Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	A	Q	V	I	N	E	M	D	V	P	S	H	S	F	V	F	H	G	T	G	E	R	Y	F	L	I	C	V	V	N	V	L	L	T	I	I	T	L	G	I	Y	L	P	W	A	L	M	K	C	K	R	Y	L	Y	A	N	M	E	V
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		N	G	Q	R	F	S	Y	G	I	T	G	G	N	V	F	V	S	C	L	F	F	V	F	F	Y	F	A	I	L	M	T	V	S	A	D	M	P	L	V	G	C	V	L	T	L	L	L	L	V	L	L	I	F	M	A	A	K	G	L	R
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		H	Q	A	L	M	T	S	L	N	G	V	R	F	S	F	N	C	S	M	K	G	F	W	W	V	T	F	F	L	P	I	L	M	A	I	G	M	G	T	V	F	F	I	S	T	K	M	L	P	A	N	S	S	S	S	V	I	I	S	M
Secondary structure
SS confidence
Disorder																																															?	?	?	?	?	?	?	?				?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		V	L	M	A	I	V	G	I	V	S	I	G	I	F	N	G	T	L	Y	S	L	V	M	S	F	L	W	S	N	T	S	F	G	I	H	R	F	K	V	K	L	D	T	T	Y	C	I	K	Y	A	I	L	A	F	L	A	L	L	P	F
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		L	A	V	A	G	Y	I	I	F	D	Q	I	L	N	A	Y	D	S	S	V	Y	A	N	D	D	I	E	N	L	Q	Q	F	M	E	M	Q	R	K	M	I	I	A	Q	L	I	Y	Y	F	G	I	A	V	S	T	S	Y	L	T	V	S
Secondary structure
SS confidence
Disorder																	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		L	R	N	H	F	M	S	N	L	S	L	N	D	G	R	I	R	F	R	L	T	L	T	Y	H	G	M	L	Y	R	M	C	A	L	V	V	I	S	G	I	T	G	G	L	A	Y	P	L	L	K	I	W	M	I	D	W	Q	A	K	N
Secondary structure
SS confidence
Disorder															?																																													?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.
Sequence		T	Y	L	L	G	D	L	D	D	L	P	L	I	N	K	E	E	Q	P	D	K	G	F	L	A	S	I	S	R	G	V	M	P	S	L	P	F	L
Secondary structure
SS confidence
Disorder		?	?			?								?	?	?	?	?	?	?	?	?	?										?	?	?	?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 2y69 chain Y Region: 331 - 353 Aligned: 23 Modelled: 23 Confidence: 31.2% Identity: 13% PDB header:electron transport Chain: Y: PDB Molecule:cytochrome c oxidase subunit 7c; PDBTitle: bovine heart cytochrome c oxidase re-refined with molecular2 oxygen Phyre2 PDB 3pcs chain B Region: 33 - 44 Aligned: 12 Modelled: 12 Confidence: 23.4% Identity: 25% PDB header:protein transport/transferase Chain: B: PDB Molecule:espg; PDBTitle: structure of espg-pak2 autoinhibitory ialpha3 helix complex Phyre2 PDB 2k21 chain A Region: 3 - 51 Aligned: 49 Modelled: 49 Confidence: 20.2% Identity: 20% PDB header:membrane protein Chain: A: PDB Molecule:potassium voltage-gated channel subfamily e PDBTitle: nmr structure of human kcne1 in lmpg micelles at ph 6.0 and2 40 degree c Phyre2 PDB 1rzh chain H domain 2 Region: 36 - 45 Aligned: 10 Modelled: 10 Confidence: 19.3% Identity: 40% Fold: Single transmembrane helix Superfamily: Photosystem II reaction centre subunit H, transmembrane region Family: Photosystem II reaction centre subunit H, transmembrane region Phyre2 PDB 1l9b chain H domain 2 Region: 36 - 45 Aligned: 10 Modelled: 10 Confidence: 18.4% Identity: 40% Fold: Single transmembrane helix Superfamily: Photosystem II reaction centre subunit H, transmembrane region Family: Photosystem II reaction centre subunit H, transmembrane region Phyre2 PDB 2rcr chain H domain 2 Region: 36 - 45 Aligned: 10 Modelled: 10 Confidence: 16.3% Identity: 40% Fold: Single transmembrane helix Superfamily: Photosystem II reaction centre subunit H, transmembrane region Family: Photosystem II reaction centre subunit H, transmembrane region Phyre2 PDB 3bux chain B domain 2 Region: 19 - 36 Aligned: 18 Modelled: 18 Confidence: 16.3% Identity: 17% Fold: N-cbl like Superfamily: N-terminal domain of cbl (N-cbl) Family: N-terminal domain of cbl (N-cbl) Phyre2 PDB 4a18 chain P Region: 47 - 55 Aligned: 9 Modelled: 9 Confidence: 16.0% Identity: 44% PDB header:ribosome Chain: P: PDB Molecule:rpl38; PDBTitle: t.thermophila 60s ribosomal subunit in complex with initiation2 factor 6. this file contains 26s rrna and proteins of molecule 1 Phyre2 PDB 3b4s chain A Region: 352 - 387 Aligned: 36 Modelled: 36 Confidence: 15.5% Identity: 17% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:protein luxt; PDBTitle: crystal structure of a luxt domain from vibrio2 parahaemolyticus rimd 2210633 Phyre2 PDB 3iz5 chain N Region: 47 - 55 Aligned: 9 Modelled: 9 Confidence: 13.3% Identity: 44% PDB header:ribosome Chain: N: PDB Molecule:60s ribosomal protein l14 (l14e); PDBTitle: localization of the large subunit ribosomal proteins into a 5.5 a2 cryo-em map of triticum aestivum translating 80s ribosome Phyre2 PDB 3izc chain N Region: 47 - 55 Aligned: 9 Modelled: 9 Confidence: 12.4% Identity: 22% PDB header:ribosome Chain: N: PDB Molecule:60s ribosomal protein rpl14 (l14e); PDBTitle: localization of the large subunit ribosomal proteins into a 6.1 a2 cryo-em map of saccharomyces cerevisiae translating 80s ribosome Phyre2 PDB 1vcc chain A Region: 50 - 60 Aligned: 11 Modelled: 11 Confidence: 12.2% Identity: 36% Fold: DNA topoisomerase I domain Superfamily: DNA topoisomerase I domain Family: Vaccinia DNA topoisomerase I, 9 kDa N-terminal fragment Phyre2 PDB 1q90 chain L Region: 322 - 351 Aligned: 27 Modelled: 30 Confidence: 10.4% Identity: 30% PDB header:photosynthesis Chain: L: PDB Molecule:cytochrome b6f complex subunit petl; PDBTitle: structure of the cytochrome b6f (plastohydroquinone : plastocyanin2 oxidoreductase) from chlamydomonas reinhardtii Phyre2 PDB 1q90 chain L Region: 322 - 351 Aligned: 27 Modelled: 30 Confidence: 10.4% Identity: 30% Fold: Single transmembrane helix Superfamily: PetL subunit of the cytochrome b6f complex Family: PetL subunit of the cytochrome b6f complex Phyre2 PDB 1v54 chain L Region: 331 - 354 Aligned: 24 Modelled: 24 Confidence: 9.5% Identity: 13% Fold: Single transmembrane helix Superfamily: Mitochondrial cytochrome c oxidase subunit VIIc (aka VIIIa) Family: Mitochondrial cytochrome c oxidase subunit VIIc (aka VIIIa) Phyre2 PDB 2gw4 chain C Region: 59 - 71 Aligned: 13 Modelled: 13 Confidence: 8.7% Identity: 38% PDB header:luminescent protein Chain: C: PDB Molecule:kaede; PDBTitle: crystal structure of stony coral fluorescent protein kaede, red form Phyre2 PDB 3lf4 chain A Region: 59 - 71 Aligned: 13 Modelled: 13 Confidence: 8.5% Identity: 38% PDB header:fluorescent protein Chain: A: PDB Molecule:fluorescent timer precursor blue102; PDBTitle: crystal structure of fluorescent timer precursor blue102 Phyre2 PDB 1r0v chain A domain 3 Region: 51 - 57 Aligned: 7 Modelled: 7 Confidence: 7.1% Identity: 43% Fold: MutS N-terminal domain-like Superfamily: tRNA-intron endonuclease N-terminal domain-like Family: tRNA-intron endonuclease N-terminal domain-like Phyre2 PDB 2a56 chain A Region: 59 - 71 Aligned: 13 Modelled: 13 Confidence: 7.0% Identity: 38% PDB header:luminescent protein Chain: A: PDB Molecule:gfp-like non-fluorescent chromoprotein fp595 chain 1; PDBTitle: fluorescent protein asfp595, a143s, on-state, 5min irradiation Phyre2 PDB 3hzp chain A Region: 62 - 71 Aligned: 10 Modelled: 10 Confidence: 6.8% Identity: 40% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:ntf2-like protein of unknown function; PDBTitle: crystal structure of ntf2-like protein of unknown function mn2a_05052 from prochlorococcus marinus (yp_291699.1) from prochlorococcus sp.3 natl2a at 1.40 a resolution Phyre2 1 c2y69Y_ 2 c3pcsB_ 3 c2k21A_ 4 d1rzhh2 5 d1l9bh2 6 d2rcrh2 7 d3buxb2 8 c4a18P_ 9 c3b4sA_ 10 c3iz5n_ 11 c3izcn_ 12 d1vcca_ 13 c1q90L_ 14 d1q90l_ 15 d1v54l_ 16 c2gw4C_ 17 c3lf4A_ 18 d1r0va3 19 c2a56A_ 20 c3hzpA_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 c2y69Y_ 31.2 13 PDB header:electron transport Chain: Y: PDB Molecule:cytochrome c oxidase subunit 7c; PDBTitle: bovine heart cytochrome c oxidase re-refined with molecular2 oxygen 2 c3pcsB_ 23.4 25 PDB header:protein transport/transferase Chain: B: PDB Molecule:espg; PDBTitle: structure of espg-pak2 autoinhibitory ialpha3 helix complex 3 c2k21A_ 20.2 20 PDB header:membrane protein Chain: A: PDB Molecule:potassium voltage-gated channel subfamily e PDBTitle: nmr structure of human kcne1 in lmpg micelles at ph 6.0 and2 40 degree c 4 d1rzhh2 19.3 40 Fold:Single transmembrane helix Superfamily:Photosystem II reaction centre subunit H, transmembrane region Family:Photosystem II reaction centre subunit H, transmembrane region 5 d1l9bh2 18.4 40 Fold:Single transmembrane helix Superfamily:Photosystem II reaction centre subunit H, transmembrane region Family:Photosystem II reaction centre subunit H, transmembrane region 6 d2rcrh2 16.3 40 Fold:Single transmembrane helix Superfamily:Photosystem II reaction centre subunit H, transmembrane region Family:Photosystem II reaction centre subunit H, transmembrane region 7 d3buxb2 16.3 17 Fold:N-cbl like Superfamily:N-terminal domain of cbl (N-cbl) Family:N-terminal domain of cbl (N-cbl) 8 c4a18P_ 16.0 44 PDB header:ribosome Chain: P: PDB Molecule:rpl38; PDBTitle: t.thermophila 60s ribosomal subunit in complex with initiation2 factor 6. this file contains 26s rrna and proteins of molecule 1 9 c3b4sA_ 15.5 17 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:protein luxt; PDBTitle: crystal structure of a luxt domain from vibrio2 parahaemolyticus rimd 2210633 10 c3iz5n_ 13.3 44 PDB header:ribosome Chain: N: PDB Molecule:60s ribosomal protein l14 (l14e); PDBTitle: localization of the large subunit ribosomal proteins into a 5.5 a2 cryo-em map of triticum aestivum translating 80s ribosome 11 c3izcn_ 12.4 22 PDB header:ribosome Chain: N: PDB Molecule:60s ribosomal protein rpl14 (l14e); PDBTitle: localization of the large subunit ribosomal proteins into a 6.1 a2 cryo-em map of saccharomyces cerevisiae translating 80s ribosome 12 d1vcca_ 12.2 36 Fold:DNA topoisomerase I domain Superfamily:DNA topoisomerase I domain Family:Vaccinia DNA topoisomerase I, 9 kDa N-terminal fragment 13 c1q90L_ 10.4 30 PDB header:photosynthesis Chain: L: PDB Molecule:cytochrome b6f complex subunit petl; PDBTitle: structure of the cytochrome b6f (plastohydroquinone : plastocyanin2 oxidoreductase) from chlamydomonas reinhardtii 14 d1q90l_ 10.4 30 Fold:Single transmembrane helix Superfamily:PetL subunit of the cytochrome b6f complex Family:PetL subunit of the cytochrome b6f complex 15 d1v54l_ 9.5 13 Fold:Single transmembrane helix Superfamily:Mitochondrial cytochrome c oxidase subunit VIIc (aka VIIIa) Family:Mitochondrial cytochrome c oxidase subunit VIIc (aka VIIIa) 16 c2gw4C_ 8.7 38 PDB header:luminescent protein Chain: C: PDB Molecule:kaede; PDBTitle: crystal structure of stony coral fluorescent protein kaede, red form 17 c3lf4A_ 8.5 38 PDB header:fluorescent protein Chain: A: PDB Molecule:fluorescent timer precursor blue102; PDBTitle: crystal structure of fluorescent timer precursor blue102 18 d1r0va3 7.1 43 Fold:MutS N-terminal domain-like Superfamily:tRNA-intron endonuclease N-terminal domain-like Family:tRNA-intron endonuclease N-terminal domain-like 19 c2a56A_ 7.0 38 PDB header:luminescent protein Chain: A: PDB Molecule:gfp-like non-fluorescent chromoprotein fp595 chain 1; PDBTitle: fluorescent protein asfp595, a143s, on-state, 5min irradiation 20 c3hzpA_ 6.8 40 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:ntf2-like protein of unknown function; PDBTitle: crystal structure of ntf2-like protein of unknown function mn2a_05052 from prochlorococcus marinus (yp_291699.1) from prochlorococcus sp.3 natl2a at 1.40 a resolution 21 c1xrxD_ not modelled 6.7 21 PDB header:replication inhibitor Chain: D: PDB Molecule:seqa protein; PDBTitle: crystal structure of a dna-binding protein 22 d1xrxa1 not modelled 6.7 21 Fold:Ribbon-helix-helix Superfamily:Ribbon-helix-helix Family:SeqA N-terminal domain-like 23 c3op0B_ not modelled 6.4 22 PDB header:signaling protein/signaling protein regu Chain: B: PDB Molecule:signal transduction protein cbl-c; PDBTitle: crystal structure of cbl-c (cbl-3) tkb domain in complex with egfr2 py1069 peptide 24 c2cblA_ not modelled 6.3 17 PDB header:complex (proto-oncogene/peptide) Chain: A: PDB Molecule:proto-oncogene cbl; PDBTitle: n-terminal domain of cbl in complex with its binding site2 on zap-70 25 c3bunB_ not modelled 6.3 17 PDB header:ligase/signaling protein Chain: B: PDB Molecule:e3 ubiquitin-protein ligase cbl; PDBTitle: crystal structure of c-cbl-tkb domain complexed with its2 binding motif in sprouty4 26 c2yvxD_ not modelled 5.9 7 PDB header:transport protein Chain: D: PDB Molecule:mg2+ transporter mgte; PDBTitle: crystal structure of magnesium transporter mgte 27 d1ckva_ not modelled 5.8 10 Fold:Monooxygenase (hydroxylase) regulatory protein Superfamily:Monooxygenase (hydroxylase) regulatory protein Family:Monooxygenase (hydroxylase) regulatory protein 28 c2npiB_ not modelled 5.6 15 PDB header:transcription Chain: B: PDB Molecule:protein clp1; PDBTitle: clp1-atp-pcf11 complex 29 c3zrgB_ not modelled 5.6 18 PDB header:protein binding Chain: B: PDB Molecule:pexrd2 family secreted rxlr effector peptide, putative; PDBTitle: crystal structure of rxlr effector pexrd2 from phytophthora infestans 30 c3l32B_ not modelled 5.6 44 PDB header:viral protein Chain: B: PDB Molecule:phosphoprotein; PDBTitle: structure of the dimerisation domain of the rabies virus2 phosphoprotein 31 d1dvoa_ not modelled 5.6 22 Fold:FinO-like Superfamily:FinO-like Family:FinO-like 32 c1fbvA_ not modelled 5.6 17 PDB header:ligase Chain: A: PDB Molecule:signal transduction protein cbl; PDBTitle: structure of a cbl-ubch7 complex: ring domain function in2 ubiquitin-protein ligases 33 c2jo1A_ not modelled 5.4 30 PDB header:hydrolase regulator Chain: A: PDB Molecule:phospholemman; PDBTitle: structure of the na,k-atpase regulatory protein fxyd1 in2 micelles 34 c3t2dA_ not modelled 5.4 6 PDB header:lyase, hydrolase Chain: A: PDB Molecule:fructose-1,6-bisphosphate aldolase/phosphatase; PDBTitle: fructose-1,6-bisphosphate aldolase/phosphatase from thermoproteus2 neutrophilus, fbp-bound form 35 d2csba5 not modelled 5.4 44 Fold:Topoisomerase V catalytic domain-like Superfamily:Topoisomerase V catalytic domain-like Family:Topoisomerase V catalytic domain-like 36 c3sjaG_ not modelled 5.3 30 PDB header:hydrolase/transport protein Chain: G: PDB Molecule:golgi to er traffic protein 1; PDBTitle: crystal structure of s. cerevisiae get3 in the open state in complex2 with get1 cytosolic domain 37 c2jp3A_ not modelled 5.1 30 PDB header:transcription Chain: A: PDB Molecule:fxyd domain-containing ion transport regulator 4; PDBTitle: solution structure of the human fxyd4 (chif) protein in sds2 micelles

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0