Email l.a.kelley@imperial.ac.uk Description A6X972 Date Sun Jul 8 11:45:02 BST 2012 Unique Job ID c0cbb688e9c4da63

Summary

Top model

Image coloured by rainbow N → C terminus Close Jmol

Model (left) based on template d2okua1 Top template information Fold:N-cbl like Superfamily:PG0775 C-terminal domain-like Family:PG0775 C-terminal domain-like Confidence and coverage Confidence: 27.2% Coverage: 52% 38 residues ( 52% of your sequence) have been modelled with 27.2% confidence by the single highest scoring template. You may wish to submit your sequence to Phyrealarm. This will automatically scan your sequence every week for new potential templates as they appear in the Phyre2 library. Please note: You must be registered and logged in to use Phyrealarm. 3D viewing Interactive 3D view in Jmol

Sequence analysis

Secondary structure and disorder prediction

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 2oku chain A domain 1 Region: 14 - 51 Aligned: 38 Modelled: 38 Confidence: 27.2% Identity: 18% Fold: N-cbl like Superfamily: PG0775 C-terminal domain-like Family: PG0775 C-terminal domain-like Phyre2 PDB 1dlc chain A domain 3 Region: 25 - 59 Aligned: 35 Modelled: 35 Confidence: 24.4% Identity: 17% Fold: Toxins' membrane translocation domains Superfamily: delta-Endotoxin (insectocide), N-terminal domain Family: delta-Endotoxin (insectocide), N-terminal domain Phyre2 PDB 2es9 chain A domain 1 Region: 43 - 57 Aligned: 15 Modelled: 15 Confidence: 23.1% Identity: 53% Fold: YoaC-like Superfamily: YoaC-like Family: YoaC-like Phyre2 PDB 1ciy chain A domain 3 Region: 25 - 58 Aligned: 34 Modelled: 34 Confidence: 19.0% Identity: 15% Fold: Toxins' membrane translocation domains Superfamily: delta-Endotoxin (insectocide), N-terminal domain Family: delta-Endotoxin (insectocide), N-terminal domain Phyre2 PDB 1n1b chain A domain 1 Region: 18 - 55 Aligned: 38 Modelled: 38 Confidence: 18.4% Identity: 21% Fold: alpha/alpha toroid Superfamily: Terpenoid cyclases/Protein prenyltransferases Family: Terpenoid cyclase N-terminal domain Phyre2 PDB 1svf chain C Region: 37 - 50 Aligned: 14 Modelled: 14 Confidence: 16.5% Identity: 57% PDB header:viral protein Chain: C: PDB Molecule:protein (fusion glycoprotein); PDBTitle: paramyxovirus sv5 fusion protein core Phyre2 PDB 1ji6 chain A domain 3 Region: 25 - 59 Aligned: 35 Modelled: 35 Confidence: 15.9% Identity: 17% Fold: Toxins' membrane translocation domains Superfamily: delta-Endotoxin (insectocide), N-terminal domain Family: delta-Endotoxin (insectocide), N-terminal domain Phyre2 PDB 1i5p chain A domain 3 Region: 25 - 63 Aligned: 39 Modelled: 39 Confidence: 14.1% Identity: 13% Fold: Toxins' membrane translocation domains Superfamily: delta-Endotoxin (insectocide), N-terminal domain Family: delta-Endotoxin (insectocide), N-terminal domain Phyre2 PDB 2wuk chain D Region: 25 - 47 Aligned: 23 Modelled: 23 Confidence: 11.9% Identity: 13% PDB header:cell cycle Chain: D: PDB Molecule:septum site-determining protein diviva; PDBTitle: diviva n-terminal domain, f17a mutant Phyre2 PDB 3tu3 chain B Region: 21 - 37 Aligned: 17 Modelled: 17 Confidence: 10.6% Identity: 24% PDB header:toxin/toxin chaperone Chain: B: PDB Molecule:exou; PDBTitle: 1.92 angstrom resolution crystal structure of the full-length spcu in2 complex with full-length exou from the type iii secretion system of3 pseudomonas aeruginosa Phyre2 PDB 2yx8 chain A Region: 44 - 64 Aligned: 21 Modelled: 21 Confidence: 9.5% Identity: 14% PDB header:protein transport Chain: A: PDB Molecule:receptor activity-modifying protein 1; PDBTitle: crystal structure of the extracellular domain of human ramp1 Phyre2 PDB 1n20 chain A Region: 18 - 55 Aligned: 38 Modelled: 38 Confidence: 9.5% Identity: 21% PDB header:isomerase Chain: A: PDB Molecule:(+)-bornyl diphosphate synthase; PDBTitle: (+)-bornyl diphosphate synthase: complex with mg and 3-aza-2 2,3-dihydrogeranyl diphosphate Phyre2 PDB 2fyz chain C Region: 30 - 50 Aligned: 21 Modelled: 21 Confidence: 8.1% Identity: 43% PDB header:protein binding Chain: C: PDB Molecule:fusion glycoprotein f0; PDBTitle: structural of mumps virus fusion protein core Phyre2 PDB 1x4t chain A domain 1 Region: 41 - 60 Aligned: 20 Modelled: 20 Confidence: 7.4% Identity: 25% Fold: Long alpha-hairpin Superfamily: ISY1 domain-like Family: ISY1 N-terminal domain-like Phyre2 PDB 1dlc chain A Region: 25 - 58 Aligned: 34 Modelled: 34 Confidence: 6.5% Identity: 18% PDB header:toxin Chain: A: PDB Molecule:delta-endotoxin cryiiia; PDBTitle: crystal structure of insecticidal delta-endotoxin from2 bacillus thuringiensis at 2.5 angstroms resolution Phyre2 PDB 3eb7 chain B Region: 25 - 58 Aligned: 34 Modelled: 34 Confidence: 6.3% Identity: 21% PDB header:toxin Chain: B: PDB Molecule:insecticidal delta-endotoxin cry8ea1; PDBTitle: crystal structure of insecticidal delta-endotoxin cry8ea1 from2 bacillus thuringiensis at 2.2 angstroms resolution Phyre2 PDB 2roc chain B Region: 38 - 57 Aligned: 20 Modelled: 20 Confidence: 6.0% Identity: 25% PDB header:apoptosis Chain: B: PDB Molecule:bcl-2-binding component 3; PDBTitle: solution structure of mcl-1 complexed with puma Phyre2 PDB 1ljm chain A Region: 20 - 33 Aligned: 14 Modelled: 14 Confidence: 5.5% Identity: 43% Fold: Common fold of diphtheria toxin/transcription factors/cytochrome f Superfamily: p53-like transcription factors Family: RUNT domain Phyre2 PDB 1y71 chain A domain 1 Region: 26 - 52 Aligned: 26 Modelled: 27 Confidence: 5.5% Identity: 31% Fold: SH3-like barrel Superfamily: Kinase-associated protein B-like Family: Kinase-associated protein B-like Phyre2 PDB 2w56 chain B Region: 25 - 31 Aligned: 7 Modelled: 7 Confidence: 5.4% Identity: 71% PDB header:unknown function Chain: B: PDB Molecule:vc0508; PDBTitle: structure of the hypothetical protein vc0508 from vibrio cholerae2 vsp-ii pathogenicity island Phyre2 1 d2okua1 2 d1dlca3 3 d2es9a1 4 d1ciya3 5 d1n1ba1 6 c1svfC_ 7 d1ji6a3 8 d1i5pa3 9 c2wukD_ 10 c3tu3B_ 11 c2yx8A_ 12 c1n20A_ 13 c2fyzC_ 14 d1x4ta1 15 c1dlcA_ 16 c3eb7B_ 17 c2rocB_ 18 d1ljma_ 19 d1y71a1 20 c2w56B_ 21

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0