Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	W	V	N	K	Y	I	D	D	C	T	D	E	D	L	N	D	R	D	F	I	A	S	V	V	D	R	A	I	F	H	F	A	I	N	S	I	C	N	P	G	D	N	K	D	A	M	P	I	E	Q	C	T	F	D	V	E	T	K	N
Secondary structure
SS confidence
Disorder		?	?										?		?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		D	L	P	S	T	V	Q	L	F	Y	E	E	S	K	D	N	E	P	L	A	N	I	H	F	Q	A	I	G	S	G	F	L	T	F	V	N	A	C	Q	E	H	D	D	N	S	L	K	L	F	A	S	L	L	I	S	L	S	Y	S	S
Secondary structure
SS confidence
Disorder																																									?		?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		A	Y	A	D	L	S	E	T	V	Y	I	N	E	N	N	E	S	Y	L	K	A	Q	F	E	K	L	S	Q	R	D	M	K	K	Y	L	G	E	M	K	R	L	A	D	G	G	E	M	N	F	D	G	Y	L	D	K	M	S	H	L	V
Secondary structure
SS confidence
Disorder																																										?		?			?													?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.
Sequence		N	E	G	T	L	D	P	D	I	L	S	K	M	R	D	A	A	P	Q	L	I	S	F	A	K	S	F	D	P	T	S	K	E	E	I	K	I	L	T	D	T	S	K	L	I	Y	D	L	F	G	V	K	S	E	K
Secondary structure
SS confidence
Disorder		?	?	?	?	?										?	?																?																			?	?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 2k5e chain A Region: 63 - 112 Aligned: 40 Modelled: 50 Confidence: 28.3% Identity: 18% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:uncharacterized protein; PDBTitle: solution structure of putative uncharacterized protein2 gsu1278 from methanocaldococcus jannaschii, northeast3 structural genomics consortium (nesg) target gsr195 Phyre2 PDB 2uub chain E domain 1 Region: 124 - 160 Aligned: 37 Modelled: 37 Confidence: 20.1% Identity: 19% Fold: Ribosomal protein S5 domain 2-like Superfamily: Ribosomal protein S5 domain 2-like Family: Translational machinery components Phyre2 PDB 2pr1 chain B Region: 194 - 207 Aligned: 14 Modelled: 14 Confidence: 18.0% Identity: 50% PDB header:transferase Chain: B: PDB Molecule:uncharacterized n-acetyltransferase ylbp; PDBTitle: crystal structure of the bacillus subtilis n-acetyltransferase ylbp2 protein in complex with coenzyme-a Phyre2 PDB 1y6z chain A Region: 74 - 195 Aligned: 86 Modelled: 86 Confidence: 13.6% Identity: 16% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:heat shock protein, putative; PDBTitle: middle domain of plasmodium falciparum putative heat shock protein2 pf14_0417 Phyre2 PDB 2jug chain B Region: 180 - 217 Aligned: 38 Modelled: 38 Confidence: 11.9% Identity: 21% PDB header:biosynthetic protein Chain: B: PDB Molecule:tubc protein; PDBTitle: multienzyme docking in hybrid megasynthetases Phyre2 PDB 2gu2 chain A domain 1 Region: 180 - 214 Aligned: 35 Modelled: 35 Confidence: 11.1% Identity: 23% Fold: Phosphorylase/hydrolase-like Superfamily: Zn-dependent exopeptidases Family: AstE/AspA-like Phyre2 PDB 1ci4 chain A Region: 35 - 47 Aligned: 13 Modelled: 13 Confidence: 10.4% Identity: 15% Fold: SAM domain-like Superfamily: Barrier-to-autointegration factor, BAF Family: Barrier-to-autointegration factor, BAF Phyre2 PDB 2f2i chain A Region: 52 - 63 Aligned: 12 Modelled: 12 Confidence: 10.1% Identity: 50% PDB header:antimicrobial protein Chain: A: PDB Molecule:kalata-b1; PDBTitle: solution structure of [p20d,v21k]-kalata b1 Phyre2 PDB 2kn8 chain A Region: 120 - 148 Aligned: 29 Modelled: 29 Confidence: 9.4% Identity: 24% PDB header:protein binding, dna binding protein Chain: A: PDB Molecule:dna cleavage and packaging protein large subunit, ul89; PDBTitle: nmr structure of the c-terminal domain of pul89 Phyre2 PDB 3elg chain A domain 1 Region: 55 - 82 Aligned: 28 Modelled: 28 Confidence: 9.1% Identity: 29% Fold: BLIP-like Superfamily: BT0923-like Family: BT0923-like Phyre2 PDB 3mdj chain B Region: 130 - 215 Aligned: 81 Modelled: 86 Confidence: 8.9% Identity: 19% PDB header:hydrolase/hydrolase inhibitor Chain: B: PDB Molecule:endoplasmic reticulum aminopeptidase 1; PDBTitle: er aminopeptidase, erap1, bound to the zinc aminopeptidase inhibitor,2 bestatin Phyre2 PDB 1kdx chain B Region: 144 - 158 Aligned: 15 Modelled: 15 Confidence: 8.4% Identity: 40% PDB header:transcription regulation complex Chain: B: PDB Molecule:creb; PDBTitle: kix domain of mouse cbp (creb binding protein) in complex2 with phosphorylated kinase inducible domain (pkid) of rat3 creb (cyclic amp response element binding protein), nmr 174 structures Phyre2 PDB 1cuk chain A domain 1 Region: 113 - 144 Aligned: 32 Modelled: 32 Confidence: 8.2% Identity: 19% Fold: RuvA C-terminal domain-like Superfamily: DNA helicase RuvA subunit, C-terminal domain Family: DNA helicase RuvA subunit, C-terminal domain Phyre2 PDB 3e4h chain A Region: 52 - 63 Aligned: 12 Modelled: 12 Confidence: 7.8% Identity: 42% PDB header:plant protein Chain: A: PDB Molecule:varv peptide f; PDBTitle: crystal structure of the cyclotide varv f Phyre2 PDB 2jwm chain A Region: 52 - 63 Aligned: 12 Modelled: 12 Confidence: 7.6% Identity: 33% PDB header:plant protein Chain: A: PDB Molecule:kalata-b7; PDBTitle: nmr spatial srtucture of ternary complex kalata b7/mn2+/dpc2 micelle Phyre2 PDB 3di3 chain A Region: 81 - 112 Aligned: 32 Modelled: 32 Confidence: 7.5% Identity: 19% PDB header:cytokine/cytokine receptor Chain: A: PDB Molecule:interleukin-7; PDBTitle: crystal structure of the complex of human interleukin-7 with2 glycosylated human interleukin-7 receptor alpha ectodomain Phyre2 PDB 3due chain A domain 1 Region: 56 - 83 Aligned: 28 Modelled: 25 Confidence: 6.8% Identity: 25% Fold: BLIP-like Superfamily: BT0923-like Family: BT0923-like Phyre2 PDB 2qfa chain C Region: 34 - 45 Aligned: 11 Modelled: 12 Confidence: 6.6% Identity: 55% PDB header:cell cycle/cell cycle/cell cycle Chain: C: PDB Molecule:inner centromere protein; PDBTitle: crystal structure of a survivin-borealin-incenp core complex Phyre2 PDB 2kzv chain A Region: 173 - 213 Aligned: 41 Modelled: 41 Confidence: 6.5% Identity: 20% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:uncharacterized protein; PDBTitle: solution nmr structure of cv_0373(175-257) protein from2 chromobacterium violaceum, northeast structural genomics consortium3 target cvr118a Phyre2 PDB 3rjo chain A Region: 130 - 235 Aligned: 99 Modelled: 106 Confidence: 6.3% Identity: 17% PDB header:hydrolase Chain: A: PDB Molecule:endoplasmic reticulum aminopeptidase 1; PDBTitle: crystal structure of erap1 peptide binding domain Phyre2 1 c2k5eA_ 2 d2uube1 3 c2pr1B_ 4 c1y6zA_ 5 c2jugB_ 6 d2gu2a1 7 d1ci4a_ 8 c2f2iA_ 9 c2kn8A_ 10 d3elga1 11 c3mdjB_ 12 c1kdxB_ 13 d1cuka1 14 c3e4hA_ 15 c2jwmA_ 16 c3di3A_ 17 d3duea1 18 c2qfaC_ 19 c2kzvA_ 20 c3rjoA_ 21 22 23 24 25

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 c2k5eA_ 28.3 18 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:uncharacterized protein; PDBTitle: solution structure of putative uncharacterized protein2 gsu1278 from methanocaldococcus jannaschii, northeast3 structural genomics consortium (nesg) target gsr195 2 d2uube1 20.1 19 Fold:Ribosomal protein S5 domain 2-like Superfamily:Ribosomal protein S5 domain 2-like Family:Translational machinery components 3 c2pr1B_ 18.0 50 PDB header:transferase Chain: B: PDB Molecule:uncharacterized n-acetyltransferase ylbp; PDBTitle: crystal structure of the bacillus subtilis n-acetyltransferase ylbp2 protein in complex with coenzyme-a 4 c1y6zA_ 13.6 16 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:heat shock protein, putative; PDBTitle: middle domain of plasmodium falciparum putative heat shock protein2 pf14_0417 5 c2jugB_ 11.9 21 PDB header:biosynthetic protein Chain: B: PDB Molecule:tubc protein; PDBTitle: multienzyme docking in hybrid megasynthetases 6 d2gu2a1 11.1 23 Fold:Phosphorylase/hydrolase-like Superfamily:Zn-dependent exopeptidases Family:AstE/AspA-like 7 d1ci4a_ 10.4 15 Fold:SAM domain-like Superfamily:Barrier-to-autointegration factor, BAF Family:Barrier-to-autointegration factor, BAF 8 c2f2iA_ 10.1 50 PDB header:antimicrobial protein Chain: A: PDB Molecule:kalata-b1; PDBTitle: solution structure of [p20d,v21k]-kalata b1 9 c2kn8A_ 9.4 24 PDB header:protein binding, dna binding protein Chain: A: PDB Molecule:dna cleavage and packaging protein large subunit, ul89; PDBTitle: nmr structure of the c-terminal domain of pul89 10 d3elga1 9.1 29 Fold:BLIP-like Superfamily:BT0923-like Family:BT0923-like 11 c3mdjB_ 8.9 19 PDB header:hydrolase/hydrolase inhibitor Chain: B: PDB Molecule:endoplasmic reticulum aminopeptidase 1; PDBTitle: er aminopeptidase, erap1, bound to the zinc aminopeptidase inhibitor,2 bestatin 12 c1kdxB_ 8.4 40 PDB header:transcription regulation complex Chain: B: PDB Molecule:creb; PDBTitle: kix domain of mouse cbp (creb binding protein) in complex2 with phosphorylated kinase inducible domain (pkid) of rat3 creb (cyclic amp response element binding protein), nmr 174 structures 13 d1cuka1 8.2 19 Fold:RuvA C-terminal domain-like Superfamily:DNA helicase RuvA subunit, C-terminal domain Family:DNA helicase RuvA subunit, C-terminal domain 14 c3e4hA_ 7.8 42 PDB header:plant protein Chain: A: PDB Molecule:varv peptide f; PDBTitle: crystal structure of the cyclotide varv f 15 c2jwmA_ 7.6 33 PDB header:plant protein Chain: A: PDB Molecule:kalata-b7; PDBTitle: nmr spatial srtucture of ternary complex kalata b7/mn2+/dpc2 micelle 16 c3di3A_ 7.5 19 PDB header:cytokine/cytokine receptor Chain: A: PDB Molecule:interleukin-7; PDBTitle: crystal structure of the complex of human interleukin-7 with2 glycosylated human interleukin-7 receptor alpha ectodomain 17 d3duea1 6.8 25 Fold:BLIP-like Superfamily:BT0923-like Family:BT0923-like 18 c2qfaC_ 6.6 55 PDB header:cell cycle/cell cycle/cell cycle Chain: C: PDB Molecule:inner centromere protein; PDBTitle: crystal structure of a survivin-borealin-incenp core complex 19 c2kzvA_ 6.5 20 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:uncharacterized protein; PDBTitle: solution nmr structure of cv_0373(175-257) protein from2 chromobacterium violaceum, northeast structural genomics consortium3 target cvr118a 20 c3rjoA_ 6.3 17 PDB header:hydrolase Chain: A: PDB Molecule:endoplasmic reticulum aminopeptidase 1; PDBTitle: crystal structure of erap1 peptide binding domain 21 d1pt4a_ not modelled 6.3 42 Fold:Knottins (small inhibitors, toxins, lectins) Superfamily:Cyclotides Family:Kalata B1 22 d1tyka_ not modelled 5.7 44 Fold:Knottins (small inhibitors, toxins, lectins) Superfamily:omega toxin-like Family:Spider toxins 23 d1nb1a_ not modelled 5.5 50 Fold:Knottins (small inhibitors, toxins, lectins) Superfamily:Cyclotides Family:Kalata B1 24 d2e1ba1 not modelled 5.4 24 Fold:Reductase/isomerase/elongation factor common domain Superfamily:Translation proteins Family:AlaX-M N-terminal domain-like 25 c1k0rB_ not modelled 5.3 13 PDB header:transcription Chain: B: PDB Molecule:nusa; PDBTitle: crystal structure of mycobacterium tuberculosis nusa

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

Transmembrane helix prediction

Transmembrane helices have been predicted in your sequence to adopt the topology shown below

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0