Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	P	L	P	D	F	R	L	I	R	L	L	P	L	A	A	L	V	L	T	A	C	S	V	T	T	P	K	G	P	G	K	S	P	D	S	P	Q	W	R	Q	H	Q	Q	D	V	R	N	L	N	Q	Y	Q	T	R	G	A	F	A	Y
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?																?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?										?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		I	S	D	Q	Q	K	V	Y	A	R	F	F	W	Q	Q	T	G	Q	D	R	Y	R	L	L	L	T	N	P	L	G	S	T	E	L	E	L	N	A	Q	P	G	N	V	Q	L	V	D	N	K	G	Q	R	Y	T	A	D	D	A	E	E
Secondary structure
SS confidence
Disorder			?	?	?	?	?										?																																		?	?			?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		M	I	G	K	L	T	G	M	P	I	P	L	N	S	L	R	Q	W	I	L	G	L	P	G	D	A	T	D	Y	K	L	D	D	Q	Y	R	L	S	E	I	T	Y	S	Q	N	G	K	N	W	K	V	V	Y	G	G	Y	D	T	K	T
Secondary structure
SS confidence
Disorder																																						?						?	?	?	?													?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.
Sequence		Q	P	A	M	P	A	N	M	E	L	T	D	G	G	Q	R	I	K	L	K	M	D	N	W	I	V	K
Secondary structure
SS confidence
Disorder		?																					?	?	?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 1iwm chain A Region: 21 - 207 Aligned: 177 Modelled: 187 Confidence: 100.0% Identity: 97% Fold: LolA-like prokaryotic lipoproteins and lipoprotein localization factors Superfamily: Prokaryotic lipoproteins and lipoprotein localization factors Family: Outer membrane lipoprotein receptor LolB Phyre2 PDB 3buu chain B Region: 32 - 207 Aligned: 169 Modelled: 176 Confidence: 76.7% Identity: 12% PDB header:structural genomics, unknown function Chain: B: PDB Molecule:uncharacterized lola superfamily protein ne2245; PDBTitle: crystal structure of lola superfamily protein ne2245 from2 nitrosomonas europaea Phyre2 PDB 1ekg chain A Region: 69 - 137 Aligned: 65 Modelled: 69 Confidence: 39.7% Identity: 15% Fold: N domain of copper amine oxidase-like Superfamily: Frataxin/Nqo15-like Family: Frataxin-like Phyre2 PDB 2w7q chain B Region: 36 - 206 Aligned: 155 Modelled: 166 Confidence: 35.9% Identity: 12% PDB header:protein transport Chain: B: PDB Molecule:outer-membrane lipoprotein carrier protein; PDBTitle: structure of pseudomonas aeruginosa lola Phyre2 PDB 2cc3 chain A domain 1 Region: 65 - 104 Aligned: 40 Modelled: 40 Confidence: 25.1% Identity: 23% Fold: Cystatin-like Superfamily: NTF2-like Family: VirB8-like Phyre2 PDB 1tp6 chain A Region: 127 - 181 Aligned: 52 Modelled: 55 Confidence: 24.8% Identity: 10% Fold: Cystatin-like Superfamily: NTF2-like Family: PA1314-like Phyre2 PDB 1iwl chain A Region: 40 - 114 Aligned: 69 Modelled: 75 Confidence: 24.2% Identity: 10% Fold: LolA-like prokaryotic lipoproteins and lipoprotein localization factors Superfamily: Prokaryotic lipoproteins and lipoprotein localization factors Family: Outer-membrane lipoproteins carrier protein LolA Phyre2 PDB 1vpk chain A domain 2 Region: 92 - 176 Aligned: 81 Modelled: 85 Confidence: 16.8% Identity: 15% Fold: DNA clamp Superfamily: DNA clamp Family: DNA polymerase III, beta subunit Phyre2 PDB 1ttz chain A Region: 126 - 143 Aligned: 18 Modelled: 18 Confidence: 15.8% Identity: 28% Fold: Thioredoxin fold Superfamily: Thioredoxin-like Family: Thioltransferase Phyre2 PDB 2fgy chain A Region: 118 - 142 Aligned: 25 Modelled: 25 Confidence: 14.3% Identity: 28% PDB header:lyase Chain: A: PDB Molecule:carboxysome shell polypeptide; PDBTitle: beta carbonic anhydrase from the carboxysomal shell of2 halothiobacillus neapolitanus (csosca) Phyre2 PDB 3ls1 chain A Region: 19 - 51 Aligned: 32 Modelled: 33 Confidence: 13.6% Identity: 19% PDB header:photosynthesis Chain: A: PDB Molecule:sll1638 protein; PDBTitle: crystal structure of cyanobacterial psbq from synechocystis2 sp. pcc 6803 complexed with zn2+ Phyre2 PDB 3gf8 chain A Region: 188 - 207 Aligned: 20 Modelled: 20 Confidence: 13.6% Identity: 10% PDB header:carbohydrate binding protein Chain: A: PDB Molecule:putative polysaccharide binding proteins (duf1812); PDBTitle: crystal structure of putative polysaccharide binding proteins2 (duf1812) (np_809975.1) from bacteroides thetaiotaomicron vpi-5482 at3 2.20 a resolution Phyre2 PDB 3d0j chain A Region: 149 - 202 Aligned: 50 Modelled: 54 Confidence: 11.9% Identity: 14% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:uncharacterized protein ca_c3497; PDBTitle: crystal structure of conserved protein of unknown function ca_c34972 from clostridium acetobutylicum atcc 824 Phyre2 PDB 1jb7 chain B Region: 199 - 207 Aligned: 9 Modelled: 9 Confidence: 11.9% Identity: 22% Fold: OB-fold Superfamily: Nucleic acid-binding proteins Family: Single strand DNA-binding domain, SSB Phyre2 PDB 2i0q chain B Region: 199 - 207 Aligned: 9 Modelled: 9 Confidence: 11.9% Identity: 22% PDB header:structural protein/dna Chain: B: PDB Molecule:telomere-binding protein beta subunit; PDBTitle: crystal structure of a telomere single-strand dna-protein2 complex from o. nova with full-length alpha and beta3 telomere proteins Phyre2 PDB 3f10 chain A Region: 70 - 143 Aligned: 65 Modelled: 74 Confidence: 11.9% Identity: 9% PDB header:hydrolase, lyase Chain: A: PDB Molecule:8-oxoguanine-dna-glycosylase; PDBTitle: crystal structure of clostridium acetobutylicum 8-2 oxoguanine dna glycosylase in complex with 8-oxoguanosine Phyre2 PDB 1otc chain B Region: 199 - 207 Aligned: 9 Modelled: 9 Confidence: 10.3% Identity: 22% PDB header:protein/dna Chain: B: PDB Molecule:protein (telomere-binding protein beta subunit); PDBTitle: the o. nova telomere end binding protein complexed with2 single strand dna Phyre2 PDB 2yh5 chain A Region: 77 - 125 Aligned: 40 Modelled: 49 Confidence: 9.6% Identity: 25% PDB header:lipid binding protein Chain: A: PDB Molecule:dapx protein; PDBTitle: structure of the c-terminal domain of bamc Phyre2 PDB 2noh chain A domain 2 Region: 70 - 138 Aligned: 65 Modelled: 69 Confidence: 8.9% Identity: 18% Fold: TBP-like Superfamily: TATA-box binding protein-like Family: DNA repair glycosylase, N-terminal domain Phyre2 PDB 2hiy chain A domain 1 Region: 89 - 148 Aligned: 60 Modelled: 60 Confidence: 8.3% Identity: 15% Fold: SP0830-like Superfamily: SP0830-like Family: SP0830-like Phyre2 1 d1iwma_ 2 c3buuB_ 3 d1ekga_ 4 c2w7qB_ 5 d2cc3a1 6 d1tp6a_ 7 d1iwla_ 8 d1vpka2 9 d1ttza_ 10 c2fgyA_ 11 c3ls1A_ 12 c3gf8A_ 13 c3d0jA_ 14 d1jb7b_ 15 c2i0qB_ 16 c3f10A_ 17 c1otcB_ 18 c2yh5A_ 19 d2noha2 20 d2hiya1 21 22 23 24 25 26 27 28 29

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 d1iwma_ 100.0 97 Fold:LolA-like prokaryotic lipoproteins and lipoprotein localization factors Superfamily:Prokaryotic lipoproteins and lipoprotein localization factors Family:Outer membrane lipoprotein receptor LolB 2 c3buuB_ 76.7 12 PDB header:structural genomics, unknown function Chain: B: PDB Molecule:uncharacterized lola superfamily protein ne2245; PDBTitle: crystal structure of lola superfamily protein ne2245 from2 nitrosomonas europaea 3 d1ekga_ 39.7 15 Fold:N domain of copper amine oxidase-like Superfamily:Frataxin/Nqo15-like Family:Frataxin-like 4 c2w7qB_ 35.9 12 PDB header:protein transport Chain: B: PDB Molecule:outer-membrane lipoprotein carrier protein; PDBTitle: structure of pseudomonas aeruginosa lola 5 d2cc3a1 25.1 23 Fold:Cystatin-like Superfamily:NTF2-like Family:VirB8-like 6 d1tp6a_ 24.8 10 Fold:Cystatin-like Superfamily:NTF2-like Family:PA1314-like 7 d1iwla_ 24.2 10 Fold:LolA-like prokaryotic lipoproteins and lipoprotein localization factors Superfamily:Prokaryotic lipoproteins and lipoprotein localization factors Family:Outer-membrane lipoproteins carrier protein LolA 8 d1vpka2 16.8 15 Fold:DNA clamp Superfamily:DNA clamp Family:DNA polymerase III, beta subunit 9 d1ttza_ 15.8 28 Fold:Thioredoxin fold Superfamily:Thioredoxin-like Family:Thioltransferase 10 c2fgyA_ 14.3 28 PDB header:lyase Chain: A: PDB Molecule:carboxysome shell polypeptide; PDBTitle: beta carbonic anhydrase from the carboxysomal shell of2 halothiobacillus neapolitanus (csosca) 11 c3ls1A_ 13.6 19 PDB header:photosynthesis Chain: A: PDB Molecule:sll1638 protein; PDBTitle: crystal structure of cyanobacterial psbq from synechocystis2 sp. pcc 6803 complexed with zn2+ 12 c3gf8A_ 13.6 10 PDB header:carbohydrate binding protein Chain: A: PDB Molecule:putative polysaccharide binding proteins (duf1812); PDBTitle: crystal structure of putative polysaccharide binding proteins2 (duf1812) (np_809975.1) from bacteroides thetaiotaomicron vpi-5482 at3 2.20 a resolution 13 c3d0jA_ 11.9 14 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:uncharacterized protein ca_c3497; PDBTitle: crystal structure of conserved protein of unknown function ca_c34972 from clostridium acetobutylicum atcc 824 14 d1jb7b_ 11.9 22 Fold:OB-fold Superfamily:Nucleic acid-binding proteins Family:Single strand DNA-binding domain, SSB 15 c2i0qB_ 11.9 22 PDB header:structural protein/dna Chain: B: PDB Molecule:telomere-binding protein beta subunit; PDBTitle: crystal structure of a telomere single-strand dna-protein2 complex from o. nova with full-length alpha and beta3 telomere proteins 16 c3f10A_ 11.9 9 PDB header:hydrolase, lyase Chain: A: PDB Molecule:8-oxoguanine-dna-glycosylase; PDBTitle: crystal structure of clostridium acetobutylicum 8-2 oxoguanine dna glycosylase in complex with 8-oxoguanosine 17 c1otcB_ 10.3 22 PDB header:protein/dna Chain: B: PDB Molecule:protein (telomere-binding protein beta subunit); PDBTitle: the o. nova telomere end binding protein complexed with2 single strand dna 18 c2yh5A_ 9.6 25 PDB header:lipid binding protein Chain: A: PDB Molecule:dapx protein; PDBTitle: structure of the c-terminal domain of bamc 19 d2noha2 8.9 18 Fold:TBP-like Superfamily:TATA-box binding protein-like Family:DNA repair glycosylase, N-terminal domain 20 d2hiya1 8.3 15 Fold:SP0830-like Superfamily:SP0830-like Family:SP0830-like 21 d1p8ja1 not modelled 8.2 24 Fold:Galactose-binding domain-like Superfamily:Galactose-binding domain-like Family:Proprotein convertase P-domain 22 d1zy9a1 not modelled 7.8 18 Fold:Supersandwich Superfamily:Galactose mutarotase-like Family:YicI N-terminal domain-like 23 d1ew4a_ not modelled 7.0 9 Fold:N domain of copper amine oxidase-like Superfamily:Frataxin/Nqo15-like Family:Frataxin-like 24 c3bmbB_ not modelled 6.7 11 PDB header:rna binding protein Chain: B: PDB Molecule:regulator of nucleoside diphosphate kinase; PDBTitle: crystal structure of a new rna polymerase interacting2 protein 25 c2pn0D_ not modelled 6.5 22 PDB header:transcription Chain: D: PDB Molecule:prokaryotic transcription elongation factor PDBTitle: prokaryotic transcription elongation factor grea/greb from2 nitrosomonas europaea 26 d2id4a1 not modelled 6.4 14 Fold:Galactose-binding domain-like Superfamily:Galactose-binding domain-like Family:Proprotein convertase P-domain 27 c3ot2B_ not modelled 6.2 10 PDB header:structural genomics, unknown function Chain: B: PDB Molecule:uncharacterized protein; PDBTitle: crystal structure of a putative nuclease belonging to duf8202 (ava_3926) from anabaena variabilis atcc 29413 at 1.96 a resolution 28 c3ot2A_ not modelled 6.2 10 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:uncharacterized protein; PDBTitle: crystal structure of a putative nuclease belonging to duf8202 (ava_3926) from anabaena variabilis atcc 29413 at 1.96 a resolution 29 c2xetB_ not modelled 6.2 21 PDB header:transport protein Chain: B: PDB Molecule:f1 capsule-anchoring protein; PDBTitle: conserved hydrophobic clusters on the surface of the caf1a usher2 c-terminal domain are important for f1 antigen assembly

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

Transmembrane helix prediction

Transmembrane helices have been predicted in your sequence to adopt the topology shown below

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0