Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	I	I	G	N	I	H	N	L	Q	P	W	L	P	Q	E	L	R	Q	A	I	E	H	I	K	A	H	V	T	A	E	T	P	K	G	K	H	D	I	E	G	N	R	L	F	Y	L	I	S	E	D	M	T	E	P	Y	E	A	R	R
Secondary structure
SS confidence
Disorder		?	?	?	?	?																																														?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		A	E	Y	H	A	R	Y	L	D	I	Q	I	V	L	K	G	Q	E	G	M	T	F	S	T	Q	P	A	G	A	P	D	T	D	W	L	A	D	K	D	I	A	F	L	P	E	G	V	D	E	K	T	V	I	L	N	E	G	D	F	V
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150
Sequence		V	F	Y	P	G	E	V	H	K	P	L	C	A	V	G	A	P	A	Q	V	R	K	A	V	V	K	M	L	M	A
Secondary structure
SS confidence
Disorder																														?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 1s4c chain A domain 1 Region: 1 - 135 Aligned: 134 Modelled: 135 Confidence: 100.0% Identity: 28% Fold: Double-stranded beta-helix Superfamily: Clavaminate synthase-like Family: YhcH-like Phyre2 PDB 1yfu chain A domain 1 Region: 22 - 130 Aligned: 85 Modelled: 88 Confidence: 85.4% Identity: 20% Fold: Double-stranded beta-helix Superfamily: RmlC-like cupins Family: 3-hydroxyanthranilic acid dioxygenase-like Phyre2 PDB 1v70 chain A Region: 39 - 148 Aligned: 81 Modelled: 85 Confidence: 76.9% Identity: 17% Fold: Double-stranded beta-helix Superfamily: RmlC-like cupins Family: TM1287-like Phyre2 PDB 3cew chain A Region: 63 - 148 Aligned: 59 Modelled: 61 Confidence: 59.9% Identity: 19% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:uncharacterized cupin protein; PDBTitle: crystal structure of a cupin protein (bf4112) from bacteroides2 fragilis. northeast structural genomics consortium target bfr205 Phyre2 PDB 2i45 chain C Region: 61 - 130 Aligned: 46 Modelled: 46 Confidence: 58.1% Identity: 22% PDB header:structural genomics, unknown function Chain: C: PDB Molecule:hypothetical protein; PDBTitle: crystal structure of protein nmb1881 from neisseria meningitidis Phyre2 PDB 1zvf chain A domain 1 Region: 22 - 130 Aligned: 88 Modelled: 92 Confidence: 41.8% Identity: 20% Fold: Double-stranded beta-helix Superfamily: RmlC-like cupins Family: 3-hydroxyanthranilic acid dioxygenase-like Phyre2 PDB 1o4t chain A Region: 45 - 149 Aligned: 78 Modelled: 80 Confidence: 41.2% Identity: 24% Fold: Double-stranded beta-helix Superfamily: RmlC-like cupins Family: TM1287-like Phyre2 PDB 1vj2 chain A Region: 40 - 146 Aligned: 79 Modelled: 82 Confidence: 35.2% Identity: 16% Fold: Double-stranded beta-helix Superfamily: RmlC-like cupins Family: TM1459-like Phyre2 PDB 2pa7 chain A domain 1 Region: 42 - 130 Aligned: 85 Modelled: 89 Confidence: 33.8% Identity: 13% Fold: Double-stranded beta-helix Superfamily: RmlC-like cupins Family: dTDP-sugar isomerase Phyre2 PDB 2arc chain A Region: 108 - 129 Aligned: 22 Modelled: 22 Confidence: 33.7% Identity: 32% Fold: Double-stranded beta-helix Superfamily: Regulatory protein AraC Family: Regulatory protein AraC Phyre2 PDB 2xxz chain A Region: 102 - 129 Aligned: 28 Modelled: 28 Confidence: 33.6% Identity: 21% PDB header:oxidoreductase Chain: A: PDB Molecule:lysine-specific demethylase 6b; PDBTitle: crystal structure of the human jmjd3 jumonji domain Phyre2 PDB 1j3p chain A Region: 59 - 129 Aligned: 51 Modelled: 51 Confidence: 32.8% Identity: 24% Fold: Double-stranded beta-helix Superfamily: RmlC-like cupins Family: Glucose-6-phosphate isomerase, GPI Phyre2 PDB 2xue chain B Region: 102 - 129 Aligned: 28 Modelled: 28 Confidence: 30.6% Identity: 21% PDB header:oxidoreductase Chain: B: PDB Molecule:lysine-specific demethylase 6b; PDBTitle: crystal structure of jmjd3 Phyre2 PDB 3d82 chain A Region: 58 - 130 Aligned: 48 Modelled: 48 Confidence: 30.4% Identity: 38% PDB header:metal binding protein Chain: A: PDB Molecule:cupin 2, conserved barrel domain protein; PDBTitle: crystal structure of a cupin-2 domain containing protein (sfri_3543)2 from shewanella frigidimarina ncimb 400 at 2.05 a resolution Phyre2 PDB 2ozj chain B Region: 39 - 129 Aligned: 63 Modelled: 66 Confidence: 27.7% Identity: 24% PDB header:unknown function Chain: B: PDB Molecule:cupin 2, conserved barrel; PDBTitle: crystal structure of a cupin superfamily protein (dsy2733) from2 desulfitobacterium hafniense dcb-2 at 1.60 a resolution Phyre2 PDB 2fqp chain D Region: 36 - 150 Aligned: 88 Modelled: 91 Confidence: 27.4% Identity: 13% PDB header:metal binding protein Chain: D: PDB Molecule:hypothetical protein bp2299; PDBTitle: crystal structure of a cupin domain (bp2299) from bordetella pertussis2 tohama i at 1.80 a resolution Phyre2 PDB 3kgz chain A Region: 36 - 144 Aligned: 83 Modelled: 84 Confidence: 23.5% Identity: 16% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:cupin 2 conserved barrel domain protein; PDBTitle: crystal structure of a cupin 2 conserved barrel domain protein from2 rhodopseudomonas palustris Phyre2 PDB 2oa2 chain A Region: 46 - 122 Aligned: 77 Modelled: 77 Confidence: 22.7% Identity: 10% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:bh2720 protein; PDBTitle: crystal structure of bh2720 (10175341) from bacillus halodurans at2 1.41 a resolution Phyre2 PDB 1a6q chain A domain 1 Region: 17 - 35 Aligned: 19 Modelled: 19 Confidence: 21.0% Identity: 16% Fold: Another 3-helical bundle Superfamily: Protein serine/threonine phosphatase 2C, C-terminal domain Family: Protein serine/threonine phosphatase 2C, C-terminal domain Phyre2 PDB 1y9q chain A domain 2 Region: 108 - 145 Aligned: 36 Modelled: 38 Confidence: 15.8% Identity: 25% Fold: Double-stranded beta-helix Superfamily: RmlC-like cupins Family: Probable transcriptional regulator VC1968, C-terminal domain Phyre2 1 d1s4ca1 2 d1yfua1 3 d1v70a_ 4 c3cewA_ 5 c2i45C_ 6 d1zvfa1 7 d1o4ta_ 8 d1vj2a_ 9 d2pa7a1 10 d2arca_ 11 c2xxzA_ 12 d1j3pa_ 13 c2xueB_ 14 c3d82A_ 15 c2ozjB_ 16 c2fqpD_ 17 c3kgzA_ 18 c2oa2A_ 19 d1a6qa1 20 d1y9qa2 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 d1s4ca1 100.0 28 Fold:Double-stranded beta-helix Superfamily:Clavaminate synthase-like Family:YhcH-like 2 d1yfua1 85.4 20 Fold:Double-stranded beta-helix Superfamily:RmlC-like cupins Family:3-hydroxyanthranilic acid dioxygenase-like 3 d1v70a_ 76.9 17 Fold:Double-stranded beta-helix Superfamily:RmlC-like cupins Family:TM1287-like 4 c3cewA_ 59.9 19 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:uncharacterized cupin protein; PDBTitle: crystal structure of a cupin protein (bf4112) from bacteroides2 fragilis. northeast structural genomics consortium target bfr205 5 c2i45C_ 58.1 22 PDB header:structural genomics, unknown function Chain: C: PDB Molecule:hypothetical protein; PDBTitle: crystal structure of protein nmb1881 from neisseria meningitidis 6 d1zvfa1 41.8 20 Fold:Double-stranded beta-helix Superfamily:RmlC-like cupins Family:3-hydroxyanthranilic acid dioxygenase-like 7 d1o4ta_ 41.2 24 Fold:Double-stranded beta-helix Superfamily:RmlC-like cupins Family:TM1287-like 8 d1vj2a_ 35.2 16 Fold:Double-stranded beta-helix Superfamily:RmlC-like cupins Family:TM1459-like 9 d2pa7a1 33.8 13 Fold:Double-stranded beta-helix Superfamily:RmlC-like cupins Family:dTDP-sugar isomerase 10 d2arca_ 33.7 32 Fold:Double-stranded beta-helix Superfamily:Regulatory protein AraC Family:Regulatory protein AraC 11 c2xxzA_ 33.6 21 PDB header:oxidoreductase Chain: A: PDB Molecule:lysine-specific demethylase 6b; PDBTitle: crystal structure of the human jmjd3 jumonji domain 12 d1j3pa_ 32.8 24 Fold:Double-stranded beta-helix Superfamily:RmlC-like cupins Family:Glucose-6-phosphate isomerase, GPI 13 c2xueB_ 30.6 21 PDB header:oxidoreductase Chain: B: PDB Molecule:lysine-specific demethylase 6b; PDBTitle: crystal structure of jmjd3 14 c3d82A_ 30.4 38 PDB header:metal binding protein Chain: A: PDB Molecule:cupin 2, conserved barrel domain protein; PDBTitle: crystal structure of a cupin-2 domain containing protein (sfri_3543)2 from shewanella frigidimarina ncimb 400 at 2.05 a resolution 15 c2ozjB_ 27.7 24 PDB header:unknown function Chain: B: PDB Molecule:cupin 2, conserved barrel; PDBTitle: crystal structure of a cupin superfamily protein (dsy2733) from2 desulfitobacterium hafniense dcb-2 at 1.60 a resolution 16 c2fqpD_ 27.4 13 PDB header:metal binding protein Chain: D: PDB Molecule:hypothetical protein bp2299; PDBTitle: crystal structure of a cupin domain (bp2299) from bordetella pertussis2 tohama i at 1.80 a resolution 17 c3kgzA_ 23.5 16 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:cupin 2 conserved barrel domain protein; PDBTitle: crystal structure of a cupin 2 conserved barrel domain protein from2 rhodopseudomonas palustris 18 c2oa2A_ 22.7 10 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:bh2720 protein; PDBTitle: crystal structure of bh2720 (10175341) from bacillus halodurans at2 1.41 a resolution 19 d1a6qa1 21.0 16 Fold:Another 3-helical bundle Superfamily:Protein serine/threonine phosphatase 2C, C-terminal domain Family:Protein serine/threonine phosphatase 2C, C-terminal domain 20 d1y9qa2 15.8 25 Fold:Double-stranded beta-helix Superfamily:RmlC-like cupins Family:Probable transcriptional regulator VC1968, C-terminal domain 21 c2w2iC_ not modelled 14.6 24 PDB header:oxidoreductase Chain: C: PDB Molecule:2-oxoglutarate oxygenase; PDBTitle: crystal structure of the human 2-oxoglutarate oxygenase2 loc390245 22 c2hdlA_ not modelled 13.6 24 PDB header:cytokine Chain: A: PDB Molecule:small inducible cytokine b14; PDBTitle: solution structure of brak/cxcl14 23 d3bb6a1 not modelled 13.1 24 Fold:Double-stranded beta-helix Superfamily:Clavaminate synthase-like Family:TehB-like 24 c2os2A_ not modelled 13.1 23 PDB header:oxidoreductase Chain: A: PDB Molecule:jmjc domain-containing histone demethylation protein 3a; PDBTitle: crystal structure of jmjd2a complexed with histone h3 peptide2 trimethylated at lys36 25 c2q8eB_ not modelled 13.0 24 PDB header:oxidoreductase Chain: B: PDB Molecule:jmjc domain-containing histone demethylation PDBTitle: specificity and mechanism of jmjd2a, a trimethyllysine-2 specific histone demethylase 26 c3opwA_ not modelled 13.0 28 PDB header:oxidoreductase Chain: A: PDB Molecule:dna damage-responsive transcriptional repressor rph1; PDBTitle: crystal structure of the rph1 catalytic core 27 d3dl3a1 not modelled 12.9 27 Fold:Double-stranded beta-helix Superfamily:Clavaminate synthase-like Family:TehB-like 28 c3ibmB_ not modelled 11.5 23 PDB header:structural genomics, unknown function Chain: B: PDB Molecule:cupin 2, conserved barrel domain protein; PDBTitle: crystal structure of cupin 2 domain-containing protein hhal_0468 from2 halorhodospira halophila 29 c3ebrA_ not modelled 11.2 16 PDB header:unknown function Chain: A: PDB Molecule:uncharacterized rmlc-like cupin; PDBTitle: crystal structure of an rmlc-like cupin protein (reut_a0381) from2 ralstonia eutropha jmp134 at 2.60 a resolution 30 c3fjsC_ not modelled 10.8 27 PDB header:biosynthetic protein Chain: C: PDB Molecule:uncharacterized protein with rmlc-like cupin fold; PDBTitle: crystal structure of a putative biosynthetic protein with rmlc-like2 cupin fold (reut_b4087) from ralstonia eutropha jmp134 at 1.90 a3 resolution 31 d2es7a1 not modelled 10.3 21 Fold:Thioredoxin fold Superfamily:Thioredoxin-like Family:HyaE-like 32 c2pyhB_ not modelled 10.1 26 PDB header:isomerase Chain: B: PDB Molecule:poly(beta-d-mannuronate) c5 epimerase 4; PDBTitle: azotobacter vinelandii mannuronan c-5 epimerase alge4 a-module2 complexed with mannuronan trisaccharide 33 c3h8uA_ not modelled 9.9 22 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:uncharacterized conserved protein with double-stranded PDBTitle: crystal structure of uncharacterized conserved protein with double-2 stranded beta-helix domain (yp_001338853.1) from klebsiella3 pneumoniae subsp. pneumoniae mgh 78578 at 1.80 a resolution 34 c2r32A_ not modelled 9.8 7 PDB header:immune system Chain: A: PDB Molecule:gcn4-pii/tumor necrosis factor ligand PDBTitle: crystal structure of human gitrl variant 35 c2bx6A_ not modelled 9.6 24 PDB header:transduction protein Chain: A: PDB Molecule:xrp2 protein; PDBTitle: crystal structure of the human retinitis pigmentosa2 protein 2 (rp2) 36 c3l2hD_ not modelled 9.3 18 PDB header:isomerase Chain: D: PDB Molecule:putative sugar phosphate isomerase; PDBTitle: crystal structure of putative sugar phosphate isomerase (afe_0303)2 from acidithiobacillus ferrooxidans atcc 23270 at 1.85 a resolution 37 c2gu9B_ not modelled 9.2 15 PDB header:immune system Chain: B: PDB Molecule:tetracenomycin polyketide synthesis protein; PDBTitle: crystal structure of xc5357 from xanthomonas campestris: a2 putative tetracenomycin polyketide synthesis protein3 adopting a novel cupin subfamily structure 38 c2oziA_ not modelled 8.8 18 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:hypothetical protein rpa4178; PDBTitle: structural genomics, the crystal structure of a putative2 protein rpa4178 from rhodopseudomonas palustris cga009 39 c3ht2A_ not modelled 8.6 28 PDB header:lyase Chain: A: PDB Molecule:remf protein; PDBTitle: zink containing polyketide cyclase remf from streptomyces2 resistomycificus 40 c2q30C_ not modelled 7.7 17 PDB header:unknown function Chain: C: PDB Molecule:uncharacterized protein; PDBTitle: crystal structure of a rmlc-like cupin protein (dde_2303) from2 desulfovibrio desulfuricans subsp. at 1.94 a resolution 41 c2pfwB_ not modelled 7.6 19 PDB header:unknown function Chain: B: PDB Molecule:cupin 2, conserved barrel domain protein; PDBTitle: crystal structure of a rmlc-like cupin (sfri_3105) from shewanella2 frigidimarina ncimb 400 at 1.90 a resolution 42 c3emrA_ not modelled 7.3 13 PDB header:oxidoreductase Chain: A: PDB Molecule:ectd; PDBTitle: crystal structure analysis of the ectoine hydroxylase ectd from2 salibacillus salexigens 43 d2a1xa1 not modelled 6.6 27 Fold:Double-stranded beta-helix Superfamily:Clavaminate synthase-like Family:PhyH-like 44 d1y3ta1 not modelled 6.2 25 Fold:Double-stranded beta-helix Superfamily:RmlC-like cupins Family:Quercetin 2,3-dioxygenase-like 45 d1tvfa1 not modelled 6.2 27 Fold:Penicillin-binding protein associated domain Superfamily:Penicillin-binding protein associated domain Family:Pencillin binding protein 4 (PbpD), C-terminal domain 46 d2fcta1 not modelled 6.1 18 Fold:Double-stranded beta-helix Superfamily:Clavaminate synthase-like Family:PhyH-like 47 c2lazA_ not modelled 5.8 22 PDB header:signaling protein/transcription Chain: A: PDB Molecule:e3 ubiquitin-protein ligase smurf1; PDBTitle: structure of the first ww domain of human smurf1 in complex with a2 mono-phosphorylated human smad1 derived peptide 48 d1rc6a_ not modelled 5.6 22 Fold:Double-stranded beta-helix Superfamily:RmlC-like cupins Family:YlbA-like 49 d1j0sa_ not modelled 5.6 24 Fold:beta-Trefoil Superfamily:Cytokine Family:Interleukin-1 (IL-1) 50 c2nnzA_ not modelled 5.5 15 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:hypothetical protein; PDBTitle: solution structure of the hypothetical protein af2241 from2 archaeoglobus fulgidus 51 c2opwA_ not modelled 5.4 21 PDB header:oxidoreductase Chain: A: PDB Molecule:phyhd1 protein; PDBTitle: crystal structure of human phytanoyl-coa dioxygenase phyhd1 (apo) 52 c2kpsA_ not modelled 5.4 15 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:uncharacterized protein; PDBTitle: solution structure of domain iv from the ybbr family protein of2 desulfitobacterium hafniense 53 c2lb0A_ not modelled 5.3 22 PDB header:signaling protein/transcription Chain: A: PDB Molecule:e3 ubiquitin-protein ligase smurf1; PDBTitle: structure of the first ww domain of human smurf1 in complex with a di-2 phosphorylated human smad1 derived peptide

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

Transmembrane helix prediction

Transmembrane helices have been predicted in your sequence to adopt the topology shown below

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0