Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	Y	Q	D	K	I	L	V	R	Q	L	G	L	Q	P	Y	E	P	I	S	Q	A	M	H	E	F	T	D	T	R	D	D	S	T	L	D	E	I	W	L	V	E	H	Y	P	V	F	T	Q	G	Q	A	G	K	A	E	H	I	L	M
Secondary structure
SS confidence
Disorder		?	?	?	?	?																									?	?																									?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		P	G	D	I	P	V	I	Q	S	D	R	G	G	Q	V	T	Y	H	G	P	G	Q	Q	V	M	Y	V	L	L	N	L	K	R	R	K	L	G	V	R	E	L	V	T	L	L	E	Q	T	V	V	N	T	L	A	E	L	G	I	E	A
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		H	P	R	A	D	A	P	G	V	Y	V	G	E	K	K	I	C	S	L	G	L	R	I	R	R	G	C	S	F	H	G	L	A	L	N	V	N	M	D	L	S	P	F	L	R	I	N	P	C	G	Y	A	G	M	E	M	A	K	I	S
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.
Sequence		Q	W	K	P	E	A	T	T	N	N	I	A	P	R	L	L	E	N	I	L	A	L	L	N	N	P	D	F	E	Y	I	T	A
Secondary structure
SS confidence
Disorder																															?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 1w66 chain A domain 1 Region: 2 - 206 Aligned: 203 Modelled: 205 Confidence: 100.0% Identity: 31% Fold: Class II aaRS and biotin synthetases Superfamily: Class II aaRS and biotin synthetases Family: LplA-like Phyre2 PDB 2qhv chain A Region: 5 - 208 Aligned: 201 Modelled: 204 Confidence: 100.0% Identity: 32% PDB header:transferase Chain: A: PDB Molecule:lipoyltransferase; PDBTitle: structural basis of octanoic acid recognition by lipoate-protein2 ligase b Phyre2 PDB 2qhs chain A Region: 5 - 208 Aligned: 201 Modelled: 204 Confidence: 100.0% Identity: 32% PDB header:transferase Chain: A: PDB Molecule:lipoyltransferase; PDBTitle: structural basis of octanoic acid recognition by lipoate-protein2 ligase b Phyre2 PDB 1x2g chain B Region: 4 - 206 Aligned: 195 Modelled: 197 Confidence: 100.0% Identity: 16% PDB header:ligase Chain: B: PDB Molecule:lipoate-protein ligase a; PDBTitle: crystal structure of lipate-protein ligase a from2 escherichia coli Phyre2 PDB 1vqz chain A Region: 6 - 212 Aligned: 202 Modelled: 206 Confidence: 100.0% Identity: 17% PDB header:ligase Chain: A: PDB Molecule:lipoate-protein ligase, putative; PDBTitle: crystal structure of a putative lipoate-protein ligase a (sp_1160)2 from streptococcus pneumoniae tigr4 at 1.99 a resolution Phyre2 PDB 2e5a chain A Region: 7 - 211 Aligned: 200 Modelled: 205 Confidence: 100.0% Identity: 15% PDB header:ligase Chain: A: PDB Molecule:lipoyltransferase 1; PDBTitle: crystal structure of bovine lipoyltransferase in complex2 with lipoyl-amp Phyre2 PDB 1x2g chain A domain 2 Region: 4 - 213 Aligned: 202 Modelled: 204 Confidence: 100.0% Identity: 17% Fold: Class II aaRS and biotin synthetases Superfamily: Class II aaRS and biotin synthetases Family: LplA-like Phyre2 PDB 2c8m chain A domain 1 Region: 4 - 206 Aligned: 195 Modelled: 203 Confidence: 100.0% Identity: 18% Fold: Class II aaRS and biotin synthetases Superfamily: Class II aaRS and biotin synthetases Family: LplA-like Phyre2 PDB 1vqz chain A domain 2 Region: 7 - 212 Aligned: 201 Modelled: 203 Confidence: 100.0% Identity: 17% Fold: Class II aaRS and biotin synthetases Superfamily: Class II aaRS and biotin synthetases Family: LplA-like Phyre2 PDB 2p5i chain A domain 1 Region: 7 - 212 Aligned: 205 Modelled: 206 Confidence: 100.0% Identity: 13% Fold: Class II aaRS and biotin synthetases Superfamily: Class II aaRS and biotin synthetases Family: LplA-like Phyre2 PDB 2p0l chain A domain 1 Region: 3 - 207 Aligned: 203 Modelled: 205 Confidence: 100.0% Identity: 11% Fold: Class II aaRS and biotin synthetases Superfamily: Class II aaRS and biotin synthetases Family: LplA-like Phyre2 PDB 2zgw chain A domain 2 Region: 69 - 203 Aligned: 122 Modelled: 135 Confidence: 99.2% Identity: 20% Fold: Class II aaRS and biotin synthetases Superfamily: Class II aaRS and biotin synthetases Family: Biotin holoenzyme synthetase Phyre2 PDB 2ewn chain A Region: 69 - 203 Aligned: 129 Modelled: 135 Confidence: 99.0% Identity: 15% PDB header:ligase, transcription Chain: A: PDB Molecule:bira bifunctional protein; PDBTitle: ecoli biotin repressor with co-repressor analog Phyre2 PDB 2ej9 chain A Region: 69 - 203 Aligned: 123 Modelled: 135 Confidence: 99.0% Identity: 18% PDB header:ligase Chain: A: PDB Molecule:putative biotin ligase; PDBTitle: crystal structure of biotin protein ligase from2 methanococcus jannaschii Phyre2 PDB 2eay chain B Region: 66 - 203 Aligned: 122 Modelled: 132 Confidence: 98.9% Identity: 21% PDB header:ligase Chain: B: PDB Molecule:biotin [acetyl-coa-carboxylase] ligase; PDBTitle: crystal structure of biotin protein ligase from aquifex2 aeolicus Phyre2 PDB 3bfm chain A Region: 69 - 203 Aligned: 125 Modelled: 135 Confidence: 98.8% Identity: 11% PDB header:unknown function Chain: A: PDB Molecule:biotin protein ligase-like protein of unknown function; PDBTitle: crystal structure of a biotin protein ligase-like protein of unknown2 function (tm1040_0394) from silicibacter sp. tm1040 at 1.70 a3 resolution Phyre2 PDB 2dzc chain A Region: 66 - 203 Aligned: 118 Modelled: 138 Confidence: 98.8% Identity: 23% PDB header:ligase Chain: A: PDB Molecule:biotin--[acetyl-coa-carboxylase] ligase; PDBTitle: crystal structure of biotin protein ligase from pyrococcus2 horikoshii, mutation r48a Phyre2 PDB 1bia chain A domain 3 Region: 44 - 203 Aligned: 122 Modelled: 141 Confidence: 98.6% Identity: 16% Fold: Class II aaRS and biotin synthetases Superfamily: Class II aaRS and biotin synthetases Family: Biotin holoenzyme synthetase Phyre2 PDB 2cgh chain B Region: 81 - 203 Aligned: 107 Modelled: 109 Confidence: 98.6% Identity: 19% PDB header:ligase Chain: B: PDB Molecule:biotin ligase; PDBTitle: crystal structure of biotin ligase from mycobacterium2 tuberculosis Phyre2 PDB 2ddz chain A domain 1 Region: 103 - 204 Aligned: 88 Modelled: 102 Confidence: 97.5% Identity: 20% Fold: Class II aaRS and biotin synthetases Superfamily: Class II aaRS and biotin synthetases Family: PH0223-like Phyre2 1 d1w66a1 2 c2qhvA_ 3 c2qhsA_ 4 c1x2gB_ 5 c1vqzA_ 6 c2e5aA_ 7 d1x2ga2 8 d2c8ma1 9 d1vqza2 10 d2p5ia1 11 d2p0la1 12 d2zgwa2 13 c2ewnA_ 14 c2ej9A_ 15 c2eayB_ 16 c3bfmA_ 17 c2dzcA_ 18 d1biaa3 19 c2cghB_ 20 d2ddza1 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 d1w66a1 100.0 31 Fold:Class II aaRS and biotin synthetases Superfamily:Class II aaRS and biotin synthetases Family:LplA-like 2 c2qhvA_ 100.0 32 PDB header:transferase Chain: A: PDB Molecule:lipoyltransferase; PDBTitle: structural basis of octanoic acid recognition by lipoate-protein2 ligase b 3 c2qhsA_ 100.0 32 PDB header:transferase Chain: A: PDB Molecule:lipoyltransferase; PDBTitle: structural basis of octanoic acid recognition by lipoate-protein2 ligase b 4 c1x2gB_ 100.0 16 PDB header:ligase Chain: B: PDB Molecule:lipoate-protein ligase a; PDBTitle: crystal structure of lipate-protein ligase a from2 escherichia coli 5 c1vqzA_ 100.0 17 PDB header:ligase Chain: A: PDB Molecule:lipoate-protein ligase, putative; PDBTitle: crystal structure of a putative lipoate-protein ligase a (sp_1160)2 from streptococcus pneumoniae tigr4 at 1.99 a resolution 6 c2e5aA_ 100.0 15 PDB header:ligase Chain: A: PDB Molecule:lipoyltransferase 1; PDBTitle: crystal structure of bovine lipoyltransferase in complex2 with lipoyl-amp 7 d1x2ga2 100.0 17 Fold:Class II aaRS and biotin synthetases Superfamily:Class II aaRS and biotin synthetases Family:LplA-like 8 d2c8ma1 100.0 18 Fold:Class II aaRS and biotin synthetases Superfamily:Class II aaRS and biotin synthetases Family:LplA-like 9 d1vqza2 100.0 17 Fold:Class II aaRS and biotin synthetases Superfamily:Class II aaRS and biotin synthetases Family:LplA-like 10 d2p5ia1 100.0 13 Fold:Class II aaRS and biotin synthetases Superfamily:Class II aaRS and biotin synthetases Family:LplA-like 11 d2p0la1 100.0 11 Fold:Class II aaRS and biotin synthetases Superfamily:Class II aaRS and biotin synthetases Family:LplA-like 12 d2zgwa2 99.2 20 Fold:Class II aaRS and biotin synthetases Superfamily:Class II aaRS and biotin synthetases Family:Biotin holoenzyme synthetase 13 c2ewnA_ 99.0 15 PDB header:ligase, transcription Chain: A: PDB Molecule:bira bifunctional protein; PDBTitle: ecoli biotin repressor with co-repressor analog 14 c2ej9A_ 99.0 18 PDB header:ligase Chain: A: PDB Molecule:putative biotin ligase; PDBTitle: crystal structure of biotin protein ligase from2 methanococcus jannaschii 15 c2eayB_ 98.9 21 PDB header:ligase Chain: B: PDB Molecule:biotin [acetyl-coa-carboxylase] ligase; PDBTitle: crystal structure of biotin protein ligase from aquifex2 aeolicus 16 c3bfmA_ 98.8 11 PDB header:unknown function Chain: A: PDB Molecule:biotin protein ligase-like protein of unknown function; PDBTitle: crystal structure of a biotin protein ligase-like protein of unknown2 function (tm1040_0394) from silicibacter sp. tm1040 at 1.70 a3 resolution 17 c2dzcA_ 98.8 23 PDB header:ligase Chain: A: PDB Molecule:biotin--[acetyl-coa-carboxylase] ligase; PDBTitle: crystal structure of biotin protein ligase from pyrococcus2 horikoshii, mutation r48a 18 d1biaa3 98.6 16 Fold:Class II aaRS and biotin synthetases Superfamily:Class II aaRS and biotin synthetases Family:Biotin holoenzyme synthetase 19 c2cghB_ 98.6 19 PDB header:ligase Chain: B: PDB Molecule:biotin ligase; PDBTitle: crystal structure of biotin ligase from mycobacterium2 tuberculosis 20 d2ddza1 97.5 20 Fold:Class II aaRS and biotin synthetases Superfamily:Class II aaRS and biotin synthetases Family:PH0223-like 21 d2qfra2 not modelled 22.8 16 Fold:Metallo-dependent phosphatases Superfamily:Metallo-dependent phosphatases Family:Purple acid phosphatase-like 22 c3lr4A_ not modelled 19.7 19 PDB header:transferase Chain: A: PDB Molecule:sensor protein; PDBTitle: periplasmic domain of the riss sensor protein from burkholderia2 pseudomallei, barium phased at low ph 23 d1knxa1 not modelled 17.9 11 Fold:MurF and HprK N-domain-like Superfamily:HprK N-terminal domain-like Family:HPr kinase/phoshatase HprK N-terminal domain 24 c1xzwB_ not modelled 12.1 16 PDB header:hydrolase Chain: B: PDB Molecule:purple acid phosphatase; PDBTitle: sweet potato purple acid phosphatase/phosphate complex 25 c1kbpB_ not modelled 11.9 16 PDB header:hydrolase (phosphoric monoester) Chain: B: PDB Molecule:purple acid phosphatase; PDBTitle: kidney bean purple acid phosphatase 26 c3uotB_ not modelled 10.1 11 PDB header:cell cycle Chain: B: PDB Molecule:mediator of dna damage checkpoint protein 1; PDBTitle: crystal structure of mdc1 fha domain in complex with a phosphorylated2 peptide from the mdc1 n-terminus 27 d1ra0a1 not modelled 9.8 17 Fold:Composite domain of metallo-dependent hydrolases Superfamily:Composite domain of metallo-dependent hydrolases Family:Cytosine deaminase 28 c1ponB_ not modelled 8.2 15 PDB header:calcium-binding protein Chain: B: PDB Molecule:troponin c; PDBTitle: site iii-site iv troponin c heterodimer, nmr 29 c2kd2A_ not modelled 7.3 22 PDB header:apoptosis Chain: A: PDB Molecule:fas apoptotic inhibitory molecule 1; PDBTitle: nmr structure of faim-ctd 30 d1m7ja1 not modelled 7.2 31 Fold:Composite domain of metallo-dependent hydrolases Superfamily:Composite domain of metallo-dependent hydrolases Family:D-aminoacylase 31 d1vsra_ not modelled 7.2 23 Fold:Restriction endonuclease-like Superfamily:Restriction endonuclease-like Family:Very short patch repair (VSR) endonuclease 32 c2j3tB_ not modelled 6.9 9 PDB header:transport Chain: B: PDB Molecule:trafficking protein particle complex subunit 6a; PDBTitle: the crystal structure of the bet3-trs33-bet5-trs23 complex. 33 d1qbaa1 not modelled 6.6 22 Fold:Immunoglobulin-like beta-sandwich Superfamily:E set domains Family:E-set domains of sugar-utilizing enzymes 34 c2faoB_ not modelled 6.5 20 PDB header:hydrolase/transferase Chain: B: PDB Molecule:probable atp-dependent dna ligase; PDBTitle: crystal structure of pseudomonas aeruginosa ligd polymerase2 domain 35 c2iruA_ not modelled 6.2 16 PDB header:transferase Chain: A: PDB Molecule:putative dna ligase-like protein rv0938/mt0965; PDBTitle: crystal structure of the polymerase domain from mycobacterium2 tuberculosis ligase d 36 d1xzwa2 not modelled 5.6 16 Fold:Metallo-dependent phosphatases Superfamily:Metallo-dependent phosphatases Family:Purple acid phosphatase-like 37 d1kfia3 not modelled 5.5 11 Fold:Phosphoglucomutase, first 3 domains Superfamily:Phosphoglucomutase, first 3 domains Family:Phosphoglucomutase, first 3 domains 38 c2jpeA_ not modelled 5.4 9 PDB header:transcription Chain: A: PDB Molecule:nuclear inhibitor of protein phosphatase 1; PDBTitle: fha domain of nipp1 39 d1k1da1 not modelled 5.4 23 Fold:Composite domain of metallo-dependent hydrolases Superfamily:Composite domain of metallo-dependent hydrolases Family:Hydantoinase (dihydropyrimidinase)

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0