Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	S	I	A	M	R	L	K	V	M	S	F	L	Q	Y	F	I	W	G	S	W	L	V	T	L	G	S	Y	M	I	N	T	L	H	F	T	G	A	N	V	G	M	V	Y	S	S	K	G	I	A	A	I	I	M	P	G	I	M	G	I
Secondary structure
SS confidence
Disorder		?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		I	A	D	K	W	L	R	A	E	R	A	Y	M	L	C	H	L	V	C	A	G	V	L	F	Y	A	A	S	V	T	D	P	D	M	M	F	W	V	M	L	V	N	A	M	A	F	M	P	T	I	A	L	S	N	S	V	S	Y	S	C
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		L	A	Q	A	G	L	D	P	V	T	A	F	P	P	I	R	V	F	G	T	V	G	F	I	V	A	M	W	A	V	S	L	L	H	L	E	L	S	S	L	Q	L	Y	I	A	S	G	A	S	L	L	L	S	A	Y	A	L	T	L	P
Secondary structure
SS confidence
Disorder			?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		K	I	P	V	A	E	K	K	A	T	T	S	L	A	S	K	L	G	L	D	A	F	V	L	F	K	N	P	R	M	A	I	F	F	L	F	A	M	M	L	G	A	V	L	Q	I	T	N	V	F	G	N	P	F	L	H	D	F	A	R
Secondary structure
SS confidence
Disorder			?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?																																								?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		N	P	E	F	A	D	S	F	V	V	K	Y	P	S	I	L	L	S	V	S	Q	M	A	E	V	G	F	I	L	T	I	P	F	F	L	K	R	F	G	I	K	T	V	M	L	M	S	M	V	A	W	T	L	R	F	G	F	F	A	Y
Secondary structure
SS confidence
Disorder			?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		G	D	P	S	T	T	G	F	I	L	L	L	L	S	M	I	V	Y	G	C	A	F	D	F	F	N	I	S	G	S	V	F	V	E	Q	E	V	D	S	S	I	R	A	S	A	Q	G	L	F	M	T	M	V	N	G	V	G	A	W	V
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.
Sequence		G	S	I	L	S	G	M	A	V	D	Y	F	S	V	D	G	V	K	D	W	Q	T	I	W	L	V	F	A	G	Y	A	L	F	L	A	V	I	F	F	F	G	F	K	Y	N	H	D	P	E	K	I	K	H	R	A	V	T	H
Secondary structure
SS confidence
Disorder																		?																										?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 1pv7 chain A Region: 2 - 417 Aligned: 406 Modelled: 410 Confidence: 100.0% Identity: 12% Fold: MFS general substrate transporter Superfamily: MFS general substrate transporter Family: LacY-like proton/sugar symporter Phyre2 PDB 1pw4 chain A Region: 2 - 417 Aligned: 406 Modelled: 410 Confidence: 100.0% Identity: 12% Fold: MFS general substrate transporter Superfamily: MFS general substrate transporter Family: Glycerol-3-phosphate transporter Phyre2 PDB 2xut chain C Region: 7 - 398 Aligned: 391 Modelled: 392 Confidence: 100.0% Identity: 17% PDB header:transport protein Chain: C: PDB Molecule:proton/peptide symporter family protein; PDBTitle: crystal structure of a proton dependent oligopeptide (pot)2 family transporter. Phyre2 PDB 3o7p chain A Region: 7 - 404 Aligned: 384 Modelled: 398 Confidence: 100.0% Identity: 12% PDB header:transport protein Chain: A: PDB Molecule:l-fucose-proton symporter; PDBTitle: crystal structure of the e.coli fucose:proton symporter, fucp (n162a) Phyre2 PDB 2gfp chain A Region: 3 - 396 Aligned: 370 Modelled: 382 Confidence: 100.0% Identity: 11% PDB header:membrane protein Chain: A: PDB Molecule:multidrug resistance protein d; PDBTitle: structure of the multidrug transporter emrd from2 escherichia coli Phyre2 PDB 1fft chain B domain 2 Region: 380 - 418 Aligned: 39 Modelled: 39 Confidence: 56.6% Identity: 15% Fold: Transmembrane helix hairpin Superfamily: Cytochrome c oxidase subunit II-like, transmembrane region Family: Cytochrome c oxidase subunit II-like, transmembrane region Phyre2 PDB 2knc chain A Region: 374 - 417 Aligned: 44 Modelled: 44 Confidence: 51.6% Identity: 18% PDB header:cell adhesion Chain: A: PDB Molecule:integrin alpha-iib; PDBTitle: platelet integrin alfaiib-beta3 transmembrane-cytoplasmic2 heterocomplex Phyre2 PDB 3qnq chain D Region: 366 - 418 Aligned: 53 Modelled: 53 Confidence: 38.0% Identity: 15% PDB header:membrane protein, transport protein Chain: D: PDB Molecule:pts system, cellobiose-specific iic component; PDBTitle: crystal structure of the transporter chbc, the iic component from the2 n,n'-diacetylchitobiose-specific phosphotransferase system Phyre2 PDB 2w8a chain C Region: 9 - 209 Aligned: 201 Modelled: 201 Confidence: 25.4% Identity: 7% PDB header:membrane protein Chain: C: PDB Molecule:glycine betaine transporter betp; PDBTitle: crystal structure of the sodium-coupled glycine betaine2 symporter betp from corynebacterium glutamicum with bound3 substrate Phyre2 PDB 3dtu chain B domain 2 Region: 382 - 418 Aligned: 36 Modelled: 37 Confidence: 15.9% Identity: 14% Fold: Transmembrane helix hairpin Superfamily: Cytochrome c oxidase subunit II-like, transmembrane region Family: Cytochrome c oxidase subunit II-like, transmembrane region Phyre2 PDB 3ehb chain B domain 2 Region: 382 - 418 Aligned: 36 Modelled: 37 Confidence: 12.4% Identity: 11% Fold: Transmembrane helix hairpin Superfamily: Cytochrome c oxidase subunit II-like, transmembrane region Family: Cytochrome c oxidase subunit II-like, transmembrane region Phyre2 PDB 2rdd chain B Region: 390 - 414 Aligned: 25 Modelled: 25 Confidence: 9.2% Identity: 12% PDB header:membrane protein/transport protein Chain: B: PDB Molecule:upf0092 membrane protein yajc; PDBTitle: x-ray crystal structure of acrb in complex with a novel2 transmembrane helix. Phyre2 PDB 2g9p chain A Region: 57 - 70 Aligned: 14 Modelled: 14 Confidence: 8.7% Identity: 21% PDB header:antimicrobial protein Chain: A: PDB Molecule:antimicrobial peptide latarcin 2a; PDBTitle: nmr structure of a novel antimicrobial peptide, latarcin 2a,2 from spider (lachesana tarabaevi) venom Phyre2 PDB 2fno chain A domain 1 Region: 49 - 72 Aligned: 24 Modelled: 24 Confidence: 8.1% Identity: 17% Fold: GST C-terminal domain-like Superfamily: GST C-terminal domain-like Family: Glutathione S-transferase (GST), C-terminal domain Phyre2 PDB 2jo1 chain A Region: 378 - 417 Aligned: 40 Modelled: 40 Confidence: 7.7% Identity: 18% PDB header:hydrolase regulator Chain: A: PDB Molecule:phospholemman; PDBTitle: structure of the na,k-atpase regulatory protein fxyd1 in2 micelles Phyre2 PDB 1j4n chain A Region: 379 - 417 Aligned: 39 Modelled: 39 Confidence: 7.5% Identity: 15% Fold: Aquaporin-like Superfamily: Aquaporin-like Family: Aquaporin-like Phyre2 PDB 3b9y chain A Region: 15 - 210 Aligned: 196 Modelled: 196 Confidence: 7.1% Identity: 8% PDB header:transport protein Chain: A: PDB Molecule:ammonium transporter family rh-like protein; PDBTitle: crystal structure of the nitrosomonas europaea rh protein Phyre2 PDB 1qle chain B Region: 380 - 418 Aligned: 39 Modelled: 39 Confidence: 6.1% Identity: 8% PDB header:oxidoreductase/immune system Chain: B: PDB Molecule:cytochrome c oxidase polypeptide ii; PDBTitle: cryo-structure of the paracoccus denitrificans four-subunit2 cytochrome c oxidase in the completely oxidized state3 complexed with an antibody fv fragment Phyre2 PDB 1ar1 chain B Region: 380 - 418 Aligned: 39 Modelled: 39 Confidence: 6.1% Identity: 8% PDB header:complex (oxidoreductase/antibody) Chain: B: PDB Molecule:cytochrome c oxidase; PDBTitle: structure at 2.7 angstrom resolution of the paracoccus2 denitrificans two-subunit cytochrome c oxidase complexed3 with an antibody fv fragment Phyre2 PDB 1ymg chain A Region: 379 - 416 Aligned: 38 Modelled: 38 Confidence: 5.3% Identity: 5% PDB header:membrane protein Chain: A: PDB Molecule:lens fiber major intrinsic protein; PDBTitle: the channel architecture of aquaporin o at 2.2 angstrom resolution Phyre2 1 d1pv7a_ 2 d1pw4a_ 3 c2xutC_ 4 c3o7pA_ 5 c2gfpA_ 6 d1fftb2 7 c2kncA_ 8 c3qnqD_ 9 c2w8aC_ 10 d3dtub2 11 d3ehbb2 12 c2rddB_ 13 c2g9pA_ 14 d2fnoa1 15 c2jo1A_ 16 d1j4na_ 17 c3b9yA_ 18 c1qleB_ 19 c1ar1B_ 20 c1ymgA_ 21

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 d1pv7a_ 100.0 12 Fold:MFS general substrate transporter Superfamily:MFS general substrate transporter Family:LacY-like proton/sugar symporter 2 d1pw4a_ 100.0 12 Fold:MFS general substrate transporter Superfamily:MFS general substrate transporter Family:Glycerol-3-phosphate transporter 3 c2xutC_ 100.0 17 PDB header:transport protein Chain: C: PDB Molecule:proton/peptide symporter family protein; PDBTitle: crystal structure of a proton dependent oligopeptide (pot)2 family transporter. 4 c3o7pA_ 100.0 12 PDB header:transport protein Chain: A: PDB Molecule:l-fucose-proton symporter; PDBTitle: crystal structure of the e.coli fucose:proton symporter, fucp (n162a) 5 c2gfpA_ 100.0 11 PDB header:membrane protein Chain: A: PDB Molecule:multidrug resistance protein d; PDBTitle: structure of the multidrug transporter emrd from2 escherichia coli 6 d1fftb2 56.6 15 Fold:Transmembrane helix hairpin Superfamily:Cytochrome c oxidase subunit II-like, transmembrane region Family:Cytochrome c oxidase subunit II-like, transmembrane region 7 c2kncA_ 51.6 18 PDB header:cell adhesion Chain: A: PDB Molecule:integrin alpha-iib; PDBTitle: platelet integrin alfaiib-beta3 transmembrane-cytoplasmic2 heterocomplex 8 c3qnqD_ 38.0 15 PDB header:membrane protein, transport protein Chain: D: PDB Molecule:pts system, cellobiose-specific iic component; PDBTitle: crystal structure of the transporter chbc, the iic component from the2 n,n'-diacetylchitobiose-specific phosphotransferase system 9 c2w8aC_ 25.4 7 PDB header:membrane protein Chain: C: PDB Molecule:glycine betaine transporter betp; PDBTitle: crystal structure of the sodium-coupled glycine betaine2 symporter betp from corynebacterium glutamicum with bound3 substrate 10 d3dtub2 15.9 14 Fold:Transmembrane helix hairpin Superfamily:Cytochrome c oxidase subunit II-like, transmembrane region Family:Cytochrome c oxidase subunit II-like, transmembrane region 11 d3ehbb2 12.4 11 Fold:Transmembrane helix hairpin Superfamily:Cytochrome c oxidase subunit II-like, transmembrane region Family:Cytochrome c oxidase subunit II-like, transmembrane region 12 c2rddB_ 9.2 12 PDB header:membrane protein/transport protein Chain: B: PDB Molecule:upf0092 membrane protein yajc; PDBTitle: x-ray crystal structure of acrb in complex with a novel2 transmembrane helix. 13 c2g9pA_ 8.7 21 PDB header:antimicrobial protein Chain: A: PDB Molecule:antimicrobial peptide latarcin 2a; PDBTitle: nmr structure of a novel antimicrobial peptide, latarcin 2a,2 from spider (lachesana tarabaevi) venom 14 d2fnoa1 8.1 17 Fold:GST C-terminal domain-like Superfamily:GST C-terminal domain-like Family:Glutathione S-transferase (GST), C-terminal domain 15 c2jo1A_ 7.7 18 PDB header:hydrolase regulator Chain: A: PDB Molecule:phospholemman; PDBTitle: structure of the na,k-atpase regulatory protein fxyd1 in2 micelles 16 d1j4na_ 7.5 15 Fold:Aquaporin-like Superfamily:Aquaporin-like Family:Aquaporin-like 17 c3b9yA_ 7.1 8 PDB header:transport protein Chain: A: PDB Molecule:ammonium transporter family rh-like protein; PDBTitle: crystal structure of the nitrosomonas europaea rh protein 18 c1qleB_ 6.1 8 PDB header:oxidoreductase/immune system Chain: B: PDB Molecule:cytochrome c oxidase polypeptide ii; PDBTitle: cryo-structure of the paracoccus denitrificans four-subunit2 cytochrome c oxidase in the completely oxidized state3 complexed with an antibody fv fragment 19 c1ar1B_ 6.1 8 PDB header:complex (oxidoreductase/antibody) Chain: B: PDB Molecule:cytochrome c oxidase; PDBTitle: structure at 2.7 angstrom resolution of the paracoccus2 denitrificans two-subunit cytochrome c oxidase complexed3 with an antibody fv fragment 20 c1ymgA_ 5.3 5 PDB header:membrane protein Chain: A: PDB Molecule:lens fiber major intrinsic protein; PDBTitle: the channel architecture of aquaporin o at 2.2 angstrom resolution 21 d1ymga1 not modelled 5.3 5 Fold:Aquaporin-like Superfamily:Aquaporin-like Family:Aquaporin-like

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

Transmembrane helix prediction

Transmembrane helices have been predicted in your sequence to adopt the topology shown below

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0