Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		R	A	L	F	T	W	K	V	L	L	G	A	A	V	D	A	L	F	A	N	D	G	A	A	L	I	L	T	P	I	V	I	A	M	L	I	A	L	G	F	S	Q	G	T	T	L	A	F	V	M	A	A	G	F	I	A	D	T	A	S
Secondary structure
SS confidence
Disorder		?																																					?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		L	P	L	I	V	S	N	L	V	N	I	V	S	A	D	F	F	G	L	S	F	A	Q	Y	A	S	V	M	I	S	V	D	A	A	A	I	A	A	T	L	I	M	L	Y	L	F	F	R	R	V	I	P	A	T	Y	X	V	S	L	L
Secondary structure
SS confidence
Disorder				?	?																																													?	?		?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		K	T	P	A	S	A	I	K	D	L	A	T	F	R	A	G	W	I	V	L	L	L	L	L	V	G	F	F	F	L	E	P	Q	G	I	L	V	S	A	I	A	A	A	G	A	A	V	L	F	V	V	A	K	R	G	H	S	I	N	T
Secondary structure
SS confidence
Disorder			?	?	?	?	?	?	?	?	?																																												?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		G	K	V	L	R	G	A	P	W	Q	I	V	I	F	S	L	G	M	Y	I	V	V	Y	G	L	R	N	A	G	F	T	E	Y	L	S	G	V	L	N	L	L	A	D	K	G	L	W	A	A	T	F	G	T	G	F	L	T	A	F	L
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		S	S	V	M	N	N	M	P	T	V	L	I	G	A	L	S	I	D	G	S	T	A	T	G	V	V	K	E	A	M	I	Y	A	N	V	I	G	C	D	L	G	P	K	I	T	P	I	G	S	L	A	T	L	L	W	L	H	V	L	A
Secondary structure
SS confidence
Disorder																				?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.
Sequence		Q	K	N	M	T	I	T	W	G	Y	Y	F	R	T	G	I	V	M	T	V	P	V	L	F	V	T	L	A	A	L	A	W	R	L	S	V	T	L
Secondary structure
SS confidence
Disorder																																					?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 2w8a chain C Region: 2 - 130 Aligned: 117 Modelled: 109 Confidence: 76.5% Identity: 9% PDB header:membrane protein Chain: C: PDB Molecule:glycine betaine transporter betp; PDBTitle: crystal structure of the sodium-coupled glycine betaine2 symporter betp from corynebacterium glutamicum with bound3 substrate Phyre2 PDB 1j4n chain A Region: 16 - 124 Aligned: 107 Modelled: 107 Confidence: 30.1% Identity: 7% Fold: Aquaporin-like Superfamily: Aquaporin-like Family: Aquaporin-like Phyre2 PDB 3d9s chain B Region: 13 - 130 Aligned: 118 Modelled: 118 Confidence: 19.9% Identity: 9% PDB header:membrane protein Chain: B: PDB Molecule:aquaporin-5; PDBTitle: human aquaporin 5 (aqp5) - high resolution x-ray structure Phyre2 PDB 1ymg chain A Region: 13 - 121 Aligned: 109 Modelled: 109 Confidence: 17.3% Identity: 13% PDB header:membrane protein Chain: A: PDB Molecule:lens fiber major intrinsic protein; PDBTitle: the channel architecture of aquaporin o at 2.2 angstrom resolution Phyre2 PDB 1ymg chain A domain 1 Region: 13 - 121 Aligned: 109 Modelled: 109 Confidence: 17.3% Identity: 13% Fold: Aquaporin-like Superfamily: Aquaporin-like Family: Aquaporin-like Phyre2 PDB 2bbj chain B Region: 69 - 111 Aligned: 40 Modelled: 43 Confidence: 10.4% Identity: 15% PDB header:metal transport/membrane protein Chain: B: PDB Molecule:divalent cation transport-related protein; PDBTitle: crystal structure of the cora mg2+ transporter Phyre2 PDB 3rfr chain I Region: 57 - 148 Aligned: 92 Modelled: 92 Confidence: 5.7% Identity: 14% PDB header:oxidoreductase Chain: I: PDB Molecule:pmob; PDBTitle: crystal structure of particulate methane monooxygenase (pmmo) from2 methylocystis sp. strain m Phyre2 1 c2w8aC_ 2 d1j4na_ 3 c3d9sB_ 4 c1ymgA_ 5 d1ymga1 6 c2bbjB_ 7 c3rfrI_

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 c2w8aC_ 76.5 9 PDB header:membrane protein Chain: C: PDB Molecule:glycine betaine transporter betp; PDBTitle: crystal structure of the sodium-coupled glycine betaine2 symporter betp from corynebacterium glutamicum with bound3 substrate 2 d1j4na_ 30.1 7 Fold:Aquaporin-like Superfamily:Aquaporin-like Family:Aquaporin-like 3 c3d9sB_ 19.9 9 PDB header:membrane protein Chain: B: PDB Molecule:aquaporin-5; PDBTitle: human aquaporin 5 (aqp5) - high resolution x-ray structure 4 c1ymgA_ 17.3 13 PDB header:membrane protein Chain: A: PDB Molecule:lens fiber major intrinsic protein; PDBTitle: the channel architecture of aquaporin o at 2.2 angstrom resolution 5 d1ymga1 17.3 13 Fold:Aquaporin-like Superfamily:Aquaporin-like Family:Aquaporin-like 6 c2bbjB_ 10.4 15 PDB header:metal transport/membrane protein Chain: B: PDB Molecule:divalent cation transport-related protein; PDBTitle: crystal structure of the cora mg2+ transporter 7 c3rfrI_ 5.7 14 PDB header:oxidoreductase Chain: I: PDB Molecule:pmob; PDBTitle: crystal structure of particulate methane monooxygenase (pmmo) from2 methylocystis sp. strain m

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

Transmembrane helix prediction

Transmembrane helices have been predicted in your sequence to adopt the topology shown below

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0