379c-like 54 d1pcfa_ not modelled 5.1 18 Fold:ssDNA-binding transcriptional regulator domain Superfamily:ssDNA-binding transcriptional regulator domain Family:Transcriptional coactivator PC4 C-terminal domain 55 c3immC_ not modelled 5.1 31 PDB header:hydrolase Chain: C: PDB Molecule:putative secreted glycosylhydrolase; PDBTitle: crystal structure of putative glycosyl hydrolase (yp_001301887.1) from2 parabacteroides distasonis atcc 8503 at 2.00 a resolution 56 d1hfel2 not modelled 5.1 50 Fold:Ferredoxin-like Superfamily:4Fe-4S ferredoxins Family:Ferredoxin domains from multidomain proteins 57 c2kvfA_ not modelled 5.1 50 PDB header:transcription Chain: A: PDB Molecule:zinc finger and btb domain-containing protein 32; PDBTitle: structure of the three-cys2his2 domain of mouse testis zinc2 finger protein

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

---

Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0