Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	D	K	Q	S	L	H	E	T	A	K	R	L	A	L	E	L	P	F	V	E	L	C	W	P	F	G	P	E	F	D	V	F	K	I	G	G	K	I	F	M	L	S	S	E	L	R	G	V	P	F	I	N	L	K	S	D	P	Q	K
Secondary structure
SS confidence
Disorder		?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		S	L	L	N	Q	Q	I	Y	P	S	I	K	P	G	Y	H	M	N	K	K	H	W	I	S	V	Y	P	G	E	E	I	S	E	A	L	L	R	D	L	I	N	D	S	W	N	L	V	V	D	G	L	A	K	R	D	Q	K	R	V	R
Secondary structure
SS confidence
Disorder																																																											?	?	?
Disorder confidence
		.	.
Sequence		P	G
Secondary structure
SS confidence
Disorder		?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 2fki chain A domain 1 Region: 1 - 119 Aligned: 115 Modelled: 119 Confidence: 100.0% Identity: 23% Fold: Secretion chaperone-like Superfamily: YjbR-like Family: YjbR-like Phyre2 PDB 2a1v chain A domain 1 Region: 7 - 119 Aligned: 113 Modelled: 113 Confidence: 100.0% Identity: 33% Fold: Secretion chaperone-like Superfamily: YjbR-like Family: YjbR-like Phyre2 PDB 3h9x chain B Region: 1 - 107 Aligned: 102 Modelled: 106 Confidence: 100.0% Identity: 28% PDB header:structural genomics, unknown function Chain: B: PDB Molecule:uncharacterized protein pspto_3016; PDBTitle: crystal structure of the pspto_3016 protein from2 pseudomonas syringae, northeast structural genomics3 consortium target psr293 Phyre2 PDB 2oc6 chain A domain 1 Region: 3 - 103 Aligned: 94 Modelled: 101 Confidence: 97.3% Identity: 13% Fold: Secretion chaperone-like Superfamily: YdhG-like Family: YdhG-like Phyre2 PDB 2i8d chain A domain 1 Region: 3 - 103 Aligned: 93 Modelled: 101 Confidence: 97.2% Identity: 14% Fold: Secretion chaperone-like Superfamily: YdhG-like Family: YdhG-like Phyre2 PDB 2od0 chain A domain 1 Region: 6 - 107 Aligned: 95 Modelled: 102 Confidence: 96.8% Identity: 17% Fold: Secretion chaperone-like Superfamily: YgaC/TfoX-N like Family: TfoX N-terminal domain-like Phyre2 PDB 2kl4 chain A Region: 3 - 103 Aligned: 94 Modelled: 99 Confidence: 96.6% Identity: 13% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:bh2032 protein; PDBTitle: nmr structure of the protein nb7804a Phyre2 PDB 3n0k chain A Region: 28 - 83 Aligned: 49 Modelled: 56 Confidence: 39.2% Identity: 14% PDB header:hydrolase inhibitor Chain: A: PDB Molecule:serine protease inhibitor 1; PDBTitle: proteinase inhibitor from coprinopsis cinerea Phyre2 PDB 3s83 chain A Region: 57 - 104 Aligned: 38 Modelled: 48 Confidence: 21.5% Identity: 11% PDB header:signaling protein Chain: A: PDB Molecule:ggdef family protein; PDBTitle: crystal structure of eal domain from caulobacter crescentus cb15 Phyre2 PDB 2j7q chain A domain 1 Region: 31 - 105 Aligned: 75 Modelled: 75 Confidence: 21.2% Identity: 11% Fold: Cysteine proteinases Superfamily: Cysteine proteinases Family: M48USP-like Phyre2 PDB 2rax chain Y Region: 94 - 117 Aligned: 24 Modelled: 24 Confidence: 18.0% Identity: 17% PDB header:cell cycle Chain: Y: PDB Molecule:borealin; PDBTitle: crystal structure of borealin (20-78) bound to survivin (1-120) Phyre2 PDB 1lq9 chain A Region: 51 - 92 Aligned: 42 Modelled: 42 Confidence: 15.7% Identity: 5% Fold: Ferredoxin-like Superfamily: Dimeric alpha+beta barrel Family: Actinorhodin biosynthesis monooxygenase ActVa-Orf6 Phyre2 PDB 1pu1 chain A Region: 83 - 105 Aligned: 23 Modelled: 23 Confidence: 14.6% Identity: 4% Fold: Hypothetical protein MTH677 Superfamily: Hypothetical protein MTH677 Family: Hypothetical protein MTH677 Phyre2 PDB 1xx6 chain A domain 1 Region: 75 - 110 Aligned: 35 Modelled: 36 Confidence: 12.7% Identity: 14% Fold: P-loop containing nucleoside triphosphate hydrolases Superfamily: P-loop containing nucleoside triphosphate hydrolases Family: Type II thymidine kinase Phyre2 PDB 2i0k chain A domain 1 Region: 75 - 122 Aligned: 46 Modelled: 48 Confidence: 11.8% Identity: 11% Fold: Ferredoxin-like Superfamily: FAD-linked oxidases, C-terminal domain Family: Cholesterol oxidase Phyre2 PDB 3cb4 chain D Region: 3 - 56 Aligned: 53 Modelled: 54 Confidence: 11.5% Identity: 13% PDB header:translation Chain: D: PDB Molecule:gtp-binding protein lepa; PDBTitle: the crystal structure of lepa Phyre2 PDB 1ybx chain A Region: 78 - 121 Aligned: 44 Modelled: 44 Confidence: 10.1% Identity: 16% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:conserved hypothetical protein; PDBTitle: conserved hypothetical protein cth-383 from clostridium thermocellum Phyre2 PDB 1ign chain A domain 1 Region: 93 - 120 Aligned: 28 Modelled: 28 Confidence: 7.9% Identity: 7% Fold: DNA/RNA-binding 3-helical bundle Superfamily: Homeodomain-like Family: DNA-binding domain of rap1 Phyre2 PDB 1gmu chain B Region: 42 - 101 Aligned: 60 Modelled: 60 Confidence: 7.0% Identity: 17% PDB header:metallochaperone Chain: B: PDB Molecule:uree; PDBTitle: structure of uree Phyre2 PDB 1nyr chain A domain 3 Region: 61 - 112 Aligned: 52 Modelled: 52 Confidence: 6.9% Identity: 12% Fold: RRF/tRNA synthetase additional domain-like Superfamily: ThrRS/AlaRS common domain Family: Threonyl-tRNA synthetase (ThrRS), second 'additional' domain Phyre2 1 d2fkia1 2 d2a1va1 3 c3h9xB_ 4 d2oc6a1 5 d2i8da1 6 d2od0a1 7 c2kl4A_ 8 c3n0kA_ 9 c3s83A_ 10 d2j7qa1 11 c2raxY_ 12 d1lq9a_ 13 d1pu1a_ 14 d1xx6a1 15 d2i0ka1 16 c3cb4D_ 17 c1ybxA_ 18 d1igna1 19 c1gmuB_ 20 d1nyra3 21 22 23 24 25 26 27 28 29 30 31 32 33 34

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 d2fkia1 100.0 23 Fold:Secretion chaperone-like Superfamily:YjbR-like Family:YjbR-like 2 d2a1va1 100.0 33 Fold:Secretion chaperone-like Superfamily:YjbR-like Family:YjbR-like 3 c3h9xB_ 100.0 28 PDB header:structural genomics, unknown function Chain: B: PDB Molecule:uncharacterized protein pspto_3016; PDBTitle: crystal structure of the pspto_3016 protein from2 pseudomonas syringae, northeast structural genomics3 consortium target psr293 4 d2oc6a1 97.3 13 Fold:Secretion chaperone-like Superfamily:YdhG-like Family:YdhG-like 5 d2i8da1 97.2 14 Fold:Secretion chaperone-like Superfamily:YdhG-like Family:YdhG-like 6 d2od0a1 96.8 17 Fold:Secretion chaperone-like Superfamily:YgaC/TfoX-N like Family:TfoX N-terminal domain-like 7 c2kl4A_ 96.6 13 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:bh2032 protein; PDBTitle: nmr structure of the protein nb7804a 8 c3n0kA_ 39.2 14 PDB header:hydrolase inhibitor Chain: A: PDB Molecule:serine protease inhibitor 1; PDBTitle: proteinase inhibitor from coprinopsis cinerea 9 c3s83A_ 21.5 11 PDB header:signaling protein Chain: A: PDB Molecule:ggdef family protein; PDBTitle: crystal structure of eal domain from caulobacter crescentus cb15 10 d2j7qa1 21.2 11 Fold:Cysteine proteinases Superfamily:Cysteine proteinases Family:M48USP-like 11 c2raxY_ 18.0 17 PDB header:cell cycle Chain: Y: PDB Molecule:borealin; PDBTitle: crystal structure of borealin (20-78) bound to survivin (1-120) 12 d1lq9a_ 15.7 5 Fold:Ferredoxin-like Superfamily:Dimeric alpha+beta barrel Family:Actinorhodin biosynthesis monooxygenase ActVa-Orf6 13 d1pu1a_ 14.6 4 Fold:Hypothetical protein MTH677 Superfamily:Hypothetical protein MTH677 Family:Hypothetical protein MTH677 14 d1xx6a1 12.7 14 Fold:P-loop containing nucleoside triphosphate hydrolases Superfamily:P-loop containing nucleoside triphosphate hydrolases Family:Type II thymidine kinase 15 d2i0ka1 11.8 11 Fold:Ferredoxin-like Superfamily:FAD-linked oxidases, C-terminal domain Family:Cholesterol oxidase 16 c3cb4D_ 11.5 13 PDB header:translation Chain: D: PDB Molecule:gtp-binding protein lepa; PDBTitle: the crystal structure of lepa 17 c1ybxA_ 10.1 16 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:conserved hypothetical protein; PDBTitle: conserved hypothetical protein cth-383 from clostridium thermocellum 18 d1igna1 7.9 7 Fold:DNA/RNA-binding 3-helical bundle Superfamily:Homeodomain-like Family:DNA-binding domain of rap1 19 c1gmuB_ 7.0 17 PDB header:metallochaperone Chain: B: PDB Molecule:uree; PDBTitle: structure of uree 20 d1nyra3 6.9 12 Fold:RRF/tRNA synthetase additional domain-like Superfamily:ThrRS/AlaRS common domain Family:Threonyl-tRNA synthetase (ThrRS), second 'additional' domain 21 c1xnwD_ not modelled 6.9 15 PDB header:ligase Chain: D: PDB Molecule:propionyl-coa carboxylase complex b subunit; PDBTitle: acyl-coa carboxylase beta subunit from s. coelicolor (pccb),2 apo form #2, mutant d422i 22 d1na6a2 not modelled 6.8 24 Fold:Restriction endonuclease-like Superfamily:Restriction endonuclease-like Family:Type II restriction endonuclease catalytic domain 23 c3rpjA_ not modelled 6.5 11 PDB header:transcription regulator Chain: A: PDB Molecule:curlin genes transcriptional regulator; PDBTitle: structure of a curlin genes transcriptional regulator protein from2 proteus mirabilis hi4320. 24 c1ij3A_ not modelled 6.3 29 PDB header:transcription Chain: A: PDB Molecule:general control protein gcn4; PDBTitle: gcn4-pvsl coiled-coil trimer with serine at the a(16)2 position 25 c1ws1A_ not modelled 6.1 17 PDB header:hydrolase Chain: A: PDB Molecule:peptide deformylase 1; PDBTitle: structure analysis of peptide deformylase from bacillus2 cereus 26 d1o4sa_ not modelled 6.1 17 Fold:PLP-dependent transferase-like Superfamily:PLP-dependent transferases Family:AAT-like 27 d1ix1a_ not modelled 6.1 17 Fold:Peptide deformylase Superfamily:Peptide deformylase Family:Peptide deformylase 28 d2glza1 not modelled 6.0 5 Fold:FwdE/GAPDH domain-like Superfamily:FwdE-like Family:FwdE-like 29 c3qu1B_ not modelled 6.0 17 PDB header:hydrolase, metal binding protein Chain: B: PDB Molecule:peptide deformylase 2; PDBTitle: peptide deformylase from vibrio cholerae 30 d1iwga1 not modelled 6.0 11 Fold:Ferredoxin-like Superfamily:Multidrug efflux transporter AcrB pore domain; PN1, PN2, PC1 and PC2 subdomains Family:Multidrug efflux transporter AcrB pore domain; PN1, PN2, PC1 and PC2 subdomains 31 c2w3tA_ not modelled 5.7 25 PDB header:hydrolase Chain: A: PDB Molecule:peptide deformylase; PDBTitle: chloro complex of the ni-form of e.coli deformylase 32 c2l06A_ not modelled 5.7 9 PDB header:protein binding Chain: A: PDB Molecule:phycobilisome lcm core-membrane linker polypeptide; PDBTitle: solution nmr structure of the pbs linker polypeptide domain (fragment2 254-400) of phycobilisome linker protein apce from synechocystis sp.3 pcc 6803. northeast structural genomics consortium target sgr209c 33 c2fyfB_ not modelled 5.6 11 PDB header:transferase Chain: B: PDB Molecule:phosphoserine aminotransferase; PDBTitle: structure of a putative phosphoserine aminotransferase from2 mycobacterium tuberculosis 34 d1pixa3 not modelled 5.5 10 Fold:ClpP/crotonase Superfamily:ClpP/crotonase Family:Biotin dependent carboxylase carboxyltransferase domain

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

Transmembrane helix prediction

Transmembrane helices have been predicted in your sequence to adopt the topology shown below

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0