Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	L	L	H	I	L	Y	L	V	G	I	T	A	E	A	M	T	G	A	L	A	A	G	R	R	R	M	D	T	F	G	V	I	I	I	A	T	A	T	A	I	G	G	G	S	V	R	D	I	L	L	G	H	Y	P	L	G	W	V	K
Secondary structure
SS confidence
Disorder		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		H	P	E	Y	V	I	I	V	A	T	A	A	V	L	T	T	I	V	A	P	V	M	P	Y	L	R	K	V	F	L	V	L	D	A	L	G	L	V	V	F	S	I	I	G	A	Q	V	A	L	D	M	G	H	G	P	I	I	A	V	V
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		A	A	V	T	T	G	V	F	G	G	V	L	R	D	M	F	C	K	R	I	P	L	V	F	Q	K	E	L	Y	A	G	V	S	F	A	S	A	V	L	Y	I	A	L	Q	H	Y	V	S	N	H	D	V	V	I	I	S	T	L	V	F
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.
Sequence		G	F	F	A	R	L	L	A	L	R	L	K	L	G	L	P	V	F	Y	Y	S	H	E	G	H
Secondary structure
SS confidence
Disorder																				?	?	?	?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 1vfg chain A domain 2 Region: 43 - 55 Aligned: 13 Modelled: 13 Confidence: 72.9% Identity: 69% Fold: Nucleotidyltransferase Superfamily: Nucleotidyltransferase Family: Poly A polymerase head domain-like Phyre2 PDB 1miw chain A domain 2 Region: 43 - 55 Aligned: 13 Modelled: 13 Confidence: 71.2% Identity: 62% Fold: Nucleotidyltransferase Superfamily: Nucleotidyltransferase Family: Poly A polymerase head domain-like Phyre2 PDB 1vfg chain B Region: 43 - 55 Aligned: 13 Modelled: 13 Confidence: 70.2% Identity: 69% PDB header:transferase/rna Chain: B: PDB Molecule:poly a polymerase; PDBTitle: crystal structure of trna nucleotidyltransferase complexed2 with a primer trna and an incoming atp analog Phyre2 PDB 3aqn chain A Region: 43 - 55 Aligned: 13 Modelled: 13 Confidence: 65.4% Identity: 69% PDB header:transferase Chain: A: PDB Molecule:poly(a) polymerase; PDBTitle: complex structure of bacterial protein (apo form ii) Phyre2 PDB 3h37 chain B Region: 43 - 55 Aligned: 13 Modelled: 13 Confidence: 64.8% Identity: 62% PDB header:transferase Chain: B: PDB Molecule:trna nucleotidyl transferase-related protein; PDBTitle: the structure of cca-adding enzyme apo form i Phyre2 PDB 1ou5 chain A Region: 43 - 55 Aligned: 13 Modelled: 13 Confidence: 59.5% Identity: 62% PDB header:translation, transferase Chain: A: PDB Molecule:trna cca-adding enzyme; PDBTitle: crystal structure of human cca-adding enzyme Phyre2 PDB 1ou5 chain A domain 2 Region: 43 - 55 Aligned: 13 Modelled: 13 Confidence: 59.5% Identity: 62% Fold: Nucleotidyltransferase Superfamily: Nucleotidyltransferase Family: Poly A polymerase head domain-like Phyre2 PDB 1miy chain B Region: 43 - 55 Aligned: 13 Modelled: 13 Confidence: 56.3% Identity: 62% PDB header:translation, transferase Chain: B: PDB Molecule:trna cca-adding enzyme; PDBTitle: crystal structure of bacillus stearothermophilus cca-adding enzyme in2 complex with ctp Phyre2 PDB 1t3t chain A domain 4 Region: 110 - 137 Aligned: 27 Modelled: 28 Confidence: 48.7% Identity: 30% Fold: Bacillus chorismate mutase-like Superfamily: PurM N-terminal domain-like Family: PurM N-terminal domain-like Phyre2 PDB 1t3t chain A Region: 110 - 137 Aligned: 27 Modelled: 28 Confidence: 25.7% Identity: 26% PDB header:ligase Chain: A: PDB Molecule:phosphoribosylformylglycinamidine synthase; PDBTitle: structure of formylglycinamide synthetase Phyre2 PDB 3d54 chain I Region: 111 - 137 Aligned: 26 Modelled: 27 Confidence: 23.9% Identity: 35% PDB header:ligase Chain: I: PDB Molecule:phosphoribosylformylglycinamidine synthase ii; PDBTitle: stucture of purlqs from thermotoga maritima Phyre2 PDB 2hs0 chain A Region: 111 - 137 Aligned: 26 Modelled: 27 Confidence: 22.8% Identity: 35% PDB header:ligase Chain: A: PDB Molecule:phosphoribosylformylglycinamidine synthase ii; PDBTitle: t. maritima purl complexed with atp Phyre2 PDB 2dxb chain R Region: 50 - 64 Aligned: 14 Modelled: 15 Confidence: 21.2% Identity: 36% PDB header:hydrolase Chain: R: PDB Molecule:thiocyanate hydrolase subunit gamma; PDBTitle: recombinant thiocyanate hydrolase comprising partially-modified cobalt2 centers Phyre2 PDB 1v29 chain A Region: 50 - 64 Aligned: 14 Modelled: 15 Confidence: 21.2% Identity: 57% Fold: Nitrile hydratase alpha chain Superfamily: Nitrile hydratase alpha chain Family: Nitrile hydratase alpha chain Phyre2 PDB 1vk3 chain A domain 1 Region: 111 - 137 Aligned: 26 Modelled: 27 Confidence: 19.9% Identity: 35% Fold: Bacillus chorismate mutase-like Superfamily: PurM N-terminal domain-like Family: PurM N-terminal domain-like Phyre2 PDB 3qyh chain G Region: 50 - 64 Aligned: 14 Modelled: 15 Confidence: 19.2% Identity: 43% PDB header:lyase Chain: G: PDB Molecule:co-type nitrile hydratase alpha subunit; PDBTitle: crystal structure of co-type nitrile hydratase beta-h71l from2 pseudomonas putida. Phyre2 PDB 2qdy chain A domain 1 Region: 50 - 64 Aligned: 14 Modelled: 15 Confidence: 18.6% Identity: 50% Fold: Nitrile hydratase alpha chain Superfamily: Nitrile hydratase alpha chain Family: Nitrile hydratase alpha chain Phyre2 PDB 1ugp chain A Region: 50 - 64 Aligned: 14 Modelled: 15 Confidence: 18.3% Identity: 50% Fold: Nitrile hydratase alpha chain Superfamily: Nitrile hydratase alpha chain Family: Nitrile hydratase alpha chain Phyre2 PDB 3id6 chain A Region: 190 - 196 Aligned: 7 Modelled: 7 Confidence: 12.5% Identity: 14% PDB header:transferase Chain: A: PDB Molecule:pre mrna splicing protein; PDBTitle: crystal structure of sulfolobus solfataricus nop5 (1-262) and2 fibrillarin complex Phyre2 PDB 3ac6 chain A Region: 111 - 137 Aligned: 26 Modelled: 25 Confidence: 12.4% Identity: 35% PDB header:ligase Chain: A: PDB Molecule:phosphoribosylformylglycinamidine synthase 2; PDBTitle: crystal structure of purl from thermus thermophilus Phyre2 1 d1vfga2 2 d1miwa2 3 c1vfgB_ 4 c3aqnA_ 5 c3h37B_ 6 c1ou5A_ 7 d1ou5a2 8 c1miyB_ 9 d1t3ta4 10 c1t3tA_ 11 c3d54I_ 12 c2hs0A_ 13 c2dxbR_ 14 d1v29a_ 15 d1vk3a1 16 c3qyhG_ 17 d2qdya1 18 d1ugpa_ 19 c3id6A_ 20 c3ac6A_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 d1vfga2 72.9 69 Fold:Nucleotidyltransferase Superfamily:Nucleotidyltransferase Family:Poly A polymerase head domain-like 2 d1miwa2 71.2 62 Fold:Nucleotidyltransferase Superfamily:Nucleotidyltransferase Family:Poly A polymerase head domain-like 3 c1vfgB_ 70.2 69 PDB header:transferase/rna Chain: B: PDB Molecule:poly a polymerase; PDBTitle: crystal structure of trna nucleotidyltransferase complexed2 with a primer trna and an incoming atp analog 4 c3aqnA_ 65.4 69 PDB header:transferase Chain: A: PDB Molecule:poly(a) polymerase; PDBTitle: complex structure of bacterial protein (apo form ii) 5 c3h37B_ 64.8 62 PDB header:transferase Chain: B: PDB Molecule:trna nucleotidyl transferase-related protein; PDBTitle: the structure of cca-adding enzyme apo form i 6 c1ou5A_ 59.5 62 PDB header:translation, transferase Chain: A: PDB Molecule:trna cca-adding enzyme; PDBTitle: crystal structure of human cca-adding enzyme 7 d1ou5a2 59.5 62 Fold:Nucleotidyltransferase Superfamily:Nucleotidyltransferase Family:Poly A polymerase head domain-like 8 c1miyB_ 56.3 62 PDB header:translation, transferase Chain: B: PDB Molecule:trna cca-adding enzyme; PDBTitle: crystal structure of bacillus stearothermophilus cca-adding enzyme in2 complex with ctp 9 d1t3ta4 48.7 30 Fold:Bacillus chorismate mutase-like Superfamily:PurM N-terminal domain-like Family:PurM N-terminal domain-like 10 c1t3tA_ 25.7 26 PDB header:ligase Chain: A: PDB Molecule:phosphoribosylformylglycinamidine synthase; PDBTitle: structure of formylglycinamide synthetase 11 c3d54I_ 23.9 35 PDB header:ligase Chain: I: PDB Molecule:phosphoribosylformylglycinamidine synthase ii; PDBTitle: stucture of purlqs from thermotoga maritima 12 c2hs0A_ 22.8 35 PDB header:ligase Chain: A: PDB Molecule:phosphoribosylformylglycinamidine synthase ii; PDBTitle: t. maritima purl complexed with atp 13 c2dxbR_ 21.2 36 PDB header:hydrolase Chain: R: PDB Molecule:thiocyanate hydrolase subunit gamma; PDBTitle: recombinant thiocyanate hydrolase comprising partially-modified cobalt2 centers 14 d1v29a_ 21.2 57 Fold:Nitrile hydratase alpha chain Superfamily:Nitrile hydratase alpha chain Family:Nitrile hydratase alpha chain 15 d1vk3a1 19.9 35 Fold:Bacillus chorismate mutase-like Superfamily:PurM N-terminal domain-like Family:PurM N-terminal domain-like 16 c3qyhG_ 19.2 43 PDB header:lyase Chain: G: PDB Molecule:co-type nitrile hydratase alpha subunit; PDBTitle: crystal structure of co-type nitrile hydratase beta-h71l from2 pseudomonas putida. 17 d2qdya1 18.6 50 Fold:Nitrile hydratase alpha chain Superfamily:Nitrile hydratase alpha chain Family:Nitrile hydratase alpha chain 18 d1ugpa_ 18.3 50 Fold:Nitrile hydratase alpha chain Superfamily:Nitrile hydratase alpha chain Family:Nitrile hydratase alpha chain 19 c3id6A_ 12.5 14 PDB header:transferase Chain: A: PDB Molecule:pre mrna splicing protein; PDBTitle: crystal structure of sulfolobus solfataricus nop5 (1-262) and2 fibrillarin complex 20 c3ac6A_ 12.4 35 PDB header:ligase Chain: A: PDB Molecule:phosphoribosylformylglycinamidine synthase 2; PDBTitle: crystal structure of purl from thermus thermophilus 21 d1otsa_ not modelled 11.7 16 Fold:Clc chloride channel Superfamily:Clc chloride channel Family:Clc chloride channel 22 d1h9aa2 not modelled 8.7 44 Fold:FwdE/GAPDH domain-like Superfamily:Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain Family:Glucose 6-phosphate dehydrogenase-like 23 c2qveA_ not modelled 8.7 29 PDB header:transferase Chain: A: PDB Molecule:tyrosine aminomutase; PDBTitle: crystal structure of sgtam bound to mechanism based inhibitor 24 d1qkia2 not modelled 8.2 33 Fold:FwdE/GAPDH domain-like Superfamily:Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain Family:Glucose 6-phosphate dehydrogenase-like 25 d1gkma_ not modelled 7.3 20 Fold:L-aspartase-like Superfamily:L-aspartase-like Family:HAL/PAL-like 26 c2nynD_ not modelled 6.2 17 PDB header:lyase Chain: D: PDB Molecule:phenylalanine/histidine ammonia-lyase; PDBTitle: crystal structure of phenylalanine ammonia-lyase from2 anabaena variabilis 27 c2nnwC_ not modelled 6.1 29 PDB header:transferase Chain: C: PDB Molecule:nop5/nop56 related protein; PDBTitle: alternative conformations of nop56/58-fibrillarin complex and2 implication for induced-fit assenly of box c/d rnps 28 c2o6yF_ not modelled 6.0 22 PDB header:lyase Chain: F: PDB Molecule:putative histidine ammonia-lyase; PDBTitle: tyrosine ammonia-lyase from rhodobacter sphaeroides 29 c1fw5A_ not modelled 6.0 14 PDB header:viral protein Chain: A: PDB Molecule:nonstructural protein nsp1; PDBTitle: solution structure of membrane binding peptide of semliki2 forest virus mrna capping enzyme nsp1 30 c1flcB_ not modelled 5.9 25 PDB header:hydrolase Chain: B: PDB Molecule:haemagglutinin-esterase-fusion glycoprotein; PDBTitle: x-ray structure of the haemagglutinin-esterase-fusion glycoprotein of2 influenza c virus 31 c1qkiE_ not modelled 5.8 33 PDB header:oxidoreductase Chain: E: PDB Molecule:glucose-6-phosphate 1-dehydrogenase; PDBTitle: x-ray structure of human glucose 6-phosphate dehydrogenase2 (variant canton r459l) complexed with structural nadp+ 32 d1rxta2 not modelled 5.5 14 Fold:Acyl-CoA N-acyltransferases (Nat) Superfamily:Acyl-CoA N-acyltransferases (Nat) Family:N-myristoyl transferase, NMT 33 c3p5nA_ not modelled 5.3 9 PDB header:transport protein Chain: A: PDB Molecule:riboflavin uptake protein; PDBTitle: structure and mechanism of the s component of a bacterial ecf2 transporter 34 c2bhlB_ not modelled 5.3 33 PDB header:oxidoreductase (choh(d)-nadp) Chain: B: PDB Molecule:glucose-6-phosphate 1-dehydrogenase; PDBTitle: x-ray structure of human glucose-6-phosphate dehydrogenase2 (deletion variant) complexed with glucose-6-phosphate 35 c1h9aA_ not modelled 5.1 44 PDB header:oxidoreductase (choh(d) - nad(p)) Chain: A: PDB Molecule:glucose 6-phosphate 1-dehydrogenase; PDBTitle: complex of active mutant (q365->c) of glucose 6-phosphate2 dehydrogenase from l. mesenteroides with coenzyme nadp

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

Transmembrane helix prediction

Transmembrane helices have been predicted in your sequence to adopt the topology shown below

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0