Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	K	K	V	L	L	Q	N	H	P	G	S	E	K	Y	S	F	N	G	W	E	I	F	N	S	N	F	E	R	M	I	K	E	N	K	A	M	L	L	C	K	W	G	F	Y	L	T	C	V	V	A	V	M	F	V	F	A	A	I	T
Secondary structure
SS confidence
Disorder		?	?	?			?	?		?																																																?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		S	N	G	L	N	E	R	G	L	I	T	A	G	C	S	F	L	Y	L	L	I	M	M	G	L	I	V	R	A	G	F	K	A	K	K	E	Q	L	H	Y	Y	Q	A	K	G	I	E	P	L	S	I	E	K	L	Q	A	L	Q	L	I
Secondary structure
SS confidence
Disorder		?	?		?	?	?																																							?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		A	P	Y	R	F	Y	H	K	Q	W	S	E	T	L	E	F	W	P	R	K	P	E	P	G	K	D	T	F	Q	Y	H	V	L	P	F	D	S	I	D	I	I	S	K	R	R	E	S	L	E	D	Q	W	G	I	E	D	S	E	S	Y
Secondary structure
SS confidence
Disorder									?													?			?	?	?												?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		C	A	L	M	E	H	F	L	S	G	D	H	G	A	N	T	F	K	A	N	M	E	E	A	P	E	Q	V	I	A	L	L	N	K	F	A	V	F	P	S	D	Y	I	S	D	C	A	N	H	S	S	G	K	S	S	A	K	L	I	W
Secondary structure
SS confidence
Disorder														?						?			?	?	?	?	?																										?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		A	A	E	L	S	W	M	I	S	I	S	S	T	A	F	Q	N	G	T	I	E	E	E	L	A	W	H	Y	I	M	L	A	S	R	K	A	H	E	L	F	E	S	E	E	D	Y	Q	K	N	S	Q	M	G	F	L	Y	W	H	I	C
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.
Sequence		C	Y	R	R	K	L	T	D	A	E	L	E	A	C	Y	R	Y	D	K	Q	F	W	E	H	Y	S	K	K	C	R	W	P	I	R	N	V	P	W	G	A	S	S	V	K	Y	S
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?								?	?	?	?	?	?	?										?										?	?	?	?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 2w8a chain C Region: 36 - 115 Aligned: 80 Modelled: 80 Confidence: 64.8% Identity: 11% PDB header:membrane protein Chain: C: PDB Molecule:glycine betaine transporter betp; PDBTitle: crystal structure of the sodium-coupled glycine betaine2 symporter betp from corynebacterium glutamicum with bound3 substrate Phyre2 PDB 1jkv chain A Region: 159 - 206 Aligned: 48 Modelled: 48 Confidence: 22.1% Identity: 15% Fold: Ferritin-like Superfamily: Ferritin-like Family: Manganese catalase (T-catalase) Phyre2 PDB 1go5 chain A Region: 205 - 263 Aligned: 49 Modelled: 59 Confidence: 19.7% Identity: 8% Fold: RuvA C-terminal domain-like Superfamily: UBA-like Family: TAP-C domain-like Phyre2 PDB 2qwu chain B Region: 131 - 156 Aligned: 26 Modelled: 25 Confidence: 17.8% Identity: 35% PDB header:cell invasion Chain: B: PDB Molecule:intracellular growth locus, subunit c; PDBTitle: crystal structure of f. tularensis pathogenicity island2 protein c Phyre2 PDB 2cwl chain A domain 1 Region: 159 - 203 Aligned: 45 Modelled: 45 Confidence: 16.4% Identity: 18% Fold: Ferritin-like Superfamily: Ferritin-like Family: Manganese catalase (T-catalase) Phyre2 PDB 1oai chain A Region: 206 - 263 Aligned: 48 Modelled: 58 Confidence: 16.2% Identity: 8% Fold: RuvA C-terminal domain-like Superfamily: UBA-like Family: TAP-C domain-like Phyre2 PDB 1jog chain A Region: 249 - 281 Aligned: 33 Modelled: 33 Confidence: 15.3% Identity: 15% Fold: Four-helical up-and-down bundle Superfamily: Nucleotidyltransferase substrate binding subunit/domain Family: Family 1 bi-partite nucleotidyltransferase subunit Phyre2 PDB 1wty chain A Region: 249 - 281 Aligned: 33 Modelled: 33 Confidence: 14.7% Identity: 18% Fold: Four-helical up-and-down bundle Superfamily: Nucleotidyltransferase substrate binding subunit/domain Family: Family 1 bi-partite nucleotidyltransferase subunit Phyre2 PDB 3gkx chain B Region: 172 - 223 Aligned: 52 Modelled: 52 Confidence: 14.3% Identity: 2% PDB header:structural genomics, unknown function Chain: B: PDB Molecule:putative arsc family related protein; PDBTitle: crystal structure of putative arsc family related protein from2 bacteroides fragilis Phyre2 PDB 3cuq chain A Region: 94 - 134 Aligned: 27 Modelled: 27 Confidence: 14.2% Identity: 30% PDB header:protein transport Chain: A: PDB Molecule:vacuolar-sorting protein snf8; PDBTitle: integrated structural and functional model of the human escrt-ii2 complex Phyre2 PDB 2zme chain A Region: 94 - 137 Aligned: 30 Modelled: 30 Confidence: 13.5% Identity: 30% PDB header:protein transport Chain: A: PDB Molecule:vacuolar-sorting protein snf8; PDBTitle: integrated structural and functional model of the human escrt-ii2 complex Phyre2 PDB 2gyk chain B domain 1 Region: 271 - 292 Aligned: 22 Modelled: 22 Confidence: 13.2% Identity: 23% Fold: His-Me finger endonucleases Superfamily: His-Me finger endonucleases Family: HNH-motif Phyre2 PDB 1e8o chain A Region: 280 - 289 Aligned: 10 Modelled: 10 Confidence: 12.9% Identity: 10% Fold: Signal recognition particle alu RNA binding heterodimer, SRP9/14 Superfamily: Signal recognition particle alu RNA binding heterodimer, SRP9/14 Family: Signal recognition particle alu RNA binding heterodimer, SRP9/14 Phyre2 PDB 2rdd chain B Region: 74 - 99 Aligned: 26 Modelled: 26 Confidence: 12.4% Identity: 8% PDB header:membrane protein/transport protein Chain: B: PDB Molecule:upf0092 membrane protein yajc; PDBTitle: x-ray crystal structure of acrb in complex with a novel2 transmembrane helix. Phyre2 PDB 2job chain A Region: 251 - 270 Aligned: 20 Modelled: 20 Confidence: 12.1% Identity: 30% PDB header:lipid binding protein Chain: A: PDB Molecule:antilipopolysaccharide factor; PDBTitle: solution structure of an antilipopolysaccharide factor from2 shrimp and its possible lipid a binding site Phyre2 PDB 1914 chain A Region: 277 - 289 Aligned: 13 Modelled: 13 Confidence: 12.0% Identity: 23% PDB header:alu domain Chain: A: PDB Molecule:signal recognition particle 9/14 fusion protein; PDBTitle: signal recognition particle alu rna binding heterodimer, srp9/14 Phyre2 PDB 1zdb chain A Region: 94 - 117 Aligned: 24 Modelled: 24 Confidence: 11.9% Identity: 25% PDB header:igg binding domain Chain: A: PDB Molecule:mini protein a domain, z38; PDBTitle: phage-selected mini protein a domain, z38, nmr, minimized2 mean structure Phyre2 PDB 1wwp chain A Region: 249 - 281 Aligned: 33 Modelled: 33 Confidence: 11.7% Identity: 12% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:hypothetical protein ttha0636; PDBTitle: crystal structure of ttk003001694 from thermus thermophilus2 hb8 Phyre2 PDB 2rh2 chain A domain 1 Region: 9 - 19 Aligned: 11 Modelled: 11 Confidence: 10.6% Identity: 45% Fold: SH3-like barrel Superfamily: Electron transport accessory proteins Family: R67 dihydrofolate reductase Phyre2 PDB 1qqr chain A Region: 2 - 13 Aligned: 12 Modelled: 12 Confidence: 9.0% Identity: 42% Fold: beta-Grasp (ubiquitin-like) Superfamily: Staphylokinase/streptokinase Family: Staphylokinase/streptokinase Phyre2 1 c2w8aC_ 2 d1jkva_ 3 d1go5a_ 4 c2qwuB_ 5 d2cwla1 6 d1oaia_ 7 d1joga_ 8 d1wtya_ 9 c3gkxB_ 10 c3cuqA_ 11 c2zmeA_ 12 d2gykb1 13 d1e8oa_ 14 c2rddB_ 15 c2jobA_ 16 c1914A_ 17 c1zdbA_ 18 c1wwpA_ 19 d2rh2a1 20 d1qqra_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 c2w8aC_ 64.8 11 PDB header:membrane protein Chain: C: PDB Molecule:glycine betaine transporter betp; PDBTitle: crystal structure of the sodium-coupled glycine betaine2 symporter betp from corynebacterium glutamicum with bound3 substrate 2 d1jkva_ 22.1 15 Fold:Ferritin-like Superfamily:Ferritin-like Family:Manganese catalase (T-catalase) 3 d1go5a_ 19.7 8 Fold:RuvA C-terminal domain-like Superfamily:UBA-like Family:TAP-C domain-like 4 c2qwuB_ 17.8 35 PDB header:cell invasion Chain: B: PDB Molecule:intracellular growth locus, subunit c; PDBTitle: crystal structure of f. tularensis pathogenicity island2 protein c 5 d2cwla1 16.4 18 Fold:Ferritin-like Superfamily:Ferritin-like Family:Manganese catalase (T-catalase) 6 d1oaia_ 16.2 8 Fold:RuvA C-terminal domain-like Superfamily:UBA-like Family:TAP-C domain-like 7 d1joga_ 15.3 15 Fold:Four-helical up-and-down bundle Superfamily:Nucleotidyltransferase substrate binding subunit/domain Family:Family 1 bi-partite nucleotidyltransferase subunit 8 d1wtya_ 14.7 18 Fold:Four-helical up-and-down bundle Superfamily:Nucleotidyltransferase substrate binding subunit/domain Family:Family 1 bi-partite nucleotidyltransferase subunit 9 c3gkxB_ 14.3 2 PDB header:structural genomics, unknown function Chain: B: PDB Molecule:putative arsc family related protein; PDBTitle: crystal structure of putative arsc family related protein from2 bacteroides fragilis 10 c3cuqA_ 14.2 30 PDB header:protein transport Chain: A: PDB Molecule:vacuolar-sorting protein snf8; PDBTitle: integrated structural and functional model of the human escrt-ii2 complex 11 c2zmeA_ 13.5 30 PDB header:protein transport Chain: A: PDB Molecule:vacuolar-sorting protein snf8; PDBTitle: integrated structural and functional model of the human escrt-ii2 complex 12 d2gykb1 13.2 23 Fold:His-Me finger endonucleases Superfamily:His-Me finger endonucleases Family:HNH-motif 13 d1e8oa_ 12.9 10 Fold:Signal recognition particle alu RNA binding heterodimer, SRP9/14 Superfamily:Signal recognition particle alu RNA binding heterodimer, SRP9/14 Family:Signal recognition particle alu RNA binding heterodimer, SRP9/14 14 c2rddB_ 12.4 8 PDB header:membrane protein/transport protein Chain: B: PDB Molecule:upf0092 membrane protein yajc; PDBTitle: x-ray crystal structure of acrb in complex with a novel2 transmembrane helix. 15 c2jobA_ 12.1 30 PDB header:lipid binding protein Chain: A: PDB Molecule:antilipopolysaccharide factor; PDBTitle: solution structure of an antilipopolysaccharide factor from2 shrimp and its possible lipid a binding site 16 c1914A_ 12.0 23 PDB header:alu domain Chain: A: PDB Molecule:signal recognition particle 9/14 fusion protein; PDBTitle: signal recognition particle alu rna binding heterodimer, srp9/14 17 c1zdbA_ 11.9 25 PDB header:igg binding domain Chain: A: PDB Molecule:mini protein a domain, z38; PDBTitle: phage-selected mini protein a domain, z38, nmr, minimized2 mean structure 18 c1wwpA_ 11.7 12 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:hypothetical protein ttha0636; PDBTitle: crystal structure of ttk003001694 from thermus thermophilus2 hb8 19 d2rh2a1 10.6 45 Fold:SH3-like barrel Superfamily:Electron transport accessory proteins Family:R67 dihydrofolate reductase 20 d1qqra_ 9.0 42 Fold:beta-Grasp (ubiquitin-like) Superfamily:Staphylokinase/streptokinase Family:Staphylokinase/streptokinase 21 c3fz4A_ not modelled 8.6 2 PDB header:oxidoreductase Chain: A: PDB Molecule:putative arsenate reductase; PDBTitle: the crystal structure of a possible arsenate reductase from2 streptococcus mutans ua159 22 d1dd4d_ not modelled 8.4 27 Fold:Ribosomal protein L7/12, oligomerisation (N-terminal) domain Superfamily:Ribosomal protein L7/12, oligomerisation (N-terminal) domain Family:Ribosomal protein L7/12, oligomerisation (N-terminal) domain 23 d1f6va_ not modelled 8.2 20 Fold:C-terminal domain of B transposition protein Superfamily:C-terminal domain of B transposition protein Family:C-terminal domain of B transposition protein 24 c2vqcA_ not modelled 7.9 17 PDB header:dna-binding protein Chain: A: PDB Molecule:hypothetical 13.2 kda protein; PDBTitle: structure of a dna binding winged-helix protein, f-112,2 from sulfolobus spindle-shaped virus 1. 25 d2vqca1 not modelled 7.9 17 Fold:DNA/RNA-binding 3-helical bundle Superfamily:"Winged helix" DNA-binding domain Family:F112-like 26 c3kcvG_ not modelled 7.9 13 PDB header:transport protein Chain: G: PDB Molecule:probable formate transporter 1; PDBTitle: structure of formate channel 27 d1eexa_ not modelled 7.6 20 Fold:TIM beta/alpha-barrel Superfamily:Cobalamin (vitamin B12)-dependent enzymes Family:Diol dehydratase, alpha subunit 28 d1tz7a1 not modelled 7.6 19 Fold:TIM beta/alpha-barrel Superfamily:(Trans)glycosidases Family:Amylase, catalytic domain 29 d2p12a1 not modelled 7.5 17 Fold:FomD barrel-like Superfamily:FomD-like Family:FomD-like 30 d2jioa2 not modelled 7.4 13 Fold:Formate dehydrogenase/DMSO reductase, domains 1-3 Superfamily:Formate dehydrogenase/DMSO reductase, domains 1-3 Family:Formate dehydrogenase/DMSO reductase, domains 1-3 31 c3l78A_ not modelled 7.3 6 PDB header:transcription Chain: A: PDB Molecule:regulatory protein spx; PDBTitle: the crystal structure of smu.1142c from streptococcus mutans ua159 32 d1eswa_ not modelled 7.2 11 Fold:TIM beta/alpha-barrel Superfamily:(Trans)glycosidases Family:Amylase, catalytic domain 33 d1qusa_ not modelled 7.1 15 Fold:Lysozyme-like Superfamily:Lysozyme-like Family:Bacterial muramidase, catalytic domain 34 c3sokB_ not modelled 6.9 35 PDB header:cell adhesion Chain: B: PDB Molecule:fimbrial protein; PDBTitle: dichelobacter nodosus pilin fima 35 d2o8ia1 not modelled 6.9 21 Fold:UraD-like Superfamily:UraD-Like Family:UraD-like 36 d1914a1 not modelled 6.7 20 Fold:Signal recognition particle alu RNA binding heterodimer, SRP9/14 Superfamily:Signal recognition particle alu RNA binding heterodimer, SRP9/14 Family:Signal recognition particle alu RNA binding heterodimer, SRP9/14 37 d1oqwa_ not modelled 6.2 41 Fold:Pili subunits Superfamily:Pili subunits Family:Pilin 38 c2v5iA_ not modelled 6.2 16 PDB header:viral protein Chain: A: PDB Molecule:salmonella typhimurium db7155 bacteriophage det7 PDBTitle: structure of the receptor-binding protein of bacteriophage2 det7: a podoviral tailspike in a myovirus 39 c3klzE_ not modelled 6.2 12 PDB header:membrane protein Chain: E: PDB Molecule:putative formate transporter 1; PDBTitle: pentameric formate channel with formate bound 40 c1svfB_ not modelled 6.0 35 PDB header:viral protein Chain: B: PDB Molecule:protein (fusion glycoprotein); PDBTitle: paramyxovirus sv5 fusion protein core 41 d1x1na1 not modelled 6.0 15 Fold:TIM beta/alpha-barrel Superfamily:(Trans)glycosidases Family:Amylase, catalytic domain 42 d2fug34 not modelled 5.7 40 Fold:Ferredoxin-like Superfamily:4Fe-4S ferredoxins Family:Ferredoxin domains from multidomain proteins 43 c2p7vA_ not modelled 5.6 33 PDB header:transcription Chain: A: PDB Molecule:regulator of sigma d; PDBTitle: crystal structure of the escherichia coli regulator of sigma 70, rsd,2 in complex with sigma 70 domain 4 44 d2pila_ not modelled 5.6 29 Fold:Pili subunits Superfamily:Pili subunits Family:Pilin 45 d1pyta_ not modelled 5.4 16 Fold:Ferredoxin-like Superfamily:Protease propeptides/inhibitors Family:Pancreatic carboxypeptidase, activation domain 46 c1bhbA_ not modelled 5.3 24 PDB header:photoreceptor Chain: A: PDB Molecule:bacteriorhodopsin; PDBTitle: three-dimensional structure of (1-71) bacterioopsin2 solubilized in methanol-chloroform and sds micelles3 determined by 15n-1h heteronuclear nmr spectroscopy 47 c2k85A_ not modelled 5.2 21 PDB header:protein binding Chain: A: PDB Molecule:glucocorticoid receptor dna-binding factor 1; PDBTitle: p190-a rhogap ff1 domain 48 c3m8eA_ not modelled 5.1 19 PDB header:dna binding protein Chain: A: PDB Molecule:putative dna-binding protein; PDBTitle: protein structure of type iii plasmid segregation tubr 49 d2q37a1 not modelled 5.1 21 Fold:UraD-like Superfamily:UraD-Like Family:UraD-like 50 c2q37A_ not modelled 5.1 21 PDB header:plant protein, lyase Chain: A: PDB Molecule:ohcu decarboxylase; PDBTitle: crystal structure of ohcu decarboxylase in the presence of2 (s)-allantoin

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

Transmembrane helix prediction

Transmembrane helices have been predicted in your sequence to adopt the topology shown below

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0