Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	P	N	W	C	S	N	R	M	Y	F	S	G	E	P	A	Q	I	A	E	I	K	R	L	A	S	G	A	V	T	P	L	Y	R	R	A	T	N	E	G	I	Q	L	F	L	A	G	S	A	G	L	L	Q	T	T	E	D	V	R	F
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		E	P	C	P	G	L	T	A	A	G	R	G	V	V	S	P	E	N	I	A	F	T	R	W	L	T	H	L	Q	D	G	V	L	L	D	E	Q	N	C	L	M	L	H	E	L	W	L	Q	S	G	T	G	R	R	R	W	E	E	L	P
Secondary structure
SS confidence
Disorder									?	?	?	?	?	?	?	?	?	?																	?																	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.
Sequence		D	D	A	R	E	S	I	T	A	L	F	T	P	K	R	G	D	W	C	D	I	W	S	N	E	D	V	S	V	W	W	N	R	L	C	D	N	V	L	P	E	K	P	C	R	L	T	C
Secondary structure
SS confidence
Disorder																																					?					?	?	?	?	?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 2ijr chain A domain 1 Region: 3 - 166 Aligned: 164 Modelled: 163 Confidence: 100.0% Identity: 31% Fold: Api92-like Superfamily: Api92-like Family: Api92-like Phyre2 PDB 1xs8 chain A Region: 48 - 87 Aligned: 38 Modelled: 40 Confidence: 58.2% Identity: 21% Fold: YggX-like Superfamily: YggX-like Family: YggX-like Phyre2 PDB 1t07 chain A Region: 48 - 87 Aligned: 38 Modelled: 40 Confidence: 54.8% Identity: 21% Fold: YggX-like Superfamily: YggX-like Family: YggX-like Phyre2 PDB 1ec8 chain B Region: 91 - 118 Aligned: 26 Modelled: 28 Confidence: 37.6% Identity: 31% PDB header:lyase Chain: B: PDB Molecule:glucarate dehydratase; PDBTitle: e. coli glucarate dehydratase bound to product 2,3-2 dihydroxy-5-oxo-hexanedioate Phyre2 PDB 1tnd chain A Region: 12 - 111 Aligned: 90 Modelled: 90 Confidence: 26.8% Identity: 16% PDB header:binding protein(gtp) Chain: A: PDB Molecule:transducin; PDBTitle: the 2.2 angstroms crystal structure of transducin-alpha complexed with2 gtp gamma s Phyre2 PDB 2xtz chain B Region: 12 - 111 Aligned: 68 Modelled: 68 Confidence: 22.8% Identity: 18% PDB header:hydrolase Chain: B: PDB Molecule:guanine nucleotide-binding protein alpha-1 subunit; PDBTitle: crystal structure of the g alpha protein atgpa1 from2 arabidopsis thaliana Phyre2 PDB 1saz chain A domain 1 Region: 46 - 79 Aligned: 34 Modelled: 34 Confidence: 22.2% Identity: 15% Fold: Ribonuclease H-like motif Superfamily: Actin-like ATPase domain Family: Acetokinase-like Phyre2 PDB 2bj7 chain A domain 1 Region: 8 - 46 Aligned: 39 Modelled: 39 Confidence: 20.4% Identity: 13% Fold: Ribbon-helix-helix Superfamily: Ribbon-helix-helix Family: CopG-like Phyre2 PDB 2ca9 chain B Region: 8 - 52 Aligned: 45 Modelled: 45 Confidence: 20.4% Identity: 13% PDB header:transcriptional regulation Chain: B: PDB Molecule:putative nickel-responsive regulator; PDBTitle: apo-nikr from helicobacter pylori in closed trans-2 conformation Phyre2 PDB 1zcb chain A Region: 12 - 111 Aligned: 90 Modelled: 100 Confidence: 18.4% Identity: 14% PDB header:signaling protein Chain: A: PDB Molecule:g alpha i/13; PDBTitle: crystal structure of g alpha 13 in complex with gdp Phyre2 PDB 3n6j chain A Region: 91 - 121 Aligned: 29 Modelled: 31 Confidence: 16.8% Identity: 28% PDB header:isomerase Chain: A: PDB Molecule:mandelate racemase/muconate lactonizing protein; PDBTitle: crystal structure of mandelate racemase/muconate lactonizing protein2 from actinobacillus succinogenes 130z Phyre2 PDB 1tl7 chain C Region: 12 - 111 Aligned: 83 Modelled: 84 Confidence: 14.5% Identity: 23% PDB header:lyase Chain: C: PDB Molecule:guanine nucleotide-binding protein g(s), alpha PDBTitle: complex of gs- with the catalytic domains of mammalian2 adenylyl cyclase: complex with 2'(3')-o-(n-3 methylanthraniloyl)-guanosine 5'-triphosphate and mn Phyre2 PDB 3ijm chain A Region: 21 - 65 Aligned: 45 Modelled: 45 Confidence: 13.0% Identity: 18% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:uncharacterized restriction endonuclease-like fold PDBTitle: the structure of a restriction endonuclease-like fold superfamily2 protein from spirosoma linguale. Phyre2 PDB 2iho chain A domain 2 Region: 100 - 118 Aligned: 19 Modelled: 19 Confidence: 12.9% Identity: 26% Fold: Cysteine proteinases Superfamily: Cysteine proteinases Family: MOA C-terminal domain-like Phyre2 PDB 2a90 chain A domain 2 Region: 50 - 66 Aligned: 17 Modelled: 17 Confidence: 12.2% Identity: 18% Fold: WWE domain Superfamily: WWE domain Family: WWE domain Phyre2 PDB 1cip chain A Region: 12 - 111 Aligned: 90 Modelled: 90 Confidence: 12.2% Identity: 13% PDB header:hydrolase Chain: A: PDB Molecule:protein (guanine nucleotide-binding protein PDBTitle: gi-alpha-1 subunit of guanine nucleotide-binding protein2 complexed with a gtp analogue Phyre2 PDB 3ue9 chain A Region: 106 - 130 Aligned: 25 Modelled: 25 Confidence: 12.1% Identity: 36% PDB header:ligase Chain: A: PDB Molecule:adenylosuccinate synthetase; PDBTitle: crystal structure of adenylosuccinate synthetase (ampsase) (pura) from2 burkholderia thailandensis Phyre2 PDB 1osc chain E Region: 62 - 86 Aligned: 22 Modelled: 25 Confidence: 11.8% Identity: 23% Fold: Ferredoxin-like Superfamily: GlnB-like Family: Divalent ion tolerance proteins CutA (CutA1) Phyre2 PDB 1iwe chain A Region: 106 - 130 Aligned: 25 Modelled: 25 Confidence: 11.6% Identity: 36% Fold: P-loop containing nucleoside triphosphate hydrolases Superfamily: P-loop containing nucleoside triphosphate hydrolases Family: Nitrogenase iron protein-like Phyre2 PDB 1iwe chain B Region: 106 - 130 Aligned: 25 Modelled: 25 Confidence: 11.4% Identity: 36% PDB header:ligase Chain: B: PDB Molecule:adenylosuccinate synthetase; PDBTitle: imp complex of the recombinant mouse-muscle2 adenylosuccinate synthetase Phyre2 1 d2ijra1 2 d1xs8a_ 3 d1t07a_ 4 c1ec8B_ 5 c1tndA_ 6 c2xtzB_ 7 d1saza1 8 d2bj7a1 9 c2ca9B_ 10 c1zcbA_ 11 c3n6jA_ 12 c1tl7C_ 13 c3ijmA_ 14 d2ihoa2 15 d2a90a2 16 c1cipA_ 17 c3ue9A_ 18 d1osce_ 19 d1iwea_ 20 c1iweB_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 d2ijra1 100.0 31 Fold:Api92-like Superfamily:Api92-like Family:Api92-like 2 d1xs8a_ 58.2 21 Fold:YggX-like Superfamily:YggX-like Family:YggX-like 3 d1t07a_ 54.8 21 Fold:YggX-like Superfamily:YggX-like Family:YggX-like 4 c1ec8B_ 37.6 31 PDB header:lyase Chain: B: PDB Molecule:glucarate dehydratase; PDBTitle: e. coli glucarate dehydratase bound to product 2,3-2 dihydroxy-5-oxo-hexanedioate 5 c1tndA_ 26.8 16 PDB header:binding protein(gtp) Chain: A: PDB Molecule:transducin; PDBTitle: the 2.2 angstroms crystal structure of transducin-alpha complexed with2 gtp gamma s 6 c2xtzB_ 22.8 18 PDB header:hydrolase Chain: B: PDB Molecule:guanine nucleotide-binding protein alpha-1 subunit; PDBTitle: crystal structure of the g alpha protein atgpa1 from2 arabidopsis thaliana 7 d1saza1 22.2 15 Fold:Ribonuclease H-like motif Superfamily:Actin-like ATPase domain Family:Acetokinase-like 8 d2bj7a1 20.4 13 Fold:Ribbon-helix-helix Superfamily:Ribbon-helix-helix Family:CopG-like 9 c2ca9B_ 20.4 13 PDB header:transcriptional regulation Chain: B: PDB Molecule:putative nickel-responsive regulator; PDBTitle: apo-nikr from helicobacter pylori in closed trans-2 conformation 10 c1zcbA_ 18.4 14 PDB header:signaling protein Chain: A: PDB Molecule:g alpha i/13; PDBTitle: crystal structure of g alpha 13 in complex with gdp 11 c3n6jA_ 16.8 28 PDB header:isomerase Chain: A: PDB Molecule:mandelate racemase/muconate lactonizing protein; PDBTitle: crystal structure of mandelate racemase/muconate lactonizing protein2 from actinobacillus succinogenes 130z 12 c1tl7C_ 14.5 23 PDB header:lyase Chain: C: PDB Molecule:guanine nucleotide-binding protein g(s), alpha PDBTitle: complex of gs- with the catalytic domains of mammalian2 adenylyl cyclase: complex with 2'(3')-o-(n-3 methylanthraniloyl)-guanosine 5'-triphosphate and mn 13 c3ijmA_ 13.0 18 PDB header:structural genomics, unknown function Chain: A: PDB Molecule:uncharacterized restriction endonuclease-like fold PDBTitle: the structure of a restriction endonuclease-like fold superfamily2 protein from spirosoma linguale. 14 d2ihoa2 12.9 26 Fold:Cysteine proteinases Superfamily:Cysteine proteinases Family:MOA C-terminal domain-like 15 d2a90a2 12.2 18 Fold:WWE domain Superfamily:WWE domain Family:WWE domain 16 c1cipA_ 12.2 13 PDB header:hydrolase Chain: A: PDB Molecule:protein (guanine nucleotide-binding protein PDBTitle: gi-alpha-1 subunit of guanine nucleotide-binding protein2 complexed with a gtp analogue 17 c3ue9A_ 12.1 36 PDB header:ligase Chain: A: PDB Molecule:adenylosuccinate synthetase; PDBTitle: crystal structure of adenylosuccinate synthetase (ampsase) (pura) from2 burkholderia thailandensis 18 d1osce_ 11.8 23 Fold:Ferredoxin-like Superfamily:GlnB-like Family:Divalent ion tolerance proteins CutA (CutA1) 19 d1iwea_ 11.6 36 Fold:P-loop containing nucleoside triphosphate hydrolases Superfamily:P-loop containing nucleoside triphosphate hydrolases Family:Nitrogenase iron protein-like 20 c1iweB_ 11.4 36 PDB header:ligase Chain: B: PDB Molecule:adenylosuccinate synthetase; PDBTitle: imp complex of the recombinant mouse-muscle2 adenylosuccinate synthetase 21 d1ngka_ not modelled 10.6 16 Fold:Globin-like Superfamily:Globin-like Family:Truncated hemoglobin 22 c3cx6A_ not modelled 10.3 13 PDB header:signaling protein Chain: A: PDB Molecule:guanine nucleotide-binding protein alpha-13 PDBTitle: crystal structure of pdzrhogef rgrgs domain in a complex2 with galpha-13 bound to gdp 23 d2ozka1 not modelled 10.0 56 Fold:S-adenosyl-L-methionine-dependent methyltransferases Superfamily:S-adenosyl-L-methionine-dependent methyltransferases Family:Nsp15 N-terminal domain-like 24 c2bcjQ_ not modelled 9.9 12 PDB header:transferase/hydrolase Chain: Q: PDB Molecule:g alpha i1, guanine nucleotide-binding protein g(q), alpha PDBTitle: crystal structure of g protein-coupled receptor kinase 2 in complex2 with galpha-q and gbetagamma subunits 25 d2guka1 not modelled 9.5 27 Fold:PG1857-like Superfamily:PG1857-like Family:PG1857-like 26 c3iuzA_ not modelled 9.1 15 PDB header:lyase Chain: A: PDB Molecule:putative glyoxalase superfamily protein; PDBTitle: crystal structure of putative glyoxalase superfamily protein2 (yp_299723.1) from ralstonia eutropha jmp134 at 1.90 a resolution 27 d2gqfa2 not modelled 8.9 3 Fold:HI0933 insert domain-like Superfamily:HI0933 insert domain-like Family:HI0933 insert domain-like 28 d1jdfa1 not modelled 8.9 38 Fold:TIM beta/alpha-barrel Superfamily:Enolase C-terminal domain-like Family:D-glucarate dehydratase-like 29 d2h85a1 not modelled 8.6 56 Fold:S-adenosyl-L-methionine-dependent methyltransferases Superfamily:S-adenosyl-L-methionine-dependent methyltransferases Family:Nsp15 N-terminal domain-like 30 c1sxjB_ not modelled 8.1 22 PDB header:replication Chain: B: PDB Molecule:activator 1 37 kda subunit; PDBTitle: crystal structure of the eukaryotic clamp loader2 (replication factor c, rfc) bound to the dna sliding clamp3 (proliferating cell nuclear antigen, pcna) 31 d1p9ba_ not modelled 8.1 28 Fold:P-loop containing nucleoside triphosphate hydrolases Superfamily:P-loop containing nucleoside triphosphate hydrolases Family:Nitrogenase iron protein-like 32 d1ac5a_ not modelled 8.0 13 Fold:alpha/beta-Hydrolases Superfamily:alpha/beta-Hydrolases Family:Serine carboxypeptidase-like 33 d1sxjc2 not modelled 7.9 19 Fold:P-loop containing nucleoside triphosphate hydrolases Superfamily:P-loop containing nucleoside triphosphate hydrolases Family:Extended AAA-ATPase domain 34 c3thgA_ not modelled 7.8 14 PDB header:protein binding Chain: A: PDB Molecule:ribulose bisphosphate carboxylase/oxygenase activase 1, PDBTitle: crystal structure of the creosote rubisco activase c-domain 35 d2bcjq1 not modelled 7.6 11 Fold:Transducin (alpha subunit), insertion domain Superfamily:Transducin (alpha subunit), insertion domain Family:Transducin (alpha subunit), insertion domain 36 c2d7uA_ not modelled 7.6 24 PDB header:ligase Chain: A: PDB Molecule:adenylosuccinate synthetase; PDBTitle: crystal structure of hypothetical adenylosuccinate synthetase, ph04382 from pyrococcus horikoshii ot3 37 c1iqpF_ not modelled 7.5 25 PDB header:replication Chain: F: PDB Molecule:rfcs; PDBTitle: crystal structure of the clamp loader small subunit from2 pyrococcus furiosus 38 c3gnzP_ not modelled 7.3 25 PDB header:toxin Chain: P: PDB Molecule:25 kda protein elicitor; PDBTitle: toxin fold for microbial attack and plant defense 39 c2zzxD_ not modelled 7.3 32 PDB header:transport protein Chain: D: PDB Molecule:abc transporter, solute-binding protein; PDBTitle: crystal structure of a periplasmic substrate binding protein in2 complex with lactate 40 c1rrzA_ not modelled 7.2 33 PDB header:structural genomics,biosynthetic protein Chain: A: PDB Molecule:glycogen synthesis protein glgs; PDBTitle: solution structure of glgs protein from e. coli 41 d1rrza_ not modelled 7.2 33 Fold:Spectrin repeat-like Superfamily:Glycogen synthesis protein GlgS Family:Glycogen synthesis protein GlgS 42 c2xykB_ not modelled 7.0 13 PDB header:oxygen storage/transport Chain: B: PDB Molecule:2-on-2 hemoglobin; PDBTitle: group ii 2-on-2 hemoglobin from the plant pathogen2 agrobacterium tumefaciens 43 d2ixma1 not modelled 7.0 19 Fold:PTPA-like Superfamily:PTPA-like Family:PTPA-like 44 d1zcaa1 not modelled 6.8 23 Fold:Transducin (alpha subunit), insertion domain Superfamily:Transducin (alpha subunit), insertion domain Family:Transducin (alpha subunit), insertion domain 45 d1dj2a_ not modelled 6.6 20 Fold:P-loop containing nucleoside triphosphate hydrolases Superfamily:P-loop containing nucleoside triphosphate hydrolases Family:Nitrogenase iron protein-like 46 d2hzaa1 not modelled 6.5 13 Fold:Ribbon-helix-helix Superfamily:Ribbon-helix-helix Family:CopG-like 47 d1nmla2 not modelled 5.8 22 Fold:Cytochrome c Superfamily:Cytochrome c Family:Di-heme cytochrome c peroxidase 48 d2ixoa1 not modelled 5.8 25 Fold:PTPA-like Superfamily:PTPA-like Family:PTPA-like 49 c2bj3D_ not modelled 5.5 13 PDB header:transcription Chain: D: PDB Molecule:nickel responsive regulator; PDBTitle: nikr-apo 50 c2chgB_ not modelled 5.5 22 PDB header:dna-binding protein Chain: B: PDB Molecule:replication factor c small subunit; PDBTitle: replication factor c domains 1 and 2 51 d1pyla_ not modelled 5.3 12 Fold:Microbial ribonucleases Superfamily:Microbial ribonucleases Family:Bacterial ribonucleases 52 c2a3zC_ not modelled 5.2 57 PDB header:structural protein Chain: C: PDB Molecule:wiskott-aldrich syndrome protein; PDBTitle: ternary complex of the wh2 domain of wasp with actin-dnase i 53 c3d33B_ not modelled 5.2 33 PDB header:unknown function Chain: B: PDB Molecule:domain of unknown function with an immunoglobulin-like PDBTitle: crystal structure of a duf3244 family protein with an immunoglobulin-2 like beta-sandwich fold (bvu_0276) from bacteroides vulgatus atcc3 8482 at 1.70 a resolution 54 c2g62A_ not modelled 5.2 25 PDB header:hydrolase activator Chain: A: PDB Molecule:protein phosphatase 2a, regulatory subunit b' (pr 53); PDBTitle: crystal structure of human ptpa 55 c2rhbD_ not modelled 5.1 56 PDB header:viral protein Chain: D: PDB Molecule:uridylate-specific endoribonuclease; PDBTitle: crystal structure of nsp15-h234a mutant- hexamer in2 asymmetric unit 56 d1sxjb2 not modelled 5.1 22 Fold:P-loop containing nucleoside triphosphate hydrolases Superfamily:P-loop containing nucleoside triphosphate hydrolases Family:Extended AAA-ATPase domain 57 c2nz7A_ not modelled 5.1 21 PDB header:apoptosis Chain: A: PDB Molecule:caspase recruitment domain-containing protein 4; PDBTitle: crystal structure analysis of caspase-recruitment domain2 (card) of nod1 58 c1z7s1_ not modelled 5.0 38 PDB header:virus Chain: 1: PDB Molecule:human coxsackievirus a21; PDB Fragment:viral protein 1; PDBTitle: the crystal structure of coxsackievirus a21

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0