Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	D	T	S	L	A	E	E	V	Q	Q	T	M	A	T	L	A	P	N	R	F	F	F	M	S	P	Y	R	S	F	T	T	S	G	C	F	A	R	F	D	E	P	A	V	N	G	D	S	P	D	S	P	F	Q	Q	K	L	A	A	L
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?					?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		F	A	D	A	K	A	Q	G	I	K	N	P	V	M	V	G	A	I	P	F	D	P	R	Q	P	S	S	L	Y	I	P	E	S	W	Q	S	F	S	R	Q	E	K	Q	A	S	A	R	R	F	T	R	S	Q	S	L	N	V	V	E	R
Secondary structure
SS confidence
Disorder																																												?				?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		Q	A	I	P	E	Q	T	T	F	E	Q	M	V	A	R	A	A	A	L	T	A	T	P	Q	V	D	K	V	V	L	S	R	L	I	D	I	T	T	D	A	A	I	D	S	G	V	L	L	E	R	L	I	A	Q	N	P	V	S	Y	N
Secondary structure
SS confidence
Disorder		?	?			?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		F	H	V	P	L	A	D	G	G	V	L	L	G	A	S	P	E	L	L	L	R	K	D	G	E	R	F	S	S	I	P	L	A	G	S	A	R	R	Q	P	D	E	V	L	D	R	E	A	G	N	R	L	L	A	S	E	K	D	R	H
Secondary structure
SS confidence
Disorder																																									?	?												?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		E	H	E	L	V	T	Q	A	M	K	E	V	L	R	E	R	S	S	E	L	H	V	P	S	S	P	Q	L	I	T	T	P	T	L	W	H	L	A	T	P	F	E	G	K	A	N	S	Q	E	N	A	L	T	L	A	C	L	L	H	P
Secondary structure
SS confidence
Disorder																									?		?				?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		T	P	A	L	S	G	F	P	H	Q	A	A	T	Q	V	I	A	E	L	E	P	F	D	R	E	L	F	G	G	I	V	G	W	C	D	S	E	G	N	G	E	W	V	V	T	I	R	C	A	K	L	R	E	N	Q	V	R	L	F	A
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.
Sequence		G	A	G	I	V	P	A	S	S	P	L	G	E	W	R	E	T	G	V	K	L	S	T	M	L	N	V	F	G	L	H
Secondary structure
SS confidence
Disorder																													?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 3hwo chain B Region: 14 - 391 Aligned: 378 Modelled: 378 Confidence: 100.0% Identity: 100% PDB header:isomerase Chain: B: PDB Molecule:isochorismate synthase entc; PDBTitle: crystal structure of escherichia coli enterobactin-specific2 isochorismate synthase entc in complex with isochorismate Phyre2 PDB 3os6 chain A Region: 7 - 391 Aligned: 368 Modelled: 367 Confidence: 100.0% Identity: 38% PDB header:isomerase Chain: A: PDB Molecule:isochorismate synthase dhbc; PDBTitle: crystal structure of putative 2,3-dihydroxybenzoate-specific2 isochorismate synthase, dhbc from bacillus anthracis. Phyre2 PDB 3bzn chain A domain 1 Region: 19 - 388 Aligned: 354 Modelled: 358 Confidence: 100.0% Identity: 27% Fold: ADC synthase Superfamily: ADC synthase Family: ADC synthase Phyre2 PDB 1qdl chain A Region: 6 - 390 Aligned: 374 Modelled: 385 Confidence: 100.0% Identity: 25% Fold: ADC synthase Superfamily: ADC synthase Family: ADC synthase Phyre2 PDB 2fn0 chain A domain 1 Region: 1 - 390 Aligned: 378 Modelled: 390 Confidence: 100.0% Identity: 22% Fold: ADC synthase Superfamily: ADC synthase Family: ADC synthase Phyre2 PDB 3gse chain A Region: 19 - 384 Aligned: 348 Modelled: 366 Confidence: 100.0% Identity: 24% PDB header:isomerase Chain: A: PDB Molecule:menaquinone-specific isochorismate synthase; PDBTitle: crystal structure of menaquinone-specific isochorismate synthase from2 yersinia pestis co92 Phyre2 PDB 2g5f chain A domain 1 Region: 5 - 390 Aligned: 380 Modelled: 386 Confidence: 100.0% Identity: 21% Fold: ADC synthase Superfamily: ADC synthase Family: ADC synthase Phyre2 PDB 1i1q chain A Region: 18 - 388 Aligned: 361 Modelled: 371 Confidence: 100.0% Identity: 22% Fold: ADC synthase Superfamily: ADC synthase Family: ADC synthase Phyre2 PDB 1i7q chain A Region: 17 - 388 Aligned: 362 Modelled: 372 Confidence: 100.0% Identity: 22% Fold: ADC synthase Superfamily: ADC synthase Family: ADC synthase Phyre2 PDB 3h9m chain A Region: 22 - 388 Aligned: 336 Modelled: 348 Confidence: 100.0% Identity: 17% PDB header:lyase Chain: A: PDB Molecule:p-aminobenzoate synthetase, component i; PDBTitle: crystal structure of para-aminobenzoate synthetase,2 component i from cytophaga hutchinsonii Phyre2 PDB 2i6y chain A Region: 15 - 388 Aligned: 359 Modelled: 374 Confidence: 100.0% Identity: 22% PDB header:lyase Chain: A: PDB Molecule:anthranilate synthase component i, putative; PDBTitle: structure and mechanism of mycobacterium tuberculosis salicylate2 synthase, mbti Phyre2 PDB 3r74 chain B Region: 110 - 390 Aligned: 278 Modelled: 281 Confidence: 100.0% Identity: 21% PDB header:lyase, biosynthetic protein Chain: B: PDB Molecule:anthranilate/para-aminobenzoate synthases component i; PDBTitle: crystal structure of 2-amino-2-desoxyisochorismate synthase (adic)2 synthase phze from burkholderia lata 383 Phyre2 PDB 1k0g chain A Region: 21 - 388 Aligned: 332 Modelled: 335 Confidence: 100.0% Identity: 22% Fold: ADC synthase Superfamily: ADC synthase Family: ADC synthase Phyre2 PDB 3nqk chain A Region: 325 - 361 Aligned: 37 Modelled: 37 Confidence: 41.9% Identity: 16% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:uncharacterized protein; PDBTitle: crystal structure of a structural genomics, unknown function2 (bacova_03322) from bacteroides ovatus at 2.61 a resolution Phyre2 PDB 2ffc chain A domain 1 Region: 302 - 388 Aligned: 82 Modelled: 87 Confidence: 23.0% Identity: 18% Fold: TIM beta/alpha-barrel Superfamily: Ribulose-phoshate binding barrel Family: Decarboxylase Phyre2 PDB 1hxr chain A Region: 329 - 346 Aligned: 18 Modelled: 18 Confidence: 19.4% Identity: 22% Fold: Mss4-like Superfamily: Mss4-like Family: RabGEF Mss4 Phyre2 PDB 2fu5 chain A domain 1 Region: 329 - 346 Aligned: 18 Modelled: 18 Confidence: 19.1% Identity: 22% Fold: Mss4-like Superfamily: Mss4-like Family: RabGEF Mss4 Phyre2 PDB 1qus chain A Region: 324 - 340 Aligned: 17 Modelled: 17 Confidence: 15.2% Identity: 35% Fold: Lysozyme-like Superfamily: Lysozyme-like Family: Bacterial muramidase, catalytic domain Phyre2 PDB 2km1 chain A Region: 294 - 316 Aligned: 23 Modelled: 23 Confidence: 13.8% Identity: 26% PDB header:protein binding Chain: A: PDB Molecule:protein dre2; PDBTitle: solution structure of the n-terminal domain of the yeast protein dre2 Phyre2 PDB 1a6c chain A domain 2 Region: 334 - 369 Aligned: 36 Modelled: 36 Confidence: 12.4% Identity: 11% Fold: Nucleoplasmin-like/VP (viral coat and capsid proteins) Superfamily: Positive stranded ssRNA viruses Family: Comoviridae-like VP Phyre2 1 c3hwoB_ 2 c3os6A_ 3 d3bzna1 4 d1qdla_ 5 d2fn0a1 6 c3gseA_ 7 d2g5fa1 8 d1i1qa_ 9 d1i7qa_ 10 c3h9mA_ 11 c2i6yA_ 12 c3r74B_ 13 d1k0ga_ 14 c3nqkA_ 15 d2ffca1 16 d1hxra_ 17 d2fu5a1 18 d1qusa_ 19 c2km1A_ 20 d1a6ca2 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 c3hwoB_ 100.0 100 PDB header:isomerase Chain: B: PDB Molecule:isochorismate synthase entc; PDBTitle: crystal structure of escherichia coli enterobactin-specific2 isochorismate synthase entc in complex with isochorismate 2 c3os6A_ 100.0 38 PDB header:isomerase Chain: A: PDB Molecule:isochorismate synthase dhbc; PDBTitle: crystal structure of putative 2,3-dihydroxybenzoate-specific2 isochorismate synthase, dhbc from bacillus anthracis. 3 d3bzna1 100.0 27 Fold:ADC synthase Superfamily:ADC synthase Family:ADC synthase 4 d1qdla_ 100.0 25 Fold:ADC synthase Superfamily:ADC synthase Family:ADC synthase 5 d2fn0a1 100.0 22 Fold:ADC synthase Superfamily:ADC synthase Family:ADC synthase 6 c3gseA_ 100.0 24 PDB header:isomerase Chain: A: PDB Molecule:menaquinone-specific isochorismate synthase; PDBTitle: crystal structure of menaquinone-specific isochorismate synthase from2 yersinia pestis co92 7 d2g5fa1 100.0 21 Fold:ADC synthase Superfamily:ADC synthase Family:ADC synthase 8 d1i1qa_ 100.0 22 Fold:ADC synthase Superfamily:ADC synthase Family:ADC synthase 9 d1i7qa_ 100.0 22 Fold:ADC synthase Superfamily:ADC synthase Family:ADC synthase 10 c3h9mA_ 100.0 17 PDB header:lyase Chain: A: PDB Molecule:p-aminobenzoate synthetase, component i; PDBTitle: crystal structure of para-aminobenzoate synthetase,2 component i from cytophaga hutchinsonii 11 c2i6yA_ 100.0 22 PDB header:lyase Chain: A: PDB Molecule:anthranilate synthase component i, putative; PDBTitle: structure and mechanism of mycobacterium tuberculosis salicylate2 synthase, mbti 12 c3r74B_ 100.0 21 PDB header:lyase, biosynthetic protein Chain: B: PDB Molecule:anthranilate/para-aminobenzoate synthases component i; PDBTitle: crystal structure of 2-amino-2-desoxyisochorismate synthase (adic)2 synthase phze from burkholderia lata 383 13 d1k0ga_ 100.0 22 Fold:ADC synthase Superfamily:ADC synthase Family:ADC synthase 14 c3nqkA_ 41.9 16 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:uncharacterized protein; PDBTitle: crystal structure of a structural genomics, unknown function2 (bacova_03322) from bacteroides ovatus at 2.61 a resolution 15 d2ffca1 23.0 18 Fold:TIM beta/alpha-barrel Superfamily:Ribulose-phoshate binding barrel Family:Decarboxylase 16 d1hxra_ 19.4 22 Fold:Mss4-like Superfamily:Mss4-like Family:RabGEF Mss4 17 d2fu5a1 19.1 22 Fold:Mss4-like Superfamily:Mss4-like Family:RabGEF Mss4 18 d1qusa_ 15.2 35 Fold:Lysozyme-like Superfamily:Lysozyme-like Family:Bacterial muramidase, catalytic domain 19 c2km1A_ 13.8 26 PDB header:protein binding Chain: A: PDB Molecule:protein dre2; PDBTitle: solution structure of the n-terminal domain of the yeast protein dre2 20 d1a6ca2 12.4 11 Fold:Nucleoplasmin-like/VP (viral coat and capsid proteins) Superfamily:Positive stranded ssRNA viruses Family:Comoviridae-like VP 21 d1cvra1 not modelled 9.6 11 Fold:Immunoglobulin-like beta-sandwich Superfamily:E set domains Family:Gingipain R (RgpB), C-terminal domain 22 c2z1tA_ not modelled 9.0 21 PDB header:lyase Chain: A: PDB Molecule:hydrogenase expression/formation protein hype; PDBTitle: crystal structure of hydrogenase maturation protein hype 23 c3fiuD_ not modelled 8.7 12 PDB header:ligase Chain: D: PDB Molecule:nh(3)-dependent nad(+) synthetase; PDBTitle: structure of nmn synthetase from francisella tularensis 24 c3fuyC_ not modelled 8.6 30 PDB header:structural genomics, unknown function Chain: C: PDB Molecule:putative integron gene cassette protein; PDBTitle: structure from the mobile metagenome of cole harbour salt2 marsh: integron cassette protein hfx_cass1 25 c1ey2A_ not modelled 8.5 28 PDB header:oxidoreductase Chain: A: PDB Molecule:homogentisate 1,2-dioxygenase; PDBTitle: human homogentisate dioxygenase with fe(ii) 26 d1eyba_ not modelled 8.5 28 Fold:Double-stranded beta-helix Superfamily:RmlC-like cupins Family:Homogentisate dioxygenase 27 d1d5ta2 not modelled 8.1 21 Fold:FAD-linked reductases, C-terminal domain Superfamily:FAD-linked reductases, C-terminal domain Family:GDI-like 28 c1tzsA_ not modelled 7.1 13 PDB header:hydrolase Chain: A: PDB Molecule:cathepsin e; PDBTitle: crystal structure of an activation intermediate of2 cathepsin e 29 d2af7a1 not modelled 7.0 17 Fold:AhpD-like Superfamily:AhpD-like Family:CMD-like 30 d3cmsa_ not modelled 6.3 15 Fold:Acid proteases Superfamily:Acid proteases Family:Pepsin-like 31 c3c66B_ not modelled 6.0 19 PDB header:transferase Chain: B: PDB Molecule:poly(a) polymerase; PDBTitle: yeast poly(a) polymerase in complex with fip1 residues 80-105 32 d2c1wa1 not modelled 6.0 25 Fold:EndoU-like Superfamily:EndoU-like Family:Eukaryotic EndoU ribonuclease 33 c3rrrB_ not modelled 6.0 29 PDB header:viral protein Chain: B: PDB Molecule:fusion glycoprotein f0; PDBTitle: structure of the rsv f protein in the post-fusion conformation 34 d1rd5a_ not modelled 6.0 33 Fold:TIM beta/alpha-barrel Superfamily:Ribulose-phoshate binding barrel Family:Tryptophan biosynthesis enzymes 35 c1wxnA_ not modelled 5.8 31 PDB header:toxin Chain: A: PDB Molecule:toxin apetx2; PDBTitle: solution structure of apetx2, a specific peptide inhibitor2 of asic3 proton-gated channels 36 c3rpdB_ not modelled 5.5 31 PDB header:transferase Chain: B: PDB Molecule:methionine synthase (b12-independent); PDBTitle: the structure of a b12-independent methionine synthase from shewanella2 sp. w3-18-1 in complex with selenomethionine.

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

---

Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0